Influence of CO<Subscript>2</Subscript> on the micro-structural properties of spider dragline silk: X-ray microdiffraction results

The mechanical properties of spider dragline fibres are altered by CO₂ exposure under anaesthetizing conditions during the spinning process. In order to relate these macroscopic changes to a microscopic model, the extrusion of dragline silk was studied by synchrotron radiation microdiffraction. A brief exposure of a female Nephila senigalensis spider to CO₂ results after an incubation time of less than 7 min in the extrusion of a thread (two fibres) swollen with water. The data are interpreted for a model of crystalline -sheet domains containing nanofibrils, which reinforce a network of protein chains. The protein network absorbs water, leaving the nanofibrils unaffected. A continuous flow of CO₂ results in a co-extrusion of a dragline thread and an isotropic silk fraction, which probably has a glycine-rich sequence. Long CO₂ exposure reduces the axial alignment of nanofibrils, presumably due to a partial destruction of the amorphous network.     

Molecular structures and associations of humic substances in the terrestrial environment

Here we show, for the first time, evidence of the primary molecular structures in humic substances (HS), the most abundant naturally occurring organic molecules on Earth, and their associations as mixtures in terrestrial systems. Multi-dimensional nuclear magnetic resonance (NMR) experiments show us that the major molecular structural components in the mixtures operationally defined as HS are aliphatic acids, ethers, esters and alcohols; aromatic lignin derived fragments; polysaccharides and polypeptides. By means of diffusion ordered spectroscopy, distinct diffusion coefficients consistent with relatively low molecular weight molecules were observed for all the components in the mixtures, and saccharides were the largest single class of component present. Liquid chromatography NMR confirmed that HS components can be easily separated and nuclear Overhauser effect (NOE) enhancements support the finding that the components are of relatively low molecular weight <~2,000 Da. The widely recognized properties of HS, i.e., characteristics indicative of crosslinked, macromolecular networks, can now be explained as aggregation of mixtures, most likely instigated by complexation with metal cations.     

Environmental perspectives of pupils: the development of an attitude and behaviour scale

Summary The object of this study was to construct a measurement instrument to assess two different environmental world views of adolescents and, additionally, to address the adolescents' verbal commitment and actual behaviour. Using a multiple-choice questionnaire the instrument was designed to identify and measure the existing factors that underlie concern for ecological/environmental problems and behaviour towards environment/nature in the age group of 10 to 16 year old pupils. Within the traditional tripartite model covering cognitive, affective and behavioural components the study selected items that made up the majority of corresponding subscales from various previous studies. Using factor analysis the configuration of the scale's dimensionality was stated. Based on the responses of approximately 2000 Bavarian pupils, subscales were extracted via factor analysis and, subsequently, addressed to different segments of environmental concern ranging from attitudes to verbal commitment and actual behaviour. Correlation coefficients and the standardized Cronbach's -values were also surveyed. Additionally, a Kruskal-Wallis analysis was applied in order to locate the subscales' influences on sociodemographic variables such as gender and age and self-reported variables such as the pleasure of being a pupil and school performance. The further purpose of the present study is to provide the basis for follow-up comparisons of preferences and values in pupils within other countries.

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Résumé Le but de la présente étude est la réalisation d'un système de mesure pour l'évaluation de différents niveaux de conscience par rapport à la nature et à la protection de l'environnement, ainsi que des comportements actuels ou envisagés. Le système de mesure s'appuie sur un questionnaire à choix multiples pour évaluer les différents facteurs qui déterminent les niveaux de conscience et les comportements correspondants chez les adolescents de 10 à 16 ans. Dans le cadre du modèle à trois niveaux, qui comprend des composantes cognitives, affectives et comportementales, un grand nombre d'items, en partie issus d'études antérieures, ont été sélectionnés pour former les sous-catégories nécessaires. La configuration des catégories a été garantie par une analyse factorielle.Sur la base des réponses d'environ 2000 élèves, des sous-catégories ont été définies, qui vont depuis les positions de départ jusqu'au comportement actuel. En outre, nous avons défini les coefficients de corrélation, ainsi que les valuers Cronback-Alpha, et une analyse des régressions a été effectuée, qui prend en compte les influences, par exemple, des variables sociodémographiques comme le sexe et l'âge, des résultats scolaires ou de la manière dont l'école est perçue par chacun.La présente étude représente la base aussi bien pour des investigations comparatives entre pays, que pour l'évaluation de l'influence d'un enseignement extra-scolaire adopté.

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Dr. Franz Bogner is the senior author to whom correspondence should be addressed. Manfred Wilhelm is based at the Institut für Medizinische Statistik, Technische Universität, Ismaninger Straße 22, D-81675 München, Germany.     

Eine bemerkenswerte Verwachsung von Uranpecherz und Gold aus den Witwatersrand-Konglomeraten

The Science of Nature -     

Disinfection of surfaces by photocatalytic oxidation with titanium dioxide and UVA light

Particularly in microbiological laboratories and areas in intensive medical use, regular and thorough disinfection of surfaces is required in order to reduce the numbers of bacteria and to prevent bacterial transmission. The conventional methods of disinfection with wiping are not effective in the longer term, cannot be standardized, are time- and staff-intensive and use aggressive chemicals. Disinfection with hard ultraviolet C (UVC) light is usually not satisfactory, as the depth of penetration is inadequate and there are occupational medicine risks. Photocatalytic oxidation on surfaces coated with titanium dioxide (TiO2) might offer a possible alternative. In the presence of water and oxygen, highly reactive OH-radicals are generated by TiO2 and mild ultraviolet A (UVA). These radicals are able to destroy bacteria, and may therefore be effective in reducing bacterial contamination. Direct irradiation with UVC however can produce areas of shadow in which bacteria are not inactivated. Using targeted light guidance and a light-guiding sheet (out of a UVA-transmittant, Plexiglas, for example), as in the method described in the present study, bacterial inactivation over the entire area is possible. The effectiveness of the method was demonstrated using bacteria relevant to hygiene such as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus faecium. For these bacteria, a reduction efficiency (RE) more than 6log10 steps in 60 min was observed. Using Candida albicans, a RE of 2log10 steps in 60 min was seen. Light and scanning electron microscopic examinations suggest that the germ destruction achieved takes place through direct damage to cell walls caused by OH-radicals.