Incorporation and utilization of bacterial lipids in theSolemya velum symbiosis

We undertook a detailed analysis of the lipid composition ofSolemya velum (Say), a bivalve containing endosymbiotic chemoautotrophic bacteria, in order to determine the presence of lipid biomarkers of endosymbiont activity. The symbiont-free clamMya arenaria (L.) and the sulfur-oxidizing bacteriumThiomicrospira crunogena (Jannasch et al.) were analyzed for comparative purposes. The ¹³C ratios of the fatty acids and sterols were also measured to elucidate potential carbon sources for the lipids of each bivalve species. Both fatty acid and sterol composition differed markedly between the two bivalves. The lipids ofS. velum were characterized by large amounts of 18: 17 (cis-vaccenic acid), 16:0, and 16 : 17 fatty acids, and low concentrations of the highly unsaturated plant-derived fatty acids characteristic of most marine bivalves. Cholest-5-en-3-ol (cholesterol) accounted for greater than 95% of the sterols inS. velum. In contrast,M. arenaria had fatty acid and sterol compositions similar to typical marine bivalves and was characterized by large amounts of the highly unsaturated fatty acids 20 : 53 and 22 : 63 and a variety of plant-derived sterols. The fatty acids ofT. crunogena were similar to those ofS. velum and were dominated by 18:17, 16:0 and 16:17 fatty acids. Thecis-vaccenic acid found inS. velum is almost certainly symbiontderived and serves as a potential biomarker for symbiontlipid incorporation by the host. The high concentrations ofcis-vaccenic acid (up to 35% of the total fatty acid content) in both symbiont-containing and symbiont-free tissues ofS. velum demonstrate the importance of the endosymbionts in the lipid metabolism of this bivalve. The presence ofcis-vaccenic acid in all the major lipid classes ofS. velum demonstrates both incorporation and utilization of this compound. The ¹³C ratios of the fatty acids and sterols ofS. velum were significantly lighter (–38.4 to –45.3) than those ofM. arenaria (–23.8 to – 24.2) and were similar to the values found for the fatty acids ofT. crunogena (–45); this suggests that the lipids ofS. velum are either derived directly from the endosymbionts or are synthesized using endosymbiontderived carbon.Woods Hole Oceanographic Institution Contribution No. 7356Please address all correspondence and reprint requests to Dr Conway at her present address: Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260, USA     

Mangrove forest structure and productivity in the Fly River estuary,Papua New Guinea

In April, July and August 1989 and February 1990, the delta region of the Fly River was surveyed to establish the aerial extent of mangrove forests, their species composition, tree densities and basal areas, and potential net primary production. Mangrove forests cover 87 400 ha, mainly on islands within the delta. Twentynine mangrove plant species were recorded, but there were only three major forest types in the delta.Rhizophora apiculata-Bruguiera parviflora forests (hereafterRhizophora-Bruguiera forests) predominated in regions where river water salinities were >10. These forests covered 31 500 ha and had mean total tree densities and basal areas of 2027 stems ha^–1 and 21 m² ha^–1, respectively. Forests of the palmNypa fruticans (hereafterNypa forests) covered 38 400 ha, mainly in regions where river salinities were ~1 to 10, and had mean total densities and basal areas of 4431 stems ha^–1 and 38 m² ha^–1, respectively. Forests dominated byAvicennia marina and/orSonneratia lanceolata (hereafterAvicennia-Sonneratia forests) predominated on accreting banks of sediment and covered 17 500 ha. In very low-salinity (< 1) regions there are large monospecific stands ofS. lanceolata. Mean total densities and basal areas forAvicennia-Sonneratia forests were 7036 stems ha^–1 and 22 m² ha^–1, respectively. Mean net primary productivity (kg C ha^–1 d^–1) was estimated to be 26.7, 27.1 and 19.0 forRhizophora-Bruguiera, Nypa andAvicennia-Sonneratia forests, respectively. Total daily net primary production by all mangrove forests was estimated at 2214 t carbon. Using assumptions based on work in tropical Australia, it was estimated that ~678 t carbon (or 31% of primary production) were exported daily from mangrove forests to the waters of the delta.Contribution No. 550 from the Australian Institute of Marine Science     

Temperature and metabolic rate in sedentary fish from the Antarctic,North Sea and Indo-West Pacific Ocean

