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941.
942.
While true mosaicism occurs in only 0–25 per cent of genetic amniocenteses, nearly 2–5 per cent of amniotic fluid cell cultures contain a second cell line. In the common practice of prenatal diagnosis, an aberrant cell line confined to a single colony is usually disregarded. We present a case of mosaic trisomy 14 which was not detected on initial chromosome analysis. At birth, multiple malformations were apparent. Newborn cytogenetic studies revealed mosaicism [46,XX/46,XX,-14,+i(14q)] with an isochromosome 14 in 37 per cent of lymphocytes. Additional cells from the initial amniotic fluid culture were analysed post-delivery and the isochromosome 14 identified in only one of 12 total colonies. This case illustrates two important lessons in prenatal diagnosis. First, amniotic fluid cell cultures may not accurately reflect the relative distribution of the normal and abnormal cell lines within a mosaic fetus. Second, while it is generally reasonable to disregard mosaicism confined to a single colony, this policy will, on rare occasion, result in diagnostic error. This should be taken into consideration, particularly when dealing with autosomal trisomies potentially compatible with livebirth. 相似文献
943.
944.
Keri L. Christensen Anthony P. Gallacher Lizzie Martin Desmond Tong Mark A. Elgar 《Die Naturwissenschaften》2010,97(10):941-944
The geometric framework model predicts that animal foraging decisions are influenced by their dietary history, with animals targeting a combination of essential nutrients through compensatory foraging. We provide experimental confirmation of nutrient-specific compensatory foraging in a natural, free-living population of social insects by supplementing their diet with sources of protein- or carbohydrate-rich food. Colonies of the ant Iridomyrmex suchieri were provided with feeders containing food rich in either carbohydrate or protein for 6 days, and were then provided with a feeder containing the same or different diet. The patterns of recruitment were consistent with the geometric framework: while feeders with a carbohydrate diet typically attracted more workers than did feeders with protein diet, the difference in recruitment between the two nutrients was smaller if the colonies had had prior access to carbohydrate than protein. Further, fewer ants visited feeders if the colony had had prior access to protein than to carbohydrates, suggesting that the larvae play a role in worker foraging behaviour. 相似文献
945.
946.
Hopanoids are pentacyclic triterpenoid lipids occurring in bacteria. They are synthesized from isopentenyl units which are
formed in a new biosynthetic route leading to isopentenyl diphosphate. Six C5 units are joined to form squalene, the immediate precursor in hopanoid synthesis. In a highly complex cyclization reaction
that shares considerable similarities with that of oxidosqualene to sterols, the hopane skeleton is formed from squalene by
the squalene-hopene cyclase. Recent elucidation of the X-ray structure of this membrane-bound cyclase has shed some light
on the properties of this unusual enzyme. The active site is located in a cavity within the enzyme. The squalene substrate
diffuses through a channel structure from the membrane into this cavity and is there transformed into hopene. Polar side chains
are attached to hopene resulting in the amphiphilic molecular structure of many hopanoids. These hopanoids are membrane components
involved in regulating membrane fluidity and stability. However, the many structural variants of hopanoids indicate that they
may have other interesting but as yet unknown functions. 相似文献
947.
Laura Y. Sifuentes Sonia L. M. Fankem Kelly Reynolds Akrum H. Tamimi Charles P. Gerba David Koenig 《Food and environmental virology》2017,9(1):14-19
The purpose of this study was to validate the use of adenosine triphosphate (ATP) for evaluating hygiene intervention effectiveness in reducing viral dissemination in an office environment. The bacterial virus MS-2 was used to evaluate two scenarios, one where the hand of an individual was contaminated and another where a fomite was contaminated. MS-2 was selected as a model because its shape and size are similar to many human pathogenic viruses. Two separate experiments were conducted, one in which the entrance door push plate was inoculated and the other in which the hand of one selected employee was inoculated. In both scenarios, 54 selected surfaces in the office were tested to assess the dissemination of the virus within the office. Associated surface contamination was also measured employing an ATP meter. More than half of the tested hands and surfaces in the office were contaminated with MS-2 within 4 h. Next, an intervention was conducted, and each scenario was repeated. Half of the participating employees were provided hand sanitizer, facial tissues, and disinfecting wipes, and were instructed in their use. A significant (p < 0.05) reduction was observed in the number of surfaces contaminated with virus. This reduction in viral spread was evident from the results of both viral culture and the surface ATP measurements, although there was no direct correlation between ATP measurements with respect to viral concentration. Although ATP does not measure viruses, these results demonstrate that ATP measurements could be useful for evaluating the effectiveness of hygiene interventions aimed at preventing viral spread in the workplace. 相似文献
948.
Data on the existence of a specific system regulating the growth, size, and structure of enterobacterial populations are first summarized in terms of the chemical ecology concept formulated by S.S. Shvarts. The role of this system in transgene functioning is described using the example of the lux operon integrated into Escherichia coli. A relatively simple test for estimating such interactions is proposed.Translated from Ekologiya, No. 2, 2005, pp. 91–96.Original Russian Text Copyright © 2005 by Pshenichnov, Maslennikova, Nikitina. 相似文献
949.
On-going population growth and resulting domestic demand for water require rapid and effective decision-making as regards groundwater management and control of the various sources of salinization and pollution in Coastal aquifers. Sustainability of water resources for utilization by future generations must therefore be a high priority, not only for the purpose of fulfilling needs for water usage but also for bringing people into harmony with their ambient natural environment.The objective of this paper is to propose an empirical approach for prioritization of the needs involved for sustainable aquifer management. The approach involves a schematic format to:(1) develop a global understanding of an aquifer's hydrological and environmental properties in order to delineate appropriate eco-hydrological scenarios and recommend corresponding operational management activities; and(2) emphasize the importance of educating and increasing the awareness of the population involved as to the need for and viability of socially acceptable measures for sustainable management of groundwater and other resources.The psychologist Abraham Maslow utilized a pyramid to illustrate that until people's most basic needs were fulfilled, higher levels of needs would remain irrelevant. This paper postulates a comparable pyramid prioritizing hydrological needs required for progressing towards sustainable groundwater resources. Two sub-regions of Israel's Coastal aquifer in the Sharon region have been presented as representative areas, each characterized by different stress of exploitation. In assessing these sub-regions situation, specific measures have been recommended for achieving and/or maintaining sustainable groundwater resources in light of the ambient environment, and the level of the population on the pyramidal hierarchy of groundwater needs. 相似文献
950.
Estuarine sediments from a USEPA Superfund site in coastal Georgia were extensively contaminated with Aroclor 1268, a mixture of highly chlorinated polychlorinated biphenyls used by a former chlor-alkali plant. Batch slurries of contaminated sediment were incubated for 1 yr with amendments of 2,6-dibromobiphenyl (26-BB) and 2,3,4,5,6-pentachlorobiphenyl (23456-CB) under anaerobic, sulfate-reducing conditions and different pH (5.5-7.5). Organic extracts of slurry sub-samples in a time series were analyzed by congener-specific GC-MS. Dechlorination of 23456-CB was pH dependent and occurred via two routes with the sequential loss of (1) meta and para chlorines and (2) para, ortho, and meta chlorines. Quantitative dehalogenation of 26-BB was observed at all pH. Supplementation of nonachlorobiphenyls (as primers) did not induce dechlorination of native Aroclor 1268 nor of the primers themselves. While contaminated estuarine sediments possess microbial consortia with diverse dehalogenating activities, lack of dechlorination of Aroclor 1268 and spiked nonachlorobiphenyl congeners suggests a bioavailability limitation or enzyme-substrate incompatibilities. 相似文献