Effects of estrogen exposure in mussels, Mytilus edulis, at different stages of gametogenesis

Mytilus edulis were exposed to 17β-estradiol (E2) and the synthetic estrogens ethinyl estradiol (EE2) and estradiol benzoate (EB) for 10 days. Two exposures were performed to determine their effect on vitellogenin (VTG) and estrogen receptor 2 (ER2) mRNA expression at different stages of the reproductive cycle. Significant natural variation was not observed in VTG mRNA expression, though ER2 mRNA expression displayed significantly lower values during January, February and July compared with other times of the year. A significant increase in VTG and ER2 mRNA expression was observed in mussels exposed to estrogens at the early stage of gametogenesis. In contrast, mature mussels displayed no statistically significant change in the VTG or ER2 mRNA expression. The data presented suggests that the reproductive physiology of molluscs, in terms of VTG and ER2 mRNA expression, may be susceptible to damage by environmental estrogens at certain points in their gametogenesis process.     

Can juvenile corals be surveyed effectively using digital photography?: implications for rapid assessment techniques

Seasonal variations in the concentrations of groundwater with respect to arsenic, lead, cadmium and aluminium has been studied in Dhemaji district of Assam, India. The water samples were collected from 20 different sites in both dry and wet seasons. The metals were analysed by using atomic absorption spectrometer, Perkin Elmer AAnalyst 200 model. The concentrations of aluminium, lead and cadmium in groundwater were found to be significantly elevated. High concentrations of all the metals were recorded in the dry season than in the wet season. Univariate statistics along with skewness, kurtosis and confidence limit have been calculated for both the seasons to test the distribution normality for each metal. Statistical analyses of the data reveal non-uniform distribution of the metals in the area. The metal contamination of groundwater in the district follows the trend Al > Pb > Cd > As in both the seasons.     

Trends and events through seven centuries: the history of a wetland landscape in the Czech Republic

Environmental change can be viewed as the combined result of long-term processes and singular events. While long-term trends appear to be readily available for observation (in the form of temporal comparisons or space-for-time substitution), it is more difficult to gain information on singular events in the past, although these can be equally significant in shaping ecosystems. We examined the past 700 years in the history of a lowland wetland landscape in the Czech Republic with the help of palaeoecological, ecological, landscape archaeological, and archival data. Macrofossil and pollen data were compared to known drainage works in the area and historical climatological data. Trends and events in habitat conditions were assessed using species indicator values. Results showed that ecological succession was the general process in the study area, detected as a trend towards eutrophication, desiccation, and vegetation closure. Short-term events influenced development at the sites mainly from the second half of the nineteenth century. This is consistent with drainage history, although bias related to sample frequency cannot be excluded. On the whole, long-term trends and discrete events were complementary on different scales. We conclude that humans facilitated and accelerated background processes, which can be most likely associated with the succession of open wetlands towards terrestrial ecosystems.     

Zero-valent iron particles for PCB degradation and an evaluation of their effects on bacteria,plants, and soil organisms

Two types of nano-scale zero-valent iron (nZVI-B prepared by borohydride reduction and nZVI-T produced by thermal reduction of iron oxide nanoparticles in H₂) and a micro-scale ZVI (mZVI) were compared for PCB degradation efficiency in water and soil. In addition, the ecotoxicity of nZVI-B and nZVI-T particles in treated water and soil was evaluated on bacteria, plants, earthworms, and ostracods. All types of nZVI and mZVI were highly efficient in degradation of PCBs in water, but had little degradation effect on PCBs in soil. Although nZVI-B had a significant negative impact on the organisms tested, treatment with nZVI-T showed no negative effect, probably due to surface passivation through controlled oxidation of the nanoparticles.

     

The use of phosphomannose isomerase selection system for Agrobacterium-mediated transformation of tobacco and flax aimed for phytoremediation

A plant selection system based on the phosphomannose isomerase gene (pmi) as a selectable marker is often used to avoid selection using antibiotic resistance. Nevertheless, pmi gene is endogenous in several plant species and therefore difficult to use in such cases. Here we evaluated and compared Agrobacterium-mediated transformation of Linum usitatissimum breeding line AGT-952 (without endogenous pmi gene) and Nicotiana tabacum var. WSC-38 (with endogenous pmi gene). Transformation was evaluated for vectors bearing transgenes that have the potential to be involved in improved phytoremediation of contaminated environment. Tobacco regenerants selection resulted in 6.8% transformation efficiency when using a medium supplemented with 30 g/L mannose with stepwise decrease of the sucrose concentration. Similar transformation efficiency (5.3%) was achieved in transformation of flax. Relatively low selection efficiency was achieved (12.5% and 34.8%, respectively). The final detection of efficient pmi selection was conducted using PCR and the non-endogenous genes; pmi transgene for flax and todC2 transgene for tobacco plants.     

