Modelling the outcrossing between genetically modified and conventional maize with equation discovery

Many studies explore the feasibility of co-existence between genetically modified (GM) and conventional (non-GM) crops. An important research topic in these studies is the process of outcrossing, i.e., the process of gene flow via pollen flow from GM to non-GM crops. In this paper, we address a new modelling approach to define the environmentally driven processes of outcrossing for maize from existing empirical datasets. In particular, we use equation discovery methodology that combines background knowledge and empirical data from several studies. We induce models that predict the degree of outcrossing rate between the donor (GM) and the recipient (non-GM) maize field from the distance between the fields and the local wind characteristics (speed, direction and duration). This results in highly accurate models, for which both variables (distance and wind) are essential and of roughly equal importance.     

Genotoxic potential of selected cytostatic drugs in human and zebrafish cells

Due to their increasing use, the residues of anti-neoplastic drugs have become emerging pollutants in aquatic environments. Most of them directly or indirectly interfere with the cell’s genome, which classifies them into a group of particularly dangerous compounds. The aim of the present study was to conduct a comparative in vitro toxicological characterisation of three commonly used cytostatics with different mechanisms of action (5-fluorouracil [5-FU], cisplatin [CDDP] and etoposide [ET]) towards zebrafish liver (ZFL) cell line, human hepatoma (HepG2) cells and human peripheral blood lymphocytes (HPBLs). Cytotoxicity was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and acridine orange/ethidium bromide staining. All three drugs induced time- and dose-dependent decreases in cell viability. The sensitivity of ZFL and HepG2 cells towards the cytotoxicity of 5-FU was comparable (half maximal inhibitory concentration (IC₅₀) 5.3 to 10.4 μg/mL). ZFL cells were more sensitive towards ET- (IC₅₀ 0.4 μg/mL) and HepG2 towards CDDP- (IC₅₀ 1.4 μg/mL) induced cytotoxicity. Genotoxicity was determined by comet assay and cytokinesis block micronucleus (CBMN) assay. ZFL cells were the most sensitive, and HPBLs were the least sensitive. In ZFL cells, induction of DNA strand breaks was a more sensitive genotoxicity endpoint than micronuclei (MNi) induction; the lowest effective concentration (LOEC) for DNA strand break induction was 0.001 μg/mL for ET, 0.01 μg/mL for 5-FU and 0.1 μg/mL for CDDP. In HepG2 cells, MNi induction was a more sensitive genotoxicity endpoint. The LOEC values were 0.01 μg/mL for ET, 0.1 μg/mL for 5-FU and 1 μg/mL for CDDP. The higher sensitivity of ZFL cells to cytostatic drugs raises the question of the impact of such compounds in aquatic ecosystem. Since little is known on the effect of such drugs on aquatic organisms, our results demonstrate that ZFL cells provide a relevant and sensitive tool to screen genotoxic potential of environmental pollutant in the frame of hazard assessment.     

Mathematical modelling of pulsed magnetic field action on neurons’ bioelectric activity

Hodgkin–Huxley model, describing generation of action potentials in axons’ membranes, has been used for appreciation of the possible mechanisms of electromagnetic fields influence on neurons’ electrical activity. It follows from the carried calculations that flowing through an axon of the external current pulses, which have an area of rise, an area of DC, and an area of drop, does not influence own excitation pulses passage if periods of rise and drop are slope enough. Depending on the external current parameters, own excitation pulses may not be influenced or may be suppressed at greater velocity of rise and drop of an external current. Moreover, induced excitation pulses appear at definite parameters of an external current. In the case, when at passage through an axon of an external current, own excitation pulses follow one by one (paired pulse facilitation), and the second pulse may be suppressed if its level will not be increased additionally in comparison with the case of an external current absence. It is possible that such increase happens “automatically” as a result of “tuning” (adaptation) of axons to the conditions of flowing through them of an external current. It may be an explanation of the phenomena observed in experiments.     

