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71.
72.
Furtak-Cole E. Gillies J. A. Hilgendorf Z. Walker I. J. Nikolich G. 《Environmental Fluid Mechanics》2022,22(6):1399-1420
Environmental Fluid Mechanics - Wind-driven shear stresses drive saltation and dust emissions over sand dunes. However, the complex topography of coastal dunes and practical difficulties in... 相似文献
73.
Brigitte M. Manship Anthony J. Walker Lucy A. Jones Angela J. Davies 《Marine Biology》2008,153(3):473-482
Juveniles of Paragnathia formica Hesse (Isopoda; Gnathiidae) are haematophagous ectoparasites, feeding on fish blood which supplies the nutrients for their
development through growth and moulting, and the subsequent survival and reproduction of the free-living adults. Little is
known of the mechanisms of digestion in juvenile gnathiids, and biochemical studies on the digestive tract of these interesting
estuarine isopods have not been undertaken previously. Here, functionally active cathepsin-like cysteine proteinases are identified
in the digestive system of juvenile praniza (fed) and zuphea (unfed) forms. The physiological pH of the digestive tract and
the optimum proteolytic activities detected in praniza 3 homogenates using the cathepsin B/L, cathepsin B, and cathepsin H
fluorogenic substrates, N-Carbobenzoxy-Phe-Arg-4-methoxy-2-naphthylamine (Z-phe-arg-MNA), N-Carbobenzoxy-Arg-Arg-4-methoxy-2-naphthylamine (Z-arg-arg-MNA) and Arg-4-methoxy-2-naphthylamine (H-arg-MNA), respectively, are in the acidic range (pH 5.8–6.7). Inhibition profiles against Z-phe-arg-MNA and Z-arg-arg-MNA using the cathepsin B inactivator urea, and cysteine proteinase inhibitors, support the presence of cathepsin
L- and B-like enzymes. These proteolytic activities are 10–50 times higher in homogenates of praniza 3 compared with zuphea
3 forms. Histochemistry of praniza 3 sections reveals that the predominant enzyme activity towards Z-phe-arg-MNA is limited to the digestive glands during early and mid stages of digestion; later, this activity appears in
the lining of the anterior hindgut. Moreover, activity towards Z-arg-arg-MNA is generally restricted to the digestive glands, and only occasionally present in the anterior hindgut. In conclusion,
the digestive glands are the main site of cathepsin-like cysteine proteinase activities in P. formica juveniles; these enzymes appear to be important to the digestion of host fish blood enabling development through to the free-living
adult. 相似文献
74.
Kazuhiro Sonoda J. Alan. Yeakley Christopher E. Walker 《Journal of the American Water Resources Association》2001,37(6):1517-1532
ABSTRACT: We investigated spatial and temporal relationships among surface and subsurface watershed attributes and stream nutrient concentrations in urbanizing Johnson Creek watershed in northern Oregon. We sampled stream water at eight urban and five nonurban locations from March 1998 through December 1999. We sampled eight wells distributed over the two primary aquifers in the watershed. Using a Geographic Information System (GIS), percentages of landuse attributes within a radius of 30, 91, and 152 m from each sample site were quantified. We analyzed relationships between (1) nutrient concentrations and percentage cover of different landuse attributes, and (2) nutrient concentrations and underlying hydrologic units. We did not find a significant relationship between ground water chemistry and stream water chemistry. We found elevated levels of phosphorus (P) concentrations correlated with urban landuse, while higher nitrogen (N) concentrations were correlated with nonurban (primarily agricultural) landuse. We concluded that elevated levels of N in nonurban areas of Johnson Creek watershed were associated with agricultural practices. We further concluded that urban development factors such as increases in storm drains, dry wells, and impermeable surfaces may be responsible for higher input of P to the stream in urbanizing areas of the Johnson Creek watershed. 相似文献
75.
76.
The Predatory Bird Monitoring Scheme (PBMS) is a long term (>40 y), UK-wide, exposure monitoring scheme that determines the concentration of selected pesticides and pollutants in the livers and eggs of predatory birds. This paper describes how the PBMS works, and in particular highlights some of the key scientific and policy drivers for monitoring contaminants in predatory birds and describes the specific aims, scope, and methods of the PBMS. We also present previously unpublished data that illustrates how the PBMS has been used to demonstrate the success of mitigation measures in reversing chemical-mediated impacts; identify and evaluate chemical threats to species of high conservation value; and finally to inform and refine monitoring methodologies. In addition, we discuss how such schemes can also address wider conservation needs. 相似文献
77.
