Identifying fecal sources in a selected catchment reach using multiple source-tracking tools

Given known limitations of current microbial source-tracking (MST) tools, emphasis on small, simple study areas may enhance interpretations of fecal contamination sources in streams. In this study, three MST tools-Escherichia coli repetitive element polymerase chain reaction (rep-PCR), coliphage typing, and Bacteroidales 16S rDNA host-associated markers-were evaluated in a selected reach of Plum Creek in south-central Nebraska. Water-quality samples were collected from six sites. One reach was selected for MST evaluation based on observed patterns of E. coli contamination. Despite high E. coli concentrations, coliphages were detected only once among water samples, precluding their use as a MST tool in this setting. Rep-PCR classification of E. coli isolates from both water and sediment samples supported the hypothesis that cattle and wildlife were dominant sources of fecal contamination, with minor contributions by horses and humans. Conversely, neither ruminant nor human sources were detected by Bacteroidales markers in most water samples. In bed sediment, ruminant- and human-associated Bacteroidales markers were detected throughout the interval from 0 to 0.3 m, with detections independent of E. coli concentrations in the sediment. Although results by E. coli-based and Bacteroidales-based MST methods led to similar interpretations, detection of Bacteroidales markers in sediment more commonly than in water indicates that different tools to track fecal contamination (in this case, tools based on Bacteroidales DNA and E. coli isolates) may have varying relevance to the more specific goal of tracking the sources of E. coli in watersheds. This is the first report of simultaneous, toolbox approach application of a library-based and marker-based MST analyses to flowing surface water.     

Bioavailability of permethrin and cyfluthrin in surface waters with low levels of dissolved organic matter

Pyrethroids are widely used insecticides in both agricultural and urban environments, and their potential movement to surface streams and toxicity to susceptible aquatic species is an emerging concern. Natural surface waters usually contain low levels of dissolved organic matter (DOM). Limited data have shown that DOM preparations can significantly alter the bioavailability and toxicity effects of pyrethroids. However, the importance of these effects in natural waters has not been investigated. In this study we measured uptake and acute toxicity of permethrin and cyfluthrin by Daphnia species in 15 surface water samples. Low levels of DOM (3-20 mg L(-1)) inhibited cyfluthrin uptake by Daphnia magna and acute toxicity to Ceriodaphnia dubia in most samples. For permethrin, the effects of DOM on bioavailability and toxicity were generally not significant. The effects of DOM on bioavailability of cyfluthrin could not be explained from the DOC concentration alone, suggesting that properties of DOM were also important in regulating bioavailability. Regression of K DOC with selected DOM properties revealed significant dependence of K DOC on the carboxylic acid content of DOM. Moreover, concentrations sensed by solid-phase microextraction (SPME) fibers were well correlated with the observed changes in bioaccumulation by D. magna and acute toxicity to C. dubia. Therefore, selective sampling methods such as SPME may be used for measuring the bioavailable concentrations of pyrethroids in waters with naturally occurring DOM levels and predicting the actual toxicity effects.     

Field history and dissipation of atrazine and metolachlor in Colorado

Farmers in eastern Colorado have commented that atrazine does not provide the length of weed control that they expected in fields that have received multiple applications of the herbicide. Multiple laboratory studies suggest that atrazine dissipates more rapidly in soils with a history of atrazine use compared with soils that had not been treated with the herbicide and this could be related to the above observation. Field and laboratory studies were conducted to determine the rate of dissipation of atrazine and metolachlor in fields in Colorado. The published half-lives of atrazine and metolachlor are 60 and 56 d, respectively. In the field studies, the half-lives of atrazine and metolachlor in the top 15 cm of the soil ranged between 3.5 and 7.2 d and 17.9 and 18.8 d, respectively. In laboratory studies, the half-life of atrazine varied from 1.4 to 19.8 d with the shortest half-life occurring in soils which had been treated with atrazine for at least 5 yr. The longest half-life was in a soil that had never received atrazine. The half-life of metolachlor in these same soils varied from 10.6 to 28.2 d. There was no apparent relationship between the half-life of metolachlor and the half-life of atrazine in the laboratory studies. These results confirm farmers' observation of the shorter residual activity of atrazine in Colorado fields receiving atrazine over multiple years.     

Integrating long-term stewardship goals into the remediation process: natural resource damages and the Department of Energy

