Evaluations of combined zebrafish (Danio rerio) embryo and marine phytoplankton (Diacronema lutheri) toxicity of dissolved organic contaminants in the Ythan catchment,Scotland, UK

A wide variety of organic contaminants including pesticides, polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) have previously been detected in surface waters in the river Ythan catchment, North East Scotland UK. While the concentrations detected were below Water Framework Directive Environmental Quality Standards (WFD-EQSs) environmental exposures to the diverse mixtures of contaminants, known and unknown, may pose chronic and/or sublethal effects to non target organisms. The present study assessed the embryo and algal toxicity potential of freely dissolved organic contaminants from the Ythan catchment using silicone rubber passive sampling devices (SR-PSDs) and miniaturised bioassay techniques. Zebrafish (Danio rerio) embryos and marine phytoplankton species (Diacronema lutheri) were exposed to extracts from SR-PSDs deployed at different locations along the river Ythan and an undeployed procedural blank. Statistically significant developmental and algal toxicities were measured in all tests of extracts from deployed samples compared with the procedural blanks. This indicates environmental exposure to, and the combined toxicity potential of, freely dissolved organic contaminants in the catchment. The present and previous studies in the Ythan catchment, coupling SR-PSDs and bioassay techniques, have both helped to understand the interactions and combined effects of dissolved organic contaminants in the catchment. They have further revealed the need for improvement in the techniques currently used to assess environmental impact.     

Lethal and sublethal effects of simvastatin,irgarol, and PBDE-47 on the estuarine fish,Fundulus heteroclitus

This study investigated the effects of simvastatin, a lipid-regulating drug; irgarol, an antifouling biocide; and PBDE-47, a brominated flame retardant, on the estuarine fish, Fundulus heteroclitus. Sublethal effects (changes in glutathione (GSH), lipid peroxidation (LPx), acetylcholinesterase (AChE), and cholesterol (CHL) levels) and lethal effects (survival) were determined after individual exposure to the three compounds. There were no significant differences in GSH or CHL levels in fish exposed to any of the test compounds. LPx levels significantly decreased with increasing irgarol concentrations. AChE levels were significantly lower in fish exposed to simvastatin at the 1.25 mg/L concentration and significantly higher at the PBDE-47 concentration of 0.0125 mg/L. The LC50 values were 2.68, 3.22, and > 0.1 mg/L for simvastatin, irgarol and PBDE-47, respectively.     

Development of a low-cost air sensor package and indoor air quality monitoring in a California middle school: Detection of a distant wildfire

A low-cost air sensor package was used to monitor indoor air quality (IAQ) in a classroom at the Albany Middle School in the San Francisco Bay Area of California. A rapid increase in carbon dioxide (CO₂) was observed in the classroom as soon as it is occupied. When the classroom is unoccupied, the CO₂ levels decay slowly toward the outdoor background level. All high CO₂ concentrations observed inside the classroom, above the outdoor background, was due to exhaling of the occupants. The CO₂ concentrations generally exceed the recommended level of 1000 ppb towards the end of the school day. The exceedances and slow decay may suggest that the ventilation rate in this school is not sufficient. The particulate level in the classroom was low until a distant wildfire advected large amount of particulate matter to the San Francisco Bay Area. Very high (10–15 times compared to the background) particle numbers (per m³ of particles with diameter >0.3 µm) were observed in the classroom during the wildfire. These particles were relatively small (0.3–1.0 µm) and the filters (MERV 8) of the ventilation system were unable to filter them out. Therefore, the measurements made by low-cost particle counters can inform the school administrators of adverse IAQ during future wildfire (or other combustion) events. The particle number was independent of the occupation before and during the wildfire suggesting that all observed particles were infiltrated into the classroom from outside. Consistent with previous studies, no appreciable increase in the local ambient CO₂ background was observed during this distant wildfire event.

