Multiple-criteria decision-aiding framework to analyze and assess the governance of sustainability

Past and present disasters and scandals, such as the BP Deepwater Horizon oil disaster in the Gulf of Mexico in 2010, the Servier Mediator (Benfluorex) scandal in 2009 and the Enron collapse in 2001, have uncovered weaknesses in governance issues. The authors argue that there is a need to develop methods and tools to diagnose and assess the governance of organizations with respect to Sustainable Development (SD). However, this task remains difficult due to the fact that it is difficult to appraise the quality of governance. The authors propose a protocol to diagnose and analyze the governance of SD and explore the use of multiple-criteria decision-aiding methods to achieve this task. Two aggregation methods to assess the global governance are proposed: (1) The identification of a final governance index for an Organization. This method helps in establishing a global diagnosis of the quality of the governance of an Organization with respect to SD challenges. The governance index is based on the calculation of three indexes: the partial opportunity index, the partial risk index and the partial equilibrium index. (2) The ranking of a set of Organizations according to their governance of SD. This method aims at assessing a set of Organizations based on a pairwise comparison according to a set of criteria that represents the seven domains of the ISO 26000 norm (ISO 26000—Guidance on social responsibility, 2010). This method is based on the outranking aggregation approach ELECTRE III. A practical example is used to illustrate two methods of governance assessment.     

Optimisation of a PMAxx™-RT-qPCR Assay and the Preceding Extraction Method to Selectively Detect Infectious Murine Norovirus Particles in Mussels

Human noroviruses are a major cause for gastroenteritis outbreaks. Filter-feeding bivalve molluscs, which accumulate noroviruses in their digestive tissues, are a typical vector for human infection. RT-qPCR, the established method for human norovirus detection in food, does not allow discrimination between infectious and non-infectious viruses and can overestimate potentially infectious viral loads. To develop a more accurate method of infectious norovirus load estimation, we combined intercalating agent propidium monoazide (PMAxx™)-pre-treatment with RT-qPCR assay using in vitro-cultivable murine norovirus. Three primer sets targeting different genome regions and diverse amplicon sizes were used to compare one-step amplification of a short genome fragment to three two-step long-range RT-qPCRs (7 kbp, 3.6 kbp and 2.3 kbp amplicons). Following initial assays performed on untreated infectious, heat-, or ultraviolet-inactivated murine noroviruses in PBS suspension, PMAxx™ RT-qPCRs were implemented to detect murine noroviruses subsequent to their extraction from mussel digestive tissues; virus extraction via anionic polymer-coated magnetic beads was compared with the proteinase K-dependent ISO norm. The long-range RT-qPCR process detecting fragments of more than 2.3 kbp allowed accurate estimation of the infectivity of UV-damaged murine noroviruses. While proteinase K extraction limited later estimation of PMAxx™ pre-treatment effects and was found to be unsuited to the assay, magnetic bead-captured murine noroviruses retained their infectivity. Genome copies of heat-inactivated murine noroviruses differed by 2.3 log₁₀ between RT-qPCR and PMAxx™-RT-qPCR analysis in bivalve molluscs, the PMAxx™ pre-treatment allowing a closer approximation of infectious titres. The combination of bead-based virus extraction and PMAxx™ RT-qPCR thus provides a more accurate model for the estimation of noroviral bivalve mollusc contamination than the conjunction of proteinase K extraction and RT-qPCR and has the potential (once validated utilising infectious human norovirus) to provide an added measure of security to food safety authorities in the hazard assessment of potential bivalve mollusc contamination.

     

A morphological and structural study of the larval shell from the abalone <Emphasis Type="Italic">Haliotis tuberculata</Emphasis>

The larval shell of the marine gastropod Haliotis tuberculata was investigated by polarised light microscopy, scanning electron microscopy, Raman microspectroscopy and infra-red spectroscopy. Trochophore and veliger larval sections were used for histological examination of the growing shell and each larval stage was related to the shell development and the appearance of calcified formations. We determined the stage of initial calcification by specific staining combined with polarised light examination. The shell of 30-h-old pre-veliger larvae was found to be mineralized, confirming that calcification occurred before larval torsion. Using both infra-red and Raman spectroscopy, we showed that CaCO₃ deposition occurred at the pre-veliger stage and that the mineral phase initially deposited was essentially composed of aragonite.     

