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171.
MW Nonnenmann G Coronado B Thompson WC Griffith JD Hanson S Vesper EM Faustman 《Journal of environmental monitoring : JEM》2012,14(8):2038-2043
Molecular techniques are an alternative to culturing and counting methods in quantifying indoor fungal contamination. Pyrosequencing offers the possibility of identifying unexpected indoor fungi. In this study, 50 house dust samples were collected from homes in the Yakima Valley, WA. Each sample was analyzed by quantitative PCR (QPCR) for 36 common fungi and by fungal tag-encoded flexible (FLX) amplicon pyrosequencing (fTEFAP) for these and additional fungi. Only 24 of the samples yielded amplified results using fTEFAP but QPCR successfully amplified all 50 samples. Over 450 fungal species were detected by fTEFAP but most were rare. Twenty-two fungi were found by fTEFAP to occur with at least an average of ≥0.5% relative occurrence. Many of these fungi seem to be associated with plants, soil or human skin. Combining fTEFAP and QPCR can enhance studies of fungal contamination in homes. 相似文献
172.
Singh HP Sharma VP Batish DR Kohli RK 《Environmental monitoring and assessment》2012,184(4):1813-1821
Indiscriminate adoption and use of cell phone technology has tremendously increased the levels of electromagnetic field radiations
(EMFr) in the natural environment. It has raised the concerns among the scientists regarding the possible risks of EMFr to
living organisms. However, not much has been done to assess the damage caused to plants that are continuously exposed to EMFr
present in the environment. The present study investigated the biochemical mechanism of interference of 900 MHz cell phone
EMFr with root formation in mung bean (Vigna radiata syn. Phaseolus aureus) hypocotyls, a model system to study rhizogenesis in plants. Cell phone EMFr enhanced the activities of proteases (by 1.52
to 2.33 times), polyphenol oxidases (by 1.5 to 4.3 times), and peroxidases (by 1.5 to 2.0 times) in mung bean hypocotyls over
control. Further, EMFr enhanced malondialdehyde (an indicator of lipid peroxidation), hydrogen peroxide, and proline content,
indicating a reactive oxygen species-mediated oxidative damage in hypocotyls. It was confirmed by the upregulation in the
activities of antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, catalase, and glutathione
reductase) suggesting their possible role in providing protection against EMFr-induced oxidative damage. The study concluded
that cell phone radiations affect the process of rhizogenesis through biochemical alterations that manifest as oxidative damage
resulting in root impairment. 相似文献
173.
Chaudhary P Singh SB Chaudhry S Nain L 《Environmental monitoring and assessment》2012,184(2):1145-1156
The present study is aimed at analysing and comparing different soil enzymes in soil samples of native contaminated sites
of a Mathura refinery and adjoining agricultural land. Enzyme activities are considered as indicators of soil quality and
changes in biogeochemical function due to management or perturbations. Soil samples were collected from the premises and nearby
area of Mathura refinery, India. Biological health parameters (dehydrogenase, aryl esterase, aryl sulphatase,
\upbeta \upbeta -glucosidase, alkaline phosphatase, acid phosphatase, lipase, laccase and catalase activity) were estimated in the soil samples.
Among all the samples, sewage sludge soil showed maximum activity of enzymes, microbial biomass carbon and most probable number
of polycyclic aromatic hydrocarbon (PAH) degraders in soils spiked with three- to four-ring PAHs at 50 ppm. Available phosphorus,
potassium and nitrogen was also exceptionally high in this sample, indicating maximum microbial bioconversion due to presence
of nutrients stimulating potent PAH-degrading microorganisms. 相似文献
174.
The technique of diffusion gradient in thin films (DGT) for assessing bioavailable metals has not been tested under field
conditions. We assessed the relationships of DGT- and cation exchange resin-membrane-measured concentrations of Cd, Cu, Pb,
and Zn with plant uptake of the metals under greenhouse and field conditions. In the greenhouse, the effective concentrations
of Cu, Pb, and Zn by DGT correlated significantly with uptake by sorghum (Sorghum bicolor), but cation exchange resin-membrane-measured concentrations of Cd, Pb, and Zn did not correlate with sorghum uptake. In
the field, the DGT-measured concentrations of Cd, Pb, and Zn were not linearly related to uptake Cd, Pb, and Zn by lettuce
(Lactuca sativa) except for Cu uptake (r = 0.87, p < 0.05). Similarly, it was only the resin-membrane-extractable Pb that correlated with Pb uptake by lettuce (r = 0.77; p < 0.05). However, fitting non-linear regression models improved the plant metal uptake predictions by DGT-measured bioavailable
Cd, Cu, Pb, and Zn under field conditions. In conclusion, the DGT technique was fairly predictive of bioavailability in the
greenhouse, but not in the field. 相似文献
175.
