Etude des variations du poids sec et de la constitution chimique élémentaire de Tisbe holothuriae (Copepoda: Harpacticoïda) élevé sur différents régimes artificiels

Dry weight and body composition of C, H and N were investigated in male and female Tisbe holothuriae Humes fed 7 artificial compound diets and a living algal diet (Nannochloris sp.) over the whole biological cycle. No correlation was found between the respective proportion of C, H and N in the food and in the copepod body. Food quality had a significant effect on dry weight. Carbon content varied between 35.35 and 42.32% of body dry weight in females, and between 25.10 and 26.84% in males. Nitrogen values were 9.11 to 10.00% and 6.79 to 6.99%, respectively, varying with different diets. The C:N ratio varied between 3.74 and 4.28 in females and between 3.70 and 3.87 in males. Some diets had a significant effect on this ratio. In some experiments, three different temperatures were tested using the same diet. Body dry weight was inversely correlated with temperature, but no clear relation appeared between C:N and temperature. C:N variations were directly related to C concentration and seemed independent of N concentration. It is suggested that C variations correspond to different levels in lipid content under different trophic conditions. The relation between elementary chemical composition and fecundity in T. holothuriae fed different diets is discussed.     

Arsenic management in shale oil upgrading

Although the timing remains uncertain, the production of synthetic crude from oil shale seems to be a likely response to diminishing petroleum reserves. Although not unique to shale oil, the presence of arsenic, ranging in concentration from a few to over 100 parts per million, poses a special concern.Arsenic is typically removed from the shale oil by a hydro-processing technique and is concentrated on a catalyst as thermally stable metal arsenides and metal arsenous sulfides. Because this arsenic is still slightly soluble in water, making the spent catalyst a hazardous material, passivation and extraction techniques were investigated to make the catalyst safe for disposal. Passivation techniques were not successful. While extraction procedures were capable of removing over 75% of the arsenic, treated material may still be acceptable.     

Behavioral responses of larvae of the crab Rhithropanopeus harrisii (Brachyura: Xanthidae) during diel vertical migration

Larval Rhithropanopeus harrisii (Gould) show nocturnal vertical migration. Larval behavioral responses to different rates of increase and decrease in light intensity were measured in an apparatus with a natural angular light distribution. A central objective was to establish whether phototaxis actually participates in vertical migration. At sunset the level of light adaptation controlled the readiness of the larvae to migrate, while an ascent was initiated by a preductable relative decrease in intensity (e.g. 4.0x10^-3s^-1). Rates of relative decrease around sunset would evoke continuous upward swimming. Gravity was the orienting cue and there was no change in swimming speed during the ascent. At sunrise, the larval descent was initiated by exposure to an absolute light intensity of about 0.23 log unit above the lower visual threshold. Light served as an orienting cue, as larvae descended by a negative phototaxis. Thus, phototaxis is not a laboratory artifact and does participate in vertical migration. A consideration of behavioral responses of other crustacean zooplankton indicates there is considerable variation in the initiating and directing cues for vertical movements. The variety of behavioral responses of R. harrisii suggests that a synthesis of hypotheses about migration may provide the proper basis for explaining the mechanisms underlying diel vertical migration.     

New development in the MOCNESS,an apparatus for sampling zooplankton and micronekton

Four variants of the Multiple Opening/Closing Net and Environmental Sensing System (MOCNESS) have been constructed to sample a broad size spectrum of oceanic animals from microzooplankton to micronekton. The systems differ in mouth opening dimensions (ranging from 1/4 to 20 m²), the number of nets carried (from 5 to 20), and the mesh size of the netting (from 64 m to 3.0 mm). A new electronics package enables an operator to send commands down a single conductor, armored cable to open/close the nets and provides 12-bit resolution for the environmental (temperature, depth, conductivity) and net operation data (flow, net-frame angle, net-bar release), which are transmitted up the cable to the deck unit at 2-s intervals. A microcomputer system, interfaced to the deck unit, calculates salinity, volume filtered by a net, net trajectory velocity, and vertical velocity. The data are printed out and stored on disc, and profiles of temperature and salinity versus depth are plotted during the course of the tow. Analysis of the relationship between the geometry of the MOCNESS under tow and the past and present methods used to estimate the water filtered by a net revealed that significant bias is introduced when the ascent or descent angle of a net is disregarded. The bias is a function of the ratio of vertical velocity to net trajectory velocity and results in an underestimate of volume filtered while shooting a net and an overestimate while hauling.     

