Waste recombinant DNA: Effectiveness of thermo-treatment to manage potential gene pollution

Heating at 100 °C for 5–10 min is a common method for treating wastewater containing recombinant DNA in many bio-laboratories in China. In this experiment, plasmid pET-28b was used to investigate decay efficiency of waste recombinant DNA during thermo-treatment. The results showed that the decay half-life of the plasmid was 2.7–4.0 min during the thermo-treatment, and even heating for 30 min the plasmids still retained some transforming activity. Low pH promoted the decay of recombinant DNA, but NaCl, bovine serum albumin and EDTA, which existed in the most wastewater from bio-laboratories, protected DNA from degradation. Thus, the decay half-life of plasmid DNA may be longer than 2.7–4.0 min practically. These results suggest that the effectiveness of heating at 100 °C for treating waste recombinant DNA is low and a gene pollution risk remains when those thermo-treated recombinant DNAs are discharged into the environment. Therefore other simple and effective methods should be developed.     

Study on mechanics of crowd jam based on the cusp-catastrophe model

Some characteristics of crowd jam like the phenomena of discontinuous jumping in reality are hard to be explained by the equations governing pedestrians. The catastrophe theory can explain these characteristics. A cusp-catastrophe model is developed to analyze the mechanics of crowd jam by drawing graphs for a cusp-catastrophe model of crowd, the bifurcation set and the projection of catastrophe model. Meanwhile, the critical density and the critical velocity are derived. It is concluded that the cusp model is a more efficient predictor than the linear model or the equations governing pedestrians and is reasonably realistic for dense crowd flow scenarios.     

故障树分析法在高炉炉前灼伤事故中的应用

应用故障树分析法能系统地分析高炉炉前灼伤的原因，在故障村图上，因果关系明确，并能找出事故发生的主要原因，从而制定措施，以有效地预防事故的发生。     

An ensemble Kalman filter for atmospheric data assimilation: Application to wind tunnel data

In the previous work (Zheng et al., 2007, Zheng et al., 2009), a data assimilation method, based on ensemble Kalman filter, has been applied to a Monte Carlo Dispersion Model (MCDM). The results were encouraging when the method was tested by the twin experiment and a short-range field experiment. In this technical note, the measured data collected in a wind tunnel experiment have been assimilated into the Monte Carlo dispersion model. The uncertain parameters in the dispersion model, including source term, release height, turbulence intensity and wind direction have been considered. The 3D parameters, i.e. the turbulence intensity and wind direction, have been perturbed by 3D random fields. In order to find the factors which may influence the assimilation results, eight tests with different specifications were carried out. Two strategies of constructing the 3D perturbation field of wind direction were proposed, and the result shows that the two level strategy performs better than the one level strategy. It is also found that proper standard deviation and the correlation radius of the perturbation field play an important role for the data assimilation results.     

朱集矿13-1煤瓦斯参数规律研究

为了采取合理的瓦斯抽采技术,实现矿井安全高效开采,对朱集矿13-1煤瓦斯基本参数进行了现场和实验室测定,得出了瓦斯含量、瓦斯压力、放散初速度,透气性系数、坚固性系数等参数,并对测定结果进行了理论分析,得出了这些参数的基本规律.结果表明:随着开采深度的增加,在一定深度范围内,其瓦斯压力、瓦斯含量呈线性增长,瓦斯压力与瓦斯含量都比较大；煤层钻孔瓦斯衰减系数较大,该煤层的透气性系数较小,D、K值小于其临界值,可判断出该煤层为难抽煤层且有突出危险性.     

吸油烟机风量检测中可靠性分析

根据GB/T 17713-2011的要求,对外排式吸油烟机空气性能实验装置的板孔的设计进行了研究,结合板孔系数表,提出了与实际工作点最接近的计算方法,以设计的孔板测量外排式吸油烟机的风量。并根据实际的检测实例,找出了实际影响因素,提高了风量检测的可靠性。     

