菱镁矿尾矿制备高活性MgO的性能表征

菱镁矿尾矿经预处理后,在一定条件下制备高活性MgO。使用热重-差热分析仪,分析菱镁矿尾矿的分解温度,应用X射线衍射、SEM分析不同煅烧温度、保温时间对MgO晶体结构的影响,并根据Scherrer公式计算MgO晶粒的尺寸。通过电导率仪测定MgO水化电导率,分析不同条件下的MgO活性,探讨煅烧温度、保温时间、颗粒细度与MgO活性之间的关系。结果发现:煅烧温度在1 050℃,保温时间60 min时,菱镁尾矿完全分解,MgO活性最佳;煅烧温度越高,保温时间越长,MgO的活性越差;MgO颗粒细度越小,活性越高。     

林可霉素菌(Streptomyces lincolnensis)利用吡唑酮废水的研究

采用被吡唑酮废液驯化、分离、筛选后的林可霉素菌,并对其在摇瓶上利用吡唑酮废液中的硫酸铵发酵(7 d)生产林可霉素进行了研究。实验结果表明,废液加入培养基体积比都为1∶10,实验1中菌丝代谢和对照比正常,其中还原糖利用最快,在发酵后期为0.24 mg/L,林可霉素起步效价最低为2 100 IU/mL,与对照相比最后发酵效价降低了70 IU/mL;实验2发酵过程pH值偏低,全程为5.86～6.50,氨基氮代谢缓慢为40 mg/100 mL,最后林可霉素效价最低为4 480 IU/mL;实验3中废液在发酵进入48 h中后期的时候补入能促进菌丝体分泌,最后林可霉素为5 180 IU/mL,比对照发酵水平高出8.82%。可见实验3的实验设计有利于林可霉素菌利用吡唑酮废液生产林可霉素,为废物循环利用、变废为宝的可行性作了有意义尝试。     

Oxidation of polycyclic aromatic hydrocarbons by horseradish peroxidase in water containing an organic cosolvent

Polycyclic aromatic hydrocarbons (PAHs) are environmental contaminants that are toxic, mutagenic, and carcinogenic. We investigated the horseradish peroxidase (HRP)-catalyzed oxidation of PAHs in water containing N,N-dimethylformamide. Four PAHs (anthracene, phenanthrene, pyrene, and fluoranthene) were investigated using single-PAH and mixed-PAH systems. The results provide useful information regarding the preferential oxidation of anthracene over other PAHs regardless of the reaction time, enzyme dosage, and hydrogen peroxide concentration. The removal of PAHs was found to be very strongly correlated with the ionization potential (IP), and much greater PAH oxidation was observed at a lower IP. The oxidation of anthracene was specifically pH- and temperature-dependent, with the optimal pH and temperature being 8.0 and 40 °C, respectively. The redox mediators 1-hydroxybenzotriazole and veratryl alcohol promoted the transformation of anthracene by HRP; 9,10-anthraquinone was the main product detected from the anthracene oxidation system. The results of this study not only provide a better understanding of the oxidation of PAHs by utilizing a plant biocatalyst, but also provide a theoretical basis for establishing the HRP-catalyzed treatment of PAH-contaminated wastewater.     

Influence of UV radiation on chlorophyll,and antioxidant enzymes of wetland plants in different types of constructed wetland

A surface- and vertical subsurface-flow-constructed wetland were designed to study the response of chlorophyll and antioxidant enzymes to elevated UV radiation in three types of wetland plants (Canna indica, Phragmites austrail, and Typha augustifolia). Results showed that (1) chlorophyll content of C. indica, P. austrail, and T. augustifolia in the constructed wetland was significantly lower where UV radiation was increased by 10 and 20 % above ambient solar level than in treatment with ambient solar UV radiation (p?C. indica, P. australis, and T. angustifolia by elevated UV radiation of 10 % was higher in vertical subsurface-flow-constructed wetland than in surface-flow-constructed wetland. The sensitivity of MDA, SOD, POD, and CAT activities of C. indica, P. austrail, and T. augustifolia to the elevated UV radiation was lower in surface-flow-constructed wetland than in the vertical subsurface-flow-constructed wetland, which was related to a reduction in UV radiation intensity through the dissolved organic carbon and suspended matter in the water. C. indica had the highest SOD and POD activities, which implied it is more sensitive to enhanced UV radiation. Therefore, different wetland plants had different antioxidant enzymes by elevated UV radiation, which were more sensitive in vertical subsurface-flow-constructed wetland than in surface-flow-constructed wetland.     