Green sea urchins, Strongylocentrotus droebachiensis OF Müller, collected off the coast of New Hampshire, USA, in late February 1995, were brought into the laboratory and fed
an artificial diet ad libitum, and subjected to a photoperiod advanced by 4 months. During this study, temperatures and salinities
for experimental urchins mirrored those recorded at the collection site. We examined the effects of changes in feeding regime
and photoperiod on gametogenesis and compared the experimental urchins with those from the source population. During the 7-month
period, experimental urchins showed no detectable changes in mean test height or diameter. Experimental urchins had a significantly
higher gonad index (GI) in March, April and May (18 ± 6%) compared with field urchins in March (11 ± 3%). Subsequently, experimental
urchins had a mean monthly GI of 25 to 30%, while the mean GI for field urchins was 11 to 13%. Gonial cell mitosis and gametogenesis
occurred earlier in experimental male and female urchins compared with field urchins. Stereological and histological observations
and stage–frequency data showed that the ovaries of experimental urchins were large because of the accelerated development
of nutritive phagocytes, the volume fraction (V
v) of which was 89 to 90% of the gonad, while new vitellogenic primary oocytes occupied <1% V
v. In males, stereological and histological observations and stage–frequency data suggested a mobilization of materials from
the nutritive phagocytes beginning between June and August, i.e. earlier than in females, and, by September, new gametes occupied
a V
v of 49 ± 3% of the testes. Oocyte size–frequency distributions demonstrated that most primary oocytes were <80 μm in diameter
between March and September, suggesting that cold temperatures may be needed for completion of vitellogenesis. We describe
changes in the two principal cell types in the germinal epithelium of urchin gonads and indicate how knowledge of their population
dynamics may be useful in aquaculture applications.
Received: 21 February 1997 / Accepted: 3 June 1998 相似文献
78.
79.
We used genotypic data from three highly polymorphic microsatellite loci (two autosomal and one sex-linked) to examine micro-spatial
and temporal arrangements of genetic paternity for more than 1,500 embryos housed along 12 egg-case strings of the knobbed
whelk, Busycon carica. Multiple paternity proved to be the norm in these single-dam families, with genetic contributions of several sires (at least
3.5 on average) being represented among embryos within individual egg capsules as well as along the string. Two strings were
studied in much greater detail; five and seven fathers were identified, none of which was among the several males found in
consort with the female at her time of egg-laying. Each deduced sire had fathered roughly constant proportions of embryos
along most of the string, but those proportions differed consistently among fathers. A few significant paternity shifts at
specifiable positions along an egg-case string were also observed. Although the precise physical mechanisms inside a female
whelk’s reproductive tract remain unknown, our genetic findings indicate that successive fertilization events (and/or depositions
of zygotes into egg capsules) normally occur as near-random draws from a well-but-not-perfectly blended pool of gametes (or
zygotes) stemming from stored ejaculates, perhaps in different titers, of a dam’s several mates. 相似文献
80.
William R. Walker Carol J. Haley Phyllis Bridgeman Stephen H. Goldstein 《Environmental management》1991,15(3):441-449
A literature search and survey of Virginia, USA, campgrounds with RV pump-out stations were used to determine whether boat
holding-tank deodorant chemicals would have deleterious effects on marina septic systems or package treatment plants. Laboratory
studies reported in the literature indicate that these chemical additives could affect septic system function in three ways:
(1) active ingredients in the additives can impair sewage degradation in septic tanks, causing sludge buildup and overflow
of solids into the drainfield, (2) additive chemicals might enter the drainfield and, in high enough concentrations, reduce
the drainfield's ability to degrade waste, or (3) toxic additive chemicals might migrate from the drainfield to ground or
surface water. Laboratory studies also show that some ingredients added to holding tanks interfere with functioning of activated
sludge treatment process. Experience in the field and in other laboratory studies suggests that factors such as dilution of
treated waste with untreated waste and the characteristics of the sewage to be treated can reduce the possibility of damage
to septic and activated sludge systems. The campground owners surveyed indicated that they have few problems with their septic
systems in spite of the presence of chemical additives in the RV waste. However, most of them practice good septic system
maintenance and have devised other means of ensuring that their systems function efficiently. In addition, the survey indicates
that most Virginia campgrounds get only seasonal use (as would marinas in Virginia), allowing their systems to recover between
peak seasons. 相似文献