Asexual viviparity and population genetics of Actinia tenebrosa

The intertidal anemone Actinia tenebrosa is viviparous. An electrophoretic study of 3 polymorphic enzymes in Western Australian populations has confirmed genetic identity of adults and their brood young, indicating asexual reproduction. The population effects of this clonal reproduction are seen as linkage disequilibrium and departures from Hardy-Weinberg equilibrium within populations, and large differences between populations. The data also suggest occasional sexual reproduction, and the likelihood of a mixture of reproductive modes in this species.     

Living dangerously: driver distraction at high speed

Recent research indicates that cell phone use can distract drivers from safe vehicle operation. However, estimates of the prevalence of cell phone use while driving have been limited to daytime hours and low-speed roadways. This paper describes the results of a study to estimate rates of cell phone use and other distractions by examining approximately 40,000 high-quality digital photographs of vehicles and drivers on the New Jersey Turnpike. The photographs, which originally were collected as part of a separate study, were taken both during the day and during the night and at different locations across the span of the Turnpike. A radar gun linked to the camera recorded the speeds of vehicles as they passed. This provided us with the speeds of every vehicle photographed, and allowed us to determine population counts of vehicles. A panel of three trained coders examined each photograph and recorded the presence of cell phone use by the drivers or any other distracting behavior. Demographic information on the driver was obtained during previous examinations of the photographs for an unrelated study. A rating was considered reliable when two out of the three coders agreed. Population estimates (and confidence intervals) of cell phone use and other distractions were estimated by weighting the cases by the inverse probability of vehicle selection. Logistic regression was used to predict cell phone use from demographic and situational factors. The results indicated that the most frequent distraction was cell phone use: 1.5% of the drivers on the Turnpike were using cell phones compared to the 3 to 4% use rates reported in the National Occupant Protection Use Survey (NOPUS) surveys conducted during the daytime on lower speed roadways. The Turnpike survey indicated that cell phones were used less on weekends and at night, and when the driver was exceeding the speed limit or had a passenger in the car.     

Ammonia volatilization from marsh-pond-marsh constructed wetlands treating swine wastewater

Ammonia (NH3) volatilization is an undesirable mechanism for the removal of nitrogen (N) from wastewater treatment wetlands. To minimize the potential for NH3 volatilization, it is important to determine how wetland design affects NH3 volatilization. The objective of this research was to determine how the presence of a pond section affects NH3 volatilization from constructed wetlands treating wastewater from a confined swine operation. Wastewater was added at different N loads to six constructed wetlands of the marsh-pond-marsh design that were located in Greensboro, North Carolina, USA. A large enclosure was used to measure NH3 volatilization from the marsh and pond sections of each wetland in July and August of 2001. Ammonia volatilized from marsh and pond sections at rates ranging from 5 to 102 mg NH3-N m(-2) h(-1). Pond sections exhibited a significantly greater increase in the rate of NH3 volatilization (p < 0.0001) than did either marsh section as N load increased. At N loads greater than 15 kg ha(-1) d(-1), NH3 volatilization accounted for 23 to 36% of the N load. Furthermore, NH3 volatilization was the dominant (54-79%) N removal mechanism at N loads greater than 15 kg ha(-1) d(-1). Without the pond sections, NH3 volatilization would have been a minor contributor (less than 12%) to the N balance of these wetlands. To minimize NH3 volatilization, continuous marsh systems should be preferred over marsh-pond-marsh systems for the treatment of wastewater from confined animal operations.     

Selenium stable isotope ratios in California agricultural drainage water management systems

Selenium stable isotope ratios are known to shift in predictable ways during various microbial, chemical, and biological processes, and can be used to better understand Se cycling in contaminated environments. In this study we used Se stable isotopes to discern the mechanisms controlling the transformation of oxidized, aqueous forms of Se to reduced, insoluble forms in sediments of Se-affected environments. We measured 80Se/76Se in surface waters, shallow ground waters, evaporites, digested plants and sediments, and sequential extracts from several sites where agricultural drainage water is processed in the San Joaquin Valley of California. Selenium isotope analyses of samples obtained from the Tulare Lake Drainage District flow-through wetland reveal small isotopic contrasts (mean difference 0.7%) between surface water and reduced Se species in the underlying sediments. Selenium in aquatic macrophytes was very similar isotopically to the NaOH and Na2SO3 sediment extracts designed to recover soluble organic Se and Se(0), respectively. For the integrated on-farm drainage management sites, evaporite salts were slightly (approximately 0.6%) enriched in the heavier isotope relative to the inferred parent waters, whereas surface soils were slightly (approximately 1.4%) depleted. Bacterial or chemical reduction of Se(VI) or Se(IV) may be occurring at these sites, but the small isotopic contrasts suggest that other, less isotopically fractionating mechanisms are responsible for accumulation of reduced forms in the sediments. These findings provide evidence that Se assimilation by plants and algae followed by deposition and mineralization is the dominant transformation pathway responsible for accumulation of reduced forms of Se in the wetland sediments.     