The United States and other developed countries are faced with restoring and managing degraded ecosystems. Evaluations of the degradation of ecological resources can be used for determining ecological risk, making remediation or restoration decisions, aiding stakeholders with future land use decisions, and assessing natural resource damages. Department of Energy (DOE) lands provide a useful case study for examining degradation of ecological resources in light of past or present land uses and natural resource damage assessment (NRDA). We suggest that past site history should be incorporated into the cleanup and restoration phase to reduce the ultimate NRDA costs, and hasten resource recovery. The lands that DOE purchased over 50 years ago ranged from relatively undisturbed to heavily impacted farmland, and the impact that occurred from DOE occupation varies from regeneration of natural ecosystems (benefits) to increased exposure to several stressors (negative effects). During the time of the DOE releases, other changes occurred on the lands, including recovery from the disturbance effects of farming, grazing, and residential occupation, and the cessation of human disturbance. Thus, the injury to natural resources that occurred as a result of chemical and radiological releases occurred on top of recovery of already degraded systems. Both spatial (size and dispersion of patch types) and temporal (past/present/future land use and ecological condition) components are critical aspects of resource evaluation, restoration, and NRDA. For many DOE sites, integrating natural resource restoration with remediation to reduce or eliminate the need for NRDA could be a win-win situation for both responsible parties and natural resource trustees by eliminating costly NRDAs by both sides, and by restoring natural resources to a level that satisfies the trustees, while being cost-effective for the responsible parties. It requires integration of remediation, restoration, and end-state planning to a greater degree than is currently done at most DOE sites.     

Response to environmental flows in the lower Tarim River, Xinjiang, China: ground water

In China's west since the 1950s large-scale ecosystem degeneration has occurred through water diversions for agricultural purposes. Since 2000, 1038 x 10(6) m(3) of water have been released into the Tarim River with the result that water reached the terminal Taitema Lake for the first time in 30 years. This environmental flow raised water-table levels along 350 km of the river. To assess the response of the water-table, a comparison "pristine" site is compared with downstream monitored sites. The results show huge changes in water-table levels. The study verifies that the water-table is extremely responsive to environmental flows, that strong internal similarities exist along the length of the river, and that the effect on the water-table and hence likely riparian vegetation recovery can be tentatively predicted. The actual impacts of the restoration strategy are less than those originally expected politically, socially and within the scientific community. We make recommendations on more effective release strategies.     

Evaluating farm performance using agri-environmental indicators: recent experiences for nitrogen management in The Netherlands

Intensive agriculture, characterized by high inputs, has serious implications on the environment. Monitoring and evaluation of projects aiming at designing, testing and applying more sustainable practices require instruments to asses agronomic as well as environmental performance. Guidelines for Good Agricultural Practice (GAP) or Good Farming Practice (GFP) define sustainable practices but give limited insight into their environmental performance. Agri-environmental indicators (AEIs) provide information on environmental as well as agronomic performance, which allows them to serve as analytical instruments in research and provide thresholds for legislation purposes. Effective AEIs are quantifiable and scientifically sound, relevant, acceptable to target groups, easy to interpret and cost-effective. This paper discusses application of four AEIs for nitrogen (N) management in three Dutch research projects: 'De Marke', 'Cows and Opportunities' and 'Farming with a future'. 'De Marke' applied Nitrogen Surplus and Groundwater Nitrate Concentration in the design and testing of environmentally sound dairy systems. 'Cows and Opportunities', testing and disseminating dairy systems designed at 'De Marke', mainly applied Nitrogen Surplus, while 'Farming with a future' used Nitrogen Surplus, Groundwater Nitrate Concentration and Residual Mineral Soil Nitrogen to support arable farmers in complying with Dutch legislation (MINAS). Nitrogen Surplus is quantifiable, appealing and easy to interpret, but lacks scientific soundness or a good relationship with groundwater quality. Nitrogen Use Efficiency is sensitive to changes in management, while Residual Mineral Soil Nitrogen is appealing and cheap, but has difficulties in scaling. Groundwater Nitrate Concentration lacks clear rules for sampling, is labor consuming, expensive and mainly used in combination with other indicators. AEIs enhanced improvements in N management by facilitating (i) definition of project goals, (ii) design of desired systems, (iii) evaluation of applied systems and (iv) improving effective communication. AEI applications in other countries show a similar pattern as found in The Netherlands. Limitations to AEI application relate to inconsistencies between different indicators, heterogeneity of soil characteristics and linkages of N, carbon and water management. AEIs should be applied in an integrated evaluation, at a scale that reflects the farm's spatial variability. Simple AEIs like Nitrogen Surplus should be supported by other indicators and/or model calculations. The paper concludes that AEIs proved their value in design, implementation and testing of farming systems, but they should be used with care, always keeping in mind that indicators are simplifications of complex and variable processes.     

