An individual and a sex odor signature in kittiwakes? Study of the semiochemical composition of preen secretion and preen down feathers

The importance of olfaction in birds’ social behavior has long been denied. Avian chemical signaling has thus been relatively unexplored. The black-legged kittiwake provides a particularly appropriate model for investigating this topic. Kittiwakes preferentially mate with genetically dissimilar individuals, but the cues used to assess genetic characteristics remain unknown. As in other vertebrates, their body odors may carry individual and sexual signatures thus potentially reliably signaling individual genetic makeup. Here, we test whether body odors in preen gland secretion and preen down feathers in kittiwakes may provide a sex and an individual signature. Using gas chromatography and mass spectrometry, we found that male and female odors differ quantitatively, suggesting that scent may be one of the multiple cues used by birds to discriminate between sexes. We further detected an individual signature in the volatile and nonvolatile fractions of preen secretion and preen down feathers. These results suggest that kittiwake body odor may function as a signal associated with mate recognition. It further suggests that preen odor might broadcast the genetic makeup of individuals, and could be used in mate choice to assess the genetic compatibility of potential mates.     

Pastoral farmland habitat diversity in south-east Ireland

In this study, habitat surveys were undertaken on 50 grass-based farms in SE Ireland and data digitised onto aerial photography. Additional data i.e. stocking rates, and participation (or otherwise) in the Irish Rural Environment Protection Scheme (REPS) were collected and analysed as possible explanatory variables for farm habitat composition.Results indicated that approximately 14.3% of the land area of sampled farms comprised of semi-natural habitat types, a proportion substantially greater than has been reported for many other European countries. The most frequently recorded semi-natural habitats included, field boundaries, scrub, and deciduous and riparian woodlands.Multivariate analysis of farm habitat configuration showed a strong dichotomy between dairy and non-dairy farming systems. Habitats such as intensively managed grassland and built ground were closely associated with dairy-based enterprises. In contrast, the incidence of other habitat types was associated with non-dairy and/or REPS participating enterprises. The ecological quality of field boundaries as assessed by the Field Boundary Evaluation and Grading System (FBEGS) Index was significantly greater on dairy, compared with dry-stock farms.This dichotomy in farm habitat composition is not reflected within current Agri-Environment (AE) policy. Integration of locally important drivers of habitat diversity into the design and implementation of AE policy, is integral to the successful performance of AE schemes.     

Application of winery and distillery waste composts to a Jumilla (SE Spain) vineyard: Effects on the characteristics of a calcareous sandy-loam soil

The effects on different physico-chemical, chemical and biological soil properties of the incorporation of different organic materials into a calcareous vineyard soil were studied in an experiment during three growing seasons. The organic materials used were sheep manure and four composts derived from the treatment of winery and distillery wastes. A multivariate technique, factor analysis (FA), was also used for interpreting the data obtained from the samplings carried out throughout the three vine growing seasons. The application of the organic materials induced an increase in the activity of the soil microorganisms and in the soil macro and micronutrient contents, as well as a slow release of inorganic N. Through FA of the soil properties studied, three factors that explained 74.7% of the variability were established and the effects of the treatments after the whole experimental period were assessed.     

Anatomical localization and stereoisomeric composition of <Emphasis Type="Italic">Tribolium castaneum</Emphasis> aggregation pheromones

We report that the abdominal epidermis and associated tissues are the predominant sources of male-produced pheromones in the red flour beetle, Tribolium castaneum and, for the first time, describe the stereoisomeric composition of the natural blend of isomers of the aggregation pheromone 4,8-dimethyldecanal (DMD) in this important pest species. Quantitative analyses via gas chromatography–mass spectrometry showed that the average amount of DMD released daily by single feeding males of T. castaneum was 878 ± 72 ng (SE). Analysis of different body parts identified the abdominal epidermis as the major source of aggregation pheromone; the thorax was a minor source, while no DMD was detectable in the head. No internal organs or obvious male-specific glands were associated with pheromone deposition. Complete separation of all four stereoisomers of DMD was achieved following oxidation to the corresponding acid, derivatization with (1R, 2R)- and (1S, 2S)-2-(anthracene-2,3-dicarboximido)cyclohexanol to diastereomeric esters, and their separation on reversed-phase high-performance liquid chromatography at −54°C. Analysis of the hexane eluate from Porapak-Q-collected volatiles from feeding males revealed the presence of all four isomers (4R,8R)/(4R,8S)/(4S,8R)/(4S,8S) at a ratio of approximately 4:4:1:1. A walking orientation bioassay in a wind tunnel with various blends of the four synthetic isomers further indicated that the attractive potency of the reconstituted natural blend of 4:4:1:1 was equivalent to that of the natural pheromone and greater than that of the 1:1 blend of (4R,8R)/(4R,8S) used in commercial lures.     

