Evaluation of the infectivity, gene and antigenicity persistence of rotaviruses by free chlorine disinfection

The effects of free chlorine disinfection of tap water and wastewater effluents on the infectivity, gene integrity and surface antigens of rotaviruses were evaluated by a bench-scale chlorine disinfection experiments. Plaque assays, integrated cell culture-quantitative RT-PCR (ICC-RT-qPCR), RT-qPCR, and enzyme-linked immunosorbent assays (ELISA), respectively, were used to assess the influence of the disinfectant on virus infectivity as well as genetic and antigenic integrity of simian rotavirus SA11 as a surrogate for human rotaviruses. The ICC-RT-qPCR was able to detect rotaviruses survival from chlorine disinfection at chlorine dose up to 20 mg/L (60 min contact), which suggested a required chlorine dose of 5 folds (from 1 to 5 mg/L) higher than that indicated by the plaque assay to achieve 1.8 log₁₀ reductions in tap water with 60 min exposing. The VP7 gene was more resistant than the infectivity and existed at chlorine dose up to 20 mg/L (60 min contact), while the antigencity was undetectable with chlorine dose more than 5 mg/L (60 min contact). The water quality also impacted the inactivation efficiencies, and rotaviruses have a relatively higher resistant in secondary effluents than in the tap water under the same chlorine disinfection treatments. This study indicated that rotaviruses have a higher infectivity, gene and antigencity resistance to chlorine than that previously indicated by plaque assay only, which seemed to underestimate the resistance of rotaviruses to chlorine and the risk of rotaviruses in environments. Present results also suggested that re-evaluation of resistance of other waterborne viruses after disinfections by more sensitive infectivity detection method (such as ICC-RT-qPCR) may be necessary, to determine the adequate disinfectant doses required for the inactivation of waterborne viruses.     

Estimating carbon supply curves from afforestation of agricultural land in the Northeastern U.S.

The Regional Greenhouse Gas Initiative for the northeastern states of the U.S. allows for terrestrial carbon (C) sequestration offsets generated by afforestation activities only. This paper estimates the maximum potential quantity and associated costs of increasing the storage of carbon by afforestation of existing agricultural land in the 11 states of the Northeast United States. The focus of the work was to describe location, the quantity, and at what cost it would be economically attractive to shift agricultural production to afforestation to increase carbon storage in the region. Widely available data sets were used to (1) identify spatially-explicit areas for lower costs carbon offsets and (2) estimate carbon supply curves related to afforestation of agricultural land over three time periods (10, 20, and 40 years). Carbon accumulation and total carbon offset project costs were estimated at a county scale and combined to identify expected costs per ton of carbon dioxide equivalents (CO₂e). Large variation in estimated costs per ton of CO₂e are driven by varying carbon accumulation potentials and opportunity costs of taking land out of agricultural production, as well as the duration of the project activity. Results show that the lowest cost carbon offset projects will be in certain counties of Maine, Vermont, and New York. Pasture land, with lower opportunity costs, generally presents the opportunity for lower cost carbon offset projects relative to cropland. This analysis estimates that afforestation of pasture land in the northeast will not become economically attractive until the price rises above 10 per metric tonne (MT) CO < sub > 2 < /sub > e and that up to 583 million MT could be economically sequestered if the price were to rise to10 per metric tonne (MT) CO₂e and that up to 583 million MT could be economically sequestered if the price were to rise to 50 per MT CO₂e, based on a 40-year project life. With regard to cropland in the northeast, afforestation does not become economically advantageous for land owners until the price rises above $40 per MT CO₂e. It is estimated that up to 487,000 MT could be sequestered from cropland if the price were to rise to $40 per MT CO₂e. It is estimated that up to 487,000 MT could be sequestered from cropland if the price were to rise to 50 per MT CO₂e, based on a 40-year project life.     

Effects of long-term nutrient addition on meso-micro soil arthropod communities in a Stipa baicalensis grassland北大核心CSCD

To analyze the effect of nutrient addition on small- and medium-sized soil arthropod communities in a Stipa Baikal meadow grassland, a nitrogen (N) and phosphorus (P) addition experiment was designed in the Stipa Baikal meadow grassland at Ewenki Banner, Hulunbeier City, Inner Mongolia, China in 2010. Changes in the structure and diversity of soil arthropod communities and their relationship with environmental factors were studied. Soil arthropod samples were collected during the forage greening (May), growth (August), and yellowing (end of September) periods in 2019. The results showed that: (1) compared to the control sample, N-added and NP-added samples had higher number of soil arthropods by 1.38 and 1.15 times, respectively, and an increase of 28.57% and 21.43% in the number of soil arthropod groups. The numbers of individual as well as groups of soil arthropods tended to increase with P addition; however, this response did not reach a significant level. (2) The water and heat conditions during the vegetation growth period in the test area were more suitable for the development of soil fauna communities. (3) The redundancy analysis (RDA) results further indicated that the community composition of soil fauna is mainly regulated by pH and plant evenness, and soil pH is particularly important in mediating changes in small- and medium-sized soil fauna communities. This study suggests that long-term nutrient addition has a positive impact on small- and medium-sized soil arthropod communities by changing plant evenness and soil pH, and the contribution of soil pH is greater than that of plant evenness. © 2022 Authors. All rights reserved.     