农业文化遗产地有机生产转换期农产品价格补偿测算——以云南省红河县哈尼梯田稻作系统为例

高效农业技术广泛使用提高了农业生产效率,促进了粮食产量增长,但同时也带来环境问题、食品安全问题和传统农业文化逐渐消失等负面效应。哈尼梯田作为全球重要农业文化遗产(GIAHS),具有生态价值、农业生产价值和景观价值等多重价值,保护哈尼梯田农业系统意义重大。通过有机生产提高稻谷价格的方式,推动农民继续种植水稻,可以达到保护哈尼梯田农业系统的目的。然而,从非有机到有机生产有一个转换期,这期间稻谷无法以有机产品的价格出售,所以,政府须给予一定的价格补偿才可保证农民利益和生产的持续性。论文采用问卷和访谈等调查方法,获取哈尼梯田地区农户有机转换期种植投入产出状况和劳动力外出务工收入状况,以及现代规模生产方式下的投入产出状况,通过分析对比和核算,得到结果如下:1哈尼梯田地区有机转换期水稻种植直接投入高于现代水稻种植方式,总投入成本中劳动力成本占比最大,而单产低于现代水稻种植方式;2哈尼梯田地区的青壮年劳动力偏向于在城市生活和务工,外出务工收入明显高于常规农业收入,使农业机会成本较高;3有机转换期的稻谷价格补偿至少2.84元/kg才可保证有机转换期农民收入稳定,从而达到保护哈尼梯田景观的目的。     

工业VOCs经济手段和工程技术减排对比性分析

挥发性有机物(VOCs)污染已经同二氧化硫、氮氧化物和颗粒物等成为目前我国重点城市群的主要大气污染物.工业领域是挥发性有机物的主要排放部门,工程技术减排是重要的控制措施之一."十二五"期间,我国计划投资400亿元建设重点行业挥发性有机物污染治理项目,年减排量计划为60.5万t·a-1.技术减排被提到了一个新的高度,表明了国家对工程技术减排的重视.本研究采用宏观经济学可计算的一般均衡模型,对工程技术减排与经济手段(环境税)进行政策模拟,进行比对性分析,探讨了两种减排方法的优劣.研究认为,当前经济条件下,相同减排量下,考虑宏观经济损失,环境税减排的成本远高于技术减排.结合目前我国实际情况,为鼓励工程技术减排,建议政府给予企业约为7 500元·t-1的财政补贴.     

微波辐照褐煤半焦脱除低浓度NO的研究

选用褐煤半焦在微波辐照条件下脱除低浓度NO,研究了微波功率、空速、停留时间和O2浓度对脱硝率的影响。结果表明,在微波加热条件下,功率越大,褐煤半焦温度越高,脱硝效果越好。在微波功率250 W时(半焦中心温度400℃左右),脱硝率达到92%,空速越小即停留时间越长,越有利于NO的去除,O2的加入有利于促进NO的还原。褐煤半焦的脱硝性能仅次于活性炭,远高于焦炭,证实了选取的褐煤半焦是一种性能优越的微波脱硝材料。     

水生生物水质基准研究中轮虫、水螅、涡虫类受试生物的筛选

轮虫、水螅、涡虫是水生生态系统的重要生物类群。因其对水体污染较敏感,所以对水生生物基准研究有重要意义。依据我国生物区系资料及毒性数据丰度,筛选出8种代表性本土轮虫、水螅、涡虫类生物。参照美国水生生物基准技术指南,搜集、筛选了这8种代表性生物的急性毒性数据,通过数据分析,筛选出对各物种毒性最大的污染物,主要包括重金属、农药、有机锡化物、表面活性剂、吡啶胺类杀菌剂。分析污染物的物种敏感度分布,依据累积概率对代表性生物的物种敏感性进行分类,结果为:萼花臂尾轮虫(Brachionus calyciflorus)、绿水螅(Hydra viridissima)、普通水螅(Hydra vulgaris)对重金属铜的累积概率为6.5%、8.5%和10.4%,普通水螅(Hydra vulgaris)对重金属汞的累积概率为6.3%;龟甲轮虫(Keratella cochlearis)和四齿腔轮虫(Lecane quadridentata)对五氯酚钠的累积概率为5.1%和7.6%;褐水螅(Hydra oligactis)和绿水螅(Hydra viridissima)对三丁基氧化锡的累积概率为6.9%和13.8%,萼花臂尾轮虫(Brachionus calyciflorus)对氟啶胺的累积概率为6.7%,日本三角涡虫(Dugesia japonica)对四氯化碳、十二烷基苯磺酸钠的累积概率分别为6.7%和7.1%。上述结果表明:萼花臂尾轮虫、绿水螅对重金属铜敏感;普通水螅对重金属铜和汞敏感;龟甲轮虫和四齿腔轮虫对农药敏感;褐水螅和绿水螅对有机锡化物敏感;萼花臂尾轮虫对吡啶胺类杀菌剂敏感;日本三角涡虫对四氯化碳、表面活性剂敏感。这7种代表性生物可作为相关污染物的水生生物基准受试物种。