Nutzung der induzierbaren Genexpression des NematodenCaenorhabditis elegans als Biomonitor

The soil nematodeCaenorhabditis elegans is one of the simplest animals having the status of a laboratory model. Its already completely sequenced genome contains the remarkable number of 80 cytochrome P450 genes (CYP) and many further genes coding for enzymes involved in biotransformation. In order to study xenobiotically induced gene expression inC. elegans, liquid cultures were exposed to different, well-known xenobiotic inducers. The mRNA expression was detected by two different types of DNA arrays and semi-quantitative RT-PCR. β-naphthoflavone, PCB52 and lansoprazol were the most active and, in particular, induced almost all CYP35 isoforms strongly. In conclusion, the xenobiotic dependent gene expression ofC. elegans is a useful tool to reveal defense mechanisms against potential damaging substances as well as for developing a biomonitoring system.     

STATISTICAL ANALYSIS OF WATER CONSUMPTION1

The purpose of this study was to determine the degree of influence of various factors on municipal water consumption in Illinois. For the collection of basic data, questionnaires were sent to all public water works of incorporated towns. The questionnaire was designed to obtain information on factors which may have any effect on water use. The effects of the different parameters on water consumption were based on several correlation and regression combinations of predictands and predictors. It was found that in the Chicago region the percent of services and water used for commercial and industrial purposes and the age of the water works were the most important parameters influencing water consumption (gallons per capita per day) when pumpage is metered at the water works as well as at the customers. For the State, excluding the Chicago region, percent of public water use, persons per service, population and commercial and industrial water use were the most important parameters. It has been recommended that similar statistical analysis be conducted periodically to establish a trend or law of change from the influencing parameters.     

Portrayal of sustainability principles in the mission statements and on home pages of the world's largest organizations

Conservation can be achieved only if sustainability is embraced as core to organizational cultures. To test the extent to which the related concepts of sustainability, conservation, response to climate change, poverty alleviation, and gender equity have been incorporated into organizational culture, we compared mission statements published from 1990 to 2000 with those published in 2014 for 150 organzations, including conservation nongovernmental organizations (NGOs), aid NGOs, government development agencies, resource extraction companies, and retailers (30 in each category). We also analyzed the 2014 home web pages of each organization. Relative to the earlier period, the frequency with which mission statements mentioned poverty alleviation, biodiversity conservation, and a range of sustainable practices increased only slightly by 2014, particularly among resource extractors and retail companies. Few organizations in any sector had embedded either climate change or gender equity into their mission statements. In addition, the proportional intensity with which any of the aspirations were expressed did not change between periods. For current home pages, conservation NGOs, resource extractors, and government agencies were significantly more likely to acknowledge the importance of matters that were not part of their core business, but few aid agencies or retail companies promoted goals beyond alleviation of crises and profit maximization, respectively. Overall, there has been some progress in recognizing poverty alleviation, biodiversity conservation, and sustainable practices, but gender equity and a determination to reduce impacts on climate change are still rarely promoted as central institutional concerns. Sustainability in general, and biodiversity conservation in particular, will not be achieved unless their importance is more widely apparent in core communication products of organizations.     

The importance of Indigenous Peoples’ lands for the conservation of terrestrial mammals

Indigenous Peoples’ lands cover over one-quarter of Earth's surface, a significant proportion of which is still free from industrial-level human impacts. As a result, Indigenous Peoples and their lands are crucial for the long-term persistence of Earth's biodiversity and ecosystem services. Yet, information on species composition on these lands globally remains largely unknown. We conducted the first comprehensive analysis of terrestrial mammal composition across mapped Indigenous lands based on data on area of habitat (AOH) for 4460 mammal species assessed by the International Union for Conservation of Nature. We overlaid each species’ AOH on a current map of Indigenous lands and found that 2695 species (60% of assessed mammals) had ≥10% of their ranges on Indigenous Peoples’ lands and 1009 species (23%) had >50% of their ranges on these lands. For threatened species, 473 (47%) occurred on Indigenous lands with 26% having >50% of their habitat on these lands. We also found that 935 mammal species (131 categorized as threatened) had ≥ 10% of their range on Indigenous Peoples’ lands that had low human pressure. Our results show how important Indigenous Peoples’ lands are to the successful implementation of conservation and sustainable development agendas worldwide.     

