Occurrence and fate of the phytotoxin juglone in alley soils under black walnut trees

Juglone (5-hydroxy-1,4-napthoquinone) is a chemical released by walnut trees, which can be toxic at various levels to several plant species. A balance among competing source and sink mechanisms and rates will ultimately determine whether juglone is capable of attaining sufficient levels to be allelopathic to intercrops in a walnut tree agroforestry system. In this study, juglone's release, accumulation, and decline in soil are explored using data from soil beneath a black walnut tree (Juglans nigra L) alley cropping system, greenhouse pot studies, and laboratory sorption/degradation studies. Juglone pore water concentrations estimated from extracts of surficial soil from beneath the alley cropping system exceeded the lowest solution culture toxicity levels reported for some plants of 10(-7) M, but did not exceed the inhibition threshold reported for typical intercrops such as maize and soybeans 10(-5) M. Further assessment of the likely persistence of juglone in soils indicated that juglone is both microbially and abiotically degraded, and that it will be particularly short-lived in soils supporting microbial activity. However, walnut seedlings planted in sand-filled pots clearly showed that juglone is released in measurable quantities to the soil's rhizosphere. Therefore, juglone accumulation in low fertility soils is plausible, and may still be worthy of consideration in management of alley agroforestry systems.     

Creating affordable Internet map server applications for regional scale applications

This paper presents an overview and process for developing an Internet Map Server (IMS) application for a local volunteer watershed group using an Internal Internet Map Server (IIMS) strategy. The paper illustrates that modern GIS architectures utilizing an internal Internet map server coupled with a spatial SQL command language allow for rapid development of IMS applications. The implication of this approach means that powerful IMS applications can be rapidly and affordably developed for volunteer organizations that lack significant funds or a full time information technology staff.     

The safety implications of vehicle seat adjustments

INTRODUCTION: The goal of this study was to gather information on the preferred front seat position of vehicle occupants and to determine the impact of variation in seat position on safety during crashes. METHOD: The study evaluated the relationship between seat position and occupant size using the chi-square test and compared the risk of severe injury for small females and large males with regard to forward and rearward seat position using logistic regression. RESULTS: While smaller drivers sat closer to the steering wheel than larger drivers, front passengers of all sizes used similar seat positions. Additionally, the risk of injury was higher for small, unbelted females in rearward seat positions and large males (belted and unbelted) in forward seat positions. CONCLUSIONS: Occupants who adjust their seats to positions that are not consistent with required federal tests are at a greater risk for severe injury in a crash.     

Partitioning of mono- and polycyclic aromatic hydrocarbons in a river sediment adjacent to a former manufactured gas plant site

The equilibrium distributions, between water and coal-tar contaminated sediment, of 16 monocyclic and polycyclic aromatic hydrocarbons were measured and evaluated for consistency with a Raoult's Law-based quantitative relationship. The quantitative relationship calculates the pore water concentration as the product of the aqueous solubility (or for compounds that are solid at room temperature, the aqueous super-cooled liquid solubility) and the mole fraction concentration of the compound within the liquid coal tar. Sediment was collected at five locations at two depths within a 120 m stretch of a river adjacent to a former manufactured gas plant, and all samples contained non-aqueous phase liquid (NAPL) coal tar. Although the amount of coal tar varied between samples by over an order of magnitude, the Raoult's Law-based NAPL-water partition coefficients for each monocyclic or 2- or 3-ring polycyclic aromatic hydrocarbon measured in this study generally varied within a factor of 2 over all sediments.     

Immobilized humic substances as sorbents

A new method was developed for the immobilization of humic substances. Humic acids (HA) immobilized onto different carriers were studied as sorbents for organic and inorganic substances. The sorption isotherms of 4-aminoazobenzene, Crystal Violet, Methylene Green, and flavine mononucleotide on immobilized HA show that pH and salt concentration have a significant effect on the sorption process, largely depending on the properties of polymeric matrix. Humic acids from different sources showed differing sorption capacity for the studied groups of substances.     

Laboratory studies to characterize the efficacy of sand capping a coal tar-contaminated sediment

Placement of a microbial active sand cap on a coal tar-contaminated river sediment has been suggested as a cost effective remediation strategy. This approach assumes that the flux of contaminants from the sediment is sufficiently balanced by oxygen and nutrient fluxes into the sand layer such that microbial activity will reduce contaminant concentrations within the new benthic zone and reduce the contaminant flux to the water column. The dynamics of such a system were evaluated using batch and column studies with microbial communities from tar-contaminated sediment under different aeration and nutrient inputs. In a 30-d batch degradation study on aqueous extracts of coal tar sediment, oxygen and nutrient concentrations were found to be key parameters controlling the degradation rates of polycyclic aromatic hydrocarbons (PAHs). For the five PAHs monitored (naphthalene, fluorene, phenanthrene, anthracene, and pyrene), degradation rates were inversely proportional to molecular size. For the column studies, where three columns were packed with a 20-cm sand layer on the top of a 5 cm of sediment layer, flow was established to sand layers with (1) aerated water, (2) N(2) sparged water, or (3) HgCl(2)-sterilized N(2) sparged water. After steady-state conditions, PAH concentrations in effluents were the lowest in the aerated column, except for pyrene, whose concentration was invariant with all effluents. These laboratory scale studies support that if sufficient aeration can be achieved in the field through either active and passive means, the resulting microbially active sand layer can improve the water quality of the benthic zone and reduce the flux of many, but not all, PAHs to the water column.     

Anniston community health survey: Follow-up and dioxin analyses (ACHS-II)—methods

High serum concentrations of polychlorinated biphenyls (PCBs) have been reported previously among residents of Anniston, Alabama, where a PCB production facility was located in the past. As the second of two cross-sectional studies of these Anniston residents, the Anniston Community Health Survey: Follow-Up and Dioxin Analyses (ACHS-II) will yield repeated measurements to be used to evaluate changes over time in ortho-PCB concentrations and selected health indicators in study participants. Dioxins, non-ortho PCBs, other chemicals, heavy metals, and a variety of additional clinical tests not previously measured in the original ACHS cohort will be examined in ACHS-II. The follow-up study also incorporates a questionnaire with extended sections on diet and occupational history for a more comprehensive assessment of possible exposure sources. Data collection for ACHS-II from 359 eligible participants took place in 2014, 7 to 9 years after ACHS.     

Influence of home characteristics on airborne and dustborne endotoxin and β-D-glucan

The aim of this study was to assess the associations between airborne and dustborne microbial contaminants (endotoxin and β-D-glucan) and estimate the effects of home characteristics on exposure levels of these microbial contaminants. Endotoxin and β-D-glucan concentrations in airborne inhalable particles, airborne PM1 (<1 μm) and vacuumed dust from 184 residential homes were determined using specific Limulus amebocyte assays. Home characteristics were recorded by visual inspection and questionnaires. Linear regression and correlation analyses were performed. Inhalable endotoxin correlated with dust endotoxin (r = 0.34, p < 0.001) and PM1 endotoxin (r = 0.33, p < 0.001). Inhalable β-D-glucan correlated with dust β-D-glucan (r = 0.18, p < 0.01), but not with PM1 β-D-glucan. Significant correlation was also found between PM1 and dust concentrations for endotoxin (r = 0.26, p < 0.001), but not for β-D-glucan. Multivariate regression analyses showed only one significant association between airborne contaminants and environmental characteristics: inhalable β-D-glucan was positively associated with relative humidity with an effect size (change in the dependent variable corresponding to a unit increase in the independent variable) of 2.32 and p < 0.05. In contrast, several associations were found between dust concentrations and environmental characteristics. Dust endotoxin was positively associated with temperature (2.87, p < 0.01) and number of inhabitants (2.76, p < 0.01), whereas dust β-D-glucan was inversely associated with the presence of dogs (-2.24, p < 0.05) and carpet (-3.05, p < 0.01) in the home. In conclusion, dustborne contaminants were more strongly affected by home characteristics than airborne contaminants. Furthermore, even though statistically significant, the correlations between airborne and dustborne contaminants were weak. This indicates that airborne concentrations cannot be reliably predicted based on dustborne concentrations.     

Environmental exposure to POPs and heavy metals in urban children from Dhaka, Bangladesh

Persistent organic pollutants (POPs) and heavy metals are well known environmental pollutants. Even though numerous studies have been carried out to assess human exposures to these compounds, there is still a lack of data on humans from developing countries, especially in underprivileged children. The objective of this study was to assess the exposure to POPs and heavy metals in children from Dhaka, Bangladesh. One specific aim was to investigate whether children working at, or living close to, open waste disposal sites (WDSs) were more heavily exposed than other urban children. In 2008, blood and serum were collected from 73 children aged 7-16 from five neighbourhoods. Some of the children lived and worked at WDSs (N = 31), others lived next to a WDS (N = 17), whereas some children lived far from such sites (N = 25). Blood levels of lead (B-Pb), cadmium (B-Cd), and selenium (B-Se) were determined by ICP-MS for all subjects. The metal levels were high, with B-Pb overall mean 120 μg L(-1) (range 40-220), B-Cd 0.74 μg L(-1) (0.22-4.1), and B-Se 120 μg L(-1) (81-170). There were no marked differences between children from the different neighbourhoods, or between WDS workers and other children. PCB levels were low and with no contrast between neighbourhoods, for CB-153 the overall mean was 7.0 ng g(-1) fat (2.8-51). In contrast, high levels of DDTs were observed in all children, for 4,4'-DDE 1300 ng g(-1) fat (420-4600), and for 4,4'-DDT 326 ng g(-1) fat (44-1400), indicating ongoing exposure. PBDE levels were low, and BDE-209 was quantitated mainly in children working at or living close to WDSs. In conclusion, the high levels of DDTs, lead and cadmium observed in children from Dhaka are of concern. Many children were exposed at levels where health effects have been observed, or at levels without safety margins.     

Who’s in Charge: Role Clarity in a Midwestern Watershed Group

Studies of collaborative watershed groups show that effective leadership is an important factor for success. This research uses data from in-depth interviews and meeting observation to qualitatively examine leadership in a Midwestern collaborative watershed group operating with government funding. One major finding was a lack of role definition for volunteer steering-committee members. Lack of role clarity and decision-making processes led to confusion regarding project management authority among the group, paid project staff members, and agency personnel. Given the important role of government grants for funding projects to protect water quality, this study offers insight into leadership issues that groups with Clean Water Act Section 319 (h) funds may face and suggestions on how to resolve them.