Wave hydrodynamics around a multi-functional artificial reef at Leirosa

This paper describes an application of the Boussinesq-type COULWAVE model to study the wave hydrodynamics in the vicinity of a multi-functional artificial reef (MFAR). This reef is under investigation and consists of a supplementary protection solution for the Leirosa sand dune system located at South of Figueira da Foz, on the Portuguese West coast. Such installation near the coastline is expected to contribute to enhance the surfing conditions in the area, protect the sand dune system in the surroundings of Leirosa beach, and increase its environmental value. Numerical calculations with the COULWAVE model were performed for four test cases, considering two reef geometries (differing in the reef angle) and two incident wave conditions (storm condition and a common wave condition). Comparisons between the results obtained, in terms of wave heights and breaking line positions allow us to assess the influence of the reef on the hydrodynamics near the beach and around the reef. Moreover, the reef performance was analysed in terms of surfability and coastal protection. The surfability parameters (breaker height, Iribarren number and peel angle) were calculated for each test case using the numerical wave heights, wave directions and wave breaking positions. Comparisons of parameters allow characterizing the most appropriate configuration of the reef to improve the surfing conditions in the study area. A methodology based on numerical free surface elevations and horizontal velocity components was developed to calculate wave directions, since this is not a direct output of the COULWAVE model. Concerning coastal protection, analyses of the mean currents around the reef were used together with observations of the velocity cells near the shoreline as an indication of the sediment transport.     

Determination of seventeen endocrine disruptor compounds and their spatial and seasonal distribution in Ria Formosa Lagoon (Portugal)

In spite of its outstanding ecological and touristic importance the Ria Formosa Lagoon shows signs of anthropogenic pollution. Nonetheless, until the present survey no studies had ever documented the measurement of natural and pharmaceutical estrogens (17β-estradiol, estrone, and 17α-ethynylestradiol), xenoestrogenic industrial pollutants (4-octylphenol, 4-nonylphenol, and their mono and diethoxylates and bisphenol A), phytoestrogens (formononetin, biochanin A, daidzein, genistein), and sitosterol in this area. The 17 compounds measured herein are known as endocrine disrupters (EDCs) and act over the endocrine system even in few amounts (ng L^?1–μg L^?1). Thus to conclude about the influx of EDCs in the lagoon, water samples were taken every 2 months, during 1 year (2010), in low tide at nine sites distributed along the coastline. Water samples (1 L) were preconcentrated in the Oasis HLB cartridges and cleaned in silica cartridges before their analysis by GC-MS. Data showed the ubiquitous presence of potentially hazardous amounts of estrogens (particularly of ethynylestradiol, up to 24.3 ng L^?1), nonylphenol (up to 547 ng L^?1), and sitosterol (up to 12,300 ng L^?1), mainly in summer, suggesting that the increase of the local number of inhabitants (tourists), the rise of the water temperature (up to 26 °C), and the blooming of local flora may interfere with the water quality parameters. This makes the lagoon a potential model to study. Taking into account the data, it was concluded that there are conditions for the occurrence of endocrine disruption in aquatic animals, even in areas included in the natural park of the Formosa. Besides, both the high amounts of un-ionized ammonia (up to 0.3 mg L^?1) and phosphates (up to 1.6 mg L^?1) my pose risks for local fauna and humans.     

Concentrations of lead in cosmetics commonly used in South Africa

In this study, we present evidence of lead in lip liner, oil absorbent powder, mascara, concealer, lipsticks, lip gloss, and foundation. The samples were analyzed for lead by flame atomic absorption spectrometry. The levels of lead in concealer, mascara, lip liner, and oil absorbent powder were found to be 7.4?±?1.3?µg?g^?1, 15.8?±?0.2?µg?g^?1, 29.0?±?9.2?µg?g^?1, and 17.3?±?2.9?µg?g^?1, respectively. The levels of lead in lipsticks, lip gloss, and foundation ranged from not detected to 73.1?±?5.2?µg?g^?1, 4.7 to 11.7?±?2.8?µg?g^?1, and 7.8 to 32.9?±?1.4?µg?g^?1, respectively. Thus, in the majority of samples, the concentrations of lead are higher than the USFDA maximum permissible concentration of 0.10?µg?g^?1 in candy and cosmetics. In lipsticks alone, only 25% of 40 samples were found to meet the requirement. Therefore, continuous use of cosmetic products in which lead concentration exceeds the maximum permissible level may pose a health hazard for the female population of South Africa in the long run.     

