On the coadaptation of offspring begging and parental supply—a within-individual approach across life stages

Parental care is often characterized by complex behavioral interactions between offspring soliciting for food and parents providing food. During this interplay both behaviors, offspring begging and parental provisioning, can exert a selective pressure on the expression of the other. It has, therefore, been predicted that traits involved in this interplay may coevolve and ultimately become (genetically) correlated. Such covariation has—at least at the phenotypic level—been found in a number of cross-fostering studies, including evidence from the canary (Serinus canaria), our model species. However, a common challenge for these studies has been to establish a genetic covariation given the difficulty to disentangle the relative contribution of genetic and maternal effects, as the latter may act already before cross-fostering. We addressed this problem by studying within-individual covariation between begging (expressed at the nestling stage) and provisioning (expressed at the adult stage). In addition, we estimated the degree of heritability of these behaviors using parent-offspring regressions, as inheritance forms a prerequisite for any genetic correlation. Both traits showed a low to moderate non-significant heritability, similar to those previously reported in other bird species. However, offspring begging and parental provisioning did not covary at the intra-individual level. Thus, individuals begging intensively as nestlings were not necessarily individuals that provided more food as adults or vice versa. These findings provide important insights for our understanding of coadaptation, suggesting that factors other than genes such as maternal effects may play a role in adjusting offspring begging to the levels of parental provisioning.     

Changes in Nutrient Emissions, Fluxes and Retention in a North-Eastern European Lowland Drainage Basin

Despite dramatic reductions in the 1990s of N and P emissions in the drainage basin, Lake Peipsi/Chudskoe (Estonia/Russia) is still suffering from algal blooms, probably caused by low N:P ratios of the lake water. To quantify the sources and changes of N and P inputs to the lake as a result of economic changes, we modelled emissions, transfer and in-stream retention using a GIS model. The model was calibrated using river monitoring data from the 1985–1989 period, and used to simulate emissions and loads for five future scenarios for 2015–2019. During the 1985–1999 period, diffuse P emissions decreased relatively more than N diffuse emissions, but this was not reflected in the loads to the lake. P loads decreased relatively less than N loads, which caused a decrease in the N:P ratio of the rivers. About 30–45% of diffuse N emissions and only 3–10% of diffuse P emissions reaches the river network. In-stream retention reduces N and P loads to the lake by about 62% and 72%, respectively. Point sources contribute negligibly to the N load to the lake, but form about one-third of the P load. A target/fast development scenario is the most likely scenario for the 2015–2019 period, resulting in higher nutrient loads than in recent years. We conclude that effective load reductions can be achieved by focussing on diffuse N and P emissions close ( < 50 km²) to the lake and by upgrading P removal capacity in wastewater treatment plants of towns.     

Body feathers as a potential new biomonitoring tool in raptors: a study on organohalogenated contaminants in different feather types and preen oil of West Greenland white-tailed eagles (Haliaeetus albicilla)

We investigated the variation in concentrations and profiles of various classes of organohalogenated compounds (OHCs) in different feather types, muscle tissue and preen oil from 15 white-tailed eagle (Haliaeetus albicilla) carcasses from Greenland. The influence of moult patterns and potential external contamination onto the feather surface was examined, while the present study is also the first to investigate the use of body feathers for OHC monitoring. Concentrations of sum polychlorinated biphenyls (PCBs) in feathers from white tailed eagles ranged from 2.3 ng/g in a primary wing feather to 4200 ng/g in body feathers. Using 300 mg of body feathers, almost 50 different OHCs could be quantified and median concentrations in body feathers were 10 fold higher than concentrations in tail feathers (rectrices) or primary wing feathers. Body feathers could be very useful for biomonitoring taking into account their easy sampling, short preparation time and high levels of OHCs. In addition, the effects of confounding variables such as feather size, moult and age are also minimised using body feathers. Correlations with concentrations in muscle tissue and preen oil were high and significant for all feather types (r ranging from 0.81 to 0.87 for sum PCBs). Significant differences in concentrations and profiles of OHCs were found between different primary feathers, indicating that the accumulation of OHCs in feathers varies over the moulting period (maximum three years). Washing of feathers with an organic solvent (acetone) resulted in a significant decrease in the measured concentrations of OHCs in feathers. However, our results indicated that preen oil is probably not the only contributor to the external contamination that can be removed by washing with acetone. Possibly dust and other particles may be of importance and may be sticking to the preened feathers. Rectrices washed only with water showed high and significant correlations with concentrations in muscle and preen oil as well. Washing with acetone therefore does not seem to be of great influence when relating to internal tissue concentrations. We recommend washing feathers only with distilled water in order to remove dirt and dust particles before analysis.     