Parental investment decision rules and the Concorde fallacy

Summary Investment theory states that animals should base their parental investment decisions on expected benefits, and not on whether or not past investment will be wasted. Otherwise, they would comnit the Concorde fallacy. If reproduction has a cost, however, then past investment and expected benefits are necessarily confounded. Assuming a cost of reproduction, animals will be selected to maximize their remaining lifetime reproductive success, subject to a tradeoff between present and future reproduction (Williams' principle). We extend Williams' principle and develop an experimental design that would allow past investment and expected benefits to be varied independently. This design illustrates the importance of the value of the brood relative to the value of future reproduction.     

Occurrence and significance of nitrogen transport in the brown alga Laminaria digitata

Nitrogen transport in a Laminaria digitata (Huds.) Lamour population growing at Arbroath, Scotland has been investigated (during the period 3 June 1981 to 3 July 1981), using ¹⁵N as tracer. NO ₃ ^- was assimilated both by the blade meristem and by the mature blade. NO ₃ ^- uptake by the blade meristem alone was insufficient to supply the nitrogen demand for growth. This additional demand was met by nitrogen transport from the mature blade to the meristem. It was estimated that 70% of the nitrogen demand of the meristematic region was supplied by nitrogen transport from the mature blade. Although transport occurred, the size of the endogenous pool of stored nitrogen in the mature blade did not change, the quantity of nitrogen transported being equivalent to the amount of NO ₃ ^- assimilated during the experimental period. The possible co-transport of nitrogen and carbon is discussed.     

Mechanisms of photoadaptation in three strains of the symbiotic dinoflagellate Symbiodinium microadriaticum

Mechanisms of photoadaptation of photosynthesis have been studied in three strains of the symbiotic dinoflagellate Symbiodinium microadriaticum. Algal strains isolated from the clam Tridacna maxima, the sea anemone Aiptasia pulchella, and the scleractinian coral Montipora verrucosa were maintained in the defined medium ASP-8A, and were grown at irradiances ranging from 22 to 248 μE m^-2 s^-1 on a 14 h:10 h (light:dark) photoperiod at 26°C. All algal cultures were analysed during log-phase of growth. At all light levels, rates of cell division and photosynthesis were determined, as were cell volumes, pigmentation (including chlorophyll a, chlorophyll c ₂, peridinin, β-carotene and xanthophylls), and carbon and nitrogen content. Low light-induced changes in pigmentation were evident to varying degrees in all three algal strains, although alterations in the photosynthesis-irradiance relations were distinctly different in each strain. The algae from T. maxima show the least photoadaptive capability, and seem to photoadapt by changing photosynthetic unit (PSU) size. Algae from A. pulchella appear to adapt by changing PSU number, while algae from M. verrucosa appear to photoadapt by changes in the activities of CO₂-fixing enzymes or electron transport systems. These are the first observations that demonstrate functional differences in different strains of S. microadriaticum. The adaptive capabilities of the algae appear to correlate well with the ecological distribution of their respective hosts. The study was made from July 1981 through December 1982.     

Prodigiosin metabolites of a marine Pseudomonas species

The major pigment of a marine micro-organism was identified as prodigiosin on the basis of analytical and spectral data. Two minor pigments were tentatively assigned structures, from thin-layer chromatography (TLC) and mass spectral data, as prodigiosin homologs with 3-n-hexyl and 3-n-heptyl side-chains. The micro-organism was classified as a new Pseudomonas species on the basis of its morphological and biochemical characteristics. It was named Pseudomonas magnesiorubra (ATCC No. 21856).     

Effect of zinc on the settlement of the oyster Crassostrea gigas

The influence of zinc upon the pattern and success of settlement was examined in the oyster Crassostrea gigas. Late larvae were more tolerant of zinc than embryos. A delay in settlement was recorded in treatments containing zinc as low as 125 μg/l, and numbers of larvae settling was reduced in the presence of zinc. Larvae subjected to zinc immediately prior to settlement showed evidence of slowing in behavioural development. Those larvae which settled in the presence of zinc, however, when ongrown in clean water were as viable as controls. Zinc at concentrations of 250 and 500 μg/l suppressed spat growth, but recovery was rapid upon subsequent on-growing in clean water conditions.