Some aspects of the chemical composition of plankton from the North-Western Mediterranean Sea

Plankton samples were collected from different parts of the Liguro-Provençal Basin during 1979 and 1980 (nearshore and open waters, respectively). Carbon, nitrogen, phosphorus, silicium, aluminium, iron, manganese, zinc, copper, cadmium and mercury concentrations have been determined in these samples. The species composition of plankton has been studies. The results indicated that species composition as well as sampling dates and location are factors of great importance when considering the chemical composition of plankton. Carbon, nitrogen and phosphorus concentrations display variations, but their ratios appear to be almost constant in the various samples. The other elements, except zinc, seem to be less concentrated in plankton from open waters than from coastal waters. The concentrations found are in good agreement with those reported in the literature by various authors.     

Organochlorine pesticides accumulation and degradation products in vegetation samples of a contaminated area in Galicia (NW Spain)

The content of 21 organochlorine pesticides were studied in vegetation samples of a highly contaminated area by isomers of hexachlorocyclohexane (HCH) located close to a former industrial area in Galicia (NW Spain). Five species of plants were collected at different points of the contaminated area and the different parts of the plants were separated in order to study differences in accumulation capabilities. Samples were extracted employing microwave energy followed by a clean-up step using solid phase extraction and finally determined by GC–ECD. The results obtained show that the most abundant pesticides are HCHs isomers, being the main isomers β-HCH and -HCH in all samples whereas δ-HCH and γ-HCH were at lower levels. Some other pesticides such as p,p′-DDT, p,p′-DDD and p,p′-DDE were also present in much lower amount in some of the samples. Several degradation products of HCH were also identified in some samples by GC–MS.     

Toxicity of hexahydro-1,3,5-trinitro-1,3,5-triazine to larval zebrafish (Danio rerio)

Hexahydro-1,3,5-trinitro-1,3,5-triazine, a cyclonitramine commonly known as RDX, is used in the production of military munitions. Contamination of soil, sediment, and ground and surface waters with RDX has been reported in different places around the world. Acute and subacute toxicities of RDX have been relatively well documented in terrestrial vertebrates, but among aquatic vertebrates the information available is limited. The objective of this study was to characterize the acute toxicity of RDX to larval zebrafish. Mortality (LC50) and incidence of vertebral column deformities (EC50) were two of the end points measured in this study. The 96-h LC50 was estimated at 22.98 and 25.64 mgl(-1) in two different tests. The estimated no-observed-effective-concentration (NOEC) values of RDX on lethality were 13.27+/-0.05 and 15.32+/-0.30 mgl(-1); and the lowest-observed-effective-concentration (LOEC) values were 16.52+/-0.05 and 19.09+/-0.23 mgl(-1) in these two tests, respectively. The 96-h EC50 for vertebral deformities on survivors from one of the acute lethality tests was estimated at 20.84 mgl(-1), with NOEC and LOEC of 9.75+/-0.34 and 12.84+/-0.34 mgl(-1), respectively. Behavioral aberrations were also noted in this acute toxicity study, including the occurrence of whirling movement and lethargic behavior. The acute effects of RDX on survival, incidence of deformities, and behavior of larval zebrafish occurred at the high end of the most frequently reported concentrations of RDX in aquatic environments. The chronic effects of RDX in aquatic vertebrates need to be determined for an adequate assessment of the ecological risk of environmental RDX.     