Deliquescence of Deposited Atmospheric Particles on Leaf Surfaces

A large fraction of deposited aerosol particles on leafsurfaces represents hygroscopic material in a high humidity environment, likely to become deliquescent within the water vapour transpired by the leaf. Microscopic observations on leaf surfaces of beech, kohlrabi and elder leaves grown in a particle-free environment and/or treated with defined particle load are presented. Spreading of deliquescent particles, formation of salt crusts, and encoating of and by waxes was observed. Deliquescence of NaNO₃ particles (deliquescence point 74% RH at 25 °C) was observed on and near the stomata at 35% relative humidity (RH) of the surrounding air, illustrating the influence of the boundary layer. Aerosols are difficult to identify on leaf surfaces, as similar patterns may be produced by deliquescent particles and processes like salt creep. These problems are especially important on leaves from the natural environment if only scanning electron microscopy (SEM) is used. These patterns could appear similar to the fused waxes described in forest decline research. Strong gradients are formed between deliquescent particles and the apoplastic solution within the leaf, promoting cuticular and stomatal uptake.     

Comparison of microbial pyrene and benzo[a]pyrene mineralization in liquid medium, soil slurry, and soil

The microbial degradation of 14C-pyrene and 14C-benzo[a]pyrene by a bacterial mixed culture was studied within a mixture of the PAHs phenanthrene, anthracene, pyrene, fluoranthene, and benzo[a]pyrene as sole carbon source in the different culture systems: (i) liquid medium, (ii) soil slurry (surface and grinding influence), and (iii) soil. The fate of these two labeled compounds was followed in these systems with an emphasis on mineralization to carbon dioxide, extractability, and adsorption to humic materials and formation of unextractable residual. Mineralization showed the most obvious differences: soil slurries achieved the best results both concerning the extent of mineralization and the time required. The highest extent of pyrene mineralization (54% within 21 days) was observed in soil slurries; in liquid media, pyrene mineralization was slower, but reached approximately the same extent (54% in 150 days); in soils, mineralization reached only 36% of added pyrene after 160 days. Benzo[a]pyrene was mineralized in a mixture of PAHs in soil slurries to an extent of 34% within 70 days, whereas mineralization in liquid medium and soil occurred in the range of 5% (70 days). Mineralization of benzo[a]pyrene in sand slurries was lower compared to soil slurries (19% in sand slurries vs. 32% in soil slurries within 50 days).     

Multivariate data analysis of organochlorines and brominated flame retardants in Baltic Sea guillemot (Uria aalge) egg and muscle

Concentrations of organochlorines (OCs) and brominated flame-retardants (BFRs) were determined in guillemot (Uria aalge) eggs from the island of Stora Karls? in the Baltic Sea where 10 eggs/year were collected in the years 2000, 2001 and 2002. The dominating contaminant in egg was p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) with a geometric mean (GM) concentration of 18200 ng/g lipid weight (lw). The GM concentration in egg of hexabromocyclododecane (HBCD) of 140 ng/glw, was significantly higher than that of polybrominated diphenyl ether (summation operatorPBDE) of 77 ng/glw. For the evaluation of the data multivariate data analysis techniques namely principal components analysis (PCA), partial least squares regression (PLS), soft independent modelling of class analogy (SIMCA classification), and PLS discriminant analysis (PLS-DA), were used. We investigated whether the eggs' biological characteristics co-varied with egg concentrations of OCs and BFRs, and found e.g., significant negative correlations between egg weight and concentrations of HCB and p,p'-DDE. A PLS model with analyzed BFRs as the Y matrix and OCs as the X matrix could, with varying accuracy, calculate the concentrations of BFRs in the individual egg from their concentrations of OCs (e.g., R(2)Y of 0.89 for BDE47, and of 0.50 for HBCD). Lastly, we compared the contaminant concentrations in the eggs to those in previously analyzed pectoral muscles from adult guillemots from Stora Karls?, from the year 2000. A PLS-DA model, showed that some of the contaminants (e.g., HBCD and CB28) had significantly higher concentrations in egg than in muscle, although 7 of the 14 contaminants showed no difference in concentrations between the two matrices.     

