Effect of substrate feeding frequencies on the methane production and microbial communities of laboratory-scale anaerobic digestion reactors

Even though full-scale digesters have been designed based on laboratory-scale tests, the substrate feeding modes of laboratory-scale tests might be different from those of full-scale digesters. The effect of substrate feeding frequencies on the performance and microbial community of laboratory-scale anaerobic digestion reactors was investigated. Feeding frequencies of twice a day, once a day, and every two days were tested in three 2-L reactors with an organic loading rate of 0.5 g-glucose/L/day under mesophilic condition. According to the results of this study, all the reactors showed similar methane production rates and SCOD removal efficiencies after sufficient time of acclimation, but frequently feeding promoted more stable digestion. Although there was no significant difference in microbial diversities from pyrosequencing analyses, the changes of archaeal community composition were observed. The decrease in feeding frequency appeared to cause shifts from acetoclastic methanogens affiliated with Methanosaeta to H₂-utilizing methanogens. The increase of Methanosaeta at a frequently feeding might contribute to the stability of reactor operation. Since this study uses glucose as the substrate, there is still possibility of different results for more complex substrates, such as sludge, food waste, etc.     

Are neoliberalist behaviours reflective of bullying? New perspectives on influences on sustainability and global citizenship

The global sustainability crisis facing humanity is a cultural crisis with neoliberal culture, the primary driver. The necessity for global change away from neoliberal systems is well established with cultural systems pursued through sustainability seen as the most viable options to alleviate this global crisis. Whilst the goals of neoliberalism and sustainability are systemic and universal, those implementing them work at a specific level with individuals, groups and/or collectives. The literature fails, however, to provide specific examples of why, on a practical level, social change agents often struggle to implement sustainability goals. One of the primary reasons for these struggles can be found in an examination of human behaviour, for instance personality types, group dynamics and/or interpersonal or group communication skills (or lack thereof). This exploratory paper will investigate the existence of a nexus between neoliberalist and adult bullying behaviours to initiate discussion on the barriers this combination may have on social change for sustainability and global citizenship. This examination is warranted as the propensity in the neoliberalist system to support the use of bullying behaviours by its advocates is a complex, nuanced and underresearched topic. There are implications here for policy development, social and urban planning, education and governance for sustainability and global citizenship.     

Evaluating the impact of green roof evapotranspiration on annual building energy performance

The performance of the building envelope predominantly determines the ultimate energy performance throughout the lifecycle of a building. A sustainable alternative to enhance roof performance while limiting heat flux through a roof is integrating passive techniques such as green roof. Particularly, green roof performance is sensitive to local climate. The main objective of this study was to evaluate the evapotranspiration effect of an extensive green roof on annual energy consumption of an office building in relation to the humid continental climate of Republic of Korea. The dynamic behavior of green roof and building energy performance were investigated through a parametric simulation method using green roof module in EnergyPlus coupled with jEPlus. Structural data of the reference building and ASHARE 90.1-2007 operational schedules were used as inputs for baseline building model while inputs for the green roof module were based on experimental data sets. Due to the influence of the humid conditions and local wind current on the evapotranspiration process, it was generally found that high leaf area index (LAI) reduced cooling energy demand and somewhat reduced heating energy demand as well; corresponding to the highest daily evapotranspiration fluxes of 4.79 mm day^?1 in summer and 1.80 mm day^?1 in winter. Increasing LAI from 20% to 100% cover increased evapotranspiration flux by 10.4% in summer and 80.2% in winter. Thus to minimize energy losses in winter, foliage cover must be carefully considered. Within limitations specified, the overall annual building energy consumption deceased by 90.9 GJ (3.7%).     

