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41.
Dietrich Mebs Georg Petschenka Werner Pogoda Stefan W. Toennes Jens Amendt Barbara K. Zajac Susanne Dobler 《Chemoecology》2014,24(4):159-164
The blowfly Lucilia bufonivora shows high host specificity for toads despite the host’s toxic skin secretion, which consists mainly of bufadienolides. These toxins are effective blockers of the Na+, K+-ATPase, an enzyme that is essential for many physiological processes in animals. Whereas common toad (Bufo bufo) toxins were identified in the larvae of the fly, few toxins were found in the pupae and empty puparia as trace amounts, while adult flies were entirely free of these toxic compounds. Similar results were obtained when larvae of generalist necrophagous blowflies (L. sericata, Calliphora vicina) fed on toad carcasses. Analysis of the Na+, K+-ATPase gene revealed no amino acid substitution at positions known to mediate resistance to bufadienolides in other systems. Alternative mechanisms of resistance such as efficient excretion of the compounds may enable the flies to use this poisonous food source. 相似文献
42.
Quantification of copepod gut content by differential length amplification quantitative PCR (dla-qPCR) 总被引:1,自引:0,他引:1
Christofer Troedsson Paolo Simonelli Verena Nägele Jens C. Nejstgaard Marc E. Frischer 《Marine Biology》2009,156(3):253-259
Quantification of feeding rates and selectivity of zooplankton is vital for understanding the mechanisms structuring marine
ecosystems. However, methodological limitations have made many of these studies difficult. Recently, molecular based methods
have demonstrated that DNA from prey species can be used to identify zooplankton gut contents, and further, quantitative gut
content estimates by quantitative PCR (qPCR) assays targeted to the 18S rRNA gene have been used to estimate feeding rates
in appendicularians and copepods. However, while standard single primer based qPCR assays were quantitative for the filter
feeding appendicularian Oikopleura dioica, feeding rates were consistently underestimated in the copepod Calanus finmarchicus. In this study, we test the hypothesis that prey DNA is rapidly digested after ingestion by copepods and describe a qPCR-based
assay, differential length amplification qPCR (dla-qPCR), to account for DNA digestion. The assay utilizes multiple primer
sets that amplify different sized fragments of the prey 18S rRNA gene and, based on the differential amplification of these
fragments, the degree of digestion is estimated and corrected for. Application of this approach to C. finmarchicus fed Rhodomonas marina significantly improved quantitative feeding estimates compared to standard qPCR. The development of dla-qPCR represents a
significant advancement towards a quantitative method for assessing in situ copepod feeding rates without involving cultivation-based
manipulation. 相似文献
43.
Axel Strauß Katrin Y. Solmsdorff Roger Pech Jens Jacob 《Behavioral ecology and sociobiology》2008,62(10):1551-1558
Predators can strongly influence the microhabitat use and foraging behaviour of prey. In a large-scale replicated field experiment
in East Gippsland, Australia, we tested the effects of reduced alien red fox (Vulpes vulpes) and alien wild dog (Canis lupus familiaris) abundance (treatment) on native bush rat (Rattus fuscipes) behaviour. Bush rats are exposed to two main guilds of predators, namely mammalian carnivores and birds of prey. Tracking
rat movements using the spool-and-line technique revealed that, in treatment sites, rats used ground cover, which provides
shelter from predators, less often than at unmanipulated fox and wild dog abundance (non-treatment sites). In treatment sites,
rats more frequently moved on logs where they would have been exposed to hunting foxes and dogs than in non-treatment sites.
Furthermore, in treatments, rats showed a preference for understorey but not in non-treatments. Hence, bush rats adapted their
behaviour to removal of alien terrestrial predators. Giving-up densities (GUDs) indicated no treatment effects on the marginal
feeding rate of bush rats. Interestingly, GUDs were higher in open patches than in sheltered patches, suggesting higher perceived
predation risk of bush rats during foraging at low versus high cover. The lack of treatment effects on GUDs but the clear
response of bush rats to cover may be explained by the impact of predators other than foxes and wild dogs. 相似文献
44.
Risk-based regulation assumes that risk assessment can evaluate risks against policy objectives. However, policy goals are often ambiguous and require risk assessors to interpret them for use in risk assessment. This risk assessment-policy gap stems partly from normative and imprecise policy language but is rooted more fundamentally in society's uncertain expectations for the environment. Until this uncertainty is resolved, the democratic and regulatory effectiveness of risk regulation will be undermined by ad hoc policy decisions abdicated to risk assessors. 相似文献
45.
Boy Jens Strey Simone Schönenberg Regine Strey Robert Weber-Santos Oscarlina Nendel Claas Klingler Michael Schumann Charlotte Hartberger Korbinian Guggenberger Georg 《Regional Environmental Change》2018,18(1):63-75
Regional Environmental Change - Soil carbon stocks of 29 plots along a transect through tropical Brazil showed only minor soil carbon losses after land use shift, although replacement of... 相似文献
46.
For modern organizations, shared leadership becomes increasingly important. Knowledge on shared leadership may be limited, as past research often relies on cross-sectional data or student samples, and most studies neglect the multilevel nature of shared leadership. Our research model includes transformational leadership, trust, and organizational support as predictors of shared leadership. Furthermore, we analyze the influence of shared leadership on team performance and team creativity. In total, 160 teams with 697 employees participated in our field study. Data collection took place at three time points. To test our hypotheses, we used multilevel modeling with a Bayesian estimator. We found relationships of transformational leadership and trust with shared leadership at the team level and of transformational leadership, trust, and organizational support with shared leadership at the individual level. Furthermore, shared leadership fully mediated the effect of the three input factors on team performance and team creativity. This study contributes to the understanding of the antecedents and outcomes of shared leadership. Furthermore, the dynamic development of team processes based on an input–mediator–output model is explored. On the basis of the results, organizations can increase shared leadership behavior by focusing on transformational leadership and trust building. 相似文献
47.
