Mutagenic and cytotoxic potential of Endosulfan and Lambda-cyhalothrin - In vitro study describing individual and combined effects of pesticides

Excessive use of pesticides poses increased risks to non target species including humans. In the developing countries, lack of proper awareness about the toxic potential of pesticides makes the farmer more vulnerable to pesticide linked toxicities, which could lead to diverse pathological conditions. The toxic potential of a pesticide could be determined by their ability to induce genetic mutations and cytotoxicity. Hence, determination of genetic mutation and cytotoxicity of each pesticide is unavoidable to legislate health and safety appraisal about pesticides. The objective of current investigation was to determine the genotoxic and cytotoxic potential of Endosulfan（EN） and Lambda-cyhalothrin（LC）; individually and in combination. 3-（4,5-Dimethylthiazol-2-yl）-2,5-Diphenyltetrazolium Bromide（MTT） assay was utilized to determine cytotoxicity, while two mutant histidine dependent Salmonella strains（TA98, TA100） were used to determine the mutagenicity of EN and LC.Moreover, mutagenicity assay was conducted with and without S9 to evaluate the effects of metabolic activation on mutagenicity. Even though a dose dependent increase in the number of revertant colonies was detected with EN against both bacterial strains, a highly significant（p 〈 0.05） increase in the mutagenicity was detected in TA98 with S9. In comparison, data obtained from LC revealed less mutagenic potential than EN. Surprisingly,the non-mutagenic individual-concentrations of EN and LC showed dose dependent mutagenicity when combined. Combination of EN and LC synergistically induced mutagenicity both in TA98 and TA100. MTT assay spotlighted comparable dose dependent cytotoxicity effects of both pesticides. Interestingly, the combination of EN and LC produced increased reversion and cytotoxicity at lower doses as compared to each pesticide, concluding that pesticide exposure even at sub-lethal doses can produce cytotoxicity and genetic mutations, which could lead to carcinogenicity.     

Impact of climate change: an empirical investigation of Malaysian rice production

This paper examines the possible impact of climate change on Malaysian rice production. Using the Ricardian Method and farm household data, we have examined the effects on rice producers focusing on sharecropper adaptations and ecological causes. The principle goal of this research paper is to investigate the impact of climate variables such as temperature, rainfall and precipitation on rice production during main and off seasons, in Kedah, Malaysia. The main purpose of this paper is to examine how climate change affects the net income of paddy farmers in the study area. The statistically significant results show that temperature, rainfall, farm size, educational knowledge, land area and value of labour input have an impact on rice production per hectare revealing potential impacts of climate vulnerability on Malaysian agriculture. The results showed that net revenue increased by Ringgit Malaysia (RM 1= $0.3277) 4.78 per hectare throughout the main seasons as a result of minimal increase in the temperature while there is a decrease of RM 3.02 in the net revenue per hectare during off seasons. During the off season, rainfall increased revenue per hectare by RM 1.32 and during the main season it reduced revenue per hectare by RM 1.01. The evidence from this study may be useful for Malaysian policymakers to facilitate greater preventive measures during the main and off-seasons to counteract climate uncertainty and vulnerability.     

Health risks of heavy metals in contaminated soils and food crops irrigated with wastewater in Beijing, China

Consumption of food crops contaminated with heavy metals is a major food chain route for human exposure. We studied the health risks of heavy metals in contaminated food crops irrigated with wastewater. Results indicate that there is a substantial buildup of heavy metals in wastewater-irrigated soils, collected from Beijing, China. Heavy metal concentrations in plants grown in wastewater-irrigated soils were significantly higher (P相似文献   

Extent and causes of 3D spatial variations in potential N mineralization and the risk of ammonium and nitrate leaching from an N-impacted permanent grassland near York, UK

Changes in the dynamics of inorganic N species transformations with depth have been investigated for seven soil profiles from a nitrogen-impacted ancient grassland on a nature reserve outside York in the UK, using incubation experiments. In five of the profiles, both ammonification and nitrification are occurring below the rooting zone, probably partly in response to the low C:N ratio in the soils. This contributes to elevated nitrate concentrations found in an adjacent stream. Accumulation of ammonium during incubation in the sub-soils of these five profiles suggests a high probability of ammonium leaching down the profiles as ammonium inputs and outputs at a given depth approach equilibrium. This ammonium may also be nitrified at depth. However, in the two profiles with the most acidic surface horizons, net mineralization was negligible or negative; some initial ammonium-N and ammonium-N produced during incubation were nitrified, so the loss in ammonium-N was closely balanced by nitrate-N production.     

