Comparison of the complexation potential of extracellular polymeric substances (EPS), extracted from activated sludges and produced by pure bacteria strains, for cadmium, lead and nickel

This paper provides information on the metal complexation potential of extracellular polymeric substances (EPS), extracted from activated sludges and from eight pure cultures of bacteria isolated from the same activated sludge. The EPS extracted from pure bacteria cultures are mainly composed of proteins and low quantities of polysaccharides and uronic acids in comparison with EPS extracted from activated sludges. The EPS studied present two apparent pK(a) and the IR spectra show the presence of the same functional groups on all the EPS studied. The ability of EPS to complex Cd, Pb and Ni, was studied at pH 7 with Chau and Ruzic's models using polarography titration. All of the EPS exhibited a greater ability to complex Pb than Ni, Cd showing the weakest affinity overall. The EPS extracted from the pure cultures of bacteria were less able to complex the metals than that extracted from activated sludges. Literature data, IR data and EPS phosphorous content, supported by the EPS pK(a), revealed that carboxylic and phosphoric groups may play a major role in binding to metals at pH 7. This study underlines the importance of metal exposure in order for bacteria to secrete or modify EPS. After exposure, the EPS then exhibit the greatest capacity to bind metal in order to protect bacteria from harmful effects of heavy metals.     

Investigation on the chemical structure of nonextractable residues of the fungicide cyprodinil in spring wheat using 13C-C1-phenyl-cyprodinil on 13C-depleted plants-an alternative approach to investigate nonextractable residues

13C-labelled cyprodinil was applied on 13C-depleted wheat plants with 27-fold field application rate. A control experiment applying same amounts of 14C-cyprodinil showed that main portions of the residues were detected in the cellulose (15% NER), hemicellulose (28.3% NER), and lignin fraction (23.3% NER). 16.7% were detected in water soluble polymers, 6% in both, pectin and protein fraction, and 4% in the starch containing fraction. Free cyprodinil was detectable by TLC in all fractions except lignin. A direct characterization of the residues in vivo by CP-MAS was not successful. Cell wall fractions were further analysed by liquid state NMR to determine the structure of the mobilized highly polymer/polar residues: Within lignin, where most of the residues were located at field application rate, neither intact cyprodinil nor its metabolites could not be detected. The 13C-label introduced was probably incorporated in the polymer as natural lignin monomers and thus are not considered as bound residues according to IUPAC definition.     

Neural Network Input Selection for Hydrological Forecasting Affected by Snowmelt1

Abstract: Snowmelt largely affects runoff in watersheds in Nordic countries. Neural networks (NN) are particularly attractive for streamflow forecasting whereas they rely at least on daily streamflow and precipitation observations. The selection of pertinent model inputs is a major concern in NNs implementation. This study investigates performance of auxiliary NN inputs that allow short‐term streamflow forecasting without resorting to a deterministic snowmelt routine. A case study is presented for the Rivière des Anglais watershed (700 km²) located in Southern Québec, Canada. Streamflow (Q), precipitations (rain R and snow S, or total P), temperature (T) and snow lying (A) observations, combined with climatic and snowmelt proxy data, including snowmelt flow (Q_SM) obtained from a deterministic model, were tested. NN implemented with antecedent Q and R produced the largest gains in performance. Introducing increments of A and T to the NNs further improved the performance. Long‐term averages, seasonal data, and Q_SM failed to improve the networks.     

Conservation value of an exotic species: the case of coconuts on the Kayapo indigenous lands, south-eastern Amazonia

In a meeting between FUNAI (the Brazilian Indian National Foundation) and the Kayapo in the Kayapo village of Aukre, in October 2005, the Kayapo Indians requested support to increase the culture of coconuts (Cocos nucifera) in their lands. The introduction of exotic species is a cause of biological diversity loss throughout the world. However, this is by no means applicable to all kinds of exotic species. We argue that the culture of coconuts may actually be a useful tool for the conservation of this country’s indigenous lands, despite criticism to the contrary. It may also be useful as source for raw material for the indigenous people who are presently experiencing a population boom. For the Kayapo of south-eastern Amazonia, such a demographic explosion, coupled with their increasing dependence on money to purchase industrialized goods, tend to substantially increase the pressure on their lands, which still contain a substantial amount of well-preserved seasonally-dry Amazonian forests. We investigated the actual economic relevance of coconuts for the Kayapo by measuring human and coconut populations and found that this exotic palm is not important for the subsistence of these Indians, due to coconut scarcity, and that such importance is not forthcoming. In the Amazon, coconuts may be particularly useful for the Indians’ nutrition due to the high energetic value of the solid endosperm of the mature fruit. Therefore, given the importance of indigenous lands for the conservation of the Amazonian forests, the encouragement of subsistence coconut cultures in indigenous lands is defensible as a measure for the conservation of biodiversity.     

A New Test Method for Determining Biodegradation of Plastic Material Under Controlled Aerobic Conditions in a Soil-Simulation Solid Environment

A new test method is described for assessing biodegradation of plastic material under simulated soil conditions. An inert substrate can be activated with soil extract and nutrient and used in place of soil in biodegradation tests. The biodegradation level is evaluated by determining the carbon dioxide (CO₂) production released by the test reactors. Effects of substrate nature, solution pH, nutrient composition, soil extract concentration, and activation duration on CO₂ production were investigated, and the experimental conditions were optimized. Results obtained with cellulose showed a biodegradation rate of 80% within 28 days. Moreover, with this kind of substrate, reaction products and residues can be easily extracted and analysed.     