Sediment quality guidelines (SQGs) can be derived using different approaches and are commonly used in environmental management,
reclamation, and risk assessment. The screening-level concentration (SLC) approach has been used in Ontario, Canada, to derive
lowest effect levels (LELs) and severe effect levels for use as SQGs. This approach was adopted by the Canadian Nuclear Safety
Commission (CNSC) to set guidelines for metals (As, Cr, Cu, Pb, Mo, Ni, Se, U, and V) and radionuclides (Ra-226, Pb-210, and
Po-210) in sediment at northern Saskatchewan uranium mining and milling operations. The SLC approach is based on total metal
and radionuclide concentrations in sediment, and corresponding benthic community composition data for a specific sampling
site. In this study, sediment chemistry (total metals and radionuclides) and benthic community data from northern Saskatchewan
uranium operations were compiled and examined. Results indicate that the CNSC-derived SQGs had limited relationships to observed
effects, or lack thereof, on benthic invertebrate communities near uranium operations in Saskatchewan. The LELs were found
to correctly align with effects at 95% of the sites that had effects, on a general basis, but on an element-specific basis
many of the elements had concentrations at effect sites below their LELs. Furthermore, concentrations of the evaluated elements
exceeded at least one LEL at 60% of the no-effect sites. The high number of exceedences of LELs at reference and no-effect
sites (false-positives) calls to question the appropriateness of the CNSC-derived SQGs. It is suggested that alternatives
to the SLC approach be explored. 相似文献
176.
A study of chemical and sedimentological parameters integrated with benthic foraminifera investigation was conducted along
the northern coast of Gabes Gulf. Thirty-two samples were studied and a total of 68 benthic foraminiferal species were identified.
Heavy metals enrichment factors and total hydrocarbon concentrations showed both metal and petrogenic pollution related mainly
to phosphogypsum, sewage, and fishing activities. Statistical analysis (bivariate correlation and hierarchical cluster analysis)
show a possible control of these pollutants on density, diversity, as well as the taxonomic composition of the benthic foraminiferal
assemblages. The extent to which the population was found less dense and less diversified corresponded to the degree to which
the sediment was contaminated. In these contaminated sites, an increase in relative abundance of opportunistic species such
Ammonia tepida and Haynesina germanica was found. Far from pollution, foraminiferal assemblages are dominated by species characteristic of Mediterranean shallow
water (Ammonia beccarii, Ammonia parkinsoniana, Elphidium crispum, Elphidium williamsoni, Elphidium advenum, Peneroplis planatus, Peneroplis pertesus). 相似文献
177.
Brázová T Hanzelová V Miklisová D Salgovičová D Turčeková L 《Environmental monitoring and assessment》2012,184(11):6553-6561
The distribution and concentrations of polychlorinated biphenyls (PCBs) were determined in fish species (European perch Perca fluviatilis, northern pike Esox lucius, pike perch Sander lucioperca, wels catfish Silirus glanus, common carp Cyprinus carpio, European eel Anguilla anguilla, freshwater bream Abramis brama, goldfish Carassius auratus, and roach Rutilus rutilus) in a heavily polluted water reservoir Zemplínska ?írava (Slovakia). The study performed at two different time points 5?years apart (2004 and 2009) revealed serious PCB contamination of fish muscle tissue and significant interspecies as well as tissue-specific differences in PCB uptake by fish. Total PCBs broadly correlated with the trophic position of individual fish species within a food chain (P?0.01). The concentrations were particularly high in predatory fish species, perch, pike, and pike perch (108.0, 90.1, and 113.0?mg?kg(-1) lipid wt, respectively), but comparable PCB values were also found in non-predatory detrivorous freshwater bream (128.0?mg?kg(-1) lipid wt). The lowest PCB values were surprisingly assessed in European eel (17.1?mg?kg(-1) lipid wt). Tissue analysis showed the highest storage capacity of the liver (hepatopancreas in cyprinids) with maximum concentrations recorded found in northern pike (214.0?mg?kg(-1) lipid wt) and freshwater bream (163.0?mg?kg(-1) lipid wt). Negative correlations, mostly not significant, between the total PCB concentrations and fish weight were observed (P?>?0.05). The study has shown that the kind of fish, its feeding habit, and specific conditions of the habitat are mutually interrelated factors that are responsible for significant variations in fish body burdens. A tendency to PCB biomagnification was also proved in some fish species of this water reservoir. 相似文献
178.