Spatial extrapolation of soil characteristics using whole-soil particle size distributions

Soils support ecosystem functions such as plant growth and water quality because of certain physical, chemical, and biological properties. These properties have been studied at different spatial scales, including point scales to satisfy basic research needs, and regional scales to satisfy monitoring needs. Recently, soil property data for the entire USA have become available in the State Soil Geographic Data Base (STATSGO), which is appropriate for regional-scale research. We analyzed and created models of STATSGO data in this study to serve as a research tool, for example, for linking the soil to regional water quality monitoring data in our companion paper. Map units in STATSGO define geographic land areas by soil characteristics (SCs) of similar soil series. We selected 27 SCs that influenced water properties (in varying degrees), aggregated the layer and component SCs to map unit SCs, and used SCs to calculate relationships among map units. The relationships were defined by equations of conditional mean for the qth SC (SCq), while using the remaining 26 SCs as predictors. The relative standard errors for 22 of the 27 SCs were less than 10%, and less than 22% for the remaining five. We conclude that spatial extrapolation of SCs is feasible and the procedures are a first step toward extrapolating information across a region using SC-water property relationships. Although our procedure is for regional scale monitoring, it is also applicable to finer spatial scales commensurate with available soil data.     

Behaviour and transport of radionuclides in soil and vegetation of a sand dune ecosystem

A sand dune ecosystem in the vicinity of the British Nuclear Fuels reprocessing plant at Sellafield, Cumbria, UK was used to examine the spatial, temporal and depth distributions of 134Cs, 137Cs, 238Pu, 239 + 240Pu and 241Am in soil and in two species of vegetation (Festuca rubra, Ammophila arenaria). Core samples showed evidence of the accumulation of radionuclides derived mainly from sea-to-land transfer. Accumulated deposits of radioactivity (0-0.1 m) lie within the range: 1.1-3.4 Bq kg-1 (134Cs), 260-440 Bq kg-1 (137Cs), 31-40 Bq kg-1 (238Pu), 150-215 Bq kg-1 (239 + 240Pu) and 190-240 Bq kg-1 (241Am). Soil profiles showed greater activity concentrations in their deeper regions and this is attributed to leaching of radionuclides in percolating drainage water accentuated by the coarse texture, low organic matter and clay mineral content of coastal sands. Radionuclide activity concentrations in F. rubra and A. arenaria were similar, in the ranges 20-70 Bq kg-1 (137Cs), 1-5 Bq kg-1 (238Pu), 10-30 Bq kg-1 (239 + 240Pu) and 10-65 Bq kg-1 (241Am). Clear temporal and spatial variations were observed in both species of vegetation, reflecting the weather conditions antecedent to the sampling period and the influence of sea-to-land transfer. Concentration ratios (vegetation:soil) for activity concentrations in the two species were similar, in the ranges: 0.05-0.14 (137Cs), 0.025-0.097 (238Pu), 0.022-0.057 (239 + 240Pu) and 0.025-0.212 (241Am).     

Toward efficient riparian restoration: integrating economic, physical, and biological models

This paper integrates economic, biological, and physical models to explore the efficient combination and spatial allocation of conservation efforts to protect water quality and increase salmonid populations in the Grande Ronde basin, Oregon. We focus on the effects of shade on water temperatures and the subsequent impacts on endangered juvenile salmonid populations. The integrated modeling system consists of a physical model that links riparian conditions and hydrological characteristics to water temperature; a biological model that links water temperature and riparian conditions to salmonid abundance, and an economic model that incorporates both physical and biological models to estimate minimum cost allocations of conservation efforts. Our findings indicate that conservation alternatives such as passive and active riparian restoration, the width of riparian restoration zones, and the types of vegetation used in restoration activities should be selected based on the spatial distribution of riparian characteristics in the basin. The relative effectiveness of passive and active restoration plays an important role in determining the efficient allocations of conservation efforts. The time frame considered in the restoration efforts and the magnitude of desired temperature reductions also affect the efficient combinations of restoration activities. If the objective of conservation efforts is to maximize fish populations, then fishery benefits should be directly targeted. Targeting other criterion such as water temperatures would result in different allocations of conservation efforts, and therefore are not generally efficient.