Enrichment and isolation of endosulfan-degrading microorganisms

Endosulfan (6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9-methano-2,3,4-benzo-dioxathiepin-3-oxide) is a cyclodiene organochlorine currently used as an insecticide all over the world and its residues are posing a serious environmental threat. This study reports the isolation and identification of enriched microorganisms, capable of degrading endosulfan. Enrichment was achieved by using the insecticide as either the sole source of carbon or sulfur in parallel studies. Two strains each of fungi (F1 and F4) and bacteria (BF2 and B4) were selected using endosulfan as a sole carbon source. A Pandoraea species (Lin-3) previously isolated in our laboratory using lindane (gamma-HCH) as a carbon source was also screened for endosulfan degradation. F1 and F4 (Fusarium ventricosum) degraded alpha-endosulfan by as much as 82.2 and 91.1% and beta-endosulfan by 78.5 and 89.9%, respectively, within 15 d of incubation. Bacterial strains B4 and Lin-3 degraded alpha-endosulfan up to 79.6 and 81.8% and beta-endosulfan up to 83.9 and 86.8%, respectively, in 15 d. Among the bacterial strains isolated by providing endosulfan as a sulfur source, B4s and F4t degraded alpha-endosulfan by as much as 70.4 and 68.5% and beta-endosulfan by 70.4 and 70.8%, respectively, after 15 d. Degradation of the insecticide occurred concomitant with bacterial growth reaching an optical density (OD600) of 0.366 and 0.322 for B4 and Lin-3, respectively. High OD600 was also noted with the other bacterial strains utilizing endosulfan as a sulfur source. Fungal and bacterial strains significantly decreased the pH of the nutrient culture media while growing on endosulfan. The results of this study suggest that these novel strains are a valuable source of potent endosulfan-degrading enzymes for use in enzymatic bioremediation.     

DYNAMICS OF SIMULATED EFFECTS OF IRRIGATION OVER DRYLAND FARMING IN SASKATCHEWAN1

ABSTRACT: Irrigation development in Saskatchewan was initiated as an instrument to bring forth regional stability through drought proofing and diversification in the agricultural sector. This development has been surrounded by controversies. Particularly, some critics questioning its economic value to the farmer. In this study, irrigation on a farm is compared as a growth alternative to the expansion of dryland farming. Under relatively conservative machine replacement policies, modest family withdrawals, government subsidized irrigation loans, and relatively favorable gross operating margins, irrigation can be a profitable undertaking in the South Saskatchewan River Basin.     

Waste materials for activated carbon preparation and its use in aqueous-phase treatment: a review

Commercial activated carbon is a preferred adsorbent for the removal of micropollutants from the aqueous phase; however, its widespread use is restricted due to high associated costs. To decrease treatment costs, attempts have been made to find inexpensive alternative activated carbon (AC) precursors, such as waste materials. Some reviews report the use of waste materials for the preparation of AC; however, these studies are restricted to either type of wastes, preparation procedures, or specific aqueous-phase applications. The present work reviews and evaluates literature dedicated both to the preparation of AC by recycling different types of waste materials and also to its application in various aqueous-phase treatments. It is clear that conventional (from agriculture and wood industry) and non-conventional (from municipal and industrial activities) wastes can be used to prepare AC, that can be applied in various aqueous treatment processes, namely to remove organic pollutants, dyes, volatile organic compounds, and heavy metals. Moreover, high surface areas can be obtained using either physical or chemical activation; however, combined treatments might enhance the surface properties of the adsorbent, therefore increasing its adsorption capacity. It is evident from the revision made that AC prepared from both conventional and non-conventional wastes might effectively compete with the commercial ones. This happens mostly when the activation procedures are optimized considering both the raw material used to produce the carbons and the contaminants to be removed.     

Relationships between soil and runoff phosphorus in small Alberta watersheds

Field-scale relationships between soil test phosphorus (STP) and flow-weighted mean concentrations (FWMCs) of dissolved reactive phosphorus (DRP) and total phosphorus (TP) in runoff are essential for modeling phosphorus losses, but are lacking. The objectives of this study were (i) to determine the relationships between soil phosphorus (STP and degree of phosphorus saturation (DPS)) and runoff phosphorus (TP and DRP) from field-sized catchments under spring snowmelt and summer rainfall conditions, and (ii) to determine whether a variety of depths and spatial representations of STP improved the prediction of phosphorus losses. Runoff was monitored from eight field-scale microwatersheds (2 to 248 ha) for 3 yr. Soil test phosphorus was determined for three layers (0 to 2.5 cm, 0 to 5 cm, and 0 to 15 cm) in spring and fall and the DPS was determined for the surface layer. Average STP (0 to 15 cm) ranged from 3 to 512 mg kg(-1), and DPS (0 to 2.5 cm) ranged from 5 to 91%. Seasonal FWMCs ranged from 0.01 to 7.4 mg L(-1) DRP and from 0.1 to 8.0 mg L(-1) TP. Strong linear relationships (r2=0.87 to 0.89) were found between the site mean STP and the FWMCs of DRP and TP. The relationships had similar extraction coefficients, intercepts, and predictive power among all three soil layers. Extraction coefficients (0.013 to 0.014) were similar to those reported for other Alberta studies, but were greater than those reported for rainfall simulation studies. The curvilinear DPS relationship showed similar predictive ability to STP. The field-scale STP relationships derived from natural conditions in this study should provide the basis for modeling phosphorus in Alberta.     

SNOW: NATURE'S RESERVOIR1

ABSTRACT: Snow, one of Nature's greatest reservoirs, supplies most of the usable water in the Western United States. Reliable predictions of the quantity and timing of the release of this water are used in making management decisions involving irrigation, stock water and municipal water supplies, hydro-power generation, recreation, navigation, and pollution control Practically oriented research is vital for the proper development and management of this resource. In southwestern Idaho, the Northwest Watershed Research Center, ARS, USDA, is conducting intensive investigations for assessing snow Volumes, snow water content, and snow-melt over a watershed. Application of these research findings will result in better development and management of the water stored as snow in Nature's reservoir.     

REAL-TIME SNOW SIMULATION MODEL FOR THE MONONGAHELA RIVER BASIN1

ABSTRACT: The Pittsburgh District, U.S. Army Corps of Engineers, is responsible for operating two multipurpose reservoirs in the 7384 square mile (19198 square kilometer) Monongahela Basin. A third reservoir, presently under construction, will soon be operating. The real-time forecasting of runoff for operational purposes requires simulation of snow accumulation and snowmelt throughout the Basin during the winter season. This article describes capabilities of SNOSIM, a model being developed for performing such simulation. The application of this model as part of a comprehensive system of water control software, and some initial simulation results are presented.     

SEDIMENT ROUTING FOR AGRICULTURAL WATERSHEDS1

ABSTRACT: A sediment routing technique was developed to route sediment yield from small watersheds through streams and valleys to the outlet of large watersheds. The technique is based on the modified universal sol loss equation and a first order decay function of travel time and particle size. Deposition is dependent upon settling velocities of sediment particles, travel time, and the amount of sediment in suspension. Sediment routing increases sediment yield prediction accuracy and allows determination of subwatershed contributions to the total sediment yield. Also, the locations and amounts of floodplain scour and deposition can be predicted. Another advantage of sediment routing is that measured sediment yield data are not required. The procedure performed satisfactorily in test routings on two Texas blackland watersheds Sediment routing will be useful in flood control evaluation, reservoir and channel design, water quality calculations, environmental impact assessment, and land-use planning.