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171.
Immunotoxicological effects of environmentally relevant concentrations (10, 23, 50, 100 microg/l) of atrazine were studied in Lymnaea stagnalis. Individual hemolymph sampling was performed at 0, 24, 48, 72, 96, 168, 336, 504 and 672 h during exposure. Every atrazine concentration induced a significant increase in the mean number of circulating hemocytes, without any concentration-response relation. A peak (1.6-fold increase) of hemocyte density was observed after 96 h of exposure. After 504 h, the number of hemocytes remained higher only in the snails exposed to the two highest concentrations. Granulocytes contributed most to the increase in hemocyte density in herbicide-exposed snails. Both short- (24 and 96 h) and long-term (504 h) exposures resulted in significant inhibition of hemocyte phagocytic activity upon E. coli. Over the long-term, phagocytosis recovered for the two lowest concentrations. After 504 h of exposure, every herbicide level resulted in a significant reduction of reactive oxygen species production in E. coli-stimulated hemocytes, which was not observed for short-term exposures. 相似文献
172.
Under complementary sex determination (CSD), females of Hymenoptera arise from diploid, fertilized eggs and males from haploid,
unfertilized eggs. Incidentally, fertilized eggs that inherit two identical alleles at the CSD locus will develop into diploid
males. Diploid males are usually unviable or sterile. In a few species, however, they produce diploid sperm and father a triploid
female progeny. Diploid males have been reported in a number of social Hymenoptera, but the occurrence of triploid females
has hardly ever been documented. Here, we report the presence of triploid females, diploid males, and diploid sperm (produced
by diploid males and stored in queen spermathecae) in the ant Tapinoma erraticum. Moreover, we show variations in the frequency of triploids among female castes: Triploid females are more frequent among
workers than virgin queens; they are absent among mated, reproductive queens. The frequency of triploid workers also varies
between populations and between nests within populations. 相似文献
173.
Turbulent velocity profile in fully-developed open channel flows 总被引:2,自引:0,他引:2
Hossein Bonakdari Frédérique Larrarte Laurent Lassabatere Claude Joannis 《Environmental Fluid Mechanics》2008,8(1):1-17
The determination of velocity profile in turbulent narrow open channels is a difficult task due to the significant effects
of the anisotropic turbulence that involve the Prandtl’s second type of secondary flow occurring in the cross section. With
these currents the maximum velocity appears below the free surface that is called dip phenomenon. The well-known logarithmic
law describes the velocity distribution in the inner region of the turbulent boundary layer but it is not adapted to define
the velocity profile in the outer region of narrow channels. This paper relies on an analysis of the Navier–Stokes equations
and yields a new formulation of the vertical velocity profile in the center region of steady, fully developed turbulent flows
in open channels. This formulation is able to predict time averaged primary velocity in the outer region of the turbulent
boundary layer for both narrow and wide open channels. The proposed law is based on the knowledge of the aspect ratio and
involves a parameter CAr depending on the position of the maximum velocity (ξdip). ξdip may be derived, either from measurements or from an empirical equation given in this paper. A wide range of longitudinal
velocity profile data for narrow open channels has been used for validating the model. The agreement between the measured
and the computed velocities is rather good, despite the simplification used. 相似文献
174.
Microscale investigations of the fate of heavy metals associated to iron-bearing particles in a highly polluted stream 总被引:1,自引:0,他引:1
Emmanuelle Montarges-Pelletier Caroline Duriez Jaafar Ghanbaja Laurent Jeanneau Gerard Falkenberg Laurent J. Michot 《Environmental science and pollution research international》2014,21(4):2744-2760
As it flows through a dense steelmaking area, the Fensch River does transport iron-rich particles and colloids, displaying high contents in metallic contaminants (Zn, Cr, Pb, Cu, Ni, and As). Chemical analysis using inductively coupled plasma mass spectrometry (ICP-MS) was carried out on three compartments—waters, suspended materials, and sediments—along the river linear. The variations of metallic trace element concentrations along the river were shown to be partially related to external inputs (industrial and domestic wastewaters and urban surfaces leaching). However, some discrepancies of element partitioning were evidenced. Pb, Cu, and Mn tend to concentrate in suspended particulate and in dissolved fraction, while Cr and As follow the trend of Fe and concentrate within sediments of the most downstream station, just before the junction with Moselle waters. Zn appears strongly associated to iron-rich particles, resulting in a decrease of its concentration in waters for the last station. Along the Fensch linear, the variation of metal partitioning between water and particulate phases is accompanied with strong modifications of the nature and mineralogy of iron-rich particles, as evidenced by microanalyses using electron and X-ray beams. The combination of bulk analyses using ICP-MS and microanalyses applied to the three compartments allowed us to propose a three-step process “settling–weathering–resuspension” to explain Zn partitioning. 相似文献
175.
Oxidative injury and antioxidant genes regulation in cadmium-exposed radicles of six contrasted Medicago truncatula genotypes 总被引:1,自引:0,他引:1
176.
