Resource availability and the abundance of an N-based defense in Australian tropical rain forests

Plant defense theories predict that relatively resource-rich environments (those with more fertile soil) will support a greater abundance of plants with nitrogen-based chemical defense, but this has yet to be adequately tested. We tested this prediction by measuring the diversity and contribution to total biomass of cyanogenic plants (those that release hydrogen cyanide from endogenous cyanide-containing compounds) in the Australian tropical rain forest. We examined 401 species in thirty 200-m2 plots, six at each of five sites, for cyanogenesis. In upland/highland rain forest, two pairs of sites similar in rainfall and altitude, but differing in soil nutrients, were selected, as well as one site in lowland rain forest. Sites differed markedly in species composition and foliar N was positively related to soil fertility. Holding altitude constant, we did not detect significant differences in the proportion of cyanogenic species with soil fertility, nor did we consistently detect significant increases in the contribution of cyanogenic species to total biomass on higher nutrient sites. Thus we found no clear evidence that soil fertility affects the distribution and prevalence of species investing in a constitutive N-based defense at the community level.     

Spatial distribution of defense chemicals and markers and the maintenance of chemical variation

Exploring the spatial distribution of variation in plant secondary metabolites is critical for understanding the evolutionary ecology of biochemical diversity in wild organisms. In the present study, concentrations of foliar sideroxylonal, an important and highly heritable defense chemical of Eucalyptus melliodora, displayed strong, fine-scale spatial autocorrelation. The spatial patterns observed could promote associational effects on herbivore foraging decisions, which may influence the selection pressures exerted on sideroxylonal content. Multiple chemical traits have roles in certain eucalypt-herbivore interactions, and the spatial characteristics of the herbivore foraging environment are therefore determined by these different factors. We used a model of E. melliodora intake by common brushtail possums (Trichosurus vulpecula), based on the combined effects of two chemical traits, to explore this idea and found that the spatial patterns were different to those of sideroxylonal alone. Spatial genetic autocorrelation, examined using microsatellites, was strong and occurred at a fine scale, implying that restricted gene flow might allow genetic patches to respond to selection relatively independently. Local two-dimensional genetic autocorrelation, explored using a new heuristic method, was highly congruent with the pattern of local phenotypic variation observed for sideroxylonal, suggesting that the genetic variance underlying the sideroxylonal variation is similarly structured. Our results suggest that the spatial distribution of genetic and phenotypic variation could influence both the selective pressure imposed by herbivores on eucalypt defenses and the potential of populations to respond to natural selection. Spatial context should be considered in future studies of plant-herbivore interactions.     

A novel technique for the pre-concentration and extraction of inositol hexakisphosphate from soil extracts with determination by phosphorus-31 nuclear magnetic resonance

Inositol hexakisphosphate (IP6) is often the dominant form of soil organic phosphorus (P), but is rarely investigated because of the analytical difficulties encountered in its extraction, separation, and detection in environmental samples. In particular, recent advances in the study of soil organic P with 31P nuclear magnetic resonance (NMR) have been of limited use for the study of IP6, because the technique does not discriminate between IP6 and other forms of P. This was addressed by developing a novel analytical procedure using the retentive properties of gel-filtration gels for IP6, which allows the combined selective extraction and pre-concentration of IP6 from soil extracts with determination by 31P NMR. While the technique is still in the developmental stage, the results demonstrate that the gel does not interfere with 31P NMR analysis and retains IP6 to concentrations well above those required to give clear spectral signals. The technique has considerable potential for application to the study of IP6 in soil extracts and water samples and, with development, could help to answer fundamental questions regarding the dynamics of organic P in the environment.     

Distance to neighbours influences the trade-off between hiding after disturbance and defending food patches in convict cichlids (<Emphasis Type="Italic">Archocentrus nigrofasciatus</Emphasis>)

Territories are often aggregated. Because of this, distance to neighbours should influence how territory-holders balance safety from predators with the use and defence of resources. I examined the influence of distance to a neighbour on refuge use by pairs of convict cichlids (Archocentrus nigrofasciatus) faced with a conflict between hiding and defending food patches. Neighbours could reduce the rate of intrusions by strangers as a by-product of their own resource defence. This should allow fish with near neighbours to spend more time in the refuge. Neighbours could also steal from patches that are left undefended. This should lead to a reduction in use of the refuge. When one fish was confined to its refuge (so that its patch was undefended), theft by the other increased as inter-patch distance decreased. Distance between patches did not influence the rate of intrusion by non-territorial fish. When both fish defended patches, body mass influenced the effect of inter-patch distance on refuge use. Large fish rarely used the refuge, but small territory-holders spent more time in the refuge when patches were close together, as predicted. However, when one fish was dominant at both patches, distance between patches did not influence refuge use. These results suggest that, despite increased opportunity for theft, there is no realised foraging or defensive benefit to settling near neighbours that are of similar competitive ability.Communicated by J. Krause     