Resting metabolic rate was measured in demersal stages of the teleostNotothenia neglecta Nybelin from the South Orkney Islands, Antarctica, from 1985 to 1987. The relationship between and body mass (Mb) conformed to the general relationship , wherea is a proportionality constant andb is the scaling exponent. (mg O₂ h^–1) was found to scale toMb ^{(0.82±0.011)} in the summer (November to April, 1.6 to 1 850 g,n=56) and toMb ^{(0.76±0.013)} in the winter (May to October, 0.9 to 1 850 g,n=57) (values ofb are means ± SD). Although the scaling exponents were significantly different (P<0.01), was similar in the juvenile stages of summer- and winter-caught fish matched for body mass. The effects of activity on oxygen consumption was studied using a Brett respirometer. Adult stages had a factorial aerobic scope for activity of 5.7, which is similar to that reported for demersal fish from temperate latitudes. The effects of temperature on resting metabolism was investigated in fish with similar sedentary lifestyles from the North Sea (Agonus cataphractus andMyoxocephalus scorpius) and the Indo-West Pacific (Paracirrhites forsteri, P. arcatus, Neocirrhites armatus andExallias brevis). Extrapolated values of for the tropical species approached zero at 5 to 10°C. For a standard 50 g fish, for the tropical species at 25°C was in the range 3.4 to 4.4 mg O₂ h^–1, compared with 1.3 mg O₂ h^–1 forNotothenia neglecta at its acclimation temperature. Thus, the maximum metabolic rate of sedentary tropical species at 24°C is likely to be 2 to 4 times higher than inN. neglecta at 0°C. This suggests that the energy available for sustained activity is significantly lower in cold- than in warm-water fish.     

Radionuclides and the birds at Ravenglass

Since 1983, concern has been expressed about the apparent decline in numbers of birds in the Ravenglass estuary in west Cumbria, particularly of the black-headed gull colony on the Drigg dunes, and suggestions have been made that this decline might be due to excessive radiation in the birds' food and their general environment. Twelve species of marine invertebrates from Ravenglass, most of them known to be important foods for birds, were analysed, and further samples were taken from sites along the west Cumbrian coast. None of these samples showed excessive contamination with any of the radionuclides analysed. Analysis of a sample of bird carcasses from the areas showed oystercatchers (Haematopus ostralegus) and shelduck (Tadorna tadorna) to have some of the highest concentrations of (137)Cs in their tissues; yet their breeding success and populations were not affected. Black-headed gulls, on the other hand, were found to be feeding mainly inland, and were the least contaminated with radionuclides of all the birds at Ravenglass, yet this species and its breeding success were in decline. Calculations of the total dose equivalent rate to the whole body of the most contaminated black-headed gull amounted to 9.8 x 10(-4) mSvh(-1) (approximately equal to 8.4 x 10(-4) mGy h(-1), whole body absorbed dose rate), and the background exposure dose was of the order of 8.3 x 10(-4) mGy h(-1). As a minimum chronic dose of 1000 mGy day(-1) has been found necessary to retard growth of nestling birds, and 9600 mGy over 20 days of incubation to cause the death of 50% of embryos in black-headed gulls' eggs, the concentrations of radionuclides in the foods, body tissues and general environment were at least three orders of magnitude too low to have had any effect. The more likely cause of the desertion of the gullery was the combination of an uncontrolled fox population, the severest outbreak of myxomatosis amongst the rabbits since 1954 and the driest May-July period on record, all in the same year (1984).     

Dry aerial fallout of organochlorine insecticide residues in Delhi, India

Monitoring of airborne dust in Delhi during May to July 1985 revealed residues of DDT varying from 1.3 to 7.14 ng mg(-1) (4.06-22.31 ng m(-2) day(-1)) with an average of 3.32 ng mg(-1) (10.38 ng m(-2) day(-1)), and HCH which ranged from 0.46 to 2.35 ng mg(-1) (1.44-7.34 ng m(-2) day(-1)) with a mean of 1.16 ng mg(-1) (3.63 ng m(-2) day(-1)). The concentration of total DDT was almost 3 times greater than that of HCH.     

Studies on the effects of some organic pollutants on the heavy metal transport in an Indian soil

The translocation of some heavy metals, such as Hg, Cd, Cu, Co, Ni and Zn, as affected by organic pollutants, i.e. methanol, ethanol, propanol, formaldehyde, acetaldehyde, benzaldehyde, acetone, ethyl methyl ketone and cyclo-hexanone, was studied in an Indian red soil using soil thin layer chromatography. It was observed that an increase in the concentration of organic compounds in developer enhances the heavy metal mobility, except in the case of Cu and Hg which show a decreasing trend. The results are discussed in relation to the physico-chemical characteristics of the soil and adsorption/desorption phenomena.     