波罗的海地区地方级可持续政策:波罗的海城市联盟网中的地方21世纪议程

"地方21世纪议程"(LA21)有两个主要目标.①在本研究论文中的一些经验证据的基础上,首要目标是改进民主(环境)决策程序,以便使大多数人能够参与规划和决策,并能够了解这些决策的重大意义.②LA2l程序寻求以既考虑地方的情况又考虑全球情况的方式改善(至少间接地)当地广义的环境状况.本文的第一部分探讨了LA21的概念与方法并阐明对于波罗的海LA2l非常重要的不同的行动领域.此外,研究论文还将描述和展示在一个城市网,即波罗的海城市联盟(uBC)范围内的LA2l形势.包括传递信息、模型及观念的联网是将LA2l理念传播到特别是新近民主化的社会的一个主要手段.最后,本文将对波罗的海边缘的LA2l形势作一全面的评价.     

Recombinant luminescent bacterial sensors for the measurement of bioavailability of cadmium and lead in soils polluted by metal smelters

Environmental hazard of heavy metals in soils depends to a large extent on their bioavailability. The approach used in this study enables the determination of bioavailable metals in solid-phase samples. Two recombinant bacterial sensors, one responding specifically to cadmium and the other to lead and cadmium by increase of luminescence (firefly luciferase was used as a reporter) were used to determine the bioavailability of these metals in soil-water suspensions (a contact assay) and respective particle-free extracts. Fifty agricultural soils sampled near zinc and lead smelters in the Northern France containing up to (mg/kg) 20.1 of Cd, 1050 of Pb and 1390 of Zn were analysed. As the soil matrix interferes with the assay, recombinant luminescent control bacteria lacking the metal recognizing protein and corresponding promoter (thus, being not metal-inducible) but otherwise comparable to the sensor bacteria (the same host bacterium and plasmid encoding luciferase) were used in parallel to take into account the possible quenching and/or stimulating effects of the sample on the luminescence of the sensor bacteria. Both, chemical and sensor analysis showed that only microg/l levels of metals were extracted from the soil into the water phase (0.1% of the total Cd, 0.07% of Pb and 0.5% of Zn). However, 115-fold more Cd and 40-fold more Pb proved bioavailable if the sensor bacteria were incubated in soil suspensions (i.e., in the contact assay). The bioavailability of metals in different soils varied (depending probably on soil type) ranging from 0.5% to 56% for cadmium and from 0.2% to 8.6% for lead.     

Bioluminescent yeast assays for detecting estrogenic and androgenic activity in different matrices

In this paper we describe the construction and use of a set of bioluminescent yeast strains for the detection of compounds that can affect androgen or estrogen receptor mediated hormonal signalling. The set includes Saccharomyces cerevisiae strains expressing human androgen receptor (AR), estrogen receptor alpha (ERalpha) or estrogen receptor beta (ERbeta), along with firefly luciferase controlled by a respective hormone responsive promoter. A constitutively luminescent strain was included in the set for determining the cytotoxicity of the sample. Yeast cells were incubated with pure chemicals or complex samples for 2.5 h, after which the signal could be detected from the cell-sample mixture after simply adding the D-luciferin substrate. The assays could be completed in one day and they required no cell lysis or centrifugation steps, which makes them suitable for high-throughput analysis of samples. Due to a short incubation time the assays are directly applicable to different sample matrices, requiring no pretreatment of the samples. The assays were used to assess the hormonal activity in moisturizing lotions as an example of a complex sample matrix known to contain endocrine disrupting chemicals. Six out of eight tested moisturisers showed high estrogenic activity, whereas no androgenic activity was observed in the samples.     

