Anaerobic biodegradation of cellulosic material: Batch experiments and modelling based on isotopic data and focusing on aceticlastic and non-aceticlastic methanogenesis

Utilizing stable carbon isotope data to account for aceticlastic and non-aceticlastic pathways of methane generation, a model was created to describe laboratory batch anaerobic decomposition of cellulosic materials (office paper and cardboard). The total organic and inorganic carbon concentrations, methane production volume, and methane and CO₂ partial pressure values were used for the model calibration and validation. According to the fluorescent in situ hybridization observations, three groups of methanogens including strictly hydrogenotrophic methanogens, strictly aceticlastic methanogens (Methanosaeta sp.) and Methanosarcina sp., consuming both acetate and H₂/H₂CO₃ as well as acetate-oxidizing syntrophs, were considered. It was shown that temporary inhibition of aceticlastic methanogens by non-ionized volatile fatty acids or acidic pH was responsible for two-step methane production from office paper at 35 °C where during the first and second steps methane was generated mostly from H₂/H₂CO₃ and acetate, respectively. Water saturated and unsaturated cases were tested. According to the model, at the intermediate moisture (150%), much lower methane production occurred because of full-time inhibition of aceticlastic methanogens. At the lowest moisture, methane production was very low because most likely hydrolysis was seriously inhibited. Simulations showed that during cardboard and office paper biodegradation at 55 °C, non-aceticlastic syntrophic oxidation by acetate-oxidizing syntrophs and hydrogenotrophic methanogens were the dominant methanogenic pathways.     

Integration of long-term fish kill data with ambient water quality monitoring data and application to water quality management

Almost half (354) of all fish kills (805) in South Carolina, USA, between 1978 and 1988 occurred in the coastal zone. These kills were analyzed for causative, spatial, and temporal associations as a distinct data set and as one integrated with ambient water quality monitoring data. Estuarine kills as a result of natural causes accounted for 42.8% followed by man-induced (35.1%) and undetermined causes (22.1%). Although general pesticide usage was responsible for 53.9% of man-induced kills, weed control activities around resorts and municipal areas accounted for slightly more kills (20.9%) than did agricultural (19.8%) or vector control (13.2%) uses. A dramatic decline in agricultural-related kills has been observed since 1986 as the integrated pest management approach was adopted by many farmers. When taken with the few kills (12.0%) resulting from wastewaters, this suggests that these two land-use activities have been successfully managed via existing programs (IPM and NPDES, respectively) to minimize their contributions to estuarine fish kills. Ambient trend monitoring data demonstrated no coastal-wide dispersion of pesticide pollution. These data confirmed the nature of fish kills to be site-specific, near-field events most closely associated with the contiguous land-use practices and intensities. Typically, fish kill data are considered as event-specific data limited to the bounds of that event only. Our analysis has shown, however, that a long-term data set, when integrated with ambient water quality data, can assist in regulatory and resource management decisions for both short- and long-term planning and protection applications.     

Assessing ecological risk on a regional scale

Society needs a quantitative and systematic way to estimate and compare the impacts of environmental problems that affect large geographic areas. This paper presents an approach for regional risk assessment that combines regional assessment methods and landscape ecology theory with an existing framework for ecological risk assessment. Risk assessment evaluates the effects of an environmental change on a valued natural resource and interprets the significance of those effects in light of the uncertainties identified in each component of the assessment process. Unique and important issues for regional risk assessment are emphasized; these include the definition of the disturbance scenario, the assessment boundary definition, and the spatial heterogeneity of the landscape. Although the research described in this article has been funded wholly or in part by the United States Environmental Protection Agency (EPA) through Interagency Agreement Number DW89932112-01-2 to the U.S. Department of Energy, it has not been subjected to EPA review and therefore does not necessarily reflect the views of EPA and no official endorsement should be inferred.     

