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Roger W. Parker Timothy D. Phillips Leon F. Kubena Leon H. Russell Norman D. Heidelbaugh 《Journal of environmental science and health. Part. B》2013,48(2):77-91
Abstract Penicillic acid and ochratoxin A are environmentally important toxic fungal metabolites (mycotoxins) that are synergistic in combination. The effects of penicillic acid on the pancreatic enzyme, :arboxypeptidase A were investigated in vitro and in vivo. A broad range of inhibition in vitro of the enzyme by PA was demonstrated with a half‐maximal inhibitory concentration equal to 1.1 x 10‐4M PA. Inhibition of carboxypeptidase A was time and temperature dependent, and resulted in decreased conversion of parent ochratoxin A to the non‐toxic metabolite, ochratoxin alpha. Studies in vivo demonstrated a penicillic acid‐dependent inhibition of pancreatic carboxypeptidase A activity in the mouse and the chicken following multiple oral exposure. It is postulated that the mode of toxic interaction of the two mycotoxins may be due, in part, to impaired detoxification of ochratoxin A through peni‐cillic acid depletion of carboxypeptidase A activity. 相似文献
34.
Leon Billings 《Journal of the Air & Waste Management Association (1995)》2013,63(9):840-842
Some of you would have me discuss today the present politics of clean air, some of you would have me discuss in detail the pending amendments to the Clean Air Act, some would have me discuss the failings and failures of the Clean Air Act. I am going to discuss each of these subjects. I want today to put the Clean Air Act and especially it’s 1976 amendments into context. That context is political, it involves the pending amendments and it involves more than 5 years of experience and administrative and judicial interpretation. 相似文献
35.
James W. McFarland M. Leon Hyatt 《Journal of the American Water Resources Association》1973,9(4):755-767
Alternative futures are defined to be mutually consistent alternative combinations of assumed sets of future conditions. Alternative futures are employed in conjunction with the Wollman-Bonem model to project future water use. The model solutions under different alternative futures provide an indication of the direction and relative magnitude of changes in water use, both in quantity and quality, that may result from changes in policy-influenced variables, technology, and other data. The Wollman-Bonem model is employed in this paper as a tool to illustrate the alternative futures concept. The model can best be cast as an economic model. The model solutions are not given as a set of formal projections, but as various possible water-use-over-time curves. This should aid in disspelling the erroneous idea that it is possible to make distant projections of water use as a single curve. Multiple curves suggest that water use is really a function of many variables. 相似文献
36.
R. W. Hill A. Leon Huber E. K. Israelsen J. P. Riley 《Journal of the American Water Resources Association》1972,8(5):909-921
ABSTRACT. As demands upon available water supplies increase, there is an accompanying increase in the need to assess the downstream consequences resulting from changes at specific locations within a hydrologic system. The problem is approached in this study by hybrid computer simulation of the hydrologic system. Modeling concepts are based upon the development of basic relationships which describe the various hydrologic processes. Within a system these relationships are linked by the continuity-of-mass principle. Spatial resolution is achieved by considering the modeled areas as a series of subbasins. The time increment adopted for the model is one month, so that time varying quantities are expressed in terms of mean monthly values. The model is general in nature and is applied to a particular hydrologic system through a programmed verification procedure whereby model coefficients are evaluated for the particular system. In this study the model is applied to the Bear River basin of western Wyoming, southern Idaho, and northern Utah. Comparisons between observed and computed outflow hydrographs show good agreement. The utility of the model is demonstrated by predicting the effects of various possible water resource management alternatives. The verified hybrid computer program can be digitized for application to the digital computer. 相似文献
37.
Allen LH Albrecht SL Colón-Guasp W Covell SA Baker JT Pan D Boote KJ 《Journal of environmental quality》2003,32(6):1978-1991
Methane (CH4) effluxes by paddy-culture rice (Oryza sativa L.) contribute about 16% of the total anthropogenic emissions. Since radiative forcing of CH4 at current atmospheric concentrations is 21 times greater on a per mole basis than that of carbon dioxide (CO2), it is imperative that the impact of global change on rice CH4 emissions be evaluated. Rice (cv. IR72) was planted in sunlit, closed-circulation, controlled-environment chambers in which CH4 efflux densities were measured daily. The CO2 concentration was maintained at either 330 or 660 micromol mol(-1). Air temperatures were controlled to daily maxima and minima of 32/23, 35/26, and 38/29 degrees C at each CO2 treatment. Emissions of CH4 each day were determined during a 4-h period after venting and resealing the chambers at 0800 h. Diurnal CH4 effluxes on 77, 98, and 119 d after planting (DAP) were obtained similarly at 4-h intervals. Emissions over four-plant hills and over flooded bare soil were measured at 53, 63, and 100 DAP. Emissions were negligible before 40 DAP. Thereafter, emissions were observed first in high-CO2, high-temperature treatments and reached a sustained maximum efflux density of about 7 mg m(-2) h(-1) (0.17 g m(-2) d(-1)) near the end of the growing season. Total seasonal CH4 emission was fourfold greater for high-CO2, high-temperature treatments than for the low-CO2, low-temperature treatment, probably due to more root sloughing or exudates, since about sixfold more acetate was found in the soil at 71 DAP. Both rising CO2 and increasing temperatures could lead to a positive feedback on global warming by increasing the emissions of CH4 from rice. 相似文献
38.