Construction of an ecosystem service value evaluation system for the Longquan Mountain Urban Forest Park in Chengdu City Based on the Analytic Hierarchy Process北大核心CSCD

As a special ecosystem, an urban forest park, which is different from a regular “forest park”, is one of the key elements involved in improving the urban ecological environment and promoting the sustainable development of a city. The “urban forest park” can also significantly improve living conditions by acting as a greenbelt. The Chengdu Longquan Mountain Urban Forest Park is a rare mountain urban forest park located in the center of the city. This “urban forest park” not only provides more ecological products and maintains ecological security but also meets the growing needs of people for food, environmental quality, and spirituality. To comprehensively assess the ecological value of the Chengdu Longquan Mountain Urban Forest Park, this study used the literature analogy and questionnaire survey methods to establish a targeted ecosystem service assessment system in this study area. In addition, combined with the hierarchical analysis method, the indicator system was tested, and the specific evaluation index was determined. In the Longquan Mountain Urban Forest Park Ecosystem Service Value Index System, three first-level indicators, such as ecological material products (with a weight of 0.412 6), ecological regulation (0.327 5), and dwelling culture (0.259 9); eight secondary indicators, such as product production (0.206 3), resource supply (0.206 3), and biodiversity (0.194 4); and twenty-three tertiary indicators, such as air negative oxygen ion (0.154 7), habitat quality (0.095 9), and ecological health benefit (0.075 5), were identified. From the weights of the first and second indicators, it was clear that the supply of ecological material products is the main service function in the Longquan Mountain Urban Forest Park ecosystem. Moreover, from the weights of the third indicator, the public’s cognition and concept of the ecosystem service value of the “urban forest park” gradually shifted from the supply service of products and resources to cultural services. In summary, the ecosystem service value assessment system designed for the Longquan Mountain Urban Forest Park in this study has certain feasibility and extensibility that lays a theoretical foundation for the scientific assessment of ecological value and ecological value realization mechanism for other “forest parks” worldwide. © 2022 Authors. All rights reserved.     

Identification and expression of the COI1 gene family in Camellia sinensis and its expression in oolong tea processing北大核心CSCD

茉莉酸受体蛋白COI1(coronatine insensitive 1)是茉莉酸信号转导途径的重要组成部分,为鉴定分析茶树茉莉酸受体COI1基因家族,预测其潜在的分子功能,了解茉莉酸受体COI1基因在乌龙茶加工中应答胁迫的分子机制,利用生物信息学方法对茶树茉莉酸受体COI1进行家族成员鉴定,氨基酸序列、结构域、基因结构、进化分析以及启动子顺式元件分析,结合实时荧光定量分析CsCOI1基因在乌龙茶加工中的表达.结果显示,茶树茉莉酸受体CsCOI1家族有两个成员,均含有F-box和富亮氨酸重复序列(LRRs)两个结构域;单子叶、双子叶的COI1蛋白各聚一支,且与蜜柑进化关系较近;茶树COI1基因家族两个成员均含有3个内含子,启动子顺势元件主要有胁迫响应元件、激素响应元件以及光响应元件;转录组数据说明茶树CsCOI1基因具有较强的组织表达差异性.实时荧光定量分析表明,CsCOI1a基因在室内萎凋后表达显著上调,且15 min、30 min日光萎凋后CsCOI1b基因的表达水平显著上调,同时茉莉酸含量发生显著变化.本研究推测CsCOI1基因可能通过茉莉酸信号转导途径参与乌龙茶加工中萎凋的胁迫响应过程,该结果可为乌龙茶加工品质调控奠定基础.(图8表2参30)     

Progress of the scientific expedition and research on farmland ecosystem and food security in Qinghai-Tibet Plateau北大核心CSCD

介绍第二次青藏高原综合科学考察研究任务三生态系统与生态安全专题三“农田生态系统与粮食安全”科学考察背景、总体思路、研究内容、研究目标和取得的阶段性进展.该专题针对青藏高原地区农业生产中逐渐出现的局部农田生态系统结构失稳、功能失衡、地力衰退、作物品质下降、农残渐增等问题,在区域尺度上,从耕地肥力和土壤生物、农田植被、农业生产经营管理、作物产量品质和利用、农业气象条件等方面开展科学考察研究,在青藏高原农田生态系统相关生态环境参数的时空特征、分布规律与作物开发利用等考察研究方面取得阶段性重要进展,可为青藏高原农业生态系统可持续经营管理和粮食安全提供数据及科技支撑.(图1参90)     

Effects of different concentrations of tetracycline and oxytetracycline on the growth and ecotoxicity in lettuce北大核心CSCD