斯德哥尔摩群岛水质历史上的城市影响

从历史的角度看,斯德哥尔摩市居民人数的增长对周围湖泊和沿岸地区的水质产生了负面影响.自19世纪中叶以来,政府的治理和为改善水质所采取的措施一直在进行中.由于采样很少,而且使用了不同的参数和方法,因此很难根据首次化学和生物调查结果来评估与以后若干年相比较的19世纪的水况.但是,可以根据开始于20世纪初的浮游生物调查的结果对水质进行一次回顾性评价.在具有与整个20世纪相似温度条件的夏季对反映富营养状况的蓝藻Planktothrixagardhii的发生进行了调查.调查结果表明,从20世纪初到90年代初.在斯德哥尔摩群岛,富营养化条件可能一直占优势.20世纪的最后10年,水质似乎比l00年前要好.今天较好的水况很可能是在度水处理方面采取适当措施的结果.     

Airborne PCB as a contamination risk on freeze-drying of sediment

In a study of polychlorinated biphenyls (PCB) in freeze-dried sediments from Swedish lakes, the samples were found to contain unexpectedly high concentrations of PCBs. In one of the lakes (Bj?rken), e.g. the concentration of PCB-28 was found to be 189 ng/g dw after freeze-drying compared to 7 ng/g dw after air-drying of the sediment and therefore our hypothesis was that the contamination had occurred during the freeze-drying procedure. Hence, a dry reference sediment (RS) was used in order to identify potential sources of PCB contamination. The investigation included freeze-drying of the dry RS in five different laboratories, exposure to the air in the freeze-drying room, storage at room temperature and air-drying in a certain fume hood and in a laminar flow clean bench. The pattern and concentrations of PCBs detected in the exposed RS were strongly influenced by low chlorinated PCB congeners under all of these conditions with exception for air-drying in the fume hood. Storage in the laboratory air resulted in a slight elevation in the concentrations of low chlorinated PCB congeners, whereas higher concentrations of all PCB congeners were observed after freeze-drying. In all experiments the contamination appeared to be due to adsorption of PCB from the laboratory air. On the basis of these findings we recommend that reference material be subjected to the entire procedure, including sample preparation, in order to monitor contamination and that a procedure involving solvent extraction of wet sediment samples is used, whenever possible, instead of procedures involving dry samples.     

Expression profiling of five different xenobiotics using a Caenorhabditis elegans whole genome microarray

The soil nematode Caenorhabditis elegans is frequently used in ecotoxicological studies due to its wide distribution in terrestrial habitats, its easy handling in the laboratory, and its sensitivity against different kinds of stress. Since its genome has been completely sequenced, more and more studies are investigating the functional relation of gene expression and phenotypic response. For these reasons C. elegans seems to be an attractive animal for the development of a new, genome based, ecotoxicological test system. In recent years, the DNA array technique has been established as a powerful tool to obtain distinct gene expression patterns in response to different experimental conditions. Using a C. elegans whole genome DNA microarray in this study, the effects of five different xenobiotics on the gene expression of the nematode were investigated. The exposure time for the following five applied compounds beta-NF (5 mg/l), Fla (0.5 mg/l), atrazine (25 mg/l), clofibrate (10 mg/l) and DES (0.5 mg/l) was 48+/-5 h. The analysis of the data showed a clear induction of 203 genes belonging to different families like the cytochromes P450, UDP-glucoronosyltransferases (UDPGT), glutathione S-transferases (GST), carboxylesterases, collagenes, C-type lectins and others. Under the applied conditions, fluoranthene was able to induce most of the induceable genes, followed by clofibrate, atrazine, beta-naphthoflavone and diethylstilbestrol. A decreased expression could be shown for 153 genes with atrazine having the strongest effect followed by fluoranthene, diethylstilbestrol, beta-naphthoflavone and clofibrate. For upregulated genes a change ranging from approximately 2.1- till 42.3-fold and for downregulated genes from approximately 2.1 till 6.6-fold of gene expression could be affected through the applied xenobiotics. The results confirm the applicability of the gene expression for the development of an ecotoxicological test system. Compared to classical tests the main advantages of this new approach will be the increased sensitivity and it's potential for a substance class specific effect determination as well as the large numbers of genes that can be screened rapidly at the same time and the selection of well regulated marker genes to study more in detail.