Translation of user requirements into house designs: A multi-dimensional scaling analysis

An experiment was conducted to determine how user requirements for single-storey houses are translated into actual design layouts. Architectural students were asked to provide floorplans of houses for four quite different hypothetical users. The briefs contained information either about spatial requirements or about life-style characteristics. Another group of architectural students later evaluated the appropriateness of the designs for the intended user. An INDSCAL analysis of the data (inter-room distances) produced a 4-dimensional solution that accounted for 65% of the variance between house designs. The salience of the dimensions seemed to be more a function of the designer than of the requirements of the user.It was found that when users' requirements are expressed in spatial terms only, the designs produced are clearly a function of the user, not the designer. The more information supplied about the user, the greater the tendency for congruence in the designs. When the brief consisted of life-style characteristics only, the results remained equivocal. There appeared to be little consensus on what designs were appropriate for the hypothetical users.The experiment strongly suggested that (a) architecture students handle information about spatial requirements with ease and with a good deal of consensus on what they consider to be the appropriate design solution for each user; (b) information about life-style characteristics and activity profiles, on the other hand, is handled quite differently by different students. In the latter case, the house designs produced probably follow implicit design-behaviour theories that are quite idiosyncratic. A number of theoretical and methodological issues that require further investigation are raised in the discussion.     

Advanced manufacturing technology and work simplification: An empirical study

This paper is concerned with the effects of advanced manufacturing technology on shopfloor operator jobs and work attitudes. A strong line of argument from labour process theory suggests that such technology will simplify and deskill jobs, and reduce the quality or working life. This proposition was explored within a large electronics company which assembles computer boards, where two different applications of information technology have been experienced. These were compared with two traditional manual assembly jobs. Assessment of job content, perceived job characteristics and operator work attitudes, showed no uniform deskilling effects of advanced manufacturing technologies. Much larger differences existed both between the different new technology applications and between the traditional jobs. This demonstrates that choice of technology is important to skill use and employees' attitudes, as are the choices for the organization of work around it. These are not uniform processes even within a single organization. Experience of operating advanced manufacturing technology was also accompanied by more positive views concerning its impact on the shopfloor.     

Conservation tillage: best management practice for nonpoint runoff

Decisionmakers are in the process of selecting remedial measures for controlling nonpoint pollution runoff. Conservation tillage (CT) is being looked to as one of the major recommended practices. Many different systems exist and vary in the amount of crop residue left and soil roughness produced. Therefore, varying results occur in terms of yield and potential water quality impacts. Differences vary with type of tillage system, soils, geographic region, and the farmer's management. The purpose of this review is to provide material to decisionmakers that points out the assets and liabilities of the various CT systems. Tillage effects on soil characteristics and plant growth are presented and include a discussion of soil moisture and temperature, weed and insect control, nutrient availability, and yields. Water quality aspects are addressed through a discussion of the effects CT systems have on sediment, water, pesticide, and phosphorus loss.This work was supported by the Soil Science Department, College of Agriculture and Life Sciences, University of Wisconsin-Madison, and the U.S. Environmental Protection Agency, Region V, Chicago, Illinois. (Grant No. G005139-01).     

铜与毒死蜱复合污染对淡水绿藻的毒性效应

重金属及农药残留在水环境中被频繁检测出,其复合污染对环境生物的联合毒性有别于单因子的生物效应.以淡水绿藻为受试生物,比较分析了有机磷农药毒死蜱（Chlorpyrifos）和重金属铜的单一及复合暴露对蛋白核小球藻急性毒性、细胞通透性及抗氧化应激的影响.铜、毒死蜱72小时单一暴露对小球藻的EC₅₀分别为0.68和12.71μmol/L,藻细胞叶绿素含量随污染物浓度的增大而降低,细胞通透性随污染物浓度的增大而增强,藻细胞活性氧和抗氧化酶被显著诱导.利用相加指数法（Additive Index,AI）确定铜、毒死蜱联合暴露对小球藻急性毒性的联合作用类型为拮抗作用,这与小球藻ROS产生量及抗氧化酶等指标的显著性水平分析结果一致.     

土壤生态系统微生物多样性技术研究进展

土壤微生物多样性主要研究土壤环境中微生物种群的类别、丰度、分布、结构变化及微生物群落功能的多样性,是土壤生物多样性研究的主体部分。19世纪末,传统的微生物分离培养方法应用于土壤微生物多样性解析。至20世纪70年代,建立了以磷脂脂肪酸图谱分析法(PLFA)和BIOLOG微量分析法为代表的对土壤微生物群落多样性评价的生物化学方法。20世纪90年代后期,随着分子生物学技术的快速发展,建立了变性梯度凝胶电泳(DGGE)、末端限制性片段长度多样性(TRFLP)、克隆文库和高通量测序等土壤微生物多样性研究方法。本文综述了土壤微生物多样性研究技术的原理、进展,并对不同技术的优缺点及应用进行探讨,并对相关领域研究提出思考。     

湖北省山洪灾害暴雨的中尺度分析

湖北省西部是山洪灾害的多发地，暴雨是诱发山洪灾害的直接原因。根据该省气象部门收集的1954—2003年中的226例山洪资料，主要是1980—1987年5—9月该省西部31个气象台站和124个水文站的地面中尺度观测资料，分析了该省山洪灾害的时空分布特征，特别是分析了造成该省两部山洪灾害的中尺度雨团的特征，以及中小心度天气系统演变的规律。     

A Daily Time Series Analysis of StreamWater Phosphorus Concentrations Along an Urban to Forest Gradient

During a 1-year period, we sampled stream water total phosphorus (TP) concentrations daily and soluble reactive phosphorus (SRP) concentrations weekly in four Seattle area streams spanning a gradient of forested to urban-dominated land cover. The objective of this study was to develop time series models describing stream water phosphorus concentration dependence on seasonal variation in stream base flows, short-term flow fluctuations, antecedent flow conditions, and rainfall. Stream water SRP concentrations varied on average by ±18% or ±5.7 μg/L from one week to another, whereas TP varied ±48% or ±32.5 μg/L from one week to another. On average, SRP constituted about 47% of TP. Stream water SRP concentrations followed a simple sine-wave annual cycle with high concentrations during the low-flow summer period and low concentrations during the high-flow winter period in three of the four study sites. These trends are probably due to seasonal variation in the relative contributions of groundwater and subsurface flows to stream flow. In forested Issaquah Creek, SRP concentrations were relatively constant throughout the year except during the fall, when a major salmon spawning run occurred in the stream and SRP concentrations increased markedly. Stream water SRP concentrations were statistically unrelated to short-term flow fluctuations, antecedent flow conditions, or rainfall in each of the study streams. Stream water TP concentrations are highly variable and strongly influenced by short-term flow fluctuations. Each of the processes assessed had statistically significant correlations with TP concentrations, with seasonal base flow being the strongest, followed by antecedent flow conditions, short-term flow fluctuations, and rainfall. Times series models for each individual stream were able to predict ∼70% of the variability in the SRP annual cycle in three of the four streams (r² = 0.57–0.81), whereas individual TP models explained ∼50% of the annual cycle in all streams (r² = 0.39–0.59). Overall, time series models for SRP and TP dynamics explained 82% and 76% of the variability for these variables, respectively. Our results indicate that SRP, the most biologically available and therefore most important phosphorus fraction, has simpler and easier-to-predict seasonal and weekly dynamics.     

充分发挥科技资料在防震减灾事业中的作用

为了充分发挥科技资料在防震减灾事业中的作用,就如何改进地震科技资料的管理方式、提高地震科技资料的管理水平、为防震减灾一线科研人员提供最满意的服务进行了论述.     

Purification and biochemical characterization of recombinant alcohol dehydrogenase from the psychrophilic bacterium <Emphasis Type="Italic">Pseudomonas</Emphasis><Emphasis Type="Italic">frederiksbergensis</Emphasis>

A gram-negative psychrophilic bacterium, with potential for biodegradation of long-chain n-alkanes was isolated from ice samples collected in Spitzbergen, Denmark. On the basis of results of biochemical and morphological tests and sequence analysis of 16S rRNA, the strain was identified as Pseudomonas frederiksbergensis. In this work, a short-chain NAD⁺-dependent alcohol dehydrogenase (alcDH) (Accession number: AAR13804) from the P. frederiksbergensis was cloned and transformed in E. coli BL21 (3DE) competent cells. The alcDH activity was highest in the crude extract of cells induced with 1.0 mM IPTG. The recombinant alcDH enzyme was purified to 93.4% homogeneity using three consecutive purification steps including ammonium sulphate, Q-Sepharose Fast Flow column and gel filtration chromatography employing Superdex 200 10/30 HR column. Enzyme enrichment and yield levels of 31.4 folds and 25.5%, respectively, were achieved. While the subunit molecular mass of the enzyme was determined on SDS-PAGE to be ~38 kDa, the aggregated native form of the enzyme had a molecular mass of ~238 kDa by gel filtration analysis. Reaction conditions optima for the recombinant alcDH were determined with propan-1-ol as the substrate. While the optimum pH was 9, the optimum temperature was 35 °C. The alcDH enzyme exhibited moderate thermal stability with half-lives of 150 min at 55 °C, 27 min at 65 °C and 8 min at 75 °C. Results for kinetic parameters indicated that the apparent K _m value for alcDH with propan-1-ol as the substrate was found to be 1.42 mM and the V _max value was 0.63 mmol mg⁻¹ min⁻¹. Experimental evidence revealed that the recombinant alcDH exhibited a wide range of substrate specificity, with higher levels of specific activity for aliphatic alcohols as compared to secondary alcohols. Taken together, the present study highlights the potential of alcDH as a member of cold-adapted enzymes in several key biotechnological applications including environmental bioremediation and biotransformations. It is envisaged that, with the ongoing screening of microorganisms and metagenomes, directed evolution approaches and the subsequent overexpression of recombinant proteins, more enzymes will be found that are suitable for bioremediation purposes.