Evaluation of factors influencing soluble microbial product in submerged MBR through hybrid ASM model

In this study, a mathematical model was established to predict the formation of the soluble microbial product (SMP) in a submerged membrane bioreactor. The developed model was calibrated under the reference condition. Simulation results were in good agreement with the measured results under the reference condition. The calibrated model was then used in the scenario studies to evaluate the effect of three chosen operating parameters: hydraulic retention time (HRT), dissolved oxygen concentration, and sludge retention time (SRT). Simulation results revealed that the SMP dominated the soluble organic substances in the supernatant. The scenario studies also revealed that the HRT can be decreased to 1 h without deteriorating the effluent quality; dissolved oxygen concentration in the reactor can be kept at 2–3 mg/L to maintain the effluent quality, reduce the content of SMP, and minimize operating costs; the optimal SRT can be controlled to 10–15 d to achieve complete nitrification process, less membrane fouling potential, and acceptable organic removal efficiency.     

Olfactory host finding, intermediate memory and its potential ecological adaptation in Nasonia vitripennis

Associative learning of host-associated chemical cues was studied in Nasonia vitripennis, a parasitoid of fly pupae in nests of hole-nesting birds. When females encountered a fly pupa and performed one sequence of host recognition behaviour including drilling the ovipositor into the host in the presence of the artificial odour furfurylheptanoate (FFH), they were afterwards arrested by FFH in olfactometer experiments. The response vanished after 4 days and could be blocked after 3 days by feeding wasps with ethacrynic acid prior and after the training. This indicates the formation of an intermediate form of memory by one host experience in N. vitripennis. Interestingly, the trained wasps avoided odours that were not present during the host encounter, although naive wasps did not react to these odours. This unique behaviour probably causes wasps to focus during host searching on those chemical cues they have experienced in the host environment. Studies in nests of hole-nesting birds revealed that about 30% of all nests contained only one fly pupa, and laboratory studies showed that N. vitripennis females are able to parasitise around 100 fly pupae in their life. It is discussed that under these conditions, the formation of a non-permanent intermediate memory for host-associated odours after one host encounter is adaptive to avoid costs involved with formation and maintenance of memory for misleading cues. The demonstration of associative olfactory learning in N. vitripennis, the first parasitoid species with sequenced genome, opens the gate to study molecular mechanisms of memory formation and its ecological adaptation in parasitoids.     

Levels and determinants of beta(1-->3)-glucans and fungal extracellular polysaccharides in house dust of (pre-)school children in three European countries

BACKGROUND: Mold growth is believed to be one causative factor underlying the association between dampness in buildings and increased respiratory morbidity. Measurements of beta(1-->3)-glucans and fungal extracellular polysaccharides (EPS) are used as markers of mold exposure in field studies. Little is known about their levels and determinants in homes. OBJECTIVE: To study levels and determinants of beta(1-->3)-glucan and EPS levels in mattress and living room floor dust in three European countries. METHODS: Mattress and living room floor dust was collected in the homes of 1065 German, Dutch, and Swedish (pre-)school children. All samples were analyzed for beta(1-->3)-glucans and EPS in one central laboratory. Determinants were assessed by questionnaire. RESULTS: Amounts of dust, EPS and beta(1-->3)-glucan levels differed between countries. Amounts of dust, beta(1-->3)-glucan and EPS levels for mattresses were only weakly correlated with those for living room floors. Floor dust beta(1-->3)-glucan loads, EPS loads and EPS concentrations were strongly correlated with the amount of dust sampled, which is largely determined by the type of floor that was sampled (carpeted floors had 5-20 higher amounts of dust). None of the other determinants was consistently and statistically significantly associated with amounts of dust, beta(1-->3)-glucan and EPS concentrations on floors and mattresses. CONCLUSION: Mattress dust and floor dust are two different measures of exposure to the investigated mold components. Living room floor beta(1-->3)-glucan and EPS loads and EPS concentrations are largely determined by the type of floor sampled. Differences between countries can only partly be explained by the determinants studied.     

Coastal livelihood and physical infrastructure in Bangladesh after cyclone Aila

This paper aimed at to explore the consequences of cyclone victims due to unavailability of infrastructural supports and to prop up the recognition that the infrastructure has a vital role to play in societies’ resilience during catastrophic situation. The paper begins with a review of the science regarding climate change impact and cyclone disaster in Bangladesh. It emphasizes the consequences of cyclone Aila in a selected coastal community in the remote coastal area. A field survey was conducted by authors during March-August 2009. Eight available infrastructures were selected for this analysis. Uniformity of distribution (R), demand index (Di) and degree of demand (DD) of the selected infrastructures were calculated by using nearest neighbourhood methods of analysis. Results show that based on its specific planning standards none of the selected infrastructure can support 50% of the total population. Accordingly, it was observed that 76% respondent could not reach in safer place due to rush of water intrusion and also because of the inundation of road-network. The nearness to the available cyclone shelter, and place of taking shelter during cyclone is positively correlated (r = 0.38; p < 0.001). However, the poor people had less opportunity to take shelter in cyclone shelters, although none of the respondents groups whose monthly income is above 75 USD stands without any infrastructural support. Such important observation may hint the influence of local elites on the local disaster mitigation planning practice in Bangladesh. Almost 90% of the respondents claimed that they had no access to enter the available cyclone shelter. Furthermore, the damaged infrastructures added more hindrances during post disaster activities and also increased the sufferings of the victims. If there were adequate cyclone shelters or rehabilitation centre, the affected people could take shelter and continue other works temporarily. Results drawn from this research will be useful for local and national level planners, as well as international donors for future disaster mitigation planning in the studied area and the methodology can be applied in similar countries and geographical territories.     