Do remediation experts have what it takes to explain empirical uncertainty?

Words matter in risk communication, and experts’ choice of words is critical when remediation risks are being explained to nonexperts. In risk communication studies, communication gaps between experts and nonexperts are investigated but there is lack of primary research. An Australian project addresses this shortcoming through research into communication about the risks of contaminated land remediation, and this paper provides some of its findings. Seventeen experts completed a questionnaire about the meaning of some scientific terms, and analysis found that they have capacity to improve communication through their selection and use of language. When experts undertake risk communication, the language they use may increase or reduce communication gaps. When the topic is uncertainty about health risks, communication gaps about the extent of uncertainty may reduce the effectiveness of social engagement, leading to unintended consequences such as cost overruns. This situation makes for a good case study since remediation is about benefit as well as risk, and communication about benefit, while desirable, may not always be achievable. The study suggests how to improve risk communication by exploring the accuracy, clarity, and depth of expert language. It identifies attributes of language that can bridge gaps in knowledge and understanding and characterizes them as integration mechanisms. These are defined as knowledge forms and mental processes that support cooperation between different epistemic communities to achieve mutually agreed outcomes. Two integration mechanisms are suggested. Bridging content addresses communication gaps through the selection of content (what knowledge is selected). Bridging process addresses communication gaps through the use of language (how knowledge is explained). Bridging content and bridging process can be expressed through cognitive and experiential platforms, or a blending of both, so whether words are positioned in the science‐based or mental model of risk communication, a utility value can be found in their quality, whether reflected by accuracy or the power to communicate meaning.     

Managing water services in tropical regions: From land cover proxies to hydrologic fluxes

Watershed investment programs frequently use land cover as a proxy for water-based ecosystem services, an approach based on assumed relationships between land cover and hydrologic outcomes. Water flows are rarely quantified, and unanticipated results are common, suggesting land cover alone is not a reliable proxy for water services. We argue that managing key hydrologic fluxes at the site of intervention is more effective than promoting particular land-cover types. Moving beyond land cover proxies to a focus on hydrologic fluxes requires that programs (1) identify the specific water service of interest and associated hydrologic flux; (2) account for structural and ecological characteristics of the relevant land cover; and, (3) determine key mediators of the target hydrologic flux. Using examples from the tropics, we illustrate how this conceptual framework can clarify interventions with a higher probability of delivering desired water services than with land cover as a proxy.

Electronic supplementary material

The online version of this article (doi:10.1007/s13280-014-0578-8) contains supplementary material, which is available to authorized users.     

When the going gets tough: Employee reactions to large-scale organizational change and the role of employee Machiavellianism

Large-scale, long-term change initiatives take time to unfold, which can be a source of uncertainty and strain. Investigating the initial 19 months of a large-scale change, we argue that during these stages, employees' change-related beliefs become more negative over time, which negatively affects their work engagement and, ultimately, increases their turnover intentions. Furthermore, we investigate the impact of a trait, Machiavellianism, on change reactions and propose that employees high in Machiavellianism react more negatively during change processes as they are especially susceptible to uncertainty and stress. We test our (cross-level) moderated mediation model in a three-wave longitudinal study among employees undergoing a large-scale change (T1: n = 1,602; T2: n = 1,113; T3: n = 759). We find that employees' beliefs about the impact and value of the change are indeed negatively related to change duration and that decreases in these perceptions come with a decline in engagement and increases in turnover intentions. Moreover, employees high in Machiavellianism react more strongly to a deterioration in change-related beliefs, showing stronger reductions in engagement and stronger increases in turnover intentions than employees low in Machiavellianism. Our study offers explanations for the negative effects of large-scale changes including an explanatory factor for disparate employee reactions to change over time.