Characterization of dried sewage sludge for co-firing in coal power plant by using thermal gravimetric analysis

This research studied the characteristics of dried sewage sludge using TGA to co-fire dried sewage sludge with coal in power plants. The sewage sludges that were discharged from Daejeon, Korea were dried and examined fundamental properties to use them as a fuel. Also, the properties of bituminous coal and wood pellet, which are used in domestic coal power plants, were analyzed and compared with them of sewage sludges and non-isothermal analyses of dried sewage sludges were performed at the heating rates of 5, 10, 20, and 30C /min using TG analyzer to investigate the basic combustion characteristics. As a results of these TGA/DTG analyses, sewage sludges showed its primary peak at the temperature of 250–500?°C, which overlapped with main peak of wood and secondary peak at around 500–600?°C, which overlapped with main peak of coals. Also for the interpretation by Friedman method, the activation energies in the section of highest weight loss were 525.16 kJ/mole for dried digested sewage sludge, 544.88 kJ/mole for dried excess sewage sludge, 203.86 kJ/mole for wood pellet and 146.4585 kJ/mole for bituminous coal. The reaction orders for dried digested excess sewage sludge, dried excess sewage sludge, wood pellet and bituminous coal were 28.775, 24.319, 18.398 and 9.1005, respectively, and the frequency factors were 5.89?\(\times \hspace{0.17em}\)10²⁸, 1.65?\(\times \hspace{0.17em}\)10^24,, 9.59?\(\times \hspace{0.17em}\)10¹⁶ and 1.77?\(\times \hspace{0.17em}\)10⁸ for each, respectively.     

Characterizations of biochar from hydrothermal carbonization of exhausted coffee residue

The aim of this study was to produce renewable energy from exhausted coffee residue, which is a form of biomass. As coffee preference continues to increase, the importation of coffee beans has been increasing sharply. However, the amount of coffee that is actually consumed is only about 0.2% of coffee beans, while the spent coffee beans are discarded in the form of exhausted coffee residue. Hydrothermal carbonization is a method of producing an improved fuel from renewable energy sources by changing the physical and chemical properties of biochars. Biochars were obtained from a variety of reaction temperatures during hydrothermal carbonization and analyzed using elemental analysis, ultimate analysis, and calorific value measurement. The atomic C/O and C/H ratios of all obtained biochars decreased and were found to be similar to those of lignite and sub-bituminous coal. The highest energy recovery efficiency of biochar indicates that the optimum reaction temperature for hydrothermal carbonization was between 210 and 240 °C, which produced biochars with calorific value of approximately 26–27 MJ/kg. The spectra of biochars obtained from Fourier transform infrared spectroscopy (FTIR) showed fewer C–O and aliphatic C–H functional groups, but more carbonyl C=O functional groups and aliphatic CH _x groups. The results of this study indicate that hydrothermal carbonization can be used as an effective means to generate highly energy-efficient renewable fuel resources from coffee residue. The thermogravimetric analysis provided the changing combustion characteristics due to increased fixed carbon content.     

Comprehensive modeling of HFC-23 incineration in a CDM incinerator

Considering that a significant part of used refrigerants have to be destroyed in an environmentally friendly manner together with the high global warming potential (GWP) of HFCs (hydrofluorocarbons), the development of a proper incineration method of HFCs becomes one of the viable methods in the refrigeration and air-conditioning industry. To this end, in this study, the development of a comprehensive modeling of CHF₃ (HFC-23 or R-23) incineration is made to assist in the proper design and determination of optimal operating condition of a practical HFCs incinerator, since the refrigerant of CHF₃ is one of typical HFCs. For this, numerical investigation was performed by the development of a predictive model for the thermal destruction of the CHF₃ using CH₄–air flames in an incinerator designed for CDM (clean development mechanism) project. First of all, comparison between calculation and operation data was made to evaluate the program developed in this study. Numerical calculation of CHF₃–CH₄–air flame predicts successfully the operation data of a CDM incinerator such as temperature, CHF₃ destruction rate more than 99.99 % and other species concentrations such as CO and NO at the exit of the incinerator. Further parametric study was performed also in terms of important variables such as excess air, amount of steam and incinerator size. In general, the results obtained appear physically acceptable and give a clear physical insight into the role of the important variables. Further work is strongly recommended for the development of a general turbulent reaction model for the thermal destruction of HFCs, especially for the condition of non-equilibrium turbulent reaction dominance.     

