Rock phosphate solubilizing and cellulolytic actinomycete isolates of earthworm casts

Four microbial isolates, OP2, OP3, OP6, and OP7, of earthworm casts ofPontoscolex corethrurus were found to be acid tolerant actinomycetes and efficient rock phosphate (RP) solubilizers that could grow fast on NH₄Cl-enriched or N-free carboxymethyl cellulose or glucose as sole carbon source. CMC (carboxymethyl cellulose) induced production of extracellular cellulase enzyme and the production of reducing sugar in all the isolates. RP solubilizing power was observed to be inversely related to glucose consumption. The most efficient RP solubilizer was found to consume the least glucose. Growth was faster on cellulose than on glucose media. N-free CMC induced greater glucose production than NH₄Cl-enriched CMC medium. Both CMC and glucose media were acidified by all the isolates, however, RP solubilizing power decreased with acidification. Solubilization power was greatest with isolate OP7, which also produced the greatest amount of reducing sugar per gram CMC. Both RP solubilizing power and the cellulolytic efficiency varied among isolates. A minimum of 631 μg P/0.1 g RP and a maximum of 951.4 μg P/0.1 g RP was recorded.     

Sorbents for the removal of airborne heavy metals from incinerator effluent

The effectiveness of inorganic alumino-silicate sorbents (alumina and kaolinite) to adsorb airborne lead and cadmium from the effluent stream of a simulated waste incinerator was studied. A 20 kW (68,000 BTU/h) flow reactor was used to achieve the temperature and residence times typical of a waste incinerator. Solutions containing lead or cadmium were introduced yielding airborne metals concentrations between 15 and 150 ppm. Gas samples were drawn into a particle impactor that collected the airborne particles and condensed phase metal aerosols, separating them into ten size ranges from 0.2 μm to greater than 10 μm. Metals to sorbent mass ratios between 0.03 and 1.56 were investigated. Scavenging efficiency increased as the ratio of sorbent to injected metal mass was increased. The scavenging efficiencies were as high as 76% for lead scavenged by kaolinite to as low as 14% for cadmium scavenged by alumina.     

A simple and effective approach for detecting maternal cell contamination in molecular prenatal diagnosis

The presence of maternal cells in fetal samples constitutes a serious potential source for prenatal misdiagnosis. Here we present our approach for detecting maternal cell contamination (MCC) at prenatal diagnosis for eight monogenic disorders (autosomal recessive: β-thalassaemia, sickle-cell anaemia, cystic fibrosis, prelingual deafness; autosomal dominant: achondroplasia, Huntington disease, myotonic dystrophy, neurofibromatosis type I; X-linked: spinobulbar muscular atrophy). Our aim was to apply a simple and low-cost approach, which would easily and accurately provide information on the fetal tissue MCC status. MCC testing was applied to cases of recessive inheritance where the primary mutation screening of the fetus revealed the presence of the maternal mutation, to cases concerning dominant inheritance and to cases of multiple gestation. The potential presence of maternal cells was determined by the amplification of the 3′-HVR/APO B, D1S80, THO1 and VNTRI of vWf polymorphic loci, which have previously demonstrated high heterozygosity in Caucasians. Among 135 prenatal diagnoses, 44 finally needed to be tested for MCC (32.6%). MCC was detected in four cases, where DNA was isolated directly from chorionic villi samples (CVS), and in one case with DNA isolated directly from amniotic fluid (AF). In almost 90% of cases a simple test of one polymorphic locus provided sufficient information about MCC. The choice of the appropriate locus is therefore essential, while the simultaneous screening of both parents provides the means for distinguishing non-informative sites about MCC. Copyright © 2002 John Wiley & Sons, Ltd.     

Ecophysiological indicators of the state of animal populations exposed to damaging factors

Indices of abundance and reproduction rate are considered in some groups of aquatic and terrestrial vertebrates from the zones of technogenic disasters. Upon a critical population decline caused by external destructive factors, such as emissions of acute ecotoxicants, the ecophysiological and behavioral compensatory mechanisms are activated, which provide for restoration of the total population size to the optimum within a short period of time. Environmental pollution with substances disturbing the reproductive function has the gravest consequences for animals. In this case, population size may remain fairly high, and, therefore, the effect of enhanced reproduction as a response to population decline does not take place, which eventually leads to a gradual but irreversible destruction of the population. Pathologies of reproduction should be used as a criterion for assessing the state of animals in the zones of technogenic disasters.Translated from Ekologiya, No. 1, 2005, pp. 32–38.Original Russian Text Copyright © 2005 by Shilova, Shatunovskii.     

Dandy – Walker syndrome and corpus callosum agenesis in 5p deletion

5p deletion syndrome commonly known as cri du chat is well described in affected neonates with catlike cry and hypotonia. Karyotyping will usually show a deletion of the short arm of one chromosome 5 with variable breakpoints. Only a few cases have been reported prenatally, and the fetal form of the syndrome has not been clearly individualised. We report a new case of 5p deletion syndrome diagnosed prenatally in association with Dandy–Walker syndrome and agenesis of the corpus callosum. Other brain anomalies have been reported previously, but this unusual association suggests the use of a specific probe in the investigation of these malformations. Copyright © 2005 John Wiley & Sons, Ltd.     

Paleoecology and dynamics of forest ecosystems in Central Evenkia during the past 2400 years

New data on the composition of surface assemblages of plant macroremains from soil and swamp samples have been obtained in the study of geomorphologically different localities in the middle reaches of the Nizhnyaya Tunguska River. The results of paleocarpological analysis of forest soil sections supported by relevant palynological and geochronological data are presented. Natural changes of the forest cover over the past 2400 years and quantitative characteristics of the paleoclimate during each stage are described.Translated from Ekologiya, No. 1, 2005, pp. 3–10.Original Russian Text Copyright © 2005 by Koshkarova, Koshkarov.     

A study of the growth and decay of cigarette smoke NOx in ambient air under controlled conditions

The amount of NO₂ and NO produced by the machine smoking of cigarettes was determined for 15 commercial Canadian brands. Average yield of NO was 1.44 μmoles or about 13% of the average reported for American cigarettes. Levels of NO₂ were less than 12% of NO and were probably due to the oxidation of NO. In order to assess the contribution of tobacco smoke to levels of NO in ambient air, 5 brands of cigarettes were smoked in 27 cubic meter controlled environment room. Ventilation conditions were either 2.5 or 5.0 air changes per hour (ACH) and each experiment was replicated 3 times for a total of 30 experiments. Ventilation rates of 0.3 and 1.5 ACH were also selected in a second series of experiments in which only one brand of cigarette was smoked. Least squares estimates for the effective ventilation rates were obtained in the usual manner after linearizing the decay portion of the NO time curve. In each of the experiments, the regression explained at least 95% of the variation in the levels of NO with time. Loss of NO due to factors other than ventilation appeared to be constant within experimental error and averaged 2.22 ACH. Equilibrium values for NO were grossly underestimated when results from currently accepted proecedures for smoke analysis were used in modeling the growth and decay of NO. Goodness-of-fit was improved when equilibrium values were estimated based on observed levels in ambient air. This approach may be more suitable for evaluating the potential contribution of cigarette smoke to levels of indoor air pollutants.