Inhibition of erythrocyte delta-aminolevulinic acid dehydratase (ALAD) activity in fish from waters affected by lead smelters

We assessed the effects on fish of lead (Pb) released to streamsby smelters located in Trail, BC (Canada), E. Helena, MT, Herculaneum, MO, and Glover, MO. Fish were collected by electrofishing from sites located downstream of smelters and from reference sites. Blood from each fish was analyzed for -aminolevulinic acid dehydratase (ALAD) activity and hemoglobin (Hb), and samples of blood, liver, or carcass were analyzed for Pb, zinc (Zn), or both. Fish collected downstreamof all four smelters sites had elevated Pb concentrations, decreased ALAD activity, or both relative to their respectivereference sites. At E. Helena, fish from the downstream site also had lower Hb concentrations than fish from upstream. Differences among taxa were also apparent. Consistent with previous studies, ALAD activity in catostomids (Pisces: Catostomidae-northern hog sucker, Hypentelium nigricans;river carpsucker, Carpiodes carpio; largescale sucker, Catostomus macrocheilus; and mountain sucker, C. platyrhynchus) seemed more sensitive to Pb-induced ALAD inhibition than the salmonids (Pisces: Salmonidae-rainbow trout,Oncorhynchus mykiss; brook trout, Salvelinus fontinalis) or common carp (Cyprinus carpio). Some of these differences may have resulted from differential accumulation of Zn, which was not measured at all sites. We detected no ALAD activity in channel catfish (Ictaluruspunctatus) from either site on the Mississippi River at Herculaneum, MO. Our findings confirmed that Pb is releasedto aquatic ecosystems by smelters and accumulated by fish, andwe documented potentially adverse effects of Pb in fish. We recommend that Zn be measured along with Pb when ALAD activityis used as a biomarker and the collection of at least 10 fish ofa species at each site to facilitate statistical analysis.     

Hydroxyl radical generation by electron paramagnetic resonance as a new method to monitor ambient particulate matter composition

Epidemiological studies have demonstrated the relationship between exposure to ambient particulate matter (PM) and health effects in those with cardiopulmonary diseases. The free radical generating activity of particles has been suggested as a unifying factor in the biological activity of PM in toxicological studies but so far has not been applied as a method for environmental monitoring of PM. The purpose of this study was to characterize hydroxyl radical (OH*) production by different size fractions of PM, to use as an alternative method for monitoring of PM composition and activity. We have developed a method, using electron paramagnetic resonance (EPR), to measure OH* radical formation in suspensions of particles in the presence of hydrogen peroxide and 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) as a specific spin-trap. Samples of ambient particulate matter (PM) of different size fractions were collected from various sites on various filters. PM deposited on filters as well as suspensions in water retain its ability to generate OH* and this generation is determined by concentration of hydrogen peroxide and soluble metals. However, large variations in OH* radical formation and kinetics were found with different soluble metals and within metals (Fe, V) with different valencies. The method was applied to environmental monitoring in Hettstedt-Zerbst, situated in South-Eastern Germany, where it showed a relation to Cu-content of PM. The method was also applied in Duisburg, where the PMI fraction showed the highest DMPO-OH* generation but was not linked to particle counts. The method integrates metal bioavailability and reactivity and can provide a better understanding of the effect of small variations in mass concentrations on health.     

Weighing the evidence: prospects for evidence-based policy-making

Evidence-based policy (EBP) is currently one of the "big ideas" in policy circles. From its origins in the medical community, EBP is now being applied to other policy domains including environment. But will it really ensure that the public policy grease gets to the environmental squeak? And what are the implications for scientists themselves?     

How well should laboratories perform in proficiency tests

The long-term performance of laboratories is examined using results from three proficiency testing programmes in the water and environmental monitoring sector. A medium term proficiency test pass rate of greater than approximately 75% is suggested as a minimum target for routine analysis to demonstrate fitness for purpose. This pass rate is proposed as a practical yardstick to be used by QA managers and accreditation bodies. This recommendation is contingent on a laboratory's continued and largely uninterrupted participation in a PT programme.     

EFFECTS OF CLIMATE VARIABILITY ON RIVERS: CONSEQUENCES FOR LONG TERM WATER QUALITY ANALYSIS1

ABSTRACT: Associations between the El Nino Southern Oscillation (ENSO) climate pattern and temporal variability in flow and 12 water quality variables were assessed at 77 river sites throughout New Zealand over a 13‐year period (1989 through 2001). Trends in water quality were determined for the same period. All 13 variables showed statistically significant linear regression relationships with values of the Southern Oscillation Index (SOI). The strongest relationships were for water temperature (mean R²= 0.20), dissolved reactive phosphorus (0.18), and oxidized nitrogen (0.17). The association with SOI varied by climate region. The observed patterns were generally consistent with known ENSO effects on New Zealand rainfall and air temperature. Trends in water quality variables for the periods 1989 through 1993, 1994 through 1998, and 1989 through 1998 were reasonably consistent with trends in SOI, even when the influence of river flow was removed from the data. This suggests that SOI effects on water quality are not necessarily a direct consequence of changes in flow associated with rainfall variation. In addition, both Baseline (32 upstream) and Impact (45 downstream) sites showed similar trends, indicating that changes in management were not directly responsible. We conclude that interpretation of long term water quality datasets in rivers requires that climate variability be fully acknowledged and dealt with explicitly in trend analyses.     