Recycling and pollution control of the End of Life Vehicles in China

The quantity of vehicles has increased rapidly in recent years in China; however, the recycling of End of Life Vehicles (ELV) faces several problems and barriers including the collection difficulty and the environmental pollution caused by improper dismantling. In this paper, based on analysis of the basic situation regarding management and recycling of ELV in China, the ELV flow was introduced, and the potential ELV quantity was estimated. The current management regulations and policies were summarized, and dismantling and pollution control technologies for ELV recycling were introduced, too. It was suggested that with the rapid growth of ELV quantities, more regulations will be enacted, and the recycling network will be more effective; the dismantling technology and pollution control measures will be upgraded, and the recycling and remanufacturing industries will be more combined. The government should pay more attention to improve the management and supervision of, and encourage the development of, high level enterprises.     

碱式硫酸铝溶液中SO42-的测定

建立了以铬黑T为指示剂,乙二胺四乙酸二钠反滴定过量BaCl₂的碱式硫酸铝溶液中SO₄^2-的测定方法。实验确定的操作条件为：待测试样稀释后ρ（SO_4^2-）为0.025 ~0.100 g/L,BaCl₂过量率为25%~100%,并且在加入BaCl₂前将试样煮沸,三乙醇胺加入量为0.10 L/L。该法回收率在98.90%~100.72%,相对标准偏差小于1%。测定方法准确,可靠,且操作简便、快速。     

丙烯酸酯类高吸油树脂的合成及其吸附性能

采用悬浮聚合法,以甲基丙烯酸甲酯(MMA)和丙烯酸丁酯(BA)为单体、二乙烯苯(DVB)为交联剂、过氧化苯甲酰(BPO)为引发剂,合成了一种丙烯酸酯类高吸油树脂。实验结果表明,在m(MMA)∶m(BA)=0.5、DVB加入量为0.8%、BPO加入量为1.2%的最佳条件下,树脂对氯仿的吸油率可达30.6 g/g,对氯仿、柴油、甲苯、废机油、花生油、丙酮等有机溶剂和油品的保油率可达90.0%以上。     

Characterization of an efficient catalytic and organic solvent-tolerant azoreductase toward methyl red from Shewanella oneidensis MR-1

The acyl carrier protein (ACP) phosphodiesterase gene (SO 4396) of Shewanella oneidensis MR-1 which was analyzed to have azoreductase activity was heterologously expressed in Escherichia coli. The ACP phosphodiesterase was found to reach maximum enzyme velocity 220.59 U/mg, named azoreductase in this study. The azoreductase had highest specific activity (153.16 U/mg) at pH 6.5, which showed a preference for nicotinamide adenine dinucleotide (NADH) as electron donor. The phylogenetic tree analysis indicated that the azoreductase had preference for NADH and dependence for flavin mononucleotide (FMN). However, the azoreductase from S. oneidensis MR-1 still had high enzyme activity in the absence of FMN. The Mg²⁺ had a positive influence on the enzyme activity with 25 mM concentration, whereas Cr³⁺, Cd²⁺ usually had significantly negative effect on enzyme activity. The purified azoreductase retained nearly 100 % activity after incubating in 30 % dimethyl sulfoxide (DMSO), 30 % acetone, 30 % methanol, 20 % ethanol, 20 % isopropanol, and 10 % propanol.     

Photochemical degradation of ciprofloxacin in UV and UV/H2O2 process: kinetics, parameters, and products

Photochemical degradation of fluoroquinolone ciprofloxacin (CIP) in water by UV and UV/H₂O₂ were investigated. The degradation rate of CIP was affected by pH, H₂O₂ dosage, as well as the presence of other inorganic components. The optimized pH value and H₂O₂ concentration were 7.0 and 5 mM. Carbonate and nitrate both impeded CIP degradation. According to liquid chromatography–tandem mass spectrometry analysis, four and 16 products were identified in UV and UV/H₂O₂ system, respectively. Proposed degradation pathways suggest that reactions including the piperazinyl substituent, quinolone moiety, and cyclopropyl group lead to the photochemical degradation of CIP. Toxicity of products assessed by Vibrio qinghaiensis demonstrated that UV/H₂O₂ process was more capable on controlling the toxicity of intermediates in CIP degradation than UV process.