Tissues and hair residues and histopathology in wild rats (Rattus rattus L.) and Algerian mice (Mus spretus Lataste) from an abandoned mine area (Southeast Portugal)

Data gathered in this study suggested the exposure of rats and Algerian mice, living in an abandoned mining area, to a mixture of heavy metals. Although similar histopathological features were recorded in the liver and spleen of both species, the Algerian mouse has proved to be the strongest bioaccumulator species. Hair was considered to be a good biological material to monitor environmental contamination of Cr in rats. Significant positive associations were found between the levels of this element in hair/kidney (r=0.826, n=9, p<0.01) and hair/liver (r=0.697, n=9, p=0.037). Although no association was found between the levels of As recorded in the hair and in the organs, the levels of this element recorded in the hair, of both species, were significantly higher in animals captured in the mining area, which met the data from the organs analysed. Nevertheless, more studies will be needed to reduce uncertainty about cause-effect relationships.     

Effect of textile auxiliaries on the biodegradation of dyehouse effluent in activated sludge

The textile industry is confronted with serious environmental problems associated with its immense wastewater discharge, substantial pollution load, extremely high salinity, and alkaline, heavily coloured effluent. Particular sources of recalcitrance and toxicity in dyehouse effluent are two frequently used textile auxiliaries; i.e. dye carriers and biocidal finishing agents. The present experimental work reports the observation of scientific and practical significance related with the effect of two commercially important textile dye carriers and two biocidal finishing agents on biological activated sludge treatment at a textile preparation, dyeing and finishing plant in Istanbul. Respirometric measurements of the dyehouse effluent spiked with the selected textile chemicals were carried out for the assessment of the "readily biodegradable COD fraction" of the wastewater. The respirometric data obtained to visualize the effect of the selected textile auxiliaries on biomass activity was evaluated by an adopted activated sludge model. Results have indicated that the tested biocides did not exert any significant inhibitory effect on the treatment performance of the activated sludge reactor at the concentrations usually encountered in the final, total dyehouse effluent. The situation with the dye carriers was inherently different; one dye carrier appeared to be highly toxic and caused serious inhibition of the microbial respirometric activity, whereas the other dye carrier, also known as the more ecological alternative, i.e. the "Eco-Carrier", appeared to be biodegradable. Finally, the respirometric profile obtained for the Eco-Carrier was described by a simplified respirometric model.     

Effects of pyrene on mussels in different experimental conditions

The toxicity of the polyaromatic hydrocarbon pyrene to Mytilus edulis and Mytilus galloprovincialis was investigated in experiments conducted in the United Kingdom for M. edulis and in Turkey for M. galloprovincialis. Experimental conditions of temperature and salinity were chosen to be appropriate to the ambient conditions in which the mussels typically live. The effect of different feeding regimes on pyrene bioaccumulation and toxicity was also investigated. Feeding rate and neutral red retention biomarker techniques were used for toxicity assessment. An experiment with M. edulis demonstrated that mussels exposed to pyrene accumulated increasing amounts of this compound throughout a 15-day exposure period and that accumulation increased in relation to exposure concentration and with increasing concentration of unicellular algal food material. However, in these experiments, which were protected from UV light, there was no clear relationship between pyrene concentration in tissues and feeding rate. A clear concentration-response relationship was observed between tissue concentration and neutral red retention for days 1 and 7 of the experiment, but this relationship was lost by day 15, with evidence of the cells recovering. A similar experiment was conducted with M. galloprovincialis at a single (high) pyrene concentration in darkness. The feeding rate of the exposed mussels was always lower than the feeding rate of the control mussels, although the difference was insignificant at 7-day exposure. A decreasing trend in health status of the mussels was indicated by the neutral red retention assay results after 7 and 15 days of exposure to pyrene. In a third experiment (with M. galloprovincialis), an illuminated algal chemostat system containing pyrene was inserted in the exposure system as the food source for the mussels. Both biomarker results showed conclusively that toxic effects correlated with pyrene bioaccumulation, though there was no clear evidence for light-enhanced toxicity. These experiments showed that experimental conditions such as salinity, temperature and feeding regime and perhaps the species of mussel used may influence whether pyrene is perceived as being a toxic molecule.     

Lead and cadmium biosorption by extracellular polymeric substances (EPS) extracted from activated sludges: pH-sorption edge tests and mathematical equilibrium modelling

The sorption of Cd and Pb by extracellular polymeric substances (EPS) extracted from activated sludges originated from wastewater treatment plants (WWTPs) or Lab-scale bioreactors was investigated as a function of pH. The study was carried out using a polarographic method in the SMDE (stripping mercury dropping electrode) mode which is suited to determine labile metals in solution containing soluble ligands such as EPS. The results obtained provide evidence of the presence of a pH-sorption/desorption edge for Cd and Pb by EPS. The use of Kurbatov’s model gives information on the mechanisms involved through the determination of “relative complexation constants” (operationally defined) and the number of protons exchanged. The use of this model demonstrates that proton exchange with metals is not the only mechanism involved in metal biosorption by EPS. Other mechanisms such as cation exchange with Ca or Mg, global electric field surrounding the ligand or micro-precipitation of metals could be involved in metal sorption by EPS. The position of the pH-sorption edge curves and the “relative complexation constants” show that Pb displays a greater affinity for EPS than Cd. The studied EPS have large differences regarding binding strength of Cd and Pb. These differences are not correlated with the organic parameters measured to characterize the EPS, however the mineral fraction of the EPS could be involved to a large extent in the sorption of metal.