Implications: Low-cost air sensors are effective in monitoring indoor air quality in classrooms. The CO₂ levels in classrooms are mainly generated indoors due to exhalation of occupants. Concentration of CO₂ generally exceed the recommended level of 1000 ppb towards the end of the school day. In contrast, the particulate matter mostly comes from outdoors and small particles penetrate though the filters normally used at schools. Distant wildfires do not increase the local CO₂ background appreciably, but significantly increase the particulate matter concentrations both indoors and outdoors. Further investigations are needed to assure that ventilation rates in classrooms are sufficiently health protective.     

Measurement of Indoor Air Emissions from Dry-Process Photocopy Machines

Abstract

Presently, no standard test method exists to evaluate the various emissions from office equipment (e.g., ozone, volatile organic compounds, inorganic gases, and particulates) so it is difficult to compare data from different studies.1 As a result, the authors are developing a standardized guidance document for measuring indoor air emissions from office equipment. The ultimate goal is to apply the test method to better understand emissions from office equipment and to develop lower emitting machines. This paper provides background information on indoor air emissions from office equipment with an emphasis on dry-process photocopy machines. The test method is described in detail, along with the results of a study to evaluate the test method using four dry-process photocopy machines.

The results from this study indicate that the test method provides acceptable performance for characterizing emissions; that it can adequately identify differences in emissions between machines both in compounds emitted and their emission rates; and that it is capable of measuring both intra- and inter-machine variability in emissions. Challenges and complications were encountered in developing and implementing the test method. These included heat generation, which can cause large increases in chamber temperature; finite paper supplies for photocopy machines, which limit test duration; varying power requirements that may require changes in chamber electrical supply; and remote starting of the machines, which is necessary to maintain chamber integrity.

Results show that dry-process photocopy machines can produce emissions of ozone and volatile organic compounds that can potentially have a significant impact on indoor air quality. For the four machines tested in this study, the compounds with the highest emission rates overall were ethylbenzene (28,000 µg/hour), m,p-xylenes (29,000 µg/hour), o-xylene (17,000 µg/hour), 2-ethyl-lhexanol (14,000 µg/hour), and styrene (12,000 fig/hour). Although many of the same compounds tended to be detected in emissions from each of the four photocopiers, the relative contribution of individual compounds varied considerably between machines, with differences greater than an order of magnitude for some compounds.     

Acute effects of removing large fish from a near-pristine coral reef

Large animals are severely depleted in many ecosystems, yet we are only beginning to understand the ecological implications of their loss. To empirically measure the short-term effects of removing large animals from an ocean ecosystem, we used exclosures to remove large fish from a near-pristine coral reef at Palmyra Atoll, Central Pacific Ocean. We identified a range of effects that followed from the removal of these large fish. These effects were revealed within weeks of their removal. Removing large fish (1) altered the behavior of prey fish; (2) reduced rates of herbivory on certain species of reef algae; (3) had both direct positive (reduced mortality of coral recruits) and indirect negative (through reduced grazing pressure on competitive algae) impacts on recruiting corals; and (4) tended to decrease abundances of small mobile benthic invertebrates. Results of this kind help advance our understanding of the ecological importance of large animals in ecosystems.     

Predicted uranium and radon concentrations in New Hampshire (USA) groundwater—Using Multi Order Hydrologic Position as predictors

Two radioactive elements, uranium (U) and radon (Rn), which are of potential concern in New Hampshire (NH) groundwater, are investigated. Exceedance probability maps are tools to highlight locations where the concentrations of undesirable substances in the groundwater may be elevated. Two forms of statistical analysis are used to create exceedance probability maps for U and Rn in NH groundwater. The first, Boosted Regression Tree (BRT), was selected for estimating U exceedance values. It computes exceedance values directly using the Bernoulli distribution function. The second method of statistical analysis used for Rn to determine exceedance probabilities is ordinary least squares (OLS) regression. In the process of determining exceedance probabilities for U and Rn, the utility of a new dataset is investigated. That new predictor dataset is the Multi-Order Hydrologic Position (MOHP) dataset. MOHP raster datasets have been produced nationally for the conterminous United States at a 30-m resolution. The concept behind MOHP is that, for any given point on the earth's surface, there is the potential for a longer groundwater flow path as one goes deeper beneath the land surface. MOHP predictors were tested in both models. Three MOHP predictors were found useful in the BRT model and two in the OLS model. MOHP data were found useful as predictors along with other site characteristics in predicting U and Rn exceedance probabilities in New Hampshire groundwater.     

