The effects of pyrethroid pesticides (deltamethrin, permethrin and cypermethrin) and an organophosphate ester (methidation) on the activities of carp trypsin, alpha-chymotrypsin, carboxypeptidase A and lipase were studied. The enzymes were isolated from the gastrointestinal tract and the effects of the pesticides were investigated during incubation for 5 min. The activity of trypsin was influenced only slightly by the presence of deltamethrin and methidation, whereas permethrin and cypermethrin caused significant inhibition. The pyrethroid pesticides at lower concentrations resulted in a slight activation of alpha-chymotrypsin. Methidation inhibited the alpha-chymotrypsin activity by about 20%. These pesticides modified the lipase activity to a lesser extent; the highest inhibition was measured with cypermethrin. The carboxypeptidase A activity was inhibited by both pyrethroid pesticides and methidation. The results suggest that these pesticides might interact with the active conformation of the studied hydrolytic enzymes, resulting in changes in their activities. 相似文献
This study reports on the development of an evaluation methodology for assessing quantitatively and qualitatively the effectiveness of seminar and mass-mailing contact techniques. These techniques are used to augment traditional health and safety inspections at commercial and industrial premises in the United Kingdom. One of the major findings is that specific aims and objectives for the intervention should be developed prior to the communication exercise. This is crucial for any subsequent measurement of effectiveness, as the intended effect needs to be considered before the communication exercise takes place. Developing more systematic approaches to evaluation is an important step in helping to learn from previous experiences, that is, to build upon the foundations of good practice and avoid repeating mistakes. In time the results of systematic evaluations can be used to inform future policy making, which may help to create more effective risk reduction strategies. 相似文献
Three decades ago, the observation that first trimester fetuses with excess fluid accumulation at the back of the neck were more likely to be aneuploid, gave rise to a new era of prenatal screening. The nuchal translucency (NT) measurement in combination with serum biomarkers and maternal age, resulted in the first trimester combined screening (FTCS) program. The introduction of noninvasive prenatal testing (NIPT) over the past decade has introduced the option for parents to receive highly sensitive and specific screening information for common trisomy from as early as 10 weeks gestation, altering the traditional pathway FTCS pathway. The retention of the 11–13-week NT ultrasound remains important in the detection of structural anomalies; however, the optimal management of pregnancies with a low-risk NIPT result and an isolated increased NT measurement in an era of advanced genomic testing options is a new dilemma for clinicians. For parents, the prolonged period between the initial diagnosis in first trimester, and prognostic information at each successive stage of investigations up to 22–24 weeks, can be emotionally challenging. This article addresses the common questions from parents and clinicians as they navigate the uncertainty of having a fetus diagnosed with an increased NT after a low-risk NIPT result and presents suggested approaches to management. 相似文献
Fatty acid analysis is an alternative dietary investigation tool that complements the more traditional techniques of stomach
content and faecal analysis that are often subject to a wide range of biases. In applying fatty acid analysis to ecosystem
studies, it is important to have an understanding of the effect diet has on the fatty acid profile of the predator. A feeding
experiment, using crustacean and fish as prey for the European cuttlefish Sepia officinalis, was conducted to evaluate the effect of prey fatty acids on the fatty acid profile of this marine predator. Cuttlefish were
fed on a fish diet for the first 29 days, and then changed to a crustacean diet for a further 28 days. Another group of cuttlefish
was fed on a crustacean diet for the first 29 days, and then changed to a fish diet for a further 28 days. An analysis of
the cuttlefish digestive gland showed that the fatty acid profile reflected that of the prey, with cuttlefish on a crustacean
diet being clearly distinguishable from the cuttlefish on a fish diet. Cuttlefish fed on a fish diet for 29 days prior to
the switch in diet were comparatively higher in 16:0, AA, 20:1ω9, DPA6, DHA, 22:4ω6 and DPA3 than those fed on crustaceans.