Biogeochemical cycling constraints on stream ecosystem recovery

In systems where production is limited by the availability of a nutrient, nutrient input to and recycling within the system is related to the resilience, or speed of recovery, of a system to its steady state following a disturbance. In particular, it is shown that the return timeT _s of the system to steady state, or the inverse of the resilience, is approximately equal to the mean turnover time of the limiting nutrient in the system. From this relationship, it is possible to understand and predict how various properties of food webs and their environments affect resilience. These properties include nutrient input rate, loss rate, size of the detritus compartment, and trophic structure. The effects of these properties on resilience are described by using simple mathematical models. To test model predictions, experimental studies of the response of periphyton-dominated stream ecosystems to disturbance are being conducted on a set of laboratory streams in which nutrient inputs and grazing intensity are regulated at different levels. In streams without snail grazers (low-grazed streams), 90% recirculation of stream water to reduce nutrient inputs resulted in longer turnover times (T _r ) of phosphorus within the stream compared with once-through flow. However, in streams with snail grazers (high-grazed streams), there were no differences in phosphorus turnover time between once-through and partially recirculated treatments. Results on the rate of recovery of periphyton from a flood/scour disturbance to each stream partially support the model prediction of a positive relationship between ecosystem return time (T _s ) and nutrient turnover time (T _r ) within the streams.     

APPLICATION OF A SEDIMENT ROUTING MODEL TO A MIDDLE EUROPEAN WATERSHED1

ABSTRACT: A simulation model that computes sediment yield due to sheet and rill erosion at the outlet of a large watershed requires daily precipitation and the soil, topographic, and vegetative characteristics of the watershed. An important problem, particularly in a large watershed, is the transport of sediment produced in the sub-watersheds to the outlet of the whole watershed. This problem is approached mathematically by a sediment routing model that is used as a component of the total model.     

Integrating race and adult psychosocial development

The biographical interview method was used to study the lives of 10 black male entrepreneurs. The findings of this investigation supported Levinson's theory of developmental periods. The concept of evolving life structure was found to be vibrant and viable. Because of the impact of racial dynamics and racism, on the men's lives, two new developmental tasks of adult psychosocial development are proposed: (1) to form an individual racial identity that both acknowledges and frees the individual of their own racism and prejudices, and (2) to form an individual self-concept dedicated to the eradication of racial discrimination and racial prejudice from society.     

Long-term depletion of calcium and other nutrients in eastern US forests

Both harvest removal and leaching losses can deplete nutrient capital in forests, but their combined long-term effects have not been assessed previously. We estimated changes in total soil and biomass N, Ca, K, Mg, and P over 120 years from published data for a spruce-fir site in Maine, two northern hardwood sites in New Hampshire, central hardwood sites in Connecticut and Tennessee, and a loblolly pine site in Tennessee. For N, atmospheric inputs counterbalance the outputs, and there is little long-term change on most sites. For K, Mg, and P, the total pool may decrease by 2%–10% in 120 years depending on site and harvest intensity. For Ca, net leaching loss is 4–16 kg/ha/yr in mature forests, and whole-tree harvest removes 200–1100 kg/ha. Such leaching loss and harvest removal could reduce total soil and biomass Ca by 20%–60% in only 120 years. We estimated unmeasured Ca inputs from rock breakdown, root-zone deepening, and dry deposition; these should not be expected to make up the Ca deficit. Acid precipitation may be the cause of current high leaching of Ca. Although Ca deficiency does not generally occur now in acid forest soils, it seems likely if anthropogenic leaching and intensive harvest removal continue.     

EFFECTS OF PLACER MINING DISCHARGE ON HEALTH AND FOOD OF ARCTIC GRAYLING1

ABSTRACT: During the summers of 1982, 1983, and 1985, we assessed the effects of placer mining sedimentation on Arctic grayling, Thymallus arcticus, in the headwaters of Birch Creek, northeast of Fairbanks, Alaska. We compared differences between two streams (one that was undisturbed and one with mining activity upstream) near the confluence. Studies of caged fish demonstrated that, if grayling could not escape from streams carrying mining sediments, they would either die at high rates (sac fry) or suffer gill damage, starvation, and slowed maturation (age-O fingerlings and age-2 juveniles). Indirect effects of sedimentation, through loss of summer habitat for feeding and reproduction, may more severely affect grayling populations than the direct effects of sedimentation on the health and survival of individual fish.