Improvement of Impact Toughness of Biodegradable Poly(butylene succinate) by Melt Blending with Sustainable Biobased Glycerol Elastomers

Poly(butylene succinate) (PBS) was melt blended with glycerol based polyesters (PGS) synthesized from pure and technical glycerol aiming to improve the impact strength of PBS. It was found that after addition of 30 wt% PGS to PBS its impact strength was significantly increased by 344% (from 31.9 to 110 J/m) and its elongation at break was maintained at 220%. Infrared spectra of the blends showed the presence of hydroxyl groups from the PGS phase suggesting that hydrogen bonding between the phases could be responsible for a good stress transfer and an efficient toughening in the PBS/PGS blends. Scanning electron microscopy imaging showed a good dispersion of PGS phase into PBS with a PGS particle size of 10 μm and less and no agglomeration. Addition of PGS to PBS was shown to be an effective strategy for improvement of PBS impact resistance without serious detrimental effects on its thermal and rheological properties.     

Investigating toxic metal levels in popular edible fishes from the South Durban basin: implications for public health and food security

Contamination of the ocean by heavy metals may have ecosystem-wide implications because they are toxic even if present in trace levels, and the relative ease of their bioaccumulation by marine organisms may affect human health, primarily through consumption of contaminated fish. We evaluated metal concentrations in six different popular edible fish species and estimated the potential health risks from consumption of contaminated fish. There was no correlation between fish length and average metal accumulation although the fish species tended to accumulate significantly more Al and Zn (P?<?0.05) than any of the other metals. Significantly higher Mn concentrations were found in fish gills compared to other body parts in all fish species. Bronze seabream, Catface rockcod, and Slinger seabream had significantly higher mean Cr concentration in the liver than in either the tissues or gills. The highest concentration of Zn in fleshy tissue was in Horse mackerel (56.71 μg g^?1) followed by Bronze seabream (31.07 μg g^?1). Al levels ranged from 5.6 μg g^?1 in Atlantic mackerel to 35.04 μg g^?1 in Horse mackerel tissue while Cu and Cr concentrations were highest in the tissues of Horse mackerel (6.83 and 1.81 μg g^?1, respectively) followed by Santer seabream (3.15; 1.09 μg g^?1) and Bronze seabream (3.09; 1.30 μg g^?1), respectively. The highest tissue concentration of Mn was detected in Bronze seabream (8.23 μg g^?1) followed by Catface rockcod (6.05 μg g^?1) and Slinger seabream (5.21 μg g^?1) while Pb concentrations ranged from a high of 8.44 μg g^?1 in Horse mackerel to 1.09 μg g^?1 in Catface rockcod. However, the estimated potential health risks from fish consumption as determined by the target hazard quotient (THQ) and hazard index (HI) were significantly lower than 1, implying that metals were not present in sufficiently high quantities to be of any health and/or food and security concern in the studied fishes.     

The effects of temperature and salinity on reproductive success of <Emphasis Type="Italic">Temora longicornis</Emphasis> in the Baltic Sea: a copepod coping with a tough situation