Highways and main roads are a potential source of contamination for the surrounding environment. High traffic rates result in elevated heavy metal concentrations in road runoff, soil and water seepage, which has attracted much attention in the recent past. Nonetheless, investigations of pollutants in roadside soils are still a subject of major interest due to the rapid development of traffic systems and increasing traffic all over the world. The accumulation of the heavy metals Pb, Cd, Cu and Zn in soils along the oldest federal highway of the world has been studied by sampling a roadside transect of 125 by 10?m. In addition, heavy metal concentrations of Pb, Cd, Zn, Cu, Ni and Cr in soil solutions from different distances (2.5, 5 and 10?m) from the hard shoulder of the highway and from three soil depths (10, 30, and 50?cm) were investigated. The results show that heavy metal concentrations are up to 20 times increased compared to the geochemical background levels and a reference site of 800-m distance from the roadside. Soil matrix concentrations in the topsoil (0-10?cm) mostly exceeded the precautionary values of the German Federal Soil Protection and Contamination Ordinance (BBodSchV). The concentrations of Cd, Pb and Zn in the soil matrix tended to decrease with distance from the roadside edge, whereas the concentrations in the soil solution increased at a distance of 10?m onwards due to a lower soil pH. Because of both high pH values and a high sorption capacity of the soils, soil solution concentrations seldom exceeded the trigger values of the German Federal Soil Protection and Contamination Ordinance (BBodSchV) for transferring soil solution to groundwater. 相似文献
179.
Multivariate analysis of mixed contaminants (PAHs and heavy metals) at manufactured gas plant site soils 总被引:1,自引:0,他引:1
Principal component analysis (PCA) was used to provide an overview of the distribution pattern of polycyclic aromatic hydrocarbons
(PAHs) and heavy metals in former manufactured gas plant (MGP) site soils. PCA is the powerful multivariate method to identify
the patterns in data and expressing their similarities and differences. Ten PAHs (naphthalene, acenapthylene, acenaphthene,
fluorene, phenanthrene, anthracene, fluoranthene, pyrene, chrysene, benzo[a]pyrene) and four toxic heavy metals — lead (Pb), cadmium (Cd), chromium (Cr) and zinc (Zn) — were detected in the site soils.
PAH contamination was contributed equally by both low and high molecular weight PAHs. PCA was performed using the varimax
rotation method in SPSS, 17.0. Two principal components accounting for 91.7% of the total variance was retained using scree
test. Principle component 1 (PC1) substantially explained the dominance of PAH contamination in the MGP site soils. All PAHs,
except anthracene, were positively correlated in PC1. There was a common thread in high molecular weight PAHs loadings, where
the loadings were inversely proportional to the hydrophobicity and molecular weight of individual PAHs. Anthracene, which
was less correlated with other individual PAHs, deviated well from the origin which can be ascribed to its lower toxicity
and different origin than its isomer phenanthrene. Among the four major heavy metals studied in MGP sites, Pb, Cd and Cr were
negatively correlated in PC1 but showed strong positive correlation in principle component 2 (PC2). Although metals may not
have originated directly from gaswork processes, the correlation between PAHs and metals suggests that the materials used
in these sites may have contributed to high concentrations of Pb, Cd, Cr and Zn. Thus, multivariate analysis helped to identify
the sources of PAHs, heavy metals and their association in MGP site, and thereby better characterise the site risk, which
would not be possible if one uses chemical analysis alone. 相似文献
180.