The loading effect in central place foraging wheatears (Oenanthe oenanthe L.)

Summary The behaviour of five adult wheatears (Oe. oenanthe) delivering prey (maggots and mealworms) from artificial patches to the nestlings was studied. The existence of a so-called loading effect was confirmed. Handling times for subsequent prey items increased with the number held in the beak, which led to a decrease in collecting rates with patch time and, in most cases, to positively accelerating loading functions when collecting times for prey items are plotted as a function of load size. All birds seemed to become more efficient at loading prey in the experimental patches during the course of the nestling period. Loading functions for maggots and mealworms were slightly different. When forcing the birds to visit several cups (only one item in each cup) and remove a layer of moss before reaching the prey (low-density patches), all birds took fewer prey and two of them stayed longer in them than in highdensity patches (one cup filled with prey items). This was due to an increase in search times with the number of prey held in the beak.From knowledge of the loading functions and travel times to the nest, it is possible to predict the optimal load sizes according to a mathematical solution of the delivery rate model of Orians and Pearson (1979). By transforming collecting and travel times to energy expenditures, it is also possible to derive predictions from an energy efficiency model (maximizing energy delivery per unit energy expended in a round-trip). The observed average load sizes did not differ significantly from those predicted by the delivery rate model, but they were significantly smaller in all cases than those predicted by the energy efficiency model. For birds feeding nestlings, it may be more important to sacrifice efficiency in energy expenditure in favour of greater delivery rates, thereby maximizing the growth rate of the young.     

Comparative toxic effects of formulated simazine on Vibrio fischeri and gilthead seabream (Sparus aurata L.) larvae

The use of Early Life Stage (ELS) tests is a useful tool in risk assessment. The purpose of this study was to compare the sensitivity of the seabream (Sparus aurata) larvae with the extensively used Microtox^® test on a commercial formulation containing simazine, an s-triazine herbicide. To this end, survival, growth and histopathological changes displayed by seabream yolk sac larvae exposed during 72 h post-hatching to nominal concentrations of the commercial preparation up to its saturating concentration in water, and bioluminescence of the marine bacteria Vibrio fischeri (Microtox^®) were studied. Survival of larvae after three days of exposure was significantly reduced in the highest (4.5 mg/l) concentration, but no effects on growth were found in any of the simazine treatments. The 72 h LC50 value for yolk sac larvae was estimated as 4.19 mg/l. Commercial grade simazine did not exert any significant toxicity to the marine bacterium V. fischeri at the concentrations tested.     

Land-based sources of marine pollution: organochlorine pesticides in stream systems

BACKGROUND: Organochlorine pesticides (OCPs) have been dispersed ubiquitously in the environment. Bottom sediments act as sinks for these compounds and their concentrations often reflect the degree of anthropogenic pollution. This study was designed to evaluate the occurrence and distribution of OCPs in superficial streambed sediments and their relation to land use in two creeks that contribute to the coastal pollution of the southeastern region of Argentina. METHODS: Sampling sites were selected by a combination of land use and stream type. Las Brusquitas creek, which passes through vast agricultural areas, and La Tapera creek which originates in a natural wetland and passes through horticultural farming and urban areas. OCPs quantification was carried out by GC-ECD. RESULTS AND DISCUSSION: Results showed similar total OCP concentrations in sediments from both creeks in the range of 6-25 ng/g dry wt. However, when OCPs were expressed in ng/g total organic carbon (TOC), La Tapera creek presented 4-fold higher total levels as a consequence of a higher OCP input during the recent past in that watershed. La Tapera outfall showed 4-fold higher levels than that seen in Las Brusquitas, although both values were below the sediment quality criteria demanded to protect wildlife. Sigmaendosulfans, sigmaDDTs and sigmachlordanes were the main OCP group in all samples, with Endosulfan sulfate being the most frequent and abundant compound. The predominance of metabolites with respect to parent compounds suggests a contamination mainly by runoff from aged and weathered agricultural soils. CONCLUSIONS: Despite OCPs being banned, they still exist in creek sediments from the studied region, representing continuous contributions of land-based source contaminants to the marine environment. OUTLOOK: Future research on OCP levels in suspended sediments is recommended in order to determine the total OCP concentrations in the selected stream systems.     