A comparison of acute toxicity of biodiesel, biodiesel blends, and diesel on aquatic organisms

The increased demand of alternative energy sources has created interest in biodiesel and biodiesel blends; biodiesel is promoted as a diesel substitute that is safer, produces less harmful combustion emissions, and biodegrades more easily. Like diesel spills, biodiesel can have deleterious effects on the aquatic environments. The effect of neat biodiesel, biodiesel blends, and diesel on Oncorhynchus mykiss and Daphnia magna was evaluated using acute toxicity testing. Static nonrenewal bioassays of freshwater organisms containing B100, B50, B20, B5, and conventional diesel fuel were used to compare the acute effects of biodiesel to diesel. Mortality was the significant end point measured in this study; percent mortality and lethal concentration (LC50) at different exposure times were determined from the acute toxicity tests performed. Trials were considered valid if the controls exhibited > 90% survival. Based on percentage of mortality and LC50 values, a toxicity ranking of fuels was developed.     

A two-generational reproductive toxicity study of zinc in rats

A two-generation reproductive toxicity study of zinc chloride (ZnCl(2)) was conducted in rats. F(o) male and female rats were administered 0.00 (control), 7.50 (low), 15.00 (mid) and 30.00 (high) mg/kg/day of ZnCl(2). Selected F(1) male and female rats were exposed to the same doses received by their parents (F(o)). Exposure of F(0) parental rats to ZnCl(2) showed significant reduction in fertility, viability (days 0 and 4), and the body weight of F(1) pups from the high-dose group but caused no effects on litter size, weaning index, and sex ratio. Similarly, the continued exposure of F(1) parental rats to ZnCl(2) also reduced fertility, liter size, viability (day 0), and the body weight of F(2) pups within the high-dose group but caused no effects on weaning index and sex ratio. Exposure of ZnCl(2) to F(0) and F(1) parental males resulted in a significant reduction in their body weights, and the F(0) and F(1) parental females did not show any significant difference in their body weights compared to their control groups. However, the postpartum dam weights of both F(0) and F(1) female rats were significantly reduced compared to their controls. Exposure of ZnCl(2) to F(o) and F(1) generation parental rats did not produce any significant change of their clinical signs as well as their clinical pathology parameters, except the alkaline phosphotase (ALK) level, which showed an upward trend in both sexes of both generations. Exposure of ZnCl(2) to F(0) rats resulted in a reduction of brain, liver, kidney, spleen and seminal vesicles weights of males and in the spleen and uterus of females. Similarly, exposure of F(1) rats to ZnCl(2) also resulted in reduction of brain, liver, kidney, adrenal, spleen, prostate and seminal vesicles weights of males and in spleen and uterus of females. ZnCl(2) exposure resulted in grossly observed gastro-intestianla (GI) tract, lymphoreticular/hematopoietic, and reproductive tract lesions in parental rats in both generations. Reduced body fat was also recorded in F(1) parental rats.     

Temporal change in the distribution patterns of hexachlorobenzene and dichlorodiphenyltrichloroethane among various soil organic matter fractions

Residence time-dependent distribution patterns of hexachlorobenzene (HCB) and dichlorodiphenyltrichloroethane (DDT) among different soil organic matter fractions of three Chinese soils were investigated. Soil organic matter (SOM) was fractionated into fulvic acid (FA), humic acid (HA), bound-humic acid (BHA), lipid, and insoluble residue (IR) fractions using methyl isobutyl ketone (MIBK) method. Results revealed that as the residence time prolonged, the amounts of HCB and DDT in the FA, HA and BHA fractions decreased, while those in the lipid and IR fractions increased. One- and two-compartment first order, and one- and two-parameter pore-diffusion kinetic models were used to describe the mobility of HCB and DDT from the FA, HA and BHA fractions. The results suggest that excellent agreements were achieved between the experimental data and fits to the two-compartment first order kinetic model (R2>0.97). The transfer rates of HCB and DDT followed the order FA>HA>BHA.     

