Prenatal diagnosis of a rare heteropagus

The prenatal diagnosis by ultrasound of an unusual case of supernumerary head is reported. The problems of differential diagnosis, the pathological findings after voluntary interruption of the pregnancy, and the problems of obstetric management are presented.     

The safety of fetoscopy: (I). Effect of umbilical vessel puncture on the fetal heart rate and cord histology

The fetal heart rate (FHR) was continuously monitored during 42 umbilical vessel punctures performed at the placental insertion of the cord in 24 diagnostic fetoscopies in which pure fetal blood was obtained. In only one patient did a deceleration first appear during puncture and aspiration of fetal blood. In two patients decelerations preceded fetoscopy and in two others they began during the fetoscopy but before puncture of an umbilical vessel. In 19 patients, the FHR did not change at all during the procedure. Fetal haemorrhage after sampling was either absent or minimal. Six pregnancies were terminated because a positive diagnosis had been made and 18 healthy babies were born. Umbilical cords were examined after 7 terminations of pregnancy and after 6 deliveries. In the former group the puncture could just be seen with the naked eye and the needle track was demonstrated histologically in 6. No traces of the puncture or other abnormalities were found in the cords after delivery. Fetal blood sampling from umbilical cord vessels, particularly at the placental insertion of the cord, is the technique of choice since pure fetal blood can be obtained without increasing the risk of fetoscopy.     

Tectonic and lithological constraints on the evolution of the Karoo graben of northern Malawi (East Africa)

The results of a lithostratigraphic, tectonic and kinematic study of the Karoo deposits of northern Malawi are reported. The objective of the lithostratigraphic study is to correlate the deposits of the Karoo basins of northern Malawi with the well-known deposits of southern Tanzania, thus establishing a stratigraphic framework through which the timing of faulting can be constrained. The kinematic analysis of faulting constrains the opening direction for the Karoo graben in this area and provides basic data to discuss the Karoo graben development within the regional tectonic framework of south-eastern Africa. The studied faults are defined by moderately to steeply dipping cataclastic zones with a width of up to 15?m and are characterized by an array of slickensided fault surfaces with different orientations and slip directions. In this study, small faults (offset <10?m) and meso-scale faults (offset >10?m, but generally not exceeding 30–40?m) have been distinguished. Methods used to analyse the kinematic data include the 'pressure tension' (PT) method, which estimates the principal axes for the bulk brittle strain, and the internal rotation axis (IRA) method, which estimates the axis of bulk internal rotation and the overall sense of slip at the faults. A mass balance calculation reveals a volume increase of up to 16% during cataclastic deformation in the fault zones. The PT method shows an approximately east trending extension direction for faults that occur only in the latest Carboniferous (?) and Early Permian strata, whereas the fault kinematics from faults that cut middle Permian to Early Triassic rocks is characterized by a ESE to SE trending extension direction. The small faults yield essentially the same kinematic results as the meso-scale faults. In a transport-parallel cross-sectional view, the principal extension axes are at an acute angle of approximately 60° to the major fault planes. Given the moderate fault density, the relatively high angle between the orientation of the principal extension axis and the fault planes suggest only a moderate amount of horizontal extension across the Karoo graben of northern Malawi. Riedel structures in the fault zones formed within two conjugate sets of localized shear zones; slip on one set was top to the W/NW and, on the other, top to the E/SE. The two conjugate sets of Riedel structures have an acute angle about the regional shortening axes, implying that no pronounced rotation of the strain axes occurred. The internal rotation axes for the Riedel structures reveal a largely bimodal distribution and inferred weakly monoclinic to orthorhombic symmetry. Therefore the overall deformation during Karoo rifting in northern Malawi is interpreted to be close to a coaxial deformation with a limited amount of horizontal extension.     

Prenatal diagnosis of 21-hydroxylase deficiency by rflp analysis of the 21-hydroxylase,complement C4, and HLA class II genes

In 19 pregnancies at risk for 21-hydroxylase deficiency (21OHD) in 18 families with at lea one affected child, prenatal diagnosis was performed by RFLP analysis using the enzymi Taq I and EcoRI and the DNA probes specific for the 21 OH genes, the closely linke complement C4 genes and the highly polymorphic HLA class II genes DRB, DQB, and DPI For fetal DNA analysis either chorionic villi or cultivated amniotic cells were used. In all 1 cases, a clear prenