The proboscis extension reflex to evaluate learning and memory in honeybees (Apis mellifera): some caveats

The proboscis extension reflex (PER) is widely used in a classical conditioning (Pavlovian) context to evaluate learning and memory of a variety of insect species. The literature is particularly prodigious for honeybees (Apis mellifera) with more than a thousand publications. Imagination appears to be the only limit to the types of challenges to which researchers subject honeybees, including all the sensory modalities and a broad diversity of environmental treatments. Accordingly, some remarkable insights have been achieved using PER. However, there are several challenges to evaluating the PER literature that warrant a careful and thorough review. We assess here variation in methods that makes interpretation of studies, even those researching the same question, tenuous. We suggest that the numerous variables that might influence experimental outcomes from PER be thoroughly detailed by researchers. Moreover, the influence of individual variables on results needs to carefully evaluated, as well as among two or more variables. Our intent is to encourage investigation of the influence of numerous variables on PER results.     

Erosional Consequence of Saltcedar Control

Removal of nonnative riparian trees is accelerating to conserve water and improve habitat for native species. Widespread control of dominant species, however, can lead to unintended erosion. Helicopter herbicide application in 2003 along a 12-km reach of the Rio Puerco, New Mexico, eliminated the target invasive species saltcedar (Tamarix spp.), which dominated the floodplain, as well as the native species sandbar willow (Salix exigua Nuttall), which occurred as a fringe along the channel. Herbicide application initiated a natural experiment testing the importance of riparian vegetation for bank stability along this data-rich river. A flood three years later eroded about 680,000 m³ of sediment, increasing mean channel width of the sprayed reach by 84%. Erosion upstream and downstream from the sprayed reach during this flood was inconsequential. Sand eroded from channel banks was transported an average of 5 km downstream and deposited on the floodplain and channel bed. Although vegetation was killed across the floodplain in the sprayed reach, erosion was almost entirely confined to the channel banks. The absence of dense, flexible woody stems on the banks reduced drag on the flow, leading to high shear stress at the toe of the banks, fluvial erosion, bank undercutting, and mass failure. The potential for increased erosion must be included in consideration of phreatophyte control projects.     

Operating characteristics of a fabric filter on a peaking/cycling boiler without auxiliary preheat or reheat

A fabric filter has been on-line for one year on a coal-fired boiler that is primarily a peaking unit within the power schedule. For the first six months, Eastern Kentucky coal with 2.5% sulfur was the fuel source for 30% of the time, with low-sulfur Montana coal constituting the remaining fuel during the operation period. Bag life has been excellent with no bag failures reported to date, and the pressure drops have been low. There has never been an auxilliary heat source to preheat the fabric filter for start-up, nor to reheat the fabric filter when operating at reduced load with associated low back-end temperatures. Conclusions are that the filter cake formed does protect the bags from blinding at low load conditions, and a special acid-resistant finish applied to the glass fibers protects the bags when high-sulfur coal is burned at low temperatures. Overall, this installation provides an excellent data base for cycling service and high-sulfur coal usage with a fabric filter.     

Sustaining Economic Welfare: Estimating Changes in Total and Per Capita Wealth

Genuine saving measures the real change in0 total wealth, factoring in the depreciation of produced assets, depletion of natural resources, pollution damages, and expenditures on education. This paper presents a theoretical framework for measuring changes in wealth in total and per capita terms, establishes the link between savings measures and prospects for social welfare, and provides summary empirical estimates of the savings measures. China excepted, the majority of countries below median income are distinguished by apparent declines in total wealth per capita.     

Distribution and evolution of genetic caste determination in Pogonomyrmex seed-harvester ants

We examined the distribution and ancestral relationships of genetic caste determination (GCD) in 46 populations of the seed-harvester ants Pogonomyrmex barbatus and P. rugosus across the east-to-west range of their distributions. Using a mtDNA sequence and two nuclear markers diagnostic for GCD, we distinguished three classes of population phenotypes: those with GCD, no evidence of GCD, and mixed (both GCD and non-GCD colonies present). The GCD phenotype was geographically widespread across the range of both morphospecies, occurring in 20 of 46 sampled populations. Molecular data suggest three reproductively isolated and cryptic lineages within each morphospecies, and no present hybridization. Mapping the GCD phenotype onto a mtDNA phylogeny indicates that GCD in P. rugosus was acquired from P. barbatus, suggesting that interspecific hybridization may not be the causal agent of GCD, but may simply provide an avenue for GCD to spread from one species (or subspecies) to another. We hypothesize that the origin of GCD involved a genetic mutation with a major effect on caste determination. This mutation generates genetic conflict and results in the partitioning and maintenance of distinct allele (or gene set) combinations that confer differences in developmental caste fate. The outcome is two dependent lineages within each population; inter-lineage matings produce workers, while intra-lineage matings produce reproductives. Both lineages are needed to produce a caste-functional colony, resulting in two reproductively isolated yet interdependent lineages. Pogonomyrmex populations composed of dependent lineages provide a unique opportunity to investigate genetic variation underlying phenotypic plasticity and its impact on the evolution of social structure.     