Sellek R Jimenez O Aizpurua C Fernandez-Frutos B De Leon P Camacho M Fernandez-Moreira D Ybarra C Carlos Cabria J 《Journal of environmental monitoring : JEM》2008,10(3):362-369
The aim of this study was to develop a specific and highly sensitive method able to detect very low concentrations of Francisella tularensis in soil samples by real-time PCR (qPCR) with SYBR Green I. tul4 gene, which encodes the 17-kDa protein (TUL4) in F. tularensis strains, was amplified using a LightCycler (LC) device. We achieved a detection limit of 0.69 fg of genomic DNA from F. tularensis subp. holarctica live vaccine strain (LVS), corresponding to a value less than 3.4 genome equivalents per reaction. The qPCR was shown to be specific, highly sensitive and reproducible. In addition, we evaluated 2 new methods for recovering bacteria from soil based on 1-step filtration using glass fiber filters and PVDF filters. These filtration methods enabled us to recover F. tularensis efficiently from soil samples. As few as 50 CFU per 0.5 g of soil were detected by qPCR. Capture enzyme-linked immunosorbent assay (cELISA) allowed us to detect and quantify the amount of bacteria recovered from soil by an immunological method. Although qPCR was more sensitive than cELISA, we did not observe substantial differences in the amount of bacteria quantified by both methods. 相似文献
39.
Sperm competition and the level of polyandry in a bushcricket with large nuptial gifts 总被引:4,自引:0,他引:4
Leon?R.?HockhamEmail author Jefferson?A.?Graves Michael?G.?Ritchie 《Behavioral ecology and sociobiology》2004,57(2):149-154
Variation in paternity due to sperm competition or post-copulatory female choice has a major influence on animal mating system evolution and on the levels of genetic variability in natural populations. However, there are relatively few studies comparing the outcome of sperm-competition experiments in the laboratory with natural variation in polyandry among families from the field. In the bushcricket Ephippiger ephippiger, females mate multiply, and the males provide them with a large, nutritious, and probably expensive, donation at mating. We examined paternity in a series of laboratory matings, where females mated with two males, and amongst a series of families collected from a natural population. In the laboratory, paternity was highly bimodally distributed: 24% of families had offspring fathered by the first male to mate, 68% by the second male (in only 8% was paternity shared). In the field, paternity was more mixed: only 27% of families had a single father, 14% had more than two fathers, whilst 59% had two fathers. While unsuccessful matings may contribute to the highly biased paternity in the laboratory, they cannot fully explain the high incidence of complete P2 families. Nonrandom sperm utilisation is therefore likely. Greater sperm mixing in the field probably results from females mating with more males, but the distribution of paternity also reflects an active process of nonrandom sperm utilisation. Confidence of paternity due to last male advantage may be relatively high in this species, and therefore may have facilitated the evolution of the large spermatophore in E. ephippiger.Communicated by D. Gwynne 相似文献
40.
Ai Leon Kazunori Kohyama Kazuyuki Yagi Yusuke Takata Hiroshi Obara 《Mitigation and Adaptation Strategies for Global Change》2017,22(1):85-98
Cultivated wetland rice fields are a source of methane (CH4) emissions. To estimate CH4 emissions and develop policies to reduce such emissions, information on water management at the farm level is crucial. It is known that farmers implement midseason drainage (MD) to increase rice yields and save water. However, little is known about whether MD is carried out in soils where CH4 emissions are high and how part-time status will influence management. The objective of this study is to identify factors that determine MD implementation using a binomial logistic regression model based on a farm-level survey in Japan and to indicate possible changes in estimates of CH4 emissions, accounting for current water management practices. The implementation rates were significantly higher where the soil types were classified as having the potential for high CH4 emissions. Under current water management practices, the duration of MD and the percentage of continuous flooding were 5 to 7 days longer and approximately 7 % higher, respectively, than the values used by the Greenhouse Gas Inventory Office of Japan, which in turn are used to report greenhouse gas emissions to the United Nations Framework Convention on Climate Change. By accounting for current water conditions with the Tier 2 method, this study indicates that national estimates of CH4 emissions from rice straw application areas could be lowered by 12.7 %. These results may contribute to the development of a mitigation policy that will help to further reduce CH4 emissions. 相似文献