Evaluation of biomarkers in caged fishes and mussels to assess the quality of waters in a bay of the NW Mediterranean Sea

Specimens of sea bass (Dicentrarchus labrax) were placed in cages for 1 month in spring and autumn at different locations in the Bay of Cannes (NW Mediterranean). Biochemical markers evaluated were: ethoxyresorufin O-deethylase (EROD) and glutathione S-transferase (GST) activities in fish livers and acetylcholinesterase (AChE) activity in fish muscle. EROD and GST activities were higher in front of the outlet for the wastewater plant of Cannes and in the harbour than outside the marina. High EROD and GST activities may be induced by petrol hydrocarbons and/or polychlorinated biphenyls (PCBs). AChE was low in the muscles of the fish caged in the harbour compared with samples from the other cages. Low AChE activity could suggest the presence of organophosphorus and carbamate compounds in seawater from the harbour. Mussels (Mytilus galloprovincialis) were caged off Cannes for the same periods as the fishes. Heavy metal, metallothionein (MT) concentrations and lysosomal membrane stability were evaluated in the digestive gland of the mussels. Results show low heavy metal and MT concentrations, implying low metal concentrations in the surrounding waters. High lysosomal membrane stability revealed a good physiological status of these animals after caging. The whole set of data indicates that seawater in the Bay of Cannes appeared to be unpolluted as regards pollutants which may induce the measured biomarkers, except in restricted areas.     

Improvement of coagulation-flocculation process using anionic polyacrylamide as coagulant aid

A physicochemical treatment (coagulation-flocculation) was applied to a slaughterhouse wastewater, using anionic polyacrylamide as coagulant aid to improve the settling velocity of the flocs formed with the coagulants used: ferric sulphate, aluminium sulphate and polyaluminium chloride. The optimum speed and stirring time for the flocculation stage were ascertained along with the optimum pH and coagulant and coagulant aid doses. The speed and coagulation time were initially set according to recommendations in the literature concerning the treatment of this type of water. Chemical oxygen demand (COD), biochemical oxygen demand at 5 days (BOD5) and total suspended solids (TSS) were recorded at the beginning and end of each experiment in order to monitor the process. Once the optimal conditions had been established, several parameters were measured in order to assess the coagulation-flocculation process: particle number and size, sludge volume, nutrients (ammonia nitrogen, total Kjeldahl nitrogen, albuminoid nitrogen, orthophosphate, total phosphorus) and the residual concentration of iron and aluminium in clarified water. Anionic polyacrylamide, when added with ferric sulphate or polyaluminium chloride led to a significant increase in the settling speed.     

Tradescantia pallida cv. purpurea boom in the characterization of air pollution by accumulation of trace elements

Tradescantia pallida cv. purpurea, a plant species widely employed for ornamentation in Brazil, has been successfully used for monitoring the genotoxicity of various agents by the micronucleus assay. To amplify knowledge about its suitability as a bioindicator species, its capacity for accumulating trace elements from urban air pollution was evaluated. T. pallida was rooted using standardized soil, and the vases were distributed in two highly polluted sites of the urban area of S?o Paulo, Brazil (Cerqueira Cesar and Congonhas districts), and in one unpolluted control site situated approximately 50 km from downtown S?o Paulo (in Caucaia do Alto). Approximately six months after exposure to pollutants, adult leaves of this plant were collected monthly for 12 months. The leaves were washed with deionized water, dried, and ground for analyses. Characterization of element levels was carried out by neutron activation analysis. Powdered samples and standards were irradiated at the IEA-R1 nuclear reactor for short and long periods, and concentrations of As, Ba, Br, Ca, Ce, Cl, Cr, Co, Fe, K, La, Mn, Na, Rb, Sb, Sc, Sr, Th, and Zn were determined. Analysis of variance applied to the results indicated that samples from polluted sites present the highest concentrations of Ba, Ce, Cr, Co, Fe, La, Sb, and Sc (p < 0.05). Discriminant analysis revealed that it was possible to distinguish the two polluted areas with a precision of 97.5%, based on the amount of pollutant elements measured in the plants at each site. The results indicated the potential use of T. pallida as an accumulator plant for air pollution biomonitoring.