Soil fertility dynamics in runoff-capture agriculture, Canary Islands, Spain

In the extremely arid (∼150 mm yr⁻¹) eastern Canary Islands of Lanzarote, Fuerteventura and La Graciosa, agriculture has been sustained for decades by a traditional runoff-capture (RC) farming system known as “gavias”. Although the main goal of these systems is to increase water supply for crops, making unnecessary conventional irrigation, a secondary and equally important factor is that this system allows for sustainable agricultural production without addition of chemical or organic fertilizers. A field study was conducted to assess the impact of long-term agriculture (>50 yr) on soil fertility and to evaluate key factors affecting the nutrient sustainability of RC agricultural production. Soil fertility and nutrient dynamics were studied through chemical characterization of the arable layer (0-25 cm) of RC agricultural plots, adjacent natural soils (control) not affected by runoff and cultivation, and sediments contributed by a series of RC events. Results showed that RC soils have enhanced fertility status, particularly because they are less affected by salinity and sodicity (mean electrical conductivity = 1.8 dS m⁻¹ vs. 51.0 dS m⁻¹ in control soils; mean exchangeable sodium percentage = 11.1% vs. 30.6% in control soils), and have higher water and nutrient holding capacities (mean clay plus silt contents ≈87% vs. 69% in control soils). In general, sediments transported with the runoff and deposited in RC plots (average sediment yield ≈ 46 ton ha⁻¹ yr⁻¹), contain sufficient nutrients to prevent a progressive reduction of essential plant nutrients below natural levels in spite of nutrient uptake and removal by the harvested crop. Average additions of nitrogen, phosphorus and potassium with the runoff sediments were 33.6, 35.3 and 48.8 kg ha⁻¹ yr⁻¹, respectively. Results of this study show how a crop production system can be sustained in the long term by natural hydrological and biogeochemical catchment processes. This system maintains a nutrient balance that is not based on energy-intensive inputs of fertilizers, but is integrated in natural nutrient cycling processes, unlike other tropical farming agroecosystems.     

Spatial and temporal variations in groundwater nitrate at an intensive dairy farm in south-east Ireland: Insights from stable isotope data

Achievement of at least “good ecological status” in all waterbodies under the EU Water Framework Directive by 2015 will in some cases be a challenge. The twin challenge is to manage expectations of policy makers for such waterbodies as to a realistic length of time required for improvement in water quality. Hence, understanding the source, transformation processes and residence time of nitrate in a hydrological system is an essential part of meeting such challenges. On a dairy farm with 24 shallow groundwater wells, the dual isotopic composition of nitrate (δ¹⁵N and δ¹⁸O) was used to clarify nitrate sources, to assess spatial and temporal variability in nitrate concentrations and to determine if and where denitrification was occurring. Vertical travel time was estimated to correlate nitrate concentrations with management practices. Organically derived nitrogen was the predominant source contributing to groundwater nitrate concentrations. Denitrification was identified as prevalent within specific regions of the study site. The distinct low temporal variability in the isotopic data suggests constancy among nitrate sources and processes over time across the study site. Vertical travel times of up to 3 years were estimated on site indicating the influence of recent management practices on nitrate concentrations. Very slow horizontal migration of groundwater (decades) indicates a legacy of older management practices. Stable isotope techniques, together with an understanding of time lag, provide an extra mechanism to test the efficacy of monitoring and mitigation programmes.     

Fertilization trajectory of the root crop Raphanus sativus across atmospheric pCO2 estimates of the next 300 years

Plant biomass is known to increase in response to elevated atmospheric CO₂ concentration (pCO₂); however, no experiments have quantified the trajectory of crop fertilization across the full range of pCO₂ levels estimated for the next 300 years. Here we quantify the above- and below-ground biomass response of Raphanus sativus (common radish) across eight pCO₂ levels ranging from 348 to 1791 ppmv. We observed a large net biomass increase of 58% above ground and 279% below ground. A large part of the net increase (38% of the above-ground and 53% of the below-ground) represented biomass fertilization at the high levels of pCO₂ (700–1791 ppmv) predicted if fossil fuel emissions continue unabated. The trajectory of below-ground fertilization in R. sativus greatly exceeded a trajectory based on extrapolation of previous experiments for plants grown at pCO₂ < 800 ppmv. Based on the experimental parameters used to grow these plants, we hypothesize that these experiments represent the maximum CO₂ fertilization that can be achieved for this plant growing under low light levels. If the below-ground biomass enhancement that we have quantified for R. sativus represents a generalized root-crop response that can be extrapolated to agricultural systems, below-ground fertilization under very high pCO₂ levels could dramatically augment crop production in some of the poorest nations of the world, provided that water resources are sufficient and sustainable.     

Minimizing leakage from a smelter off-gas system by enhanced control

The off-gas handling system is a vital component of a smelter and it is essential that it operates in accordance with environmental regulations. A key feature of the off-gas management system is maintaining a slightly negative system pressure to prevent gas leakage, such as SO₂, out into the work and external environments. To enhance sustainable development, there is also growing interest in capturing and reusing the significant amount of heat and CO₂ contained within off-gas. The potential incorporation of heat and CO₂ recovery systems places importance on enhancing control to minimize the risk of both gas leakage and disturbance of the smelting process. With these requirements in mind, an easily implementable feedforward control enhancement to existing control schemes is proposed to help provide improved performance.     

Applying the precautionary principle to consumer household cleaning product development

Hazardous chemicals are pervasive in household disinfectant products. Many ingredients have established associations with acute and chronic human health conditions as well as with environmental damage. Although these associations are suggested but not proven, they are of great concern. This article describes the application of the precautionary principle to the selection of an anti-microbial active ingredient for a botanical disinfectant when significant uncertainty exists around the hazard and risk of traditional disinfectant active ingredients. We show that application of the precautionary principle does not stifle innovation and facilitates a responsible approach to product development.