Effect of tea saponin on phytoremediation of Cd and pyrene in contaminated soils by <Emphasis Type="Italic">Lolium multiflorum</Emphasis>

Tea saponin (TS), a kind of green biosurfactant produced by plants, was added into the Cd–pyrene co-contaminated soils to evaluate its influence on phytoremediation of Cd and pyrene by Lolium multiflorum. The results showed that the accumulation of pyrene in L. multiflorum was significantly promoted by the TS. Compared with no TS treatments (PL and ML), the aboveground concentrations of pyrene in TS treatments (PLT and MLT) increased by 135 and 30%, respectively, and the underground concentrations of pyrene in TS treatments (PLT and MLT) increased by 40 and 25%. The concentrations of Cd in the aboveground and underground parts in single contaminated treatments were all significantly more than those in co-contaminated treatments, while the situation of pyrene was quite the reverse. Besides, the addition of TS enhanced activities of dehydrogenase and polyphenol oxidase in soils and increased the biomass of L. multiflorum. The micromorphology of L. multiflorum was not affected by TS. The study suggests that the use of L. multiflorum with TS is an alternative technology for remediation of Cd–pyrene co-contaminated soils.     

Evaluating knowledge integration and co-production in a 2-year collaborative learning process with smallholder dairy farmer groups

Although knowledge integration and co-production are integral to transdisciplinary approaches to foster sustainable change in social–ecological systems, this type of research is usually not evaluated based on assessments of the learning process. While participants are meant to be central in such approaches, too often, their perspectives are not central to the evaluation. Moreover, there is limited empirical information about how new knowledge is transformed into action. We respond to these knowledge gaps by analyzing (A) farmers’ perspectives on the collaborative learning process and (B) how farmers’ new knowledge can serve as the basis for changed actions. Theoretically, we are guided by second-order cybernetics and have integrated the Control Loop Model with Learning Loops to extend Kirkpatrick (Evaluating training programs: the four levels, 2nd edn. Berrett-Koehler Publisher, San Francisco, 1998) four-level evaluation scheme. We apply this to evaluate a 2-year collaborative learning process with two smallholder dairy farmer groups in Nakuru County, Kenya that aimed to co-develop local sustainable pathways to reduce milk losses. Results showed that farmers learned by (1) implementing corrective actions based on known cause–effect relations (single-loop learning); (2) discovering new cause–effect relations and testing their effect (double-loop learning); and (3) further questioning and changing their aims (triple-loop learning). Highlighting the importance of knowledge integration and co-production, this collaboration between farmers, researchers, and field assistants improved the farmers’ ability to respond, adapt, and intentionally transform their farming system in relation with complex sustainability challenges. Results demonstrate that the potential of our evaluation scheme to better reflect learning and empowerment experienced by actors involved in transdisciplinary research for sustainability.     

Species richness,structure, and conservation of <Emphasis Type="Italic">Nitraria retusa</Emphasis> communities in the coastal salt marshes of Kuwait

Although the coastal salt marshes of Arabian Gulf have been altered extensively by human development activities, there is a paucity of data describing changes in the distribution and abundance of native coastal plant communities. The main objectives of this study are to determine vegetation condition, size structure, and conservation status of Nitraria retusa, a medicinal and salt-tolerant shrub, in disturbed and non-disturbed coastal salt marshes of Kuwait. Size measurements of Nitraria shrubs and nabkas, which are mounds of sediment developed around shrubs, were carried out in 50 quadrats (20 × 20 m²), randomly selected inside and outside Sabah Al-Ahmad Natural Reserve. Species richness and soil properties of nabkas and interspaces, the open areas between the nabkas, were also measured. The results revealed that nabkas of Nitraria in non-disturbed sites are more stable and rich in plant diversity than those in disturbed sites. Mean height and mean canopy diameter of Nitraria shrubs, total plant cover, and species richness are significantly higher in non-disturbed sites than disturbed sites, which indicate the positive influences of conservation for long term on vegetation structure and species richness. The results of soil analyses indicate the important role of nabkas in providing refuges for plant life and species diversity. The present study indicates that more than 50 % of the N. retusa community has been lost during the last few decades. Therefore, N. retusa should be considered an endangered species in Kuwait. The reduction in vegetation cover, a decline in species richness, and the overall degradation of salt marshes are attributed to human development activities along the coast of Kuwait. Effective conservation actions for threatened species in degraded coastal salt marshes of this region include establishment of protective enclosures, prohibitions on development that adversely affects native plant communities, and the planting native salt-tolerant shrubs to facilitate regeneration.     

