东亚三角涡虫监测水体重金属污染的可行性研究

随着现代工、农业的快速发展,水体重金属污染已成为全球性的环境问题。对水体重金属污染的现状、来源、危害以及监测方法进行了介绍,并探讨了生物监测技术的优越性。在前人的研究基础上,结合本实验室工作,提出利用东亚三角涡虫(Dugesiaojapoice)作为水体重金属污染监测指示生物的可行性,并对更为灵敏的生物标志物的策略和实验方案进行了展望。     

北京市门头沟区生态安全评估及预测

为研究和预测北京市门头沟地区生态安全形势,并在实践中为生态安全评价提供理论依据,在生态环境管理的方法论基础上,根据统计年鉴,采用压力状态响应模型建立生态安全指标体系,以不安全指数法对指标实际值归一化,运用层次分析法确定指标权重,采用加权指数法计算生态安全综合值,评价了门头沟区3年的生态安全状况。结果表明:门头沟地区2006-2008年3年期间,生态安全综合指数分别为:0.6192、0.639 3、0.6395。显示生态安全综合值缓慢上升,生态安全越来越好,但是仍然处于较不安全状态。指出其主要原因有经济欠发达、自然资源缺乏、人口众多等。建议该地区加快产业结构调整、改变增长方式、控制人口、提高土地利用率,保护环境并治理污染。     

Detection Method for Avian Influenza Viruses in Water

Recent events have shown that humans may become infected with some pathogenic avian influenza A viruses (AIV). Since soil and water, including lakes, rivers, and seashores, may be contaminated by AIV excreted by birds, effective methods are needed for monitoring water for emerging viruses. Combining water filtration with molecular methods such as PCR is a fast and effective way for detecting viruses. The objective of this study was to apply a convenient method for the detection of AIV in natural water samples. Distilled water and lake, river, and seawater were artificially contaminated with AIV (H5N3) and passed through a filter system. AIV was detected from filter membrane by real-time RT-PCR. The performance of Zetapor, SMWP, and Sartobind D5F membranes in recovering influenza viruses was first evaluated using contaminated distilled water. SWMP, which gave the highest virus recoveries, was then compared with a pre-filter combined GF/F filter membrane in a trial using natural water samples. In this study, the cellulose membrane SMWP was found to be practical for recovery of AIVs in water. Viral yields varied between 62.1 and 65.9% in distilled water and between 1 and 16.7% in natural water samples. The borosilicate glass membrane GF/F combined with pre-filter was also feasible in filtering natural water samples with viral yields from 1.98 to 7.33%. The methods described can be used for monitoring fresh and seawater samples for the presence of AIV and to determine the source of AIV transmission in an outbreak situation.     

Prevalence of Norovirus Infection in Children and Adults with Acute Gastroenteritis,Tehran, Iran, 2008–2009

Noroviruses are one of important agents that cause acute viral gastroenteritis worldwide. These viruses are belonging to Caliciviridae family and are genetically diverse. To date, there is no valuable data about prevalence of norovirus infection and the dominant genogroup/genotype among Iranian population. The objective of this study was to determine the frequency of norovirus infection in Iranian patients with gastroenteritis referred to three hospitals of Tehran and to specify the dominant genogroup/genotype of this virus among our study population. A total of 293 patients with acute gastroenteritis were included in the study. Detection of norovirus was performed using RT-PCR method and confirmed by direct sequencing with specific designed primers for capsid region of norovirus genome. Phylogenetic analysis was performed using the neighbor-joining method. Norovirus strains identified in our study were subsequently categorized according to previously defined genogroup/genotypes. Of these, norovirus GII was dominant genogroup. Sixty-five percent (17 of 26) of positive samples were determined as GII and 35% (9 of 26) were determined as GI, respectively, in 2008–2009. And among 8 sequenced strains of genogroup II the most frequent genotype was GII.3. The results of this study indicated that norovirus must be considered as one of the infectious causes of acute gastroenteritis among Iranian population. We also found that GII.3 is more prevalent in our study population. To the best of our knowledge there is limited data about the role of noroviruses in children and adults’ acute gastroenteritis among Iranian patients and this prevalence and genotyping report of norovirus infection could be remarkable for further studies.     

安徽省TJ-Ⅱ型钻孔体应变同震效应分析

描述了安徽省地震局"十一五"期间安装的5套钻孔体应变仪的地质构造特点与钻孔岩芯物理特性,介绍了TJ-Ⅱ体应变仪的运行情况。通过对两次强震活动的同震效应分析,初步探讨了地理、地质构造条件不同的情况下,各观测井对记录应力活动能力强弱的反映。     

中国火山地区预警政策研究

本文主要介绍了我国的活火山、火山灾害情况及国内外的火山预警政策。在此基础上讨论了我国火山地区预警政策的组成结构,认为完整的火山地区预警政策应该包括活火山识别、火山风险评价与灾害区划图、制定火山应急预案、火山防灾减灾知识宣传、火山监测和灾害预报、火山灾害预警等级划分、火山减灾工程措施以及预警响应措施等方面,并对火山预警政...     

Characterization of chlorine dioxide as disinfectant for the removal of low concentration microcystins

Microcystins, which represents one kind of cancerogenic organic compounds, is abundant in eutrophication water. The effects of reaction factors on chlorine dioxide (ClO₂) for removal of low-concentration Microcystin-LR, Microcystin-RR, and Microcystin-YR in water as well as the reaction mechanisms was investigated by using enzyme-linked immunosorbent assay (ELISA) kit and gas chromatography-mass spectrometry (GC-MS). The results showed that MC-LR, MC-RR, and MC-YR could be efficiently decomposed by ClO₂. The degradation efficiency was shown positively correlated to the concentration of ClO₂ and reaction time; while the effect of reaction temperature and pH is slight. The kinetic constants and activation energies of the reaction of MC-LR, MC-RR, and MC-YR with ClO₂ are determined as 459.89, 583.15, 488.43 L·(mol·min)⁻¹ and 64.78, 53.01, 59.15 kJ·mol⁻¹, respectively. As indicated by high performance liquid chromatography mass spectrometer (HPLC-MS) analysis, degradation should be accomplished via destruction of Adda group by oxidation, with the formation of dihydroxy substituendums as end products. This study has provided a fundamental demonstration of ClO₂ serving as oxidizing disinfectant to eliminate microcystins from raw water source.     

含砷毒剂降解产物三苯胂对大豆萌发及幼苗生长的影响

采用不同浓度三苯胂(Triphenylarsine,TPA)沙质室内培养东北代表性作物大豆,研究日本遗弃在华化学武器("日遗化武")装填毒剂的主要降解产物之——TPA(C18H15As)对大豆萌发和幼苗生长的影响。结果表明:在0 mg/kg～400 mg/kg TPA浓度范围内,大豆受到的毒性效应随浓度增大而增大,直至无法生长;试验大豆的萌发率、根长、下胚轴长和株高等形态指标均随着培养介质中TPA浓度的增加而出现抑制效应明显增加的趋势;其中根系是大豆幼苗期砷累积的主要器官,也是对环境TPA毒害效应最敏感、响应最早的部位之一。日遗华武泄漏造成的土壤污染若处理不当会对埋藏地种植的大豆作物生长产生影响。