Distribution and ecology of leptocephali of the congrid eel, Ariosoma scheelei, around Sulawesi Island, Indonesia

A survey for leptocephali around Sulawesi Island in the central Indonesian Seas during May 2001 found that the leptocephali of the congrid eel, Ariosoma scheelei, were present in all seven areas that were sampled. A total of 551 leptocephali (22–166 mm TL) were collected, and A. scheelei was by far the most abundant species of leptocephali collected during the survey. The wide range of sizes in most areas indicated that spawning had occurred during a period of several months in many different areas, although the exact spawning locations were not determined. The larger size classes were more abundant in all areas except in Tomini Bay on the northeast side of Sulawesi Island. The highest catch rates were observed at the eastern edge of the Java Sea and to the north in the Celebes Sea near Makassar Strait. Premetamorphic leptocephali were also collected in surface samples at 11 stations (N=62), but metamorphosing leptocephali (N=86) were only caught in IKMT tows that fished from the surface to about 200 m. Metamorphosing leptocephali were collected primarily at two stations in the Java Sea and Makassar Strait where a surface layer of lower-salinity water was detected. Their total lengths (105.3–153.3 mm) and the largest premetamorphic individuals suggested that this species can reach maximum sizes of about 165 mm before beginning to metamorphose. It is hypothesized that this species may be abundant in the Indonesian Seas region and that it has ecological traits such as large size at recruitment and a small size at reproduction that have made it successful in many regions of the Indo-Pacific.     

Distribution of quinolone and macrolide resistance genes and their co-occurrence with heavy metal resistance genes in vegetable soils with long-term application of manure

The spread of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) has become an increasingly serious global public health issue. This study investigated the distribution characteristics and influencing factors of ARB and ARGs in greenhouse vegetable soils with long-term application of manure. Five typical ARGs, four heavy metal resistance genes (MRGs), and two mobile genetic elements (MGEs) were quantified by real-time quantitative polymerase chain reaction (qPCR). The amount of ARB in manure-improved soil greatly exceeded that in control soil, and the bacterial resistance rate decreased significantly with increases in antibiotic concentrations. In addition, the resistance rate of ARB to enrofloxacin (ENR) was lower than that of tylosin (TYL). Real-time qPCR results showed that long-term application of manure enhanced the relative abundance of ARGs in vegetable soils, and the content and proportion of quinolone resistance genes were higher than those of macrolide resistance genes. Redundancy analysis (RDA) showed that qepA and qnrS significantly correlated with total and available amounts of Cu and Zn, highlighting that certain heavy metals can influence persistence of ARGs. Integrase gene intI1 correlated significantly with the relative abundance of qepA, qnrS, and ermF, suggesting that intI1 played an important role in the horizontal transfer of ARGs. Furthermore, there was a weakly but not significantly positive correlation between specific detected MRGs and ARGs and MGEs. The results of this study enhance understanding the potential for increasing ARGs in manure-applied soil, assessing ecological risk and reducing the spread of ARGs.

Graphic abstract

     

县政府的责任与生态建设的理论及实践

生态县建设是实施可持续性战略的重要内容，在目前基础设施落后，资金短缺，技术力量不足的情况下，建设颇具复杂性、艰巨性，先进性和综合性的生态工程，是每个县政府的历史责任，生态建设理论的核心是社会－经济－自然复合生态系统理论、系统生态学理论，持续发展和同步建设理论三个方面的基本构架、近年来我国在农业生态工程、工业生态工程、水陆生态工程、生态县建设方面，取得了一定的经验。     

活性炭吸附水中铅离子的动态研究

本文采用两种煤质活性炭，研究了其对不同浓度二价铅离子溶液的吸附，应用固定床吸附动力学模型，Ｍａｒｑａｄｔ方法非线性回归固定床吸附流出曲线，获得了铅离子在活性炭上的扩散传质系数。结果发现，孔扩散系数Ｄｐ强裂地依赖于铅离子入口浓度，随着入口浓度的升高，孔扩散系数变小，随后用上述扩攻系数理论预测了其它操作条件下的流出曲线，结果表明实验曲线与理论预测曲线能很好地相符，动态法能可靠地获取固吸附过程的吸附及扩     