The swimming crab Charybdis smithii: distribution, biology and trophic role in the pelagic ecosystem of the western Indian Ocean

Surface “swarms” of the swimming crabs Charybdis smithii are still considered as an unusual phenomenon in the open Indian Ocean, although their dense pelagic aggregations were already reported in waters off the Indian coast and in the northern Arabian Sea. Based on an extensive large-scale data series taken over 45 years, we demonstrate that C. smithii is common in the pelagic provinces of the western Indian Ocean driven by the wind monsoon regime. Swimming crabs are dispersed by the monsoon currents throughout the equatorial Indian Ocean. They aggregate at night in the upper 150-m layer, where their estimated biomass derived from pelagic trawling data can exceed 130 kg km⁻². Abundance of C. smithii can reach >15,000 ind. km⁻² in July (i.e. the peak of the south-west monsoon), declines by 50-fold in March and is negligible in May. C. smithii is an important prey for more than 30 species of abundant epipelagic top predators. In turn, it feeds on mesopelagic species. This swimming crab is a major species of the intermediate trophic levels and represents a crucial seasonal trophic link in the open ocean ecosystem of the western Indian Ocean. Outbursts in pelagic waters of huge biomasses of ordinarily benthic crustaceans (C. smithii and Natosquilla investigatoris) are a remarkable feature of the Indian Ocean, although similar, but smaller, events are reported in the Pacific and Atlantic Oceans.

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Local disturbance cycles and the maintenance of heterogeneity across scales in marine metapopulations

In marine systems, the occurrence and implications of disturbance-recovery cycles have been revealed at the landscape level, but only in demographically open or closed systems where landscape-level dynamics are assumed to have no feedback effect on regional dynamics. We present a mussel metapopulation model to elucidate the role of landscape-level disturbance cycles for regional response of mussel populations to onshore productivity and larval transport. Landscape dynamics are generated through spatially explicit rules, and each landscape is connected to its neighbor through unidirectional larval dispersal. The role of landscape disturbance cycles in the regional system behavior is elucidated (1) in demographically open vs. demographically coupled systems, in relation to (2) onshore reproductive output and (3) the temporal scale of landscape disturbance dynamics. By controlling for spatial structure at the landscape and metapopulation levels, we first demonstrate the interaction between landscape and oceanographic connectivity. The temporal scale of disturbance cycles, as controlled by mussel colonization rate, plays a critical role in the regional behavior of the system. Indeed, fast disturbance cycles are responsible for regional synchrony in relation to onshore reproductive output. Slow disturbance cycles, however, lead to increased robustness to changes in productivity and to demographic coupling. These testable predictions indicate that the occurrence and temporal scale of local disturbance-recovery dynamics can drive large-scale variability in demographically open systems, and the response of metapopulations to changes in nearshore productivity.     

Prediction of extinction in plants: interaction of extrinsic threats and life history traits

The global extinction of species proceeds through the erosion of local populations. Using a 60-year time series of annual sighting records of plant species, we studied the correlates of local extinction risk associated with a risk of species extinction in the Park Grass Experiment where plants received long-term exposure to nutrient enrichment, soil acidification, and reductions in habitat size. We used multivariate linear models to assess how extrinsic threats and life history traits influence extinction risk. We investigated effects of four extrinsic threats (nitrogen enrichment, productivity, acidification, and plot size) as well as 11 life history traits (month of earliest flowering, flowering duration, stress tolerance, ruderalness [plant species' ability to cope with habitat disturbance], plant height, diaspore mass, seed bank, life form, dispersal mode, apomixis [the ability for a species to reproduce asexuall through seeds], and mating system). Extinction risk was not influenced by plant family. All of the 11 life history traits except life form and all threat variables influenced extinction risk but always via interactions which typically involved one threat variable and one life history trait. We detected comparatively few significant interactions between life history traits, and the interacting traits compensated for each other. These results suggest that simple predictions about extinction risk based on species' traits alone will often fail. In contrast, understanding the interactions between extrinsic threats and life history traits will allow us to make more accurate predictions of extinctions.     

