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81.
82.
E. A. Loupian A. A. Mazurov E. V. Flitman D. V. Ershov G. N. Korovin V. P. Novik N. A. Abushenko D. A. Altyntsev V. V. Koshelev S. A. Tashchilin A. V. Tatarnikov I. Csiszar A. I. Sukhinin E. I. Ponomarev S. V. Afonin V. V. Belov G. G. Matvienko T. Loboda 《Mitigation and Adaptation Strategies for Global Change》2006,11(1):113-145
83.
L. V. Mukhortova I. N. Bezkorovainaya 《Mitigation and Adaptation Strategies for Global Change》2006,11(1):191-202
The evaluation of biospheric role of the boreal forests in the accumulation of carbon is connected with the evaluation of
organic matter (OM) pool in soils. The research sites were larch forests, they are situated on Nizhne-Tungusskoe Plateau.
Larch forests of feather-moss and lichen types (110 and 380 years old) were formed on 'ochric podbur' soils. Litter stocks
are 3.5–4.5 kg m− 2 with thickness 10–25 cm. Cryomezomorphic northern taiga soils contains 38–73 t (carbon) ha− 1. Pool of fast mineralized OM has average value 38.1 t (carbon) ha− 1, including 20.5 and 6.4 t (Carbon) ha− 1 of labile compounds on surface and in the soil, and 11.2 t (carbon) ha− 1 of mobile OM. Microbial mass reaches 1.78–3.47 t (carbon) ha− 1, its proportion is 3.6–4.9% of the total OM carbon. Zoomass of feather-moss larch forest is 0.20–0.61 * 10− 2, in lichen larch forest −0.01–0.07 * 10− 2 t (carbon) ha− 1. A pool of resistant to biological decomposition and bonded to mineral soil matrix OM is 17.7 t (carbon) ha− 1 and it varies from 18.6 to 29.0 in feather-moss larch forest, and from 6.4 to 17.0 t (carbon) ha− 1 in lichen larch forest. Two-years field experiment has been performed to determine transformation rates of various plant
litter fractions and to clarify the role of soil biota in these processes. The results showed participation of all biota groups
in the decomposition of plant residues caused weight loss of larch-needles and root mortmass. Isolation of organic matter
from all-size invertebrate groups leads to some decrease of decomposition activity. 相似文献
84.
85.
Five cases of mosaicism for an isochromosome of 20q have been detected from a total of 50 000 cases analysed for prenatal diagnosis by amniocentesis. Karyotypes were designated mos 46,X_/46,X_,i(20q). In all cases, the abnormal cell line was detected in more than one primary culture, thus fulfilling the criterion for true (level III) mosaicism. Indications for prenatal diagnosis were parental anxiety (two cases), low maternal serum alpha-fetoprotein (AFP) (two cases), and high maternal serum AFP (one case). Level II ultrasounds on all five fetuses were normal, and the abnormal cell line was never detected in fetal blood and/or cord blood. All five pregnancies were continued and had normal outcomes, with birth weights ranging from 2.4 to 3.8 kg. The development of all five children has been normal, with the oldest child in the study now 4 years of age. We suggest that the abnormal cell line in each case was of extrafetal origin, and that this may be one of the more common examples of this phenomenon, occurring in approximately 1/10000 prenatal diagnoses. Mosaicism i(20q) may have been missed in the past because of the higher resolution necessary to detect this subtle change. 相似文献
86.
Counselling in connection with prenatal diagnosis (PND) is a common task for the obstetrician and the midwife. However, the decision making processes of pregnant women are not completely known, for instance, the questions of women's autonomy, the decision on how to act in the case of an abnormal test, and the partner's participation in the decision. A questionnaire and interview study was carried out among 211 women undergoing PND by amniocentesis or chorionic villus biopsy. Most women in the sample indicated that PND was completely voluntary. However, at the same time almost every woman reported that it was difficult to decline from PND when offered. Even before the visit to register at the antenatal clinic, most of the women (83 per cent) had made up their minds to have PND. At the time of the test, many of the participants (62 per cent) had decided in favour of a legal abortion if the test indicated an abnormality in the fetus. At the same time, however, the data indicate a need for reflection and ambivalence, which the medical staff have to accept. In the questionnaire most of the women stated that they and their partners had similar attitudes towards PND, but when interviewed 38 per cent of the women admitted some differences between their own attitudes and their partners'. Although some women reported considerable deliberation and ambivalence, most of them said that they would undergo PND in another pregnancy. 相似文献
87.
Paul Uvebrant Eva Björck Nils Conradi Klas-Henry Hökegård Tommy Martinsson Jan Wahlström 《黑龙江环境通报》1993,13(7):651-657
A family with two siblings, 10 and 8 years old, both with clinical and ultrastructural evidence of juvenile neuronal ceroid lipofuscinosis is described. The family was found to be informative for the restriction fragment length polymorphisms (RFLPs) detected by the probes pCJ52–95Ml (locus D16S148) and pCJ52-94Tl (locus D16S159) flanking the juvenile neuronal ceroid lipofuscinosis locus, CLN3. The parents were both heterozygous using these probes, while their two children with juvenile neuronal ceroid lipofuscinosis were both homozygous. Chorionic villi analysis showed that the fetus was heterozygous and had inherited the one allele of the mother which was not found in the two siblings. This suggested that the fetus had derived one healthy allele from the mother, the risk for a double crossing-over being less than 1 per cent. Electron microscopy showed no fingerprint inclusions in chorionic villi. The child was investigated at 6 months of age and found to be healthy, as new fingerprint inclusions were found at electron microscopy and no vacuolated lymphocytes were found in the blood smear. Due to the risk of heterogeneity, both DNA-based analysis and electron microscopy on chorionic villi are recommended for prenatal examination for juvenile neuronal ceroid lipofuscinosis. 相似文献
88.
The karyotype of cultured amniotic fluid cells obtained on the indication of advanced maternal age was shown to be a mosaic 45,X/46,X,r(?). The small size and banding pattern made it difficult to determine whether the ring was derived from and X or a Y chromosome, or even from an autosome. By using an X-centromeric probe and fluorescence in situ hybridization (FISH), we demonstrated the ring to have an X centromere. Thus, a more complete genetic counselling was possible. This confirms the usefulness of FISH in identifying and characterizing this and other chromosome rearrangements in prenatal diagnosis. 相似文献
89.
90.
With growing awareness of the problems associated with prenatal cytogenetic diagnoses after CVS, attempts have been made to provide early amniocentesis as an alternative to CVS. Since 1990, at our clinic the gestational age limit for routine diagnostic amniocentesis has been successively lowered, first to 14 and then to 13 weeks of gestation. Thus, 811 prenatal diagnoses were performed after early amniocentesis at 13 weeks (n = 217) and at 14 weeks of gestation (n = 594). No problems were encountered. Culture failure was never observed in the early samples. Using the criteria ‘number of colonies’ and ‘culture duration until harvest’, early samples taken at 14 weeks did not differ significantly from the controls after standard amniocentesis performed at 15 and 16 weeks, respectively, whereas a minority of samples taken at 13 weeks experienced some delay in culturing. However, in each group at least 85 per cent of samples led to a diagnosis fulfilling our standard criteria of a safe diagnosis (at least 20 metaphases of at least five colonies from at least one primary culture after trypsinization) within 15 days. Some differences between the different groups can be recognized: culture duration of less than 11 days tends to be increasing after standard amniocentesis, whereas long culture duration (more than 20 days) is more often associated with early amniocentesis. However, this trend is only minimal and did not result in an increased risk of missing a diagnosis. 相似文献