广州市污水污泥堆肥在环境绿化中的应用

将广州市大坦沙污水处理厂产生的脱水生污泥与粉煤灰、稻草按质量比例4∶1.2∶1混合堆制而成的污泥堆肥,堆肥总养分含量高于农家肥中的鸡粪,无论单独施用或与化肥结合使用应用于草坪等园林绿地,均有明显效果。污泥堆肥在草坪土壤应用的肥效试验表明,污泥堆肥与化肥结合使用效果较好,能缩短草坪草出苗和成坪时间,显著提高建植初期草坪草的生物量,但每公顷施用纯污泥堆肥30t与60t比较两者草坪草生物量没有显著差异;污泥堆肥施用污染风险初步分析表明,土壤中的重金属含量、渗滤液中的NO3--N和全P含量随着污泥堆肥施用量的增加而明显提高,考虑到广东地处热带、亚热带,雨量丰富且暴雨多,土壤呈酸性的特点,在园林绿地等相对封闭的体系中施用本项目堆制的污泥堆肥,只要将年施用量控制在30t/hm2以下,是基本安全的。     

Evolutionary relationships of deep-sea mussels inferred by mitochondrial DNA sequences

In order to elucidate the evolutionary process of deep-sea Bathymodiolus mussels, we investigated the phylogenetic relationships of 16 species worldwide by analyzing nucleotide sequences of the mitochondrial COI and ND4 genes. Deep-sea mussels were clustered into three groups by basal trichotomous divergence. The first was composed of four species found in Japanese waters and one species from the Gulf of Mexico, which contain methanotrophic endosymbiotic bacteria. The second included nine species distributed in the West and East Pacific, Indian, and Atlantic Oceans. Members of the second group were trichotomously divided into the Indo-West Pacific, Atlantic, and East Pacific subclusters. The Indo-West Pacific subcluster was composed of three very closely related species with mutual genetic distances at the intraspecific level (av. 0.019 in COI and 0.009 in ND4 relative to av. 0.156 in COI and 0.265 in ND4 among Bathymodiolus species other than Cluster A species), suggesting some gene flow among these species. The third consisted of two West Pacific species. Species in the second and third groups contain mainly thioautotrophic endosymbionts, including some species harboring both methanotrophs and thioautotrophs.     

王锦蛇的无冬眠饲养试验

2004年11月～2005年4月，进行了王锦蛇的冬眠期饲养试验．结果表明：在王锦蛇的冬眠期内，人工保持其环境温度为25～30℃，相对湿度为60％～85％，并提供其所需要的食物条件，就能够使王锦蛇在冬眠期内正常地生长发育，不仅能缩短其饲养时间，而且能提高其成活率，从而达到高效饲养王锦蛇的目的．     

3株真菌对毒死蜱的降解特性

从污水排放口污泥中分离到3株以毒死蜱为唯一碳源生长的真菌WZ-Ⅰ、WZ-Ⅱ、WZ-Ⅲ,鉴定均为镰孢霉属(FusariumLK. exFx). 3株菌5d内对50mgL-1毒死蜱的降解率分别高达93. 5%、91. 4%和83. 5%.测定了不同碳源、pH、温度及毒死蜱浓度对真菌降解能力和生长量的影响.结果表明,以毒死蜱为唯一碳源且其浓度为20~200mgL-1,pH6. 5~9. 0,温度30 ~40℃时,真菌的降解效果较好;真菌生长量随外加碳源浓度的增加而增加,在pH 6. 5 ~9. 0时生长量较大,且当毒死蜱浓度为50mgL-1,温度40℃时其生长量最大. 图5表1参18     

城市环境功能区环境功能评估方法

本文提出利用环境功能指数对城市环境功能区的环境功能进行评估，设计了相应的评估方法，以马鞍山市七个环境功能区为例进行了实例分析，讨论了各功能区的环境功能变化趋势，并提出了相应的对策与建议。     

天津污灌区苯并（a）芘、荧蒽和菲生态毒性的风险表征

运用概率风险评价方法表征和比较了天津污灌区3种多环芳烃对生物影响的风险性。根据各物质的环境暴露浓度和相应急性毒性值的累计概率分布曲线估计了各物质相对风险性的大小。采用联合概率曲线方式比较了不同暴露概率水平条件下3种多环芳烃的相对风险。结果表明，该地区目前菲的总体风险性高于另两种化合物，苯并（a)芘的总体风险性最低。而低暴露风险条件下（受威胁生物不超过20％），苯并（a)芘的风险较大，菲次之，荧蒽风险相对较低。