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Digital image analysis can be used to assist or complement visual perception. In quantifying light intensities it is superior to the human visual system and therefore is an appropriate and useful tool for research. Using microphotometry as an example the physical bases of digital image analysis are explained and the applications of this measuring technique to biochemical analysis are demonstrated. 相似文献
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Large losses of habitat could be caused by land use change that disrupts the dispersal networks used by migratory species. We assessed the relative losses of habitat for diadromous fish (i.e., those migrating between sea and freshwater) due to physical barriers, degradation of migratory passage associated with catchment land use, and site-scale land use characteristics on the West Coast, South Island, New Zealand. Fish occurrence, land use data, and river network models were analyzed in a GIS and subjected to a three-level hierarchical analysis. To identify accessible habitat not restricted by physical barriers, we used the migratory distance and maximum downstream slope encountered to identify accessible sites in least-impacted catchments and applied the results to all catchments within the study area. For two fish species, banded kokopu (Galaxias fasciatus) and koaro (G. brevipinnis), sites modeled as accessible using logistic regression in least-impacted catchments were then used to assess the impacts of catchment-scale deforestation and downstream land uses on habitat loss. Finally, sites not restricted by physical barriers or land-use-related impacts on migratory passage were used to model the effects of local land use. The models indicated that koaro and banded kokopu potentially had access to 28,000 km and 5300 km, respectively, of the 40,600 km of streams within the study area. Impacts due to intensive agricultural land use downstream in catchments affecting migratory passage were predicted to reduce the accessible habitats for koaro and banded kokopu by 55% and 70%, respectively. Local land use further reduced koaro and banded kokopu habitats to 70% and 90%, respectively, of total accessible habitat. Habitat lost through disruption of the dispersal network was disproportionately large because potentially useable habitat was rendered inaccessible. 相似文献