Effects of Spatial and Temporal Data Aggregation on the Performance of the Multi‐Radar Multi‐Sensor System

The objectives of this study were to (1) evaluate the performance of the Multi‐Radar Multi‐Sensor (MRMS) system in capturing precipitation compared to gauge data, and (2) assess the effects of spatial (1–50 km) and temporal (15–120 min) data aggregation scales on the performance of the MRMS system. Point‐to‐grid comparisons were conducted between 215 rain gauges and the MRMS system. The MRMS system at 1 km spatial and 15 min temporal resolutions captured precipitation reasonably well with average R², root mean square error (RMSE), and percent bias (PBIAS) values of 0.65, 0.5 mm, and 11.9 mm; whereas Threat Score, probability of detection, and false alarm ratio were 0.57, 0.92, and 0.40, respectively. Decreasing temporal resolution from 15 min to two hours resulted in an increase in R² and a decrease in RMSE, whereas PBIAS was not affected. Reducing spatial resolution from 1 to 50 km resulted in increases in R² and PBIAS, whereas RMSE was decreased. Increasing spatial aggregation scale from 1 to 50 km resulted in an R² increase of only 0.08. Similarly, improvement in R² was only modest (0.17) compared to an eightfold reduction in temporal resolution (from 15 min to two hours). While aggregating data at coarser temporal resolutions resolved some of the under/overestimation issues of the MRMS system, it was apparent even at coarser spatial and temporal resolutions the MRMS system inherently overestimated smaller precipitation events while underestimated bigger events.     

Benefits of systems thinking for a human and organizational factors approach to safety management

This paper highlights the value of systems theory and its application to human and organizational factors (HOF). HOF specialists consider multiple systems characteristics in their analyses but are often unaware of the relevant theory applied in their analysis. We argue that a structured effort to take key systems characteristics into account in HOF practice would increase the depth and breadth of safety management analyses and help HOF specialists to act more effectively on industrial socio-technical systems. First, the paper identifies the following seven system theory characteristics: constitution, multi-axis representation, limit, emergence, variety, coherence, and causal interaction, which are then illustrated with examples from the HOF field. Finally, we discuss the two main benefits of integrating system thinking in a HOF approach to safety management: (1) an improved understanding of the inner workings of an industrial socio-technical system; and (2) a compendium or a reference to guide for decision-making and the implementation of actions within the industrial socio-technical systems.     

Nest ectoparasites increase physiological stress in breeding birds: an experiment

Parasites are undoubtedly a biotic factor that produces stress. Heat shock proteins (HSPs) are important molecules buffering cellular damage under adverse conditions. During the breeding season, blue tit Cyanistes caeruleus (L.) adults are affected by blood parasites, nest-dwelling parasites and biting flies, potentially affecting their HSP-mediated responses. Here, we treated females with primaquine to reduce blood parasites and fumigated nests with permethrin to reduce nest-dwelling parasites to test whether these treatments affect HSP60 level during the breeding season. Medicated females, but not controls, had a significant reduction of the intensity of infection by Haemoproteus spp. blood parasites. However, final intensity of infection did not differ significantly between groups, and we did not find an effect of medication on change in HSP60 level. Fumigation reduced the abundance of nest-dwelling parasites (mites, fleas and blowfly larvae) and engorged biting midges in nests. Females breeding in non-fumigated nests increased HSP60 levels during the season more than those breeding in fumigated nests. Furthermore, the change in HSP60 level was positively correlated with the abundance of biting midges. These results show how infections by nest ectoparasites during the breeding period can increase the level of HSPs and suggest that biting midges impose physiological costs on breeding female blue tits. Although plausible, the alternative that biting midges prefer to feed on more stressed birds is poorly supported by previous studies.     

Effects of subchronic exposure to carbofuran on antioxidant defence system and malondialdehyde levels in common carp (Cyprinus carpio L.)

The present study focused on the assessment of oxidative stress induction by pesticides such as carbamates which are widely used as insecticides and nematicides and contaminate aquatic ecosystems on certain biomarkers in liver of common carp (Cyprinus carpio L.). Biomarkers selected for stress monitoring were malondialdehyde (MDA), an index of lipid peroxidation, and antioxidant defence system enzymes, mainly catalase (CAT), glutathione reductase (GR), and glutathione-S-transferase (GST) activities in liver of fish exposed to 0, 10, 50, or 100?µg?L^?1 of carbofuran for 4, 15, or 30 days. Oxidative stress was found in liver of common carp exposed to carbofuran which was manifested by a decrease in CAT and GR activities after 4 and 30 days of exposure. An adaptive response was probably produced since at day 15 no modifications in the CAT activity and increased GR activity were observed. In addition, a decrease in MDA content with the highest concentration of carbofuran used was found after 30 days of exposure. However, no significant changes were found in GST activity showing a varied response. The results concerning oxidative and antioxidant profiles indicate that subchronic exposure to the insecticide carbofuran is capable of inducing oxidative stress in fish.     