Assessing spatial patterns of metal bioaccumulation in French mosses by means of an exposure index

Background, aim and scope  

The European Heavy Metals in Mosses Surveys (UNECE-ICP Vegetation) is a programme performed every 5 years since 1990 in at least 21 European countries. The moss surveys aim at uncovering the spatiotemporal patterns of metal and nitrogen bioaccumulation in mosses. In France, the moss survey was conducted for the third time in 2006. Five hundred thirty-six monitoring sites were sampled across the whole French territory. The aim of the presented study is to give an integrative picture of the metal bioaccumulation for the entire French territory without geographical gaps. Furthermore, confounding factors of the metal bioaccumulation in mosses should be investigated.     

The change of European landscapes: human-nature relationships, public attitudes towards rewilding, and the implications for landscape management in Switzerland

The rewilding of landscapes is one of the most important and intensively discussed landscape changes occurring in Switzerland, as the need for agricultural and forest land is decreasing. To ensure that decisions concerning future landscape management will be supported by the public, it is crucial to take public opinion into account. Hence the present study aims to assess the public attitudes towards nature and "rewilding" processes. In order to analyze these attitudes, we sent a standardized questionnaire to 4000 randomly selected households throughout Switzerland. A cluster analysis led to a typology with four different types of human-nature relationship ("nature lovers", "nature sympathizers", "nature-connected users" and "nature controllers") that each characterize a particular attitude towards nature. These human-nature relationship types differ in their attitudes towards rewilding as well, allowing a rough classification of the sample into wilderness opponents (51.1%) and wilderness proponents (49.9%). However both groups agree with regard to their opinion concerning the rules and regulations that should apply in future wilderness areas. The parallels of the human-nature relationship typology of this survey with other typologies, and the implications for further research are discussed. We can conclude that, due to the differences concerning the attitudes towards wilderness between the human-nature relationship types, between the rural and urban dwellers, and between the language regions, a uniform strategy for the designation and management of wilderness areas in Switzerland is not possible. We recommend that, when managing landscape change, all stakeholders are included in a participatory process and we advise a thorough assessment of the attitudes of the involved persons towards nature and rewilding at the start of such processes. Such an assessment would facilitate the identification of well-defined target groups allowing specific interventions and management actions customized to the needs and characteristics of each of these groups. In addition we see the commonalities between wilderness opponents and wilderness proponents concerning the rules in wilderness areas as an ideal starting point for a successful participatory process.     

Seeds’ physicochemical traits and mucilage protection against aluminum effect during germination and root elongation as important factors in a biofuel seed crop (Ricinus communis)

We determined the length, volume, dry biomass, and density in seeds of five castor bean cultivars and verified notable physicochemical trait differences. Seeds were then subjected to different toxic aluminum (Al) concentrations to evaluate germination, relative root elongation, and the role of root apices’ rhizosphere mucilage layer. Seeds’ physicochemical traits were associated with Al toxicity responses, and the absence of Al in cotyledons near to the embryo was revealed by Al-hematoxylin staining, indicating that Al did not induce significant germination reduction rates between cultivars. However, in the more sensitive cultivar, Al was found around the embryo, contributing to subsequent growth inhibition. After this, to investigate the role of mucilage in Al tolerance, an assay was conducted using NH₄Cl to remove root mucilage before or after exposure to different Al concentrations. Sequentially, the roots were stained with hematoxylin and a quantitative analysis of staining intensity was obtained. These results revealed the significant contribution of the mucilage layer to Al toxicity responses in castor bean seedlings. Root growth elongation under Al toxicity confirmed the role of the mucilage layer, which jointly indicated the differential Al tolerance between cultivars and an efficient Al-exclusion mechanism in the tolerant cultivar.     