Cuttlefish fed on a crustacean diet for 29 days prior to the switch in diet were comparatively higher in 17:1ω8, 18:1ω9, 18:2ω6,
18:1ω7, EPA and 20:2ω6 than those fed on fish. Following a change in diet, the fatty acid profile of the cuttlefish digestive
gland reflected that of the new diet within 14 days. The results confirm that the fatty acid profile of the cuttlefish digestive
gland clearly reflects the profile of its recent diet. It also shows that the digestive gland may not be an organ that accumulates
dietary lipids for long-term storage, but rather is an organ where lipids are rapidly being turned over and potentially excreted. 相似文献
Current legislation within Europe aimed at limitingecosystem damage resulting from inputs of atmosphericpollution is based on the critical load concept. Mineralweathering rates are central to the calculation ofcritical loads (acceptable levels) of acid deposition.The authors have undertaken a number of studies whichillustrate the complications and limitations inherent inpredicting mineral weathering rates and the implicationswhich these have for critical loads calculations andmapping. Calculated weathering rates and critical loadsfor two acid-sensitive parent materials (greywackes andgranites) are presented and are used to illustrate theimpact that uncertainty can have on critical loadexceedances. The results have obvious implications forportraying the uncertainties of critical loads to policy makers. 相似文献
In this paper we look behind the notion of sustainable transport and highlight the tensions and contradictions between the different ways of achieving this. The paper examines transportation plans for a major UK city and describes how these develop the idea and practice of sustainable transport and demand management along three quite distinct trajectories. By focusing on both the technological and the institutional dimensions of three different routes to sustainable transport, the analysis explicates the different ways in which the urban transport problem is framed, the different socio-technical logics of demand management embodied in the plans and the differing ways in which institutional relationships are (re)constituted. A key finding is that technological and institutional innovations are inversely related so that radical technological innovation often serves to reinforce existing social relations of power and exclusion. The conclusion drawn is that the choice is not just between a sustainable and an unsustainable transport future, but also between different forms of sustainability. 相似文献
Mercury (Hg) concentrations were measured in 26 Scottish single malt whiskies, and all found to be very low (<10 ng L−1), posing no threat to human health through reasonable levels of consumption. However, a significant south-to-north declining gradient in Hg concentrations was observed reflecting that reported for atmospheric deposition. We speculate that this gradient could be due to a combination of contemporary deposition and the legacy of industrial mercury emissions and deposition over the last 200 years affecting concentrations in local waters used in whisky production. As UK atmospheric emissions of mercury have declined by 90 % since the 1970s, we suggest that whisky being produced today should have even lower Hg concentrations when consumed in 10- to 15-years time. This reduction may be compromised by the remobilisation of contaminants stored in catchment soils being transferred to source waters, but is very unlikely to raise the negligible health risk due to Hg from Scottish single malt whisky consumption.
Coastal environments host plant taxa adapted to a wide range of salinity conditions. Salinity, along with other abiotic variables, constrains the distribution of coastal plants in predictable ways, with relatively few taxa adapted to the most saline conditions. However, few attempts have been made to quantify these relationships to create niche models for coastal plants. Quantification of the effects of salinity, and other abiotic variables, on coastal plants is essential to predict the responses of coastal ecosystems to external drivers such as sea level rise. We constructed niche models for 132 coastal plant taxa in Great Britain based on eight abiotic variables. Paired measurements of vegetation composition and abiotic variables are rare in coastal habitats so four of the variables were defined using community mean values for Ellenberg indicators, i.e. scores assigned according to the typical alkalinity, fertility, moisture availability and salinity of sites where a species occurs. The remaining variables were the canopy height, annual precipitation, and maximum and minimum temperatures. Salinity and moisture indicator scores were significant terms in over 80 % of models, suggesting the distributions of most coastal species are at least partly determined by these variables. When the models were used to predict species occurrence against an independent dataset 64 % of models gave moderate to good predictions of species occurrence. This indicates that most models had successfully captured the key determinants of the niche. The models could potentially be applied to predict changes to habitats and species-dependent ecosystem services in response to rising sea levels. 相似文献