The distribution, solid-phase speciation, and desorption/dissolution of As in waste iron-based drinking water treatment residuals

Arsenic concentrations and solid-phase speciation were assessed as a function of depth through Fe-media beds for two commercially available products (Granular Ferric Hydroxide-GFH and Bayoxide E33-E33) from pilot-scale water treatment field tests. These results were compared with data from solution (de-ionized water-DI-H2O) concentrations of As equilibrated with Fe-media in an anoxic environment at 4 degrees C. The materials had a high capacity for As (GFH media 9620 mg kg(-1) As, E33 Media 5246 mg kg(-1)). Arsenic concentrations decreased with bed depth. For E33, X-ray absorption near-edge spectroscopy results showed that As(V) was the dominant solid-phase species. For GFH, As(III) was detected and the proportion (relative to As(V)) of As(III) increased with bed depth. Arsenic concentrations in DI-H2O equilibrated with the media were low (35 microg l(-1)) over a period of 50 d. Arsenic concentrations in the equilibrated solutions also decreased with depth. Results from tests on soluble As speciation show that As in solution is in the form of As(V). Kinetic desorption experiments carried out at different pH values (3, 5, 7, 8, and 9) show that the media exhibit some acid/base neutralization capacity and tend to bind As sufficiently. Concentrations of As in the pH desorption experiments were in the same order of magnitude as the toxicity characteristic leaching procedure extractions (tens of microgl(-1)) except at low pH values. For the GFH media tested at a pH of three, As increases in solution and is mainly associated with colloidal (operationally defined as between 0.1 and 1.0 microm) iron.     

Assessment of Long-Term Trends in Fish Distributions at Multiple Scales Decreases Uncertainty Associated with Historical Datasets

Environmental Management - Monitoring long-term changes in aquatic biodiversity requires the effective use of historical data that were collected with different methods and varying levels of...     

The need to improve information on road user type in National Vital Statistics System mortality data

Objectives: Both the National Vital Statistics System (NVSS) and the Fatality Analysis Reporting System (FARS) can be used to examine motor vehicle crash (MVC) deaths. These 2 data systems operate independently, using different methods to collect and code information about the type of vehicle (e.g., car, truck, bus) and road user (e.g., occupant, motorcyclist, pedestrian) involved in an MVC. A substantial proportion of MVC deaths in NVSS are coded as “unspecified” road user, which reduces the utility of the NVSS data for describing burden and identifying prevention measures. This study aimed to describe characteristics of unspecified road user deaths in NVSS to further our understanding of how these groups may be similar to occupant road user deaths.

Methods: Using data from 1999 to 2015, we compared NVSS and FARS MVC death counts by road user type, overall and by age group, gender, and year. In addition, we examined factors associated with the categorization of an MVC death as unspecified road user such as state of residence of decedent, type of medical death investigation system, and place of death.

Results: The number of MVC occupant deaths in NVSS was smaller than that in FARS in each year and the number of unspecified road user deaths in NVSS was greater than that in FARS. The sum of the number of occupant and unspecified road user deaths in NVSS, however, was approximately equal to the number of FARS occupant deaths. Age group and gender distributions were roughly equivalent for NVSS and FARS occupants and NVSS unspecified road users. Within NVSS, the number of MVC deaths listed as unspecified road user varied across states and over time. Other categories of road users (motorcyclists, pedal cyclists, and pedestrians) were consistent when comparing NVSS and FARS.

Conclusions: Our findings suggest that the unspecified road user MVC deaths in NVSS look similar to those of MVC occupants according to selected characteristics. Additional study is needed to identify documentation and reporting challenges in individual states and over time and to identify opportunities for improvement in the coding of road user type in NVSS.