Future carbon balance of China's forests under climate change and increasing CO2

The possible response of the carbon (C) balance of China's forests to an increase in atmospheric CO(2) concentration and climate change was investigated through a series of simulations using the Integrated Terrestrial Ecosystem Carbon (InTEC) model, which explicitly represents the effects of climate, CO(2) concentration, and nitrogen deposition on future C sequestration by forests. Two climate change scenarios (CGCM2-A2 and -B2) were used to drive the model. Simulations showed that China's forests were a C sink in the 1990 s, averaging 189 Tg C yr(-1) (about 13% of the global total). This sink peaks around 2020 and then gradually declines to 33.5 Tg C yr(-1) during 2091-2100 without climate and CO(2) changes. Effects of pure climate change of CGCM2-A2 and -B2 without allowing CO(2) effects on C assimilation in plants might reduce the average net primary productivity (NPP) of China's forests by 29% and 18% during 2091-2100, respectively. Total soil C stocks might decrease by 16% and 11% during this period. China's forests might broadly act as C sources during 2091-2100, with values of about 50 g Cm(-2)yr(-1) under the moderate warming of CGCM2-B2 and 50-200 g Cm(-2)yr(-1) under the warmer scenario of CGCM2-A2. An increase in CO(2) might broadly increase future C sequestration of China's forests. However, this CO(2) fertilization effect might decline with time. The CO(2) fertilization effects on NPP by the end of this century are 349.6 and 241.7 Tg C yr(-1) under CGCM2-A2 and -B2 increase scenarios, respectively. These effects increase by 199.1 and 126.6 Tg C yr(-1) in the first 50 years, and thereafter, by 150.5 and 115.1 Tg C yr(-1) in the second 50 years under CGCM2-A2 and -B2 increase scenarios, respectively. Under a CO(2) increase without climate change, the majority of China's forests would be C sinks during 2091-2100, ranging from 0 to 100 g Cm(-2)yr(-1). The positive effect of CO(2) fertilization on NPP and net ecosystem productivity would be exceeded by the negative effect of climate change after 2050. Under the CGCM2-A2 climate scenario and with direct CO(2) effects, China's forests may be a small C source of 7.6 Tg C yr(-1) during 2091-2100. Most forests act as C sources of 0-40 g Cm(-2)yr(-1). Under the CGCM2-B2 climate scenario and with direct CO(2) effects, China's forests might be a small C sink of 10.5 Tg C yr(-1) during 2091-2100, with C sequestration of most forests ranging from 0 to 40 g Cm(-2)yr(-1). Stand age structure plays a more dominant role in determining future C sequestration than CO(2) and climate change. The prediction of future C sequestration of China's forests is very sensitive to the Q(10) value used to estimate maintenance respiration and to soil water availability and less sensitive to N deposition scenario. The results are not yet comprehensive, as no forest disturbance data were available or predicted after 2001. However, the results indicate a range of possible responses of the C balance of China's forests to various scenarios of increase in CO(2) and climate change. These results could be useful for assessing measures to mitigate climate change through reforestation.     

Managing the Use of Copper-Based Antifouling Paints

Copper is the biocide of choice for present-day antifouling (AF) paints. It is also a major source of copper loading in to the marine environment and, as such, might cause local copper levels to exceed water quality criteria. The present study is multifaceted and looks into the overall impact of copper-based AF paints on copper concentrations along a 64-km stretch of the Indian River Lagoon and at Port Canaveral, Florida. This preliminary study is one of the first to outline issues and present background evidence on the current status of copper and copper-based AF usage in Florida and to address the need for management. Previous measurements of copper levels in these waters show a history of copper contamination close to marinas, boatyards, and at Port Canaveral that often exceed state and federal water quality standards. Further, we estimate that the total annual copper input into the Indian River Lagoon is between 1.7 tons/year (sailboats) and 2.1 tons/year (powerboats) from boats in 14 marinas. We estimate the copper input into Port Canaveral to be about 1.4 tons/year from seven cruise ships. A brief survey of marina operators and boat owners revealed attitudes and practices associated with AF paint usage that ranged from excellent to inferior. Management recommendations are made for a proactive approach to improving AF paint selection and application, assessing the environmental status of copper, and redefining existing management practices for sustainable AF paint usage and environmental health.     

The effects of sediment-laden waters on irrigated lands along the lower Yellow River in China

Expansion of irrigation in the Yellow River (Huang He in Chinese) Basin of China is a major accomplishment of the post-revolutionary period in China. Irrigation reliance on the Yellow River was anticipated to not only supply greater reliability of water for crops, but also to improve the productivity of aeolian, saline and alkali soils because of the high sediment loads in the river. Irrigation expansion also was a significant factor in affecting human modification of the landscape ecosystem in the lower reaches of the Yellow River. Based on field investigation and sampling of the amount and distribution of used suspended sediment load in irrigated areas, this paper analyzes the impact of the suspended sediment on soil texture, fertility and salinity and the consequences to the landscape ecosystem. Results indicate that soil quality has indeed been improved through irrigation and related deposition of sediment, but some local problems created by long periods of irrigation should not be ignored in the future.     

Assessing the economic impact of invasive species: the case of yellow starthistle (Centaurea solsitialis L.) in the rangelands of Idaho, USA

Yellow starthistle (Centaurea solsitialis L.) is an invasive weed that creates problems for the management of Idaho's rangelands. A bioeconomic approach combined with an input-output economic model is used to estimate direct and secondary economic costs of the weed in relation to its interference with agricultural and non-agricultural benefits that rangelands provide. Direct economic costs of the infestations were estimated to be of 8.2 million '05 dollars per year, and secondary costs of 4.5 million '05 dollars per year, for a total of 12.7 million '05 dollars; agricultural related economic impacts accounted for 79% of this total cost, and non-agricultural for 21%.