Peter Leinweber Ulrich Bathmann Uwe Buczko Caroline Douhaire Bettina Eichler-Löbermann Emmanuel Frossard Felix Ekardt Helen Jarvie Inga Krämer Christian Kabbe Bernd Lennartz Per-Erik Mellander Günther Nausch Hisao Ohtake Jens Tränckner 《Ambio》2018,47(1):3-19
This special issue of Ambio compiles a series of contributions made at the 8th International Phosphorus Workshop (IPW8), held in September 2016 in Rostock, Germany. The introducing overview article summarizes major published scientific findings in the time period from IPW7 (2015) until recently, including presentations from IPW8. The P issue was subdivided into four themes along the logical sequence of P utilization in production, environmental, and societal systems: (1) Sufficiency and efficiency of P utilization, especially in animal husbandry and crop production; (2) P recycling: technologies and product applications; (3) P fluxes and cycling in the environment; and (4) P governance. The latter two themes had separate sessions for the first time in the International Phosphorus Workshops series; thus, this overview presents a scene-setting rather than an overview of the latest research for these themes. In summary, this paper details new findings in agricultural and environmental P research, which indicate reduced P inputs, improved management options, and provide translations into governance options for a more sustainable P use. 相似文献
48.
Heinz Rüdel Josef Müller Jens Nowak Mathias Ricking Roland Klein Matthias Kotthoff 《Environmental science and pollution research international》2017,24(22):18048-18062
The brominated flame retardant hexabromocyclododecane (HBCD) was monitored in fish and sediment (from one lake) or suspended particulate matter (SPM; from five rivers) at European freshwater sites to study the effects of reduction measures implemented by HBCD producers and users in recent years. Bream (Abramis brama) were sampled annually between 2007 and 2013 in the rivers Götaälv/SE, Rhône/FR, Western Scheldt/NL, Mersey/UK, and Tees/UK and in Lake Belau/DE. Sediment/SPM was taken every second year between 2008 and 2014. HBCD was analyzed by LC/MS/MS allowing the determination of the alpha-, beta-, and gamma-diastereomers. For most sites, a decrease in ∑HBCD was observed in fish (e.g., in the Rhône and Western Scheldt by about 80 and 60%, respectively, with significantly decreasing trends, p < 0.01). In the Rhône, HBCD also decreased in SPM. At the sampling site in the Tees which was impacted by a former HBCD point source, fish HBCD levels decreased only after a major flood event in 2013. While fish data indicate a decline in environmental HBCD concentrations at most sites with diffuse emissions, SPM data were less conclusive. The European environmental quality standard for HBCD in fish of 167 μg kg?1 wet weight was met by all fish samples in 2013. 相似文献
49.
Blood serum concentrations of perfluorinated compounds in men from Greenlandic Inuit and European populations 总被引:3,自引:0,他引:3
Lindh CH Rylander L Toft G Axmon A Rignell-Hydbom A Giwercman A Pedersen HS Góalczyk K Ludwicki JK Zvyezday V Vermeulen R Lenters V Heederik D Bonde JP Jönsson BA 《Chemosphere》2012,88(11):1269-1275
Perfluorinated compounds (PFCs), such as perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), are used in large quantities. They are persistent and found in measurable levels in human serum around the world. They have been associated with developmental, hepatic, and carcinogenic effects in animal studies. The aim of the present study was to describe levels of PFCs in serum among Inuits from Greenland and inhabitants from Warsaw, Poland and Kharkiv, Ukraine. Furthermore, the aim was to define social- and lifestyle related determinants of exposure for these compounds. Serum levels of seven PFCs were analyzed by liquid chromatography/tandem mass spectrometry (LC/MS/MS). The concentrations of PFOS and PFOA were the highest of all PFCs in all three populations with a total amount of almost 90% of the PFCs. The mean levels of PFOS and PFOA were in the Greenlandic Inuits 52 and 4.8 ng mL(-1), in Poland 19 and 5.2 ng mL(-1), and in Ukraine 8.1 and 1.9 ng mL(-1), respectively. Thus, levels of PFCs in the serum of Inuits on Greenland were among the highest described in a general population whereas the levels in Poland were similar to other industrialized countries. The exposure in Ukraine was rather low. In the Greenlandic Inuit population, intake of seafood, tea, age and area of living were significant determinants of PFOS concentrations and explained about 22% of the variation. For the other populations no strong determinants were found. 相似文献
50.
Rosenbom AE Therrien R Refsgaard JC Jensen KH Ernstsen V Klint KE 《Journal of contaminant hydrology》2009,104(1-4):137-152
This study numerically investigates the influence of initial water content and rain intensities on the preferential migration of two fluorescent tracers, Acid Yellow 7 (AY7) and Sulforhodamine B (SB), through variably-saturated fractured clayey till. The simulations are based on the numerical model HydroGeoSphere, which solves 3D variably-saturated flow and solute transport in discretely-fractured porous media. Using detailed knowledge of the matrix, fracture, and biopore properties, the numerical model is calibrated and validated against experimental high-resolution tracer images/data collected under dry and wet soil conditions and for three different rain events. The model could reproduce reasonably well the observed preferential migration of AY7 and SB through the fractured till, although it did not capture the exact depth of migration and the negligible impact of the dead-end biopores in a near-saturated matrix. A sensitivity analysis suggests fast flow mechanisms and dynamic surface coating in the biopores, and the presence of a plough pan in the till. 相似文献