四环素类抗生素(TCs)是目前我国应用广泛、用量最大的一类抗生素,畜禽粪便和土壤中存在TCs残留,影响蔬菜作物的生长发育. TCs因水溶性较高更容易被植物转运和积累,植物对TCs耐受性机理研究仍不足.为更全面探究土壤TCs对蔬菜的毒性作用,研究不同浓度四环素(TC)和土霉素(OTC)分别对生菜的抗生素残留、生长特征及抗氧化酶系统的影响.结果显示,在0(对照)、2、10、50、250 mg/kg 5个施用水平下,生菜叶片抗生素含量逐渐增加,且土霉素含量始终大于四环素含量.与对照相比,抗生素浓度在50 mg/kg以上时对生菜生长具有显著抑制作用,其中,株高、根长、地上部和地下部鲜重与叶片TC残留量具显著负相关.生菜叶片的脯氨酸含量随浓度增加呈先增加后降低的趋势,在10 mg/kg时达到最大.并且低浓度(2 mg/kg)促进抗氧化酶基因SOD、POD21和CAT的表达,高浓度抗生素(50、250 mg/kg)对其产生抑制作用,10 mg/kg的抗生素处理对SOD、POD21和CAT基因表达的影响在抗生素种类上存在差异.本研究表明抗生素浓度超过50 mg/kg对生菜生长产生抑制作用,脯氨酸和抗氧化酶SOD、POD、CAT的转录水平及其酶活性能快速响应抗生素胁迫,可作为生菜对抗生素抗性的辅助评价指标.(图8表3参19)     

Population dynamics of farmland ladybug resources and dominant species in Nyingchi,Tibet, China北大核心CSCD

捕食性瓢虫是重要的天敌昆虫,有利于害虫的生物防治.为查清西藏林芝地区农田瓢虫资源与种群现状,明确优势种类及种群动态和规律,对林芝不同生态区域的青稞、小麦、油菜3种作物农田开展瓢虫资源调查研究,同时选取固定样地定期进行种群动态分析.结果显示:林芝地区农田瓢虫分为2个亚科4个族10个属,共13种,其中11种为捕食性瓢虫.主要优势瓢虫种类为横斑瓢虫、多异瓢虫、二星瓢虫.其中横斑瓢虫在全部调查样地中均有分布,在农田中总体优势度指数、相对多度为最高.3种优势瓢虫在不同作物中种群动态变化规律存在差异,其中横斑瓢虫虫口密度长期高于其余优势瓢虫,且与蚜虫密度曲线有较为明显的重合现象.横斑瓢虫种群密度峰值主要集中在6月中旬至7月上旬,但在不同作物农田中具体峰值时期存在差异.可见,林芝地区农田不同种类瓢虫之间有较大数量差异,在不同生态区域与作物环境下具有不同的种群特征,但是优势瓢虫种类与种群规律较为明确,可用于农田蚜虫生物防治;结果可为保护当地重要昆虫资源及开展生物防治提供基础数据支撑.(图4表6参23)     

Identification and expression analysis of the HvENOD93 gene family in barley北大核心CSCD

早期结瘤素93(ENOD93)蛋白在植物根瘤形成初期扮演着重要的角色.基于大麦基因组信息鉴定大麦ENOD93基因家族成员,分析其理化特性、进化关系、基因结构、蛋白质结构和启动子顺式作用元件;并分析ENOD93家族在不同组织和不同基因型(籽粒大小)的表达情况.结果显示,大麦ENOD93基因家族有16个成员,均含有ENOD93基因家族特有的保守结构域;编码区长度在207-627 bp之间,外显子数量有1-4个,平均2.75个,且大部分位于细胞膜上;进化树结果表明与水稻、小麦和玉米等禾本科植物ENOD93基因的亲缘关系较近;启动子顺式元件主要有植物生长发育响应元件、胁迫响应元件以及激素响应元件;大部分HvENOD93基因在灌浆期籽粒和大粒材料中表达量较高.推测大麦HvENOD93基因在籽粒大小形成中起关键性作用;另外,结合其他物种相关基因研究结果,共筛选出3个同源基因.(图4表2参45)     

Large-scale experiments for microbiological evaluation of measures for safeguarding sulfidic mine waste

In the framework of a German-Romanian scientific cooperation, experiments were performed to evaluate feasible and cheap techniques for the safe storage of mine waste to prevent acid rock drainage (ARD). A large four-chamber percolator (4CP) was installed in a waste heap at Ilba Mine, Romania, to test the effect of biocides and alkaline layers on the bacteria causing acid rock drainage (ARD). The 4CP consisted of four chambers each containing 65 m3 of sulfidic waste material. The 4CP enabled the transfer of laboratory results to a technical scale. The detergent sodiumdodecylsulfate (SDS) was proved to be active against the leaching bacteria. Organotrophic micro-organisms were not effected by the SDS application. The alkaline layers caused an increase of pH, however, a decrease of cell numbers was measured only in adjacent ore layers, but not in the whole ore body. A rapid evaluation of the effects of these countermeasures on ARD formation became possible by microcalorimetric activity measurements for bioleaching.