Sequential production of pyrolytic oil and biodiesel from oil-bearing biomass

Oil extraction from the oil-bearing biomass and waste materials has been considered as one of the biggest challenges in the biodiesel production process because it has been considered as the most energy- and cost-demanding step. This work provides a promising approach for the direct transformation without oil extraction from calcined montmorillonite clay (CMC) and microalgae by means of the non-catalytic thermo-chemical process in conjunction with the real continuous flow system. The introduced method showed the high tolerance of water, impurities, and free fatty acids (FFAs), which enable the combination of the esterification of FFAs and transesterification of triglycerides into a single step without the lipid extraction. For example, this study showed that the maximum achievable yield of biodiesel via the introduced methodology was 97 ± 0.5 % at the temperature regime of 380–480 °C and this biodiesel yield was enhanced in the presence of CO₂. Thus, the introduced methodology for producing biodiesel could be an alternative way of the methanol liquefaction and transesterification under supercritical conditions.     

Sodium bisulfate and a sodium bisulfate/tannin mixture decreases pH when added to an in vitro incubated poultry cecal or fecal contents while reducing Salmonella Typhimurium marker strain survival and altering the microbiome

The objective of the present study was to investigate the ability of animal feed-grade sodium bisulfate (SBS) and a mixture of sodium bisulfate/tannin to inhibit the growth of Salmonella using an anerobic in vitro mixed cecal culture to mimic the conditions within the chicken cecum. An initial inoculum of Salmonella Typhimurium was introduced to an anerobic dilution solution containing 1/3000 diluted cecal bacteria and solids consisting of ground chicken feed and different percentages of solid SBS or SBS/tannin, and surviving organisms were enumerated. Two different experimental designs were employed. In the “unadapted” treatment, the S. Typhimurium was added at the beginning of the culture incubation along with cecal bacteria and chicken feed/SBS or chicken feed/SBS/tannin. In the “adapted” treatment, S. Typhimurium was added after a 24 hour pre-incubation of the cecal bacteria with the chicken feed/SBS or chicken feed/SBS/tannin. Adding SBS resulted in reduction of pH in the cultures which paralleled with the reduction of S. Typhimurium. The SBS alone was found to be inhibitory to S. Typhimurium in the adapted treatment at all concentrations tested (0.25, 0.5, and 0.75%), and the degree of inhibition was concentration-dependent. Salmonella Typhimurium was completely killed in the adapted culture with 0.5% SBS after 24 and 48 h. The SBS/tannin mixture was less inhibitory than SBS alone at the same concentrations in side-by-side comparisons. Testing at a 0.5% SBS concentration, chicken age had little or no effect on log reduction of S. Typhimurium relative to age-matched control cultures without SBS, but age did affect the absolute number of S. Typhimurium surviving, with the greatest decreases occurring at 2 and 4 weeks of age (approx. 10³ S. Typhimurium surviving) compared to 6 weeks of age (approx. 10⁵ Salmonella surviving). Microbiome analysis with an Illumina MiSeq platform was conducted to investigate bacterial compositional changes related to the addition of SBS. The relative abundance of Firmicutes (at the phylum level) was decreased, and genera Lactobacillus and Faecalibacterium were increased when SBS was added to the anaerobic mixed culture containing either fecal or cecal material. The antimicrobial action of feed-grade SBS may represent a potential pre-harvest control measure for Salmonella in poultry production.     

Determining soil quality in urban agricultural regions by soil enzyme-based index

Urban agricultural soils are highly variable, and careful selection of sensitive indicators is needed for the assessment of soil quality. This study is proposed to develop an index based on soil enzyme activities for assessing the quality of urban agricultural soils. Top soils were collected from urban agricultural areas of Korea, and soil chemical properties, texture, microbial fatty acids, and enzyme activities were determined. The soils belonged to five textural classes with the highest frequency of sandy loam. There was no clear correlation between the soil chemical properties and soil microbial properties. Principal component analysis (PCA) and factor analysis were applied to microbial groups for identification of microbial community variation in soils. Two soil groups, namely group 1 (G1) and group 2 (G2), based on microbial community abundance were examined by PCA, and those were more prominent in factor analysis. The G1 soils showed higher microbial community abundance than G2 soils. The canonical discriminant analysis was applied to the enzyme activities of sandy loam soil to develop an index, and the index validation was confirmed using the unused soils and published data. The high-quality soils in published literature assigned the high valued index. Microbial fatty acids and soil enzyme activities can be suitable indicators for soil quality evaluation of urban agricultural soils.