Adapting to Climate Change in Africa

The intersection of present vulnerability and the prospect of climate change in Africa warrants proactive action now to reduce the risk of large-scale, adverse impacts. The process of planning adaptive strategies requires a systematic evaluation of priorities and constraints, and the involvement of stakeholders. An overview of climate change in Africa and case studies of impacts for agriculture and water underlie discussion of a typology of adaptive responses that may be most effective for different stakeholders. The most effective strategies are likely to be to reduce present vulnerability and to enhance a broad spectrum of capacity in responding to environmental, resource and economic perturbations. In some cases, such as design of water systems, an added risk factor should be considered.     

大象将要回到灭绝的老路吗?

尽管有关部门和人员信誓旦旦要采取预防措施，且各种安全保障也被确立，但无论从哪个角度看，2002年11月12日联合濒危动植物种国际贸易公约缔约国大会允许非洲几个国家出售象牙库存的决定必将对国际象牙贸易起到推波助澜的作用，而正是象牙贸易在20世纪80年代使大象走上了灭绝之路。     

Heavy metals in soils and crops in Southeast Asia

In a reconnaissance soil geochemical and plant survey undertaken to study the heavy metal uptake by major food crops in Malaysia, 241 soils were analysed for cation exchange capacity (CEC), organic carbon (C), pH, electrical conductivity (EC) and available phosphorus (P) using appropriate procedures. These soils were also analysed for arsenic (As), cadmium (Cd), cobalt (Co), chromium (Cr), copper (Cu), mercury (Hg), nickel (Ni), lead (Pb) and zinc (Zn) using aqua regia digestion, together with 180 plant samples using nitric acid digestion. Regression analysis between the edible plant part and aqua regia soluble soil As, Cd, Cr, Cu, Hg, Ni, Pb and Zn concentrations sampled throughout Peninsular Malaysia, indicated a positive relationship for Pb in all the plants sampled in the survey (R ² = 0.195, p < 0.001), for Ni in corn (R ² = 0.649, p < 0.005), for Cu in chilli (R ² = 0.344, p < 0.010) and for Zn in chilli (R ² = 0.501, p < 0.001). Principal component analysis of the soil data suggested that concentrations of Co, Ni, Pb and Zn were strongly correlated with concentrations of Al and Fe, which is suggestive of evidence of background variations due to changes in soil mineralogy. Thus the evidence for widespread contamination of soils by these elements through agricultural activities is not strong. Chromium was correlated with soil pH and EC, Na, S, and Ca while Hg was not correlated with any of these components, suggesting diffuse pollution by aerial deposition. However As, Cd, Cu were strongly associated with organic matter and available and aqua regia soluble soil P, which we attribute to inputs in agricultural fertilisers and soil organic amendments (e.g. manures, composts).     

Biology of the basket star <Emphasis Type="Italic">Gorgonocephalus caputmedusae</Emphasis> (L.)

Ophiurid basket stars belonging to the family Gorgonocephalidae are distributed from the Arctic to the Antarctic and from the shallow subtidal to the deep sea, but their biology remains poorly known. In situ observations at the mouth of the Oslofjord by a remotely operated vehicle showed that Gorgonocephalus caputmedusae had a patchy distribution at 85 to 120 m water depth and frequently occurred in association with the gorgonian Paramuricea placomus and the coral Lophelia pertusa. Morphological and histological studies show that G. caputmedusae is well adapted to capture macroplanktonic prey. Histological examination of the arms revealed the presence of a thick layer of dermal mutable connective tissue which is probably an energy-efficient way to maintain its feeding posture against the current. This layer is connected to the nerve cord suggesting that the passive mechanical properties (stiffness) is controlled by the nervous system. In the distal parts of the arms, each segment has a pair of sticky tube feet and a sophisticated system of spines and hooks, which are connected to muscles and collagenous tendons. In combination, these features were shown, in an experimental flume study, to be used for capturing the locally abundant krill species Meganyctiphanes norvegica. This is the first documentation of G. caputmedusae of this kind.     

销售的可持续性

我们所看到的许多资料的研究显示,消费者通常对可持续思想是支持的和要求保证制造商是负责任的.