Impact of aquacultures on the marine ecosystem: Modelling benthic carbon loading over variable depth

In order to be used within Environmental Impact Assessment study, we have developed a three-dimensional particle tracking model for prediction of benthic carbon loading (BCL) caused by fish farms. The model is based on stochastic differential equations for particle transport consistent with the well-known semi-empirical advection/diffusion equation. It requires only easily obtainable input data in the form of measured current record, the source location and a specification of local bathymetry. The model accounts for advection by long-term residual and tidal currents, turbulent diffusion, realistic bathymetry and variations in daily (monthly or yearly) emissions from fish farm.Here, we concentrate on the changes in sedimentation pattern caused by various bathymetric shapes. Examination of idealized cases reveals where and why we can expect the worst impact on benthic communities. For future reference, these results will be included into guidelines for fish farming.     

Transport and Diffusion of Ozone in the Nocturnal and Morning Planetary Boundary Layer of the Phoenix Valley

The evolution of ozone (O ₃) in the nocturnal and morning-transitional planetary boundary layer (PBL) of the Phoenix valley was measured as a part of the `Phoenix Sunrise Experiment 2001' of the U.S. Department of Energy conducted in June 2001. The goal of the field program was to study the transport, distribution and storage of ozone and its precursors in the urban boundary layer over a diurnal cycle. The ground level O <sub>3</sub> as well as mean meteorological variables and turbulence were measured over the entire period, and vertical profiling (using a tethered balloon) was made during the morning transition period. Approximately half of the observational days showed the usual diurnal cycle of high O <sub>3</sub> during the day and low O <sub>3</sub> at night, with nitrogen oxides (NO <sub>x</sub> = NO <sub>2</sub> + NO) showing an out of phase relationship with O <sub>3</sub>. The rest of the days were signified by an anomalous increase of O <sub>3</sub> in the late evening ( 2200 LST), concomitant with a sudden drop of temperature, an enhancement of wind speed and Reynolds stresses, a positive heat flux and a change of wind direction. NO <sub>x</sub> measurements indicated the simultaneous arrival of an `aged' air mass, which was corroborated by the wind predictions of a mesoscale numerical model. In all, the results indicate that the recirculation of O ₃ rich air masses is responsible for the said high-O ₃ events. Such air masses are produced during the transport of O ₃ precursors by the upslope flow toward mountainous suburbs during the day, and they return back to the city at night via downslope winds (i.e. mountain breeze). The corresponding flow patterns, and hence the high-O ₃ events, are determined by background meteorological conditions. The vertical profiling of O ₃ and flow variables during the morning transition points to a myriad of transport, mixing and chemical processes that determine the fate of tropospheric O ₃. How well such processes are incorporated and resolved in predictive O ₃ models should determine the accuracy of their predictions.     

Exposure of garbage truck drivers and maintenance personnel at a waste handling centre to polycyclic aromatic hydrocarbons derived from diesel exhaust

Exposure to diesel exhaust was evaluated in summer and winter by measuring vapour and particle phase polycyclic aromatic hydrocarbons (PAHs). Fifteen PAHs were simultaneously determined from the air samples obtained from truck drivers collecting household waste and maintenance personnel at a waste handling centre. The major compounds analysed from the personal air samples of exposed workers were naphthalene, phenanthrene and fluorene. The total PAH exposure (sum of 15 PAHs) of garbage truck drivers ranged from 71 to 2,660 ng m(-3) and from 68 to 900 ng m-3 in the maintenance work. The exposure of garbage truck drivers to benzo[a]pyrene (B[a]P) ranged from the mean of 0.03 to 0.3 ng m(-3) whereas no B[a]P in control samples or in those collected from maintenance workers was detected. A statistically significant difference in diesel-derived PAH exposure between the garbage truck drivers and the control group in both seasons (in summer p = 0.0022, degrees of freedom (df) 70.5; and in winter p < 0.0001, df = 80.4) was observed. Also, a significant difference in PAH exposure between the garbage truck drivers and the maintenance workers (in summer p < 0.0001, df = 50.1; and in winter p < 0.0001, df = 44.2) was obtained.