At specific locations within the Baltic Sea, thermoclines and haloclines can create rapid spatial and temporal changes in temperature (T) and salinity (S) exceeding 10°C and 9 psu with seasonal ranges in temperature exceeding 20°C. These wide ranges in abiotic factors affect the distribution and abundance of Baltic Sea copepods via species-specific, physiological-based impacts on vital rates. In this laboratory study, we characterized the influence of T and S on aspects of reproductive success and naupliar survival of a southwestern Baltic population of Temora longicornis (Copepoda: Calanoida). First, using ad libitum feeding conditions, we measured egg production (EP, no. of eggs female⁻¹ day⁻¹) at 12 different temperatures between 2.5 and 24°C, observing the highest mean EP at 16.9°C (12 eggs female⁻¹ day⁻¹). Next, the effect of S on EP and hatching success (HS, %) was quantified at 12°C for cohorts that had been acclimated to either 8, 14, 20 or 26 psu and tested at each of five salinities (8, 14, 20, 26 and 32 psu). The mean EP was highest for (and maximum EP similar among) 14, 20 and 26 psu cohorts when tested at their acclimation salinity whereas EP was lower at other salinities. For adults reared at 8 psu, a commonly encountered salinity in Baltic surface waters, EP was relatively low at all test salinities—a pattern indicative of osmotic stress. When incubated at 12°C and 15 different salinities between 0 and 34 psu, HS increased asymptotically with increasing S and was maximal (82.6–84.3%) between 24 and 26 psu. However, HS did depend upon the adult acclimation salinity. Finally, the 48-h survival of nauplii hatched and reared at 14 psu at one of six different temperatures (10, 12, 14, 16, 18 and 20°C) was measured after exposure to a novel salinity (either 7 or 20 psu). Upon exposure to 7 psu, 48-h naupliar mortality increased with increasing temperature, ranging from 26.7% at 10°C to 63.2% at 20°C. In contrast, after exposure to 20 psu, mortality was relatively low at all temperatures (1.7% at 10°C and ≤26.7% for all other temperatures). An intra-specific comparison of EP for three different T. longicornis populations revealed markedly different temperature optima and clearly demonstrated the negative impact of brackish (Baltic) salinities. Our results provide estimates of reproductive success and early survival of T. longicornis to the wide ranges of temperatures and salinities that will aid ongoing biophysical modeling examining climate impacts on this species within the Baltic Sea.     

Removal of malachite green from water samples by cloud point extraction using Triton X-100 as non-ionic surfactant

A surfactant mediated cloud point extraction (CPE) procedure has been developed to remove color from wastewater containing malachite green using Triton X-100 as non-ionic surfactant. The effects of the concentration of the surfactant, temperature and salt concentration on the different concentrations of dye have been studied and optimum conditions were obtained for the removal of malachite green. The concentration of malachite green in the dilute phase was measured using UV–Vis spectrophotometer. It was found that the separation of phases was complete and the recovery of malachite green was very effective in the presence of NaCl as an electrolyte. The results showed that up to 500 ppm of malachite green can quantitatively be removed (>95%) by CPE procedure in a single extraction using optimum conditions.     

In-hive patterns of temporal polyethism in strains of honey bees (Apis mellifera) with distinct genetic backgrounds

Honey bee workers exhibit an age-based division of labor (temporal polyethism, DOL). Younger bees transition through sets of tasks within the nest; older bees forage outside. Components of temporal polyethism remain unrevealed. Here, we investigate the timing and pattern of pre-foraging behavior in distinct strains of bees to (1) determine if a general pattern of temporal DOL exists in honey bees, (2) to demonstrate a direct genetic impact on temporal pacing, and (3) to further elucidate the mechanisms controlling foraging initiation. Honey bees selected for differences in stored pollen demonstrate consistent differences in foraging initiation age. Those selected for increased pollen storage (high pollen hoarding strain, HSBs) initiate foraging earlier in life than those selected for decreased pollen storage (low pollen hoarding strain, LSBs). We found that HSBs both initiate and terminate individual pre-foraging tasks earlier than LSBs when housed in a common hive environment. Unselected commercial bees (wild type) generally demonstrated intermediate behavioral timing. There were few differences between genotypes for the proportion of pre-foraging effort dedicated to individual tasks, though total pre-foraging effort differences differed dramatically. This demonstrates that behavioral pacing can be accelerated or slowed, but the pattern of behavior is not fundamentally altered, suggesting a general pattern of temporal behavior in honey bees. This also demonstrates direct genetic control of temporal pacing. Finally, our results suggest that earlier HSB protein (pollen) consumption termination compared to LSBs may contribute to an earlier decline in hemolymph vitellogenin protein titers, which would explain their earlier onset of foraging.     