Effect of metal tolerant plant growth promoting Bradyrhizobium sp. (vigna) on growth, symbiosis, seed yield and metal uptake by greengram plants

The nickel and zinc tolerant plant growth promoting Bradyrhizobium sp. (vigna) RM8 was isolated from nodules of greengram, grown in metal contaminated Indian soils. The plant growth promoting (PGP) potentials of strain RM8 was assessed both in the presence and absence of nickel and zinc under in vitro conditions. Strain RM8 tolerated a high level of nickel (300 microg ml(-1)) and zinc (1400 microg ml(-1)) on yeast extract mannitol agar medium. Bradyrhizobium sp. (vigna) strain RM8 produced 13.3 microg ml(-1) of indole acetic acid in Luria Bertani broth at 100 microg ml(-1) of tryptophan which increased to 13.6 microg ml(-1) at 50 microg Ni ml(-1) and 13.5 microg ml(-1) at 300 microg Zn ml(-1). Strain RM8 was positive for siderophore, HCN and ammonia both in the absence and presence of nickel and zinc. The PGP activity of this strain was further evaluated with increasing concentrations of nickel and zinc using greengram as a test crop. The bioinoculant enhanced the nodule numbers by 82%, leghaemoglobin by 120%, seed yield by 34%, grain protein by 13%, root N by 41% and shoot N by 37% at 290 mg Ni kg(-1) soil. At 4890 mg Zn kg(-1) soil, the bioinoculant increased the nodule numbers by 50%, leghaemoglobin by 100%, seed yield by 36%, grain protein by 13%, root N by 47% and shoot N by 42%. The bioinoculant strain RM8 reduced the uptake of nickel and zinc by plant organs compared to plants grown in the absence of bioinoculant. This study suggested that the bioinoculant due to its intrinsic abilities of growth promotion and attenuation of the toxic effects of nickel and zinc could be exploited for remediation of metal from nickel and zinc contaminated sites.     

Application of a “closed-can” technique for measuring radon exhalation from mine samples of Punjab, Pakistan

Using the closed-can technique, radon exhalation rate measurements have been carried out for shale and coal samples collected from various mines located in the Chakwal and Makarwal areas of Pakistan. For the two areas, the measured average values of the exhalation rates from shale are 1.45±0.13 and 0.67±0.25 Bq m⁻² h⁻¹ and for coal are 1.0±0.03 and 0.65±0.32 Bq m⁻² h⁻¹, respectively. These values are much lower than the measured exhalation rates from alum-shale-based Nordic concrete which has values in the 50–200 Bq m⁻² h⁻¹ range. The lower values of the measured exhalation rates from the shale and coal deposits in the Chakwal and Makarwal areas are indicative of their lower uranium contents and mine workers in these areas do not face any abnormal health hazard due to radon since the exhalation rates have been found to be on the low side.     

Renewable energy in Australia: a wider policy discourse

ABSTRACT

This paper assesses the evolution of generation technology-mix in Australia, with specific emphasis on understanding how such evolution has been shaped by wider political and socio-economic influences. This assessment is predicated on the argument that the contemporary, quintessentially techno-economic, policy discourse on renewable energy is deficient, as it ignores climacteric political and socio-economic influences on generation technology-mix. The methodological framework employed in this paper is informed by the core tenets of technological change theory. The assessment suggests that generation technology-mix in Australia has historically been overwhelmingly influenced by the underlying technological paradigm of the electricity industry; and that this technological paradigm essentially draws its imprimatur from the wider political and socio-economic contexts. By implication, it suggests that a rapid uptake of renewables will have widespread ramifications, extending into political, socio-economic and cultural realms of a society. Clearly, existing policy discourse – that tends to focus on technical potentials, cost competitiveness, externalities and risks of various renewable technologies – is deficient. A much broader discourse is needed. This paper also made an attempt to develop a basis for such a discourse by reviewing broader aspects of the Australian society that would be affected by a rapid uptake of renewables.