Gendered knowledge and adaptive practices: Differentiation and change in Mwanga District,Tanzania

We examine the wider social knowledge domain that complements technical and environmental knowledge in enabling adaptive practices through two case studies in Tanzania. We are concerned with knowledge production that is shaped by gendered exclusion from the main thrusts of planned adaptation, in the practice of irrigation in a dryland village and the adoption of fast-maturing seed varieties in a highland village. The findings draw on data from a household survey, community workshops, and key informant interviews. The largest challenge to effective adaptation is a lack of access to the social networks and institutions that allocate resources needed for adaptation. Results demonstrate the social differentiation of local knowledge, and how it is entwined with adaptive practices that emerge in relation to gendered mechanisms of access. We conclude that community-based adaptation can learn from engaging the broader social knowledge base in evaluating priorities for coping with greater climate variability.     

Sperm viability staining in ecology and evolution: potential pitfalls

The causes and consequences of variation in sperm quality, survival and ageing are active areas of research in ecology and evolution. In order to address these topics, many recent studies have measured sperm viability using fluorescent staining. Although sperm viability staining has produced a number of interesting results, it has some potential pitfalls that have rarely been discussed. In the present paper, I review the major findings of ecology and evolution studies employing sperm viability staining and outline the method’s principle limitations. The key problem is that the viability assay may itself kill sperm, which is likely to confound many common experimental designs in addition to producing artificially low estimates of sperm viability. I further suggest that sperm number should be routinely measured in sperm viability studies, as it may be an important but overlooked source of spurious results. I provide methodological advice on sperm viability staining aimed at minimising artefacts and producing robust conclusions, and discuss possible avenues for future research. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users.     

Mid-21st century climate and weather extremes in Cyprus as projected by six regional climate models

We present climate change projections and apply indices of weather extremes for the Mediterranean island Cyprus using data from regional climate model (RCM) simulations driven by the IPCC A1B scenario within the ENSEMBLES project. Daily time-series of temperature and precipitation were used from six RCMs for a reference period 1976–2000 and for 2026–2050 (‘future‘) for representative locations, applying a performance selection among neighboring model grid-boxes. The annual average temperatures of the model ensemble have a ±1.5°C bias from the observations (negative for maximum and positive for minimum temperature), and the models underestimate annual precipitation totals by 4–17%. The climatological annual cycles for the observations fall within the 1σ range of the 6-model average, highlighting the strength of using multi-model output. We obtain reasonable agreement between models and observations for the temperature-related indices of extremes for the recent past, while the comparison is less good for the precipitation-related extremes. For the future, the RCM ensemble shows significant warming of 1°C in winter to 2°C in the summer for both maximum and minimum temperatures. Rainfall is projected to decrease by 2–8%, although this is not statistically significant. Our results indicate the shift of the mean climate to a warmer state, with a relatively strong increase in the warm extremes. The precipitation frequency is projected to decrease at the inland Nicosia and at the coastal Limassol, while the mountainous Saittas could experience more frequent 5–15 mm/day rainfall. In future, very hot days are expected to increase by more than 2 weeks/year and tropical nights by 1 month/year. The annual number of consecutive dry days shows a statistically significant increase (of 9 days) in Limassol. These projected changes of the Cyprus climate may adversely affect ecosystems and the economy of the island and emphasize the need for adaptation strategies.     