Greenhouse gas emissions during co-composting of calf mortalities with manure

Composting may be a viable on-farm option for disposal of cattle carcasses. This study investigated greenhouse gas emissions during co-composting of calf mortalities with manure. Windrows were constructed that contained manure + straw (control compost [CK]) or manure + straw + calf mortalities (CM) using two technologies: a tractor-mounted front-end loader or a shredder bucket. Composting lasted 289 d. The windrows were turned twice (on Days 72 and 190), using the same technology used in their creation. Turning technology had no effect on greenhouse gas emissions or the properties of the final compost. The CO2 (75.2 g d(-1) m(-2)), CH4 (2.503 g d(-1) m(-2)), and N2O (0.370 g d(-1) m(-2)) emissions were higher (p < 0.05) in CM than in CK (25.7, 0.094, and 0.076 g d(-1) m(-2) for CO2, CH4, and N2O, respectively), which reflected differences in materials used to construct the compost windrows and therefore their total C and total N contents. The final CM compost had higher (p < 0.05) total N, total C, and mineral N content (NO3*+ NO2* + NH4+) than did CK compost and therefore has greater agronomic value as a fertilizer.     

Consequences of increased terrestrial dissolved organic matter and temperature on bacterioplankton community composition during a Baltic Sea mesocosm experiment

Predicted increases in runoff of terrestrial dissolved organic matter (DOM) and sea surface temperatures implicate substantial changes in energy fluxes of coastal marine ecosystems. Despite marine bacteria being critical drivers of marine carbon cycling, knowledge of compositional responses within bacterioplankton communities to such disturbances is strongly limited. Using 16S rRNA gene pyrosequencing, we examined bacterioplankton population dynamics in Baltic Sea mesocosms with treatments combining terrestrial DOM enrichment and increased temperature. Among the 200 most abundant taxa, 62 % either increased or decreased in relative abundance under changed environmental conditions. For example, SAR11 and SAR86 populations proliferated in combined increased terrestrial DOM/temperature mesocosms, while the hgcI and CL500-29 clades (Actinobacteria) decreased in the same mesocosms. Bacteroidetes increased in both control mesocosms and in the combined increased terrestrial DOM/temperature mesocosms. These results indicate considerable and differential responses among distinct bacterial populations to combined climate change effects, emphasizing the potential of such effects to induce shifts in ecosystem function and carbon cycling in the future Baltic Sea.     

Distribution characteristics of PCBs in the sediments of the lower Nakdong River, Korea

The objective of this study is to investigate the distribution characteristics of polychlorinated biphenyl (PCB) congeners in sediments of the lower Nakdong River of Korea. The sediments were collected using a grab sampler in May 1999. PCBs were extracted in the Soxhlet extractor and cleaned by using adsorption chromatographic techniques and concentrated sulfuric acid. A HP6890 gas chromatograph equipped with an electron capture detector was used for quantification. The results of congener specific analysis showed that the total concentration is ranged over 1.1-141 ng/g dry weight. The levels of PCB congeners are significantly correlated with the total organic carbons (TOC) in the sediments. The major fractions of PCBs in these sediments are congeners containing 4, 5 or 6 chlorine atoms per biphenyl molecule.     

Nuptial coloration of red shiners (<Emphasis Type="Italic">Cyprinella lutrensis</Emphasis>) is more intense in turbid habitats

Communication is shaped and constrained by the signaling environment. In aquatic habitats, turbidity can reduce both the quantity and quality of ambient light and has been implicated in the breakdown of visual signaling. Here, we examined the relationship between turbidity (quantified with long-term data) and the expression of carotenoid-based nuptial coloration in the red shiner (Cyprinella lutrensis), a small-bodied cyprinid. Males in more turbid habitats displayed redder fins, and an experimental manipulation of adult diet suggested that carotenoid intake alone did not explain among-population color differences. These results run counter to similar studies where signal expression decreased in turbid conditions, and may be explained by the non-territorial red shiner mating system, interactions between the mechanism of coloration and the signaling environment, or reduced cost of color expression in turbid habitats (e.g., reduced predation risk). Our results highlight how the behavioral and ecological contexts in which signals function can shape evolutionary responses to the environment.