First Europe-wide correlation analysis identifying factors best explaining the total nitrogen concentration in mosses

In this study, the indicative value of mosses as biomonitors of atmospheric nitrogen (N) depositions and air concentrations on the one hand and site-specific and regional factors which explain best the total N concentration in mosses on the other hand were investigated for the first time at a European scale using correlation analyses. The analyses included data from mosses collected from 2781 sites across Europe within the framework of the European moss survey 2005/6, which was coordinated by the International Cooperative Programme on Effects of Air Pollution on Natural Vegetation and Crops (ICP Vegetation). Modelled atmospheric N deposition and air concentration data were calculated using the Unified EMEP Model of the European Monitoring and Evaluation Programme (EMEP) of the Convention on Long-range Transboundary Air Pollution (CLRTAP). The modelled deposition and concentration data encompass various N compounds. In order to assess the correlations between moss tissue total N concentrations and the chosen predictors, Spearman rank correlation analysis and Classification and Regression Trees (CART) were applied. The Spearman rank correlation analysis showed that the total N concentration in mosses and modelled N depositions and air concentrations are significantly correlated (0.53 ≤ r_s ≤ 0.68, p < 0.001). Correlations with other predictors were lower than 0.55. The CART analysis indicated that the variation in the total N concentration in mosses was best explained by the variation in NH₄⁺ concentrations in air, followed by NO₂ concentrations in air, sampled moss species and total dry N deposition. The total N concentrations in mosses mirror land use-related atmospheric concentrations and depositions of N across Europe. In addition to already proven associations to measured N deposition on a local scale the study at hand gives a scientific prove on the association of N concentration in mosses and modelled deposition at the European scale.     

Social foraging in stingless bees: how colonies of Melipona fasciata choose among nectar sources

In an experimental set-up, a colony of the stingless bee Melipona fasciata demonstrated its ability to choose the better of two nectar sources. This colony pattern was a result of the following individual behavioural decisions: continue foraging, abandon the feeder, restart foraging and initiate foraging. Only very rarely did individuals switch from one feeder to the other. With the first combination of a rich (2.7 M) and a poor (0.8 M) feeder M. fasciata behaved differently from Apis mellifera. Recruitment occurred to both feeders and the poor feeder was not abandoned completely. When the poor feeder was set to 0.4 M, M. fasciata abandoned the poor feeder rapidly and allocated more foragers to the rich feeder. These patterns were similar to those reported for A. mellifera with the first combination of feeders. Over a sequence of 4 days, experienced bees increasingly determined the colony patterns, and the major function of communication between workers became the reactivation of experienced foragers. The foragers modulated their behaviour not only according to the profitability of the feeder, but also according to previous experience with profitability switches. Thus, experience and communication together regulated colony foraging behaviour. These findings and the results of studies with honeybees suggest that M. fasciata and honeybees use similar decision-making mechanisms and only partly different tools. Received: 21 December 1998 / Accepted: 5 January 1999     

An exposure study with polybrominated diphenyl ethers (PBDEs) in female European starlings (Sturnus vulgaris): toxicokinetics and reproductive effects

We exposed female European starlings to a pentabromodiphenyl ether (Penta-BDE) mixture through subcutaneous implants, and examined levels and profiles of polybrominated diphenyl ethers (PBDEs) together with reproductive effects. Sum PBDE levels increased significantly in the serum of the exposed females from 218 ± 43 to 23,400 ± 2035 pg/ml. Sum PBDE concentrations in the eggs of the exposed group ranged from 130 ± 12 to 220 ± 37 ng/g wet weight (ww). The profile in serum after egg laying was very similar to that in eggs. There were no detectable levels of HO-PBDEs in both serum and eggs. Fewer females of the exposed group initiated egg laying compared to the control group, although the difference was not significant. In addition, egg weight and volume were significantly higher in the exposed group. These results suggest that, at the investigated exposure levels (150 μg sum PBDEs/bird), PBDEs may have a negative effect on reproductive performance.