Comparative analysis on the genetic population structures of the deep-sea whelks <Emphasis Type="Italic">Buccinum tsubai</Emphasis> and <Emphasis Type="Italic">Neptunea constricta</Emphasis> in the Sea of Japan

The population structures of Buccinum tsubai and Neptunea constricta were compared using partial sequences of the mitochondrial COI gene. B. tsubai included four genetically distinguishable subpopulations (Hokkaido, Yamagata-Toyama, Yamato Bank, and San’in), which is consistent with the results of our previous study using mitochondrial 16SrRNA sequences. The genetic structure of N. constricta was also clear, but the lineage sorting corresponding to the geographical distribution was not as clear as that of B. tsubai. The difference between the genetic structures of these two species might be due to a difference in either their dispersal abilities or the historic distribution patterns in the Sea of Japan. The fossil record and nested clade analyses support the latter alternative. The four subpopulations of B. tsubai are estimated to have diverged 0.42–1.46 million years ago, providing support for the existence of intermediate water of normal salinity and oxygen concentration capable of sustaining B. tsubai between the low-salinity surface water and anoxic deep water during the Pleistocene glacial periods. The genetic diversity of the Yamato Bank subpopulation of B. tsubai was lower than that of the other subpopulations, which is likely attributable to the small size of the Yamato Bank subpopulation.     

Measuring marginal willingness to pay using conjoint analysis and developing benefit transfer functions in various Asian cities

We need a consistent methodology to measure the co-benefits of climate change mitigation across Asian countries. This study chose a strategy of modifying the Japan-specific life-cycle impact assessment method based on endpoint modeling (LIME) for wider application across countries. LIME has two dimensions. First, it is an environmental science that links the cause-and-effect chain. Second, it is an environmental valuation that weighs four endpoint damages in monetary terms through a conjoint analysis that is derived from an Internet-based questionnaire survey. This article describes the modification of the methodology for application of the conjoint analysis to weigh environmental impacts. We approached the investigation as follows. First, we conducted Internet surveys to measure marginal willingness-to-pay (MWTP). We used a sample of 112 respondents in their 20 s to 40 s, divided equally between men and women, in 11 cities across China, India, and Southeast Asia. The results obtained showed clear statistical significance and were comparable across the cities. Second, we attempted to develop functions (called benefit transfer functions) to simplify the measured MWTP in order to apply it across different Asian countries. The functions were derived through a stepwise meta-analytic method, a type of multiple regression analysis whose independent variable was MWTP and dependent variables were attributes of both respondents and surveyed cities. The functions showed that coal consumption and percentage of nature reserve were dependent variables. Then, the MWTPs estimated from the functions were compared with the measured MWTP for transfer error, which is calculated by the absolute value of the difference between the estimated value and the measured value divided by the latter. The transfer error was below 50% in about 90% of the 44 results (a combination of four endpoints and 11 cities), implying that the developed functions were statistically significant.     

Enhanced decomposition of (4-chloro-2-methylphenoxy) acetic acid by combined ultrasonic and oxidative treatment

Combined ultrasonic (US) irradiation and sodium peroxodisulfate (Na₂S₂O₈) treatment has been investigated for promotion of both decomposition of (4-chloro-2-methylphenoxy) acetic acid (MCPA) and mineralization of organic residues. This treatment is expected to accelerate both reactions, because the US cavitation effect promotes the production of radicals, such as SO ₄ ^? · and OH·, by the decomposition of Na₂S₂O₈ and water. In this study, decomposition experiments were performed on 100?ppm MCPA aqueous solutions in a sonoreactor at reaction temperatures of 298?C333?K with US irradiation alone, Na₂S₂O₈ treatment alone, and the combination of US and Na₂S₂O₈ treatment. It was found that the combined treatment achieved a higher MCPA decomposition rate and total organic carbon (TOC) removal ratio than either treatment alone. The decomposition ratios of both MCPA and TOC increased with reaction temperature, and especially steep increases were observed at 333?K due to a significant promotion of thermal decomposition of Na₂S₂O₈. The production of radical species was also promoted by the combined treatment. These results suggest that the higher MCPA decomposition rate and TOC removal ratio are due to the increased formation of sulfate and hydroxyl radicals via thermal and US decomposition of Na₂S₂O₈.     

Study of the Determination of the Ultimate Aerobic Biodegradability of Plastic Materials Under Controlled Composting Conditions

Several ISO standards for determining the ultimate aerobic/anaerobic biodegradability of plastic materials have been published. In particular, ISO 14855-1 is a common test method that measures evolved carbon dioxide using such methods as continuous infrared analysis, gas chromatography or titration and others (ISO 14855-1(2005.9)). This method is a small-scale test for determining the ultimate aerobic biodegradability of plastic materials, where the amounts of compost inoculum and test sample in one tenth comparing with that of ISO 14855-1. This method is well versed in ISO/DIS 14855-2 which the carbon dioxide evolved from test vessel is determined by gravimetric analysis of carbon dioxide absorbent. The focus of this study is to elucidate statistically the results of round robin test by seven countries used MODA, which were various deviations among the experiments.     