The cost of being an omnivore: mandible wear from plant feeding in a true bug

Evolutionary and ecological transitions from carnivorous to omnivorous feeding may be constrained by the ability of the animal to cope with disparate types of foods, even if preadaptations for such behaviour exist. The omnivorous true bug, Dicyphus hesperus (Hemiptera: Miridae) requires both animals (small, soft-bodied insects) and plants in its diet and obtains the majority of its dietary and metabolic water from plant feeding. Serrations on the lateral margins of the mandibular stylets wear with age, and this wear is exacerbated when the insects feed on plants compared to those provided free water and no plants. D. hesperus that feed on plants attack fewer prey but consumed similar amounts of prey tissue compared to individuals that were provided free water. Although others have shown mandible wear for plant-chewing animals we show for the first time that plant feeding can impose similar wear on plant-piercing animals as well.     

Ecological consequences of forest elephant declines for Afrotropical forests

Poaching is rapidly extirpating African forest elephants (Loxodonta cyclotis) from most of their historical range, leaving vast areas of elephant‐free tropical forest. Elephants are ecological engineers that create and maintain forest habitat; thus, their loss will have large consequences for the composition and structure of Afrotropical forests. Through a comprehensive literature review, we evaluated the roles of forest elephants in seed dispersal, nutrient recycling, and herbivory and physical damage to predict the cascading ecological effects of their population declines. Loss of seed dispersal by elephants will favor tree species dispersed abiotically and by smaller dispersal agents, and tree species composition will depend on the downstream effects of changes in elephant nutrient cycling and browsing. Loss of trampling and herbivory of seedlings and saplings will result in high tree density with release from browsing pressures. Diminished seed dispersal by elephants and high stem density are likely to reduce the recruitment of large trees and thus increase homogeneity of forest structure and decrease carbon stocks. The loss of ecological services by forest elephants likely means Central African forests will be more like Neotropical forests, from which megafauna were extirpated thousands of years ago. Without intervention, as much as 96% of Central African forests will have modified species composition and structure as elephants are compressed into remaining protected areas. Stopping elephant poaching is an urgent first step to mitigating these effects, but long‐term conservation will require land‐use planning that incorporates elephant habitat into forested landscapes that are being rapidly transformed by industrial agriculture and logging.     

Motorcycle helmet visor–related facial injury and its potential mechanism of injury: Evidence-based case study

Objectives: We encountered an unusual facial laceration wound in relation to motorcycle helmet visor use during our clinical practice. We aimed to assess the prevalence of this unusual facial injury among motorcyclists who sustained facial injuries in selected hospitals and to determine the possible mechanism involved.

Methods: We used our prospective cross-sectional substudy involving injured motorcyclists presenting at major trauma hospitals in Southern Klang Valley, Malaysia, between March 2010 and March 2011. of 391 subjects with facial injuries, 2 male motorcyclists sustained this laceration. The wounds were assessed and we believed that each was associated with the helmet visor. One of the visors was collected and the edge was inspected using scanning electron microscopy (SEM).

Results: The prevalence of this unusual injury was 0.51% (95% confidence interval, 0.002–0.012) among motorcyclists who sustained facial injuries. Both cases were involved in a head-on collision with their colliding partners and their helmets were intact throughout the crash. The visor in case 1 was intact, but the visor in case 2 was broken. SEM analysis showed that the visor in case 1 had a potential cutting surface.

We postulated that with helmet rotation in the forward and downward position and with some degree of visor bending or with a dislodged visor, the sharp-edged visor could potentially severely lacerate the face.

Conclusion: This injury affects facial aesthetics and early referral to the facial surgery team is advocated. Documentation of the mechanism of injury, the patient’s helmet and visor is obligatory, so that this information can be delivered to the regional road safety authority for preventive measures.