Black cherry growth response to ambient ozone and EDU

Field-grown black cherry (Prunus serotina Ehrh.) seedlings were treated with the antioxidant ethylenediurea (EDU) to evaluate height, diameter, and above-ground dry-weight biomass growth response to ambient ozone over four years. Nine blocks with 44 trees/block were used in a randomized complete block design with three foliar spray treatments: (1) 1000 ppm EDU mixed with a surfactant and water; (2) surfactant mixed with water; and (3) water only. In each growing season treatments were applied seven times at approximately 10-day intervals. Repeated measures analysis of variance indicated significant (P< or =0.05) treatment and year effects for log-transformed height and diameter growth over the four-year period. After four years, EDU-treated trees were approximately 17% taller and stem diameters were 21% greater than non-EDU-treated trees. Total above-ground dry-weight biomass at the end of four years was 47% greater for EDU-treated trees compared to non-EDU-treated trees.     

Digestive mechanics and gluttonous feeding in the feather starOligometra serripinna (Echinodermata: Crinoidea)

The movement and digestion of food in the gut ofOligometra serripinna (Carpenter) were studied at Lizard Island (14°3842S; 145°2710E) in the austral winter of 1986. Feather stars in the laboratory were fed a brief, small meal of brine shrimp nauplii and killed at increasing time intervals thereafter. Histological reconstructions showed that the ingested nauplii progressed along the digestive tract surprisingly quickly. Some nauplii were found in the mid and hind intestine in only 30 min, and all of the nauplii had reached the hind intestine and rectum in 1 h. Digestion of the nauplii had started at 1 h, and only a few fragments of naupliar exoskeleton remained in the hind intestine and rectum 5 h after the start of feeding. Videotape analysis showed that no fecal pellets were released during this experiment. In the natural environment ofO. serripinna, ingested particles may similarly be transported quickly to the hind part of the gut and digested there — when feather stars were fixed in the field, most of the gut contents were found in the hind intestine and rectum.O. serripinna, which efficiently rejects inert particles before they are ingested, usually defecates infrequently (probably not more than once over a span of many hours) and differs from some other feather stars that ingest numerous inert particles and defecate much more frequently. When specimens ofO. serripinna were fed continuously on brine shrimp nauplii,Artemia sp. (San Francisco strain), in the laboratory, the feather stars fed gluttonously, packing their guts with several hundred nauplii in 1 to 2 h. Thereafter, superfluous feeding began (i.e., further ingestions appeared to force undigested nauplii, some of them still living, out of the anus). These observations suggest thatO. serripinna usually feeds at relatively modest rates in its natural habitat, but can feed gluttonously to take advantage of infrequent patches of highly concentrated, nutritious particles (e.g. copepod swarms, migrating demersal zooplankton, and invertebrate gametes from mass spawnings). It is likely that such patches of nutritious particles are usually small enough to drift out of reach of the feather stars before gluttonous feeding proceeds to superfluous feeding. Opportunities for superfluous feeding in nature are probably very infrequent (e.g. ingestion of coral gametes and embryos after a mass spawning), and the feather stars evidently have no behavior that stops further ingestions after the gut becomes filled to capacity.     

The effect of chronic toxicity of copper on the activity of Balbiani rings and nucleolar organizing region in the salivary gland chromosomes of Chironomus ninevah larvae

The effect of copper on the activity of Balbiani rings (BR1 and BR2) and nucleolar organizing region (NOR) in chromosome IV of the salivary gland of the 4th instar larvae of Chironomus ninevah has been investigated. Sublethal concentrations, i.e. 0.02, 0.1, 0.15 and 0.2 mg litre(-1) suppress the activity of BR1, and are statistically significant. The same concentrations reduced the activity of BR2 and NOR, but without significant differences, except 0.1 mg litre(-1) which was significant. The purpose of this work was to evaluate the effect of chronic toxicity of Cu(2+) on the expression of gene activity of midge salivary gland chromosomes Although there may be many more aquatic organisms that are more sensitive to copper, this midge was selected as a biological indicator species because of its association with benthic sediments.     

The influence of acidic mine and spoil drainage on water quality in the mid-Wales area

The many abandoned base metal mines of the mid-Wales ore field are sources of extensive pollution. Some of the mineralised veins contain large amounts of pyrite and marcasite and oxidative weathering of these produces sulphuric acid resulting in very acidic mine drainage waters. In addition, the spoil tips associated with these mines can contain abundant iron sulphides. Drainage waters from these sources have pH values as low as 2.6 and are heavily contaminated with metals such as Al, Zn, Cd and Ni.Two of the main rivers of the area, the Rheidol and Ystwyth, intercept heavily contaminated acidic drainage which has a marked effect on water quality. The Rheidol contains over 100 g L^–1 Zn for 16 km downstream of the acid water influx. This level is over three times the recommended EEC limit for Zn in salmonoid waters of low hardness.