Restoration of Some Parameters in the Development of Benthos After Reduction of Anthropogenous Loading in the Ecosystem of the Sevastopol Bay in the Black Sea

Research on benthic communities have been carried out in the Sevastopol Bay of the Black Sea for a hundred years. The anthropogenous influence began to have an effect already in the 20–30^th. In this period, all oyster-banks were lost and the mussel-silt and mellina-silt communities have begun to degrade and were not found during almost seventy years in the Bay. The worst state of benthic communities was observed in the 60–90^th, when species of bottom animals were not found over some parts of the sea. In this period, only 10 macrozoobenthic species were registered in the Bay. Mollusks: Nassarius reticulatus, Bittium reticulatum, Cerastoderma glaucum and polychaetes: Nephtys hombergii, Neanthes succinea have proved the most enduring at high anthropogenous loadings The faunistic complex of benthic species registered in 1913 had the greatest similarity to the benthic species complex of 2002. Return of mussel, Melinna palmata polychaete and Ascidiella aspersa sea squirt in benthic communities of the Sevastopol Bay are responsible for the similarity between these faunistic complexes. The restoration of local populations of these benthic species has followed the significant decrease of the anthropogenous loading on the sea ecosystems as a result of the significant reduction of the military naval base in the Bay during the last five to ten years.     

Framing the Long-Term <Emphasis Type="Italic">In Situ</Emphasis> Liability Issue for Geologic Carbon Storage in the United States

This paper examines how legal standards of liability may apply to geologic carbon storage. The liability regime governing geologic carbon storage will shape the technology's cost-effectiveness and overall attractiveness. We classify potential sources of liability into operational, in situ, and climate liability. As a first step, we explore in situ liability in the United States. After summarizing legal standards of liability including negligence, breach of implied warranty, strict liability, and product liability, we discuss how liability may be addressed at the level of the federal government, state government, industry, and the firm. Finally, we address the implications of judicial treatment of liability for carbon storage, including the apportionment of liability and the adequacy of current regulations.     

The Value Of Public Participation During a Hazard,Impact, Risk And Vulnerability (HIRV) Analysis

The first part of this paper discusses the links between hazard, risk and vulnerability (HRV) analysis and the development of mitigative strategies. The second part discusses the need to include public participation when completing an HRV analysis. Two current HRV models are used to illustrate the general failure of HRV analysis to include public participation. The third part of this paper provides a brief overview of the Hazard, Impact, Risk and Vulnerability (HIRV) model and its use of public participation. The paper concludes by offering a synopsis of a case study in the town and regional area surrounding Barriere, British Columbia, Canada. This case study demonstrates a positive outcome when public participation is incorporated into an HIRV analysis.     

Wax perception in honeybees: contact is not necessary

In social insects, much progress has been made in identifying variations in the cuticular signatures of sexes, castes, kin and reproductive status. In contrast to this, we still do not know how the receivers perceive these recognition cues. This study was designed to investigate whether honeybees use contact-chemosensory or olfactory sensilla to perceive wax components. To answer this question in a behavioral assay, we combined classical conditioning of the proboscis extension reaction and a recently established method using zinc sulfate to selectively block antennal contact-chemosensory sensilla. Comparison of the responses to sucrose, wax and geraniol before and after antennal zinc sulfate treatment revealed that the sucrose response is lost after treatment but the responses to wax and geraniol are maintained. As sucrose is perceived by the contact-chemosensory sensilla, the retention of the wax response indicates that contact-chemosensory sensilla are not necessary for wax perception.     

The effect of metapleural gland secretion on the growth of a mutualistic bacterium on the cuticle of leaf-cutting ants

In Acromyrmex octospinosus leaf-cutting ants the metapleural glands produce an array of antibiotic compounds that serve as a general defence against unwanted microbes on the cuticle. Leaf-cutting ants also grow mutualistic Pseudonocardiaceae bacteria on their cuticle that produce antibiotics controlling a microfungal parasite of their fungus gardens. Interaction between this bacterium and gland secretion therefore seems unavoidable. We document the typical development of bacterial growth on the cuticle of young major workers, show that growth starts a few days after eclosion, and that the maximal cover is reached after 2–3 weeks and gradually declines when workers mature. Experimental closure of the metapleural glands had no effect on the initial exponential growth phase of the bacterium, but significantly reduced the cover during the decline phase. The age-dependent abundance of the bacterium and its partial dependence on metapleural gland secretion support the hypothesis that the abundance of this mutualist is actively regulated.     

Codling moth males do not discriminate between pheromone and a pheromone/antagonist blend during upwind flight

Discrimination between conspecific and heterospecific signals is a key element in the evolution of pheromone-mediated communication in insects. Pheromone antagonists prevent heterospecific attraction. They are typically pheromone synergists in other species and enable specific communication in closely related species, using partly the same chemicals. In codling moth, Cydia pomonella, as in other moths, upwind flights to a pheromone/antagonist blend were slower and more convoluted than to pheromone. However, this deteriorated flight behaviour did not account for the strong decrease in male attraction. The pheromone/antagonist blend blocked instead the onset of upwind flight. This was corroborated by placing a separate source of pheromone close by, which restored male attraction. Males flying upwind did not discriminate between pheromone and the adjacent pheromone/antagonist blend, and landed on either source. This indicates differences in the neural regulation for initiating and maintaining a behavioural response to pheromone.     

Transmission of ice-nucleating active bacteria from a prey reduces cold hardiness of a predator (Araneae: Theridiidae)

The influence of ice-nucleating active (INA) bacteria on cold hardiness of the house spider, Achaearanea tepidariorum, was determined by measuring the supercooling point (SCP) of hatchlings given either INA-bacteria-fed or bacteria-free prey (Drosophila melanogaster). Spiders that had eaten INA-bacteria-fed flies showed higher SCPs than those fed on bacteria-free flies. Through feeding, INA bacteria in the prey reduce the cold hardiness of spiders. This fact should be taken into account before using INA agents as a means of pest management.     

Landmark memories are more robust when acquired at the nest site than en route: experiments in desert ants

Foraging desert ants, Cataglyphis fortis, encounter different sequences of visual landmarks while navigating by path integration. This paper explores the question whether the storage of landmark information depends on the context in which the landmarks are learned during an ant's foraging journey. Two experimental set-ups were designed in which the ants experienced an artificial landmark panorama that was placed either around the nest entrance (nest marks) or along the vector route leading straight towards the feeder (route marks). The two training paradigms resulted in pronounced differences in the storage characteristics of the acquired landmark information: memory traces of nest marks were much more robust against extinction and/or suppression than those of route marks. In functional terms, this result is in accord with the observation that desert ants encounter new route marks during every foraging run but always pass the same landmarks when approaching the nest entrance.     

The effect of octopamine on behavioral responses of free-foraging bumblebees to a change in food source profitability

The invertebrate neuromodulator octopamine is known to be involved in bees' associative learning, enhancing the responsiveness of a bee to a conditioned stimulus. In this study, we tested the effect of octopamine on the choice behavior of free-flying bumblebees using a two-phase experiment in an array of artificial flowers. During the first phase of the experiment, the bee was allowed to collect octopamine-laden sugar water from two types of equally rewarding flowers (yellow versus blue). In the second phase, one type of flower was set to be unrewarding. The behavior of the bee (proportion of visits to the unrewarding flowers) over the two phases was fitted to a sigmoid regression model. Our results show that octopamine had no significant effect on the bees' equilibrium choice or on the overall rate of the behavioral change in response to the change in reward. Rather, octopamine significantly affected the time interval between the change in reward status and the initiation of behavioral change in the bee.