Effects of Nitrogen Supply on the Sensitivity to O3 of Growth and Photosynthesis of Japanese Beech (Fagus crenata) Seedlings

To obtain basic information for evaluating critical levels of O₃ under different nitrogen loads for protecting Japanese beech forests, two-year-old seedlings of Fagus crenata Blume were grown in potted andisol supplied with N as NH₄NO₃ solution at 0, 20 or 50 kg ha⁻¹ year⁻¹ and exposed to charcoal-filtered air or O₃ at 1.0, 1.5 and 2.0 times the ambient concentration from 16 April to 22 September 2004. The O₃ induced significant reductions in the whole-plant dry mass, net photosynthetic rate at 380 μmol mol⁻¹ CO₂ (A ₃₈₀), carboxylation efficiency (CE) and concentrations of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and total soluble protein (TSP) in the leaves. The concentrations of Rubisco and TSP were negatively correlated with the concentration of leaf acidic amino acid, suggesting that O₃ enhanced the degradation of protein such as Rubisco. The N supply to the soil did not significantly change the whole-plant dry mass and A ₃₈₀, whereas it significantly increased the CE and concentrations of Rubisco and total amino acid. No significant interactive effects of O₃ and N supply to the soil were detected on the growth, photosynthetic parameters and concentrations of protein and amino acid in the leaves. In conclusion, N supply to the soil at ≤50 kg ha⁻¹ year⁻¹ does not significantly change the sensitivity to O₃ of growth and net photosynthesis of Fagus crenata seedlings.     

Hydroxyl radical concentration profile in photo-Fenton oxidation process: generation and consumption of hydroxyl radicals during the discoloration of azo-dye Orange II

Dynamic behaviors of hydroxyl (OH) radical generation and consumption in photo-Fenton oxidation process were investigated by measuring OH radical concentration during the discoloration of azo-dye Orange II. The effects of operating parameters for photo-Fenton discoloration, i.e. dosages of H₂O₂ and Fe, initial dye concentration, solution pH and UV irradiation, on the generation and consumption of OH radicals playing the main role in advanced oxidation processes were extensively studied. The scavenger probe or trapping technique in which coumarin is scavenger of OH radical was applied to estimate OH radical concentration in the photoreactor during the photo-Fenton discoloration process. The OH radical generation was enhanced with increasing the dosages of Fenton regents, H₂O₂ and Fe. At the initial stage of photo-Fenton discoloration of Orange II, the OH radical concentration rapidly increased (Phase-I) and the OH radical concentration decreased after reaching of OH radical concentration at maximum value (Phase-II). The decrease in OH radical concentration started when the complete discoloration of Orange II was nearly achieved and the H₂O₂ concentration became rather low. The dynamic behavior of OH radical concentration during the discoloration of Orange II was found to be strongly linked with the change in H₂O₂ concentration. The generation of OH radical was maximum at solution pH of 3.0 and decreased with an increase of solution pH. The OH radical generation rate in the Fenton Process was rather slower than that in the photo-Fenton process.     

Measuring future dynamics of genuine saving with changes of population and technology: application of an integrated assessment model

Theoretical and empirical studies have been conducted on the genuine saving (GS) based on neoclassical economic theory to assess sustainable development (SD). However, only market prices and statistical national accounts have been used in empirical studies due to limited data availability. The data availability limits to measure GS only in the past and current, causing a wide gap with theoretical results. In this paper, we propose computing GS using an integrated assessment model (IAM) as connected to the mainframe model of macroeconomy. This enables us to use shadow prices, rather than market prices, obtained through an IAM, which ensures substantial consistency among variables. An example would be endogenous capital–output ratio and the rate of TFP. Also, our indicator of GS is more comprehensive in that they now account for various resources, environmental degradation, and land use. Our simulation results, with a particular focus on GS with population change (GSn) and with technological change as well (GSnt), show a sustainable future for up to the end of the century thanks to declining population in the latter half of the century and technological progress, although GS without accounting for population and technology tend to be negative, driven by, among others, capital depreciation and net primary productivity degraded by land use.     

Ecological effects of imidacloprid on arthropod communities in and around a vegetable crop

To elucidate the ecological impacts of imidacloprid [1-(6-chloro-3-pyridylmethyl)-N-nitroimidazolidin-2-ylideneamine] on vegetable crops and their surrounding areas, arthropods on eggplant patches treated with this insecticide were monitored throughout two cultivation seasons, spring and autumn, and the results compared with those of control crops. Residues of imidacloprid in soil accounted for 4-8% of total applied, and concentrations outside the crop were always below 5 micro g/kg. Communities of the crop vegetation experienced significant density reductions, mostly of aphids, and had fewer species diversity during the first month. Those in the surrounding pasture were similarly affected though with less intensity, while non-target ground arthropods both inside and outside the crop only showed significant impacts in the two weeks after planting.     

Trace metal contamination in commercial fish and crustaceans collected from coastal area of Bangladesh and health risk assessment

Environmental Science and Pollution Research - Trace metals contamination in commercial fish and crustaceans have become a great problem in Bangladesh. This study was conducted to determine seven...