A global evaluation of metabolic theory as an explanation for terrestrial species richness gradients

We compiled 46 broadscale data sets of species richness for a wide range of terrestrial plant, invertebrate, and ectothermic vertebrate groups in all parts of the world to test the ability of metabolic theory to account for observed diversity gradients. The theory makes two related predictions: (1) In-transformed richness is linearly associated with a linear, inverse transformation of annual temperature, and (2) the slope of the relationship is near -0.65. Of the 46 data sets, 14 had no significant relationship; of the remaining 32, nine were linear, meeting prediction 1. Model I (ordinary least squares, OLS) and model II (reduced major axis, RMA) regressions then tested the linear slopes against prediction 2. In the 23 data sets having nonlinear relationships between richness and temperature, split-line regression divided the data into linear components, and regressions were done on each component to test prediction 2 for subsets of the data. Of the 46 data sets analyzed in their entirety using OLS regression, one was consistent with metabolic theory (meeting both predictions), and one was possibly consistent. Using RMA regression, no data sets were consistent. Of 67 analyses of prediction 2 using OLS regression on all linear data sets and subsets, two were consistent with the prediction, and four were possibly consistent. Using RMA regression, one was consistent (albeit weakly), and four were possibly consistent. We also found that the relationship between richness and temperature is both taxonomically and geographically conditional, and there is no evidence for a universal response of diversity to temperature. Meta-analyses confirmed significant heterogeneity in slopes among data sets, and the combined slopes across studies were significantly lower than the range of slopes predicted by metabolic theory based on both OLS and RMA regressions. We conclude that metabolic theory, as currently formulated, is a poor predictor of observed diversity gradients in most terrestrial systems.     

Physicochemical variations in atmospheric aerosols recorded at sea onboard the Atlantic–Mediterranean 2008 Scholar Ship cruise (Part II): Natural versus anthropogenic influences revealed by PM10 trace element geochemistry

The geochemistry of PM₁₀ filter samples collected at sea during the Scholar Ship Atlantic–Mediterranean 2008 research cruise reveals a constantly changing compositional mix of pollutants into the marine atmosphere. Source apportionment modelling using Positive Matrix Factorization identifies North African desert dust, sea spray, secondary inorganic aerosols, metalliferous carbon, and V–Ni-bearing combustion particles as the main PM₁₀ factors/sources. The least contaminated samples show an upper continental crust composition (UCC)-normalised geochemistry influenced by seawater chemistry, with marked depletions in Rb, Th and the lighter lanthanoid elements, whereas the arrival of desert dust intrusions imposes a more upper crustal signature enriched in “geological” elements such as Si, Al, Ti, Rb, Li and Sc. Superimposed on these natural background aerosol loadings are anthropogenic metal aerosols (e.g. Cu, Zn, Pb, V, and Mn) which allow identification of pollution sources such as fossil fuel combustion, biomass burning, metalliferous industries, and urban–industrial ports. A particularly sensitive tracer is La/Ce, which rises in response to contamination from coastal FCC oil refineries. The Scholar Ship database allows us to recognise seaborne pollution sourced from NW Africa, the Cape Verde and Canary islands, and European cities and industrial complexes, plumes which in extreme cases can produce a downwind deterioration in marine air quality comparable to that seen in many cities, and can persist hundreds of kilometres from land.     

Alterations observed in the endothelial HUVEC cell line exposed to pure Cylindrospermopsin

The cyanobacterial toxin Cylindrospermopsin (CYN) is receiving great interest due to its increasing presence in waterbodies, which has lead to recognize it as a potential threat to drinking water safety. CYN is a potent inhibitor of protein and glutathione synthesis. The present work studies for the first time the effects of CYN in endothelial cells. The basal cytotoxicity endpoints studied at 24 and 48 h were total protein content (PC), neutral red (NR) uptake and the tretazolium salt, MTS, reduction. Moreover, the effect of subcytotoxic concentrations of CYN on the generation of intracellular reactive oxygen species (ROS), the activity of γ-glutamylcysteine synthetase (GCS) and glutathione (GSH) content have been investigated. In addition, morphological alterations of HUVEC cells subsequent to CYN exposure were recorded.The cytotoxicity endpoints revealed a decrease in the cellular viability in a time and concentration-dependent way. The most sensitive cytotoxicity endpoint was NR uptake assay, with reductions in cell viability of 95% at 48 h of exposure to 40 μg mL⁻¹ CYN. Intracellular ROS production was increased only at the lowest concentration assayed, while GCS activity and GSH content underwent concentration-dependent enhancements. The most remarkable morphological alterations observed were: nucleolar segregation with altered nuclei, degenerated Golgi apparatus, increases in the presence of granules and apoptosis.