Spatial and Temporal Evaluation of Hydrological Response to Climate and Land Use Change in Three South Dakota Watersheds
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Manashi Paul Mohammad Adnan Rajib Laurent Ahiablame 《Journal of the American Water Resources Association》2017,53(1):69-88
This study analyzed changes in hydrology between two recent decades (1980s and 2010s) with the Soil and Water Assessment Tool (SWAT) in three representative watersheds in South Dakota: Bad River, Skunk Creek, and Upper Big Sioux River watersheds. Two SWAT models were created over two discrete time periods (1981‐1990 and 2005‐2014) for each watershed. National Land Cover Datasets 1992 and 2011 were, respectively, ingested into 1981‐1990 and 2005‐2014 models, along with corresponding weather data, to enable comparison of annual and seasonal runoff, soil water content, evapotranspiration (ET), water yield, and percolation between these two decades. Simulation results based on the calibrated models showed that surface runoff, soil water content, water yield, and percolation increased in all three watersheds. Elevated ET was also apparent, except in Skunk Creek watershed. Differences in annual water balance components appeared to follow changes in land use more closely than variation in precipitation amounts, although seasonal variation in precipitation was reflected in seasonal surface runoff. Subbasin‐scale spatial analyses revealed noticeable increases in water balance components mostly in downstream parts of Bad River and Skunk Creek watersheds, and the western part of Upper Big Sioux River watershed. Results presented in this study provide some insight into recent changes in hydrological processes in South Dakota watersheds. Editor's note: This paper is part of the featured series on SWAT Applications for Emerging Hydrologic and Water Quality Challenges. See the February 2017 issue for the introduction and background to the series. 相似文献
177.
Adeline Jondeau-Cabaton Amélie Soucasse Emilien L. Jamin Nicolas Creusot Marina Grimaldi Isabelle Jouanin Sélim Aït-Aïssa Patrick Balaguer Laurent Debrauwer Daniel Zalko 《Environmental science and pollution research international》2013,20(5):2705-2720
Complex mixtures of contaminants with potential adverse effects on human health and wildlife are found in the environment and in the food chain. These mixtures include numerous anthropogenic compounds of various origins and structures, which may behave as endocrine disruptors. Mixture’s complexity is further enhanced by biotic and abiotic transformations. It is therefore necessary to develop new strategies allowing the identification of the structure of known, as well as unknown, nuclear receptor (NR) ligands present in complex matrices. We explored the possibility to use NR-based affinity columns to characterize the presence of bioactive molecules in environmental complex mixtures. Estrogen receptor α (ERα)-based affinity columns were used to trap and purify estrogenic substances present in surface sediment samples collected in a French river under mixed anthropogenic pressure. We combined biological, biochemical and analytical approaches to characterize the structure of ligands retained on columns and demonstrate the presence of known active molecules such as bisphenol A and octylphenol, but also of unexpected ERα ligands (n-butylparaben, hydroxyl-methyl-benzofuranone). High resolution mass spectrometry results demonstrate that ERα affinity columns can be used for the isolation, purification and identification of known as well as unknown estrogenic contaminants present in complex matrices. 相似文献
178.
Russo J Lefeuvre-Orfila L Lagadic L 《Environmental pollution (Barking, Essex : 1987)》2007,146(2):420-427
Responses of circulating hemocytes were studied in Lymnaea stagnalis exposed to 10, 30, 90, and 270 microg/L fomesafen for 24 and 504 h. Flow cytometry was used to quantify fomesafen-induced production of reactive oxygen species (ROS), phagocytic activity on Escherichia coli, and oxidative burst when hemocytes were challenged by E. coli or phorbol 12-myristate-13-acetate (PMA). Lysosomal membrane damage was assessed, using the neutral-red retention time (NRRT) assay. Exposure to fomesafen for 24 h resulted in increase in ROS levels and decreases in phagocytosis and the oxidative burst in PMA-stimulated hemocytes. After 504 h, intracellular levels of ROS returned to normal, but phagocytosis of E. coli was still inhibited and the associated oxidative burst significantly reduced. After both durations of exposure, decreases of NRRT indicated that lysosome membrane fragility increased with fomesafen concentration. Potential implications for the health and survival of the snails and consequences on populations are discussed. 相似文献
179.
180.
Claudie Doums Camille Ruel Johanna Clémencet Pierre Fédérici Laurent Cournault Serge Aron 《Behavioral ecology and sociobiology》2013,67(12):1983-1993
Under the hymenopteran single-locus complementary sex-determination system, production of diploid males results from homozygosity at the sex-determiner locus. This arises when both parents transmit identical alleles at the locus to the offspring. In species reproducing asexually through thelytokous parthenogenesis, production of diploid males may also occur when the sex locus undergoes recombination and becomes homozygous in the offspring. Diploid males represent a substantial genetic load in hymenopteran populations because they often produce unviable sperm or sire sterile triploid female offspring. In the Mediterranean ant Cataglyphis cursor, the queen and workers can produce female offspring through automictic thelytokous parthenogenesis with central fusion, a mode of parthenogenesis that increases homozygosity. We report, for the first time, the presence of about 39 % of colonies producing adult diploid males (seven colonies out of 18). Overall, 8 % of adult males were diploid (12 diploid males out of the 146 males genotyped). Genotyping workers from the seven colonies producing diploid males showed that three diploid males were sons of queens and produced by thelytoky, six were probably sons of workers also produced by thelytoky and three were non-natal. Furthermore, the mating of a diploid male with two virgin queens in the laboratory led to the production of sterile triploid workers, which shows that diploid males in C. cursor are fertile, mate successfully and produce viable and functional but probably sterile female offspring. Because diploid males originate from thelytokous reproduction, they are only produced during sexual production and hence do not impair colony growth, which could explain why they are not removed at early brood stages. 相似文献