The impact of human frequentation on coastal vegetation in a biosphere reserve

We aimed to assess the impacts of recreational trampling on rare species, plant communities and landscape structure in the Iroise Biosphere Reserve (western France). Focusing on coastal grasslands, we first identified indicators discriminating human-induced short grasslands (i.e. maintained short by intensive trampling) from natural short grasslands (i.e. maintained by environmental constraints): the presence of lichens and succulent or woody species, which are known to be highly sensitive to trampling, as well as a shallow soil were good indicators of natural short grasslands. Recreational activities affected the majority of plots containing rare species, but one third of rare species (according to their habitat preference) appeared currently not threatened by recreational activities. The other rare species were found in grasslands with low trampling intensity and were not found in grasslands with greater trampling intensity. One lichen species (Teloshistes flavicans) was not affected by trampling intensity, while two plants species (Scilla verna and Ophioglossum lusitanicum) showed higher abundances when trampling was low to medium. When it occurs in natural short grasslands, tourist trampling reduced drastically plant species richness. However, when considering maritime high grasslands, we observed that species richness was higher under low trampling vs. no trampling, but decreased at higher trampling intensity. At a landscape scale, the mean annual rate of path creation was about 1.6% and tourist trampling has already completely destroyed 3.5ha of natural coastal vegetation. Trampling of maritime-high grassland has also created 31ha of short grasslands, which represent 50.8% of the whole short grassland habitat of the island. Moreover trampling affected respectively, 41% and 15% of natural short grasslands and maritime-high grasslands. One of the main suggestions for managers to minimise trampling impacts should be to protect areas of rocky soil covered by short grassland that are still non-trampled and not impacted. Fortunately, this appears compatible with a relatively free access of visitors to coastal areas, as tourists can be redirected towards maritime-high grasslands, an habitat which is less impacted by tourism in terms of affected surface, soil cover, loss of species, or presence of rare species host.     

Nitrogen and metals in two regions in Central Europe: Significant differences in accumulation in mosses due to land use?

The study was conducted to test the hypothesis that the regional variability of nitrogen (N) and metal accumulations in terrestrial ecosystems are due to historical and recent ways of landuse. To this end, two regions of Central Europe were selected for investigation: the Weser-Ems Region (WER) and the Euro Region Nissa (ERN). They were assumed to have land use-specific accumulation profiles. Thus, the metal and N accumulations in both regions were examined by means of geostatistically based comparative moss analysis. The sampling and chemical analysis of mosses were conducted in accordance with the convenient guidelines and methods, respectively. The spatial representativity of the sampling sites was computed by means of a land classification which was calculated for Europe by means of classification trees and GIS-techniques. The differences of deposition loads were tested for statistical significance with regard to time and space. The measurement values corroborated the decline of metal accumulation observed since the beginning of the European Metals in Mosses Surveys in 1990. The metal loads of the mosses in the ERN exceeded those in the WER significantly. The opposite holds true for the N concentrations: those in the WER were significantly higher than those in the ERN. The reduction of emissions from power plants, factories and houses was strongly correlated with the decline of deposition and bioaccumulation of metals. As proved by the European Metals in Mosses Surveys, this tendency is due to successful environmental policies. But no such success could be verified by monitoring the accumulation of N in mosses.     

Das Biologische System der Elemente (BSE): Eine modelltheoretische Betrachtung zur Essentialität von chemischen Elementen

The correlation of data on the elements obtained by instrumental chemical analysis of highly representative plant samples led to the establishment of a first Biological System of Elements (BSE) in 1994. Including other physiological parameters like availability of chemical elements, transport, storage, etc. it is clear, that aside from the bioinorganic aspects of coordination chemistries of given elements, features of essentiality or toxicity depend on their interference with enzymatic processes. Notably, the latter are not to be considered as purely catalytic transformations, but are instead related to the reproduction of organisms in a direct or indirect manner — as autocatalytic: a process whereby a protein containing some metal or burdened in function by its presence is indirectly involved in its own reproduction. Stoichiometric Network Analysis (SNA) explicitly deals with the general dynamics of such autocatalytic systems. Given there is a relationship between the kinds of metal or metalloid species and the key biological/biochemical transformations to be promoted using them — a relationship which is the topic of bioinorgnaic chemistry-, and that biochemistry is in effect about systems which can reproduce and thus behave autocatalytically, one can expect SNA to yield statements on the basic features of biology and biochemistry as well.     

Navigating protected areas as social-ecological systems: integration pathways of French nature reserves

Regional Environmental Change - On a global scale, protected areas (PAs) are one of the main tools used for biodiversity conservation. However, accelerated biodiversity loss and lack of social...