Integrating spatially referenced social and biophysical data to explore landholder responses to dryland salinity in Australia

Researchers attempting to integrate socio-economic data in watershed planning often draw on nationally collected census data. However, there are critical limitations to the usefulness of this type of data for decision makers operating at the watershed scale. In this paper we demonstrate the relevance of spatially referenced socio-economic data collected using mail surveys to random selections of rural landholders. The issue explored was dryland salinity management in two large watersheds in the Murray-Darling Basin of south-eastern Australia. Contrary to the assumptions underlying public policy in Australia, but consistent with the literature on farmer knowledge, comparisons of expert maps and landholder identified salinity sites suggested that landholders in these watersheds had excellent knowledge of the current extent of salinity on their property. Our research also suggested that salinity education was a sound investment by governments. At the same time, the expert maps failed to predict half of the saline affected sites identified by landholders. Accurately mapping the extent of salinity would seem a first step in addressing this nationally significant land degradation issue.     

Compartmentalization of metals within the diverse colloidal matrices comprising activated sludge microbial flocs

Activated sludge floc from a wastewater treatment system was characterized, with regard to principal structural, chemical, and microbiological components and properties, in relation to contaminant-colloid associations and settling. Multiscale analytical microscopies, in conjunction with multimethod sample preparations, were used correlatively to characterize diverse colloidal matrices within microbial floc. Transmission electron microscopy, in conjunction with energy dispersive spectroscopy (EDS), revealed specific associations of contaminant heavy metals with individual bacterial cells and with extracellular polymeric substances (EPS). Floc structure was mapped from the level of gross morphology down to the nano-scale, and flocs were described with respect to settling properties, size, shape, density, porosity, bound water content, and EPS chemical composition; gross surface properties were also measured for correlation with principal floc features. Compartmentalization results based on 171 EDS analyses and representative high-resolution images showed that nano-scale agglomerations of (i) silver (100%) and (ii) zinc (91%) were confined almost entirely to EPS matrices while (iii) Pb (100%) was confined to intracellular granules and (iv) aluminum was partitioned between EPS matrices (41%) and intracellular matrices (59%). The results suggest that engineered changes in microbial physiology and/or in macromolecular EPS composition may influence metal removal efficiencies.     

Introduction-global to local: Ecological Land Classification

Ecological Land Classification (ELC) is a scientific endeavour which attempts to organize, stratify and evaluate ecosystems (and complexes of ecosystems) for the purposes of land resource management. Since ecosystems themselves are not easily defined in practical terms, ELC is likewise not a trivial concept. Nonetheless, ELC is a prerequisite for ecosystem management and the conservation of biological diversity simply because ecosystems must be described, characterized and spatially-located before they can be managed. Regarding the current status and future direction of ELC, mainly in relation to forest management: 1) approaches to ELC construction and utilization have shifted considerably over the past 2 decades; 2) there appears to be a current consensus regarding basic approaches to ELC; 3) spatial scale is a critical variable that must be addressed by ELCs; 4) ELCs must strive to more directly address management objectives; 5) natural ecosystem functions need to be better integrated within ELC frameworks; and, 6) the need for quality, georeferenced ELC-related data will continue to grow.     

Addressing the dynamics of agri-food systems: an emerging agenda for social science research

The ‘modernist’ project that has come to dominate food and agricultural policy has failed to provide sustainable outcomes for many poor people in developing countries. Conventional agricultural science is not able to explain let alone address these concerns because it is based on a static equilibrium-centred view that provides little insight into the dynamic character of agri-food systems. This paper analyses how prevailing narratives of technological change and economic growth have come to dominate key food and agriculture policy debates. It seeks to counter these orthodox notions by emphasising that agri-food systems are embedded in complex ecological, economic and social processes, and showing how their interactions are dynamic and vulnerable to short-term shocks and long-term stresses like climate change. The paper makes the case for a deeper understanding of diverse ‘rural worlds’ and their potential pathways to sustainability through agriculture. Moreover, it argues for a normative focus on poverty reduction and concern for the distributional consequences of dynamic changes in agri-food systems, rather than aggregates and averages. Finally, it sets out an interdisciplinary research agenda on agri-food systems that focuses on dynamic system interactions in complex, risk-prone environments and explores how pathways can become more resilient and robust in an era of growing risk and uncertainty.