Genotoxic effects of Ag2S and CdS nanoparticles in blue mussel (Mytilus edulis) haemocytes

The use of functionalised metal sulphide nanoparticles (NPs) for nanoremediation and biomedical application is rapidly increasing, which could lead to significant inputs into the marine environment. The potential impact of some NPs on marine organisms is still poorly understood. In the present paper the genotoxic potential of Ag₂S and CdS NPs on Mytilus edulis haemocytes was assessed. MPEG-SH (thiol-terminated methyl polyethylene glycol), was used as capping agent to avoid NPs agglomeration. TEM analysis showed that the Ag₂S NPs size was 13±7 nm, whereas CdS quantum dots had an average diameter of 4±1 nm. DNA integrity was evaluated by Comet assay following exposure to increasing concentration series (0.01–10 mg/L). Both silver and cadmium NPs showed genotoxic effects at the highest dose. MPEG-SH was also found to exert a weak genotoxic activity, suggesting that at least part of the genotoxic potential of functionalised NPs on mussel cells might be attributable to the capping agent. These results confirm the genotoxic potential of Ag₂S NPs for mussel cells and demonstrated, for the first time, that CdS NPs is genotoxic in a marine organism.     

Short term effects of copper, sulfadiazine and difloxacin on the anaerobic digestion of pig manure at low organic loading rates

Antibiotics of inorganic and organic origin in pig manure can inhibit the anaerobic process in biogas plants. The influence of three frequently used antibiotics, copper dosed as CuSO₄, sulfadiazine (SDZ), and difloxacin (DIF), on the anaerobic digestion process of pig manure was studied in semi-continuous experiments. Biogas production recovered after every Cu dosage up to a sum of 12.94 g Cu kg⁻¹ organic dry matter (ODM), probably due to Cu precipitation following the formation of sulphide from sulphate. Complete inhibition was found at the very high Cu concentration of 19.40 g Cu kg⁻¹ ODM. Inhibitory effect of SDZ and DIF was observed at concentrations as high as 2.70 g kg⁻¹ ODM and 0.54 g kg⁻¹ ODM, respectively. It seems very unlikely that the antibiotics tested would inhibit the anaerobic process in a full-scale biogas plant.     

Spatial surrogates for the disaggregation of CORINAIR emission inventories

CORINAIR atmospheric emission inventories are frequently used input data for air quality models with a domain situated in Europe. In CORINAIR emission inventories, sources are broken down over 11 major source categories. This paper presents spatial surrogates for the disaggregation of CORINAIR atmospheric emission inventories for input of air pollutants and particulate matter to grid or polygon based air quality model domains inside Europe. The basis for the disaggregation model was the CLC2000 land cover data to which statistical weights were added. Weights were population census data for residential emissions, employment statistics for agricultural and industrial area emissions, livestock statistics for ammonia emissions and annual aircraft movements for emissions realized by air transport. Additional road and off-road network information was used to disaggregate emissions realized by traffic. A comparison of top down produced emission estimates with spatially resolved national emission data for The Netherlands and the United Kingdom gave confidence in the present spatial surrogates as a tool for the top down production of atmospheric emission maps. Explained variance at a spatial resolution of 5 km was >70% for CO, NMVOC and NO_x, >60% for PM10 and almost 50% for SO₂.     

Challenges of diverse knowledge systems in landscape analysis of the Murray–Darling Basin,Australia

Regional Environmental Change - Geographic information systems are a means to develop a common framework for the integration of a range of perspectives into natural resources management decisions....     

Nestmate recognition in social wasps: manipulation of hydrocarbon profiles induces aggression in the European hornet

The influence of individual cuticular hydrocarbons on nestmate recognition in the European hornet, Vespa crabro L., was investigated. We observed the behavioural response of workers towards differently treated dead conspecifics in a bioassay. Dummies were extracted with dichloromethane and extracts were spiked with microgram amounts of synthetic hydrocarbons naturally occurring on the cuticle of V. crabro. These modified extracts were reapplied to extracted workers that were subsequently tested in the bioassay. Non-spiked nestmate dummies (negative control) and untreated non-nestmate dummies (positive control) were tested in control experiments. The addition of only heneicosane or a mixture of heneicosane, tricosane, and (Z)-9-tricosene to the extracts led to a significant increase of agonistic behaviour in workers leaving the nest for foraging flights. Returning workers reacted much less aggressively than those leaving. This is one of the first behavioural proofs that manipulation of cuticular hydrocarbon profiles can be perceived by a social insect species. The results support the hypothesis that colony-specific cuticular hydrocarbon profiles are involved in the phenomenon of nestmate recognition among social insects.     

瑞典森林中不同地区的林下植被情况与氮沉积的关系

在557块针叶林分中进行调查,以图了解欧洲越桔(Vacciniummyrtillus)、越桔(V.vitis-idaea)和曲芒发草(Deschampsiaflexuosa)与氮沉积率的相互联系.欧洲越桔在高氮地区比在低氮地区发生频度、丰富度都较少并且易感染叶部病原(Valdensiaheterodoxa),越桔的发生与氮沉积的增加呈明显的负相关,曲芒发草却没有这种趋势.在高氮沉积地区,欧洲越桔在优势种为欧洲赤松的林分中比在挪威云杉为优势种的林分中更为最普遍.在氮沉积较低水平的地区不是这种情形.调查的情况与增施氮素实验的结果一致,氮沉积对植被显示了巨大的效应.数据表明即使是较低的氮沉积也可能减少针叶林分林下植被最优势种的丰富度.