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31.
Surya Kalyani S Jitender Sharma Surender Singh Prem Dureja Lata 《Journal of environmental science and health. Part. B》2013,48(7):663-672
Endosulfan (6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9-methano-2,3,4-benzo-dioxathiepin-3-oxide) is a cyclodiene organochlorine currently used as an insecticide all over the world and its residues are posing a serious environmental threat. This study reports the enrichment and isolation of a microbial culture capable of degrading endosulfan with minimal production of endosulfan sulfate, the toxic metabolite of endosulfan, from tropical acid soil. Enrichment was achieved by using the insecticide as sole sulfur source. The enriched microbial culture, SKL-1, later identified as Pseudomonas aeruginosa, degraded up to 50.25 and 69.77 % of α and β endosulfan, respectively in 20 days. Percentage of bioformation of endosulfan sulfate to total formation was 2.12% by the 20th day of incubation. Degradation of the insecticide was concomitant with bacterial growth reaching up to an optical density of 600 nm (OD600) 2.34 and aryl sulfatase activity of the broth reaching up to 23.93 μg pNP/mL/hr. The results of this study suggest that this novel strain is a valuable source of potent endosulfan–degrading enzymes for use in enzymatic bioremediation. Further, the increase in aryl sulfatase activity of the broth with the increase in degradation of endosulfan suggests the probable involvement of the enzyme in the transformation of endosulfan to its metabolites. 相似文献
32.
Effects of Stocking Rate on the Variability of Peak Standing Crop in a Desert Steppe of Eurasia Grassland 总被引:1,自引:0,他引:1
Zhongwu Wang Shuying Jiao Guodong Han Mengli Zhao Haijun Ding Xinjie Zhang Xiaoliang Wang Eldon L. Ayers Walter D. Willms Kris Havsatad Lata A Yongzhi Liu 《Environmental management》2014,53(2):266-273
Proper grazing management practices can generate corresponding compensatory effects on plant community production, which may reduce inter-annual variability of productivity in some grassland ecosystems. However, it remains unclear how grazing influences plant community attributes and the variability of standing crop. We examined the effects of sheep grazing at four stocking rate treatments [control, 0 sheep ha?1 month?1; light (LG), 0.15 sheep ha?1 month?1; moderate (MG), 0.30 sheep ha?1 month?1; and heavy (HG), 0.45 sheep ha?1 month?1] on standing crop at the community level and partitioned by species and functional groups, in the desert steppe of Inner Mongolia, China. The treatments were arranged in a completely randomized block design over a 9-year period. Standing crop was measured every August from 2004 to 2012. Peak standing crop decreased (P < 0.05) with increasing stocking rate; peak standing crop in the HG treatment decreased 40 % compared to the control. May–July precipitation explained at least 76 % of the variation in peak standing crop. MG and HG treatments resulted in a decrease (P < 0.05) in shrubs, semi-shrubs, and perennials forbs, and an increase (P < 0.05) in perennial bunchgrasses compared to the control. The coefficients of variation at plant functional group and species level in the LG and MG treatments were lower (P < 0.05) than in the control and HG treatments. Peak standing crop variability of the control and HG community were greatest, which suggested that LG and MG have greater ecosystem stability. 相似文献