Development impact of advocacy initiatives in solid waste management in Nigeria

Solid waste management (SWM) in many low- and middle-income countries is sometimes driven by the informal sector. Unfortunately, contributions of the sector to SWM are not acknowledged in many developing countries. This situation often arises from inadequate awareness and lack of advocacy for these development contributions. This article reports on the impact of a study conducted by final-year geography undergraduates on the informal waste management sector in Nsukka urban area, Nigeria. The purposes of this exercise were to stimulate development intervention on behalf of the sector to improve perceptions, attitudes and performance, to impart a range of development research skills and to expose the students to the business opportunities provided by SWM and recycling. Informal waste sector workers and government officials responsible for SWM were interviewed, and some commercial data on recycling operations were collected. Findings of this study indicate that such advocacy initiatives were effective in increasing students’ awareness of opportunities and interest in working in the informal waste sector to promote better SWM and development in Nigeria. Lessons from this university case study are also drawn for other developing countries struggling to achieve the poverty reduction and job creation Millennium Development Goals (MDGs).     

Plant Species Richness and Developmental Morphology Stage Influence Mycorrhizal Patagonia Plants Root Colonization

The objectives of this study were to determine the percentage of root colonization by arbuscular mycorrhizal (AM) fungi at various levels of plant species richness and developmental morphology stages in various perennial grass, and herbaceous and woody dicots species using experimental plots during 2013 and 2014. An auger was used to obtain six replicate root + soil samples at each sampling time on each of the study parameters. Roots were washed free of soil, and percentage AM was determined. The shrub Larrea divaricata was the species which showed the lowest percentage of colonization by AM at the vegetative developmental morphology stage at the monocultures and six-species-mixtures on the experimental plots. Dicots, but not grass, species showed a greater percentage colonization by AM fungi at the greatest (i.e., six-species-mixtures) than lowest (i.e., monocultures) species richness. Although at different degrees of species richness and developmental morphology stages, the perennial grasses Nassella longiglumis and N. tenuis, the herbaceous dicot Atriplex semibaccata, and the shrubs L. divaricata and Schinus fasciculatus showed a greater (p < 0.050) percentage colonization by AM fungi during the second than the first study year. Even though it was species- and sampling time-dependent, percentage colonization by AM fungi increased as species richness also increased most of the times. Our results demonstrated that the plant species differences in percentage colonization by AM fungi in the experimental plots were species richness-, developmental morphology stage-, and sampling-time dependents.     

Climatic changes and their impact on socio-economic sectors in the Bhutan Himalayas: an implementation strategy

This paper contributes to an enhanced understanding of present climatic conditions, observed climate trends and regional climate vulnerability of the Bhutan Himalayas. Bhutan’s complex, often high-altitude terrain and the severe impact of the Indian summer monsoon leads to a strong exposure of the countries’ key economic sectors (agriculture, forestry, hydropower generation and tourism) to climatic changes. Climate change also threatens Bhutan’s vast biodiversity and increases the likelihood of natural hazards (e.g. glacier lake outburst floods, flash floods, droughts and forest fires). A better understanding of Bhutan’s climate and its variability, as well as observed and possible climate impacts, will help in improving the handling of regional social, economic and ecologic challenges not limited to the Himalayas. Only a few climatological studies exist for the eastern Himalayas. They mainly focus on adaptation to immediate threats by glacier lake outbursts. In contrast, this paper (1) investigates the average spatial and inner-annual diversity of the air temperature regime of Bhutan, based on local meteorological observations, (2) discusses past temperature variability, based on global datasets, and (3) relates effects of observed warming to water availability, hydropower development, natural hazards, forests, biodiversity, agriculture, human health and tourism in the Bhutan Himalayas. Results indicate a large spatial and temporal temperature variability within Bhutan and considerably increasing temperatures especially over recent decades. Implications of regional climatic changes on various socio-economic sectors and possible adaptation efforts are discussed.