Direct observations of feeding structures and mechanisms in bivalve molluscs using endoscopic examination and video image analysis

A new technique is described for observing the structures and mechanisms of suspension feeding in bivalves using endoscopic examination and video image analysis. This method permits direct in vivo observations of whole, intact structures of relatively undisturbed specimens. No surgical alterations of shell or tissue are required for most species. Pallial organ activity can be recorded for future observations and analysis. Using this technique we examined three bivalve species, each with different degrees of mantle fusion:Mya arenaria L.Mytilus edulis L., andPlacopecten magellanicus (Gmelin). The specimens were collected between April and September 1990 at various locations in Trinity Bay, Newfoundland, Canada. Particle retention by the gill and transport of material to the palps was observed, and velocity of particles moving on the gill was determined. We demonstrate that the endoscope-video-analysis system is an efficient and affordable technique suitable for studies of pallial organ function and mechanisms of feeding.     

REGULATORY WATER QUALITY MONITORING: A SYSTEMS PERSPECTIVE1

ABSTRACT: Regulatory water quality monitoring has evolved to the point where it is a rather complex system encompassing many monitoring purposes and involving many monitoring activities. Lack of a system's perspective of regulatory monitoring hinders the development of effective and efficient monitoring programs to support water quality management. In this paper the regulatory water quality monitoring system is examined in a total systems context. The purposes of regulatory monitoring are reviewed and categorized according to their legal evolution. The activities of regulatory monitoring are categorized and organized into a system which follows the flow of information through the monitoring program. The monitoring purposes and activities are combined to form a monitoring system matrix - a framework within which the total regulatory water quality monitoring system is defined. The matrix, by defining the regulatory monitoring system and clarifying many interactions within the system, provides a basis upon which a more thorough approach to managing, evaluating, and eventually optimizing regulatory monitoring can be developed.     

DEVELOPMENT OF A BACTERIAL TRANSPORT MODEL FOR COARSE SOILS1

ABSTRACT: A bacterial transport model, developed to analyze bacterial translocation in coarse-grained soils, is presented. The complex governing equation is presented first, followed by analyses of each of the major processes influencing bacterial transport. These analyses suggest simplification of the governing equation is feasible when input data on specific processes are limited or unavailable. Model parameters, including bacterial die-off, bacterial distribution, input bacterial concentration, and saturated hydraulic conductivity, were randomly generated using a procedure known to produce either a normal or log-normal distribution of random numbers. Monte Carlo simulations were completed, and the resulting output was used to generate cumulative frequency distributions showing the probability of bacterial transport beyond various soil depths. Results from these simulations indicate that bacteria have a high probability of traveling through coarse-grained soils when low clay content and soil water temperatures limit bacterial retention and die-off.     

Multilevel modeling in traffic safety research: two empirical examples illustrating the consequences of ignoring hierarchies

OBJECTIVES: This commentary illustrates the advantages of multilevel modeling compared to statistical techniques that ignore hierarchies, based on two empirical traffic safety examples. METHODS: The common concept shared by different definitions of multilevel modeling is identified and illustrated. Each definition defines multilevel modeling in its own way but they all refer to hierarchies. Conceptual issues inherently related to hierarchies are then pointed out and illustrated. RESULTS: Broadly speaking there are two important consequences of ignoring a hierarchical structure in the data. The first consequence, underestimation of standard errors, is illustrated with data from an observational study on seatbelt behavior. Two effects that were significant at the 5% level in a single-level model were no longer found to be significant in a two-level model. The single-level model is therefore bound to lead to erroneous conclusions regarding variables that could have an impact on seatbelt use and, ultimately, on increasing the level of traffic safety. The second consequence, related to contextual information, is illustrated with data from a roadside survey on drink driving. Of particular interest is the relationship between Traffic Count, an aggregated level 2 explanatory variable and Odds of drink driving, an individual level 1 dependent variable. CONCLUSIONS: Like every statistical technique, multilevel models should be used with caution and reservation. However, given certain limitations, multilevel modeling is very useful and valuable to traffic safety research.     

Conservation of biodiversity through taxonomy,data publication,and collaborative infrastructures

Taxonomy is the foundation of biodiversity science because it furthers discovery of new species. Globally, there have never been so many people involved in naming species new to science. The number of new marine species described per decade has never been greater. Nevertheless, it is estimated that tens of thousands of marine species, and hundreds of thousands of terrestrial species, are yet to be discovered; many of which may already be in specimen collections. However, naming species is only a first step in documenting knowledge about their biology, biogeography, and ecology. Considering the threats to biodiversity, new knowledge of existing species and discovery of undescribed species and their subsequent study are urgently required. To accelerate this research, we recommend, and cite examples of, more and better communication: use of collaborative online databases; easier access to knowledge and specimens; production of taxonomic revisions and species identification guides; engagement of nonspecialists; and international collaboration. “Data‐sharing” should be abandoned in favor of mandated data publication by the conservation science community. Such a step requires support from peer reviewers, editors, journals, and conservation organizations. Online data publication infrastructures (e.g., Global Biodiversity Information Facility, Ocean Biogeographic Information System) illustrate gaps in biodiversity sampling and may provide common ground for long‐term international collaboration between scientists and conservation organizations.     

The Missoula Valley semivolatile and volatile organic compound study: seasonal average concentrations

The city of Missoula is located in a high mountain valley (elevation 3200 ft.) in western Montana and contains one of the largest populations in the entire Rocky Mountain Region completely enclosed by mountains. During the 2000/2001 Missoula Valley Sampling Program, ambient levels of 61 semivolatile organic compounds (SVOCs) and 54 volatile organic compounds (VOCs) were originally quantified before refining the analytical program to 28 of the most prominent SVOCs and VOCs found in the Missoula Valley airshed. These compounds were measured over 24-hr periods at two locations throughout an entire year. This study provides the first, comprehensive appraisal of the levels of SVOCs and VOCs measured simultaneously throughout all four seasons at two locations in the Missoula Valley, including those levels measured during the 2000 Montana wildfire season. Generally, SVOC levels were comparable between both sides of the Missoula Valley. However, there were nearly double the amount of VOCs measured at the more urban Boyd Park site compared with the rural Frenchtown sampling site, a result of the greater number of automobiles on the eastern side of the Valley. SVOCs and VOCs were measured at their highest levels of the sampling program during the winter. Forest fire smoke samples collected during the summer of 2000 showed significant increases in SVOC phenolic compounds, including phenol, 2-methylphenol, 4-methylphenol, and 2,4-dimethylphenol. Although there were modest increases in some of the other SVOCs and VOCs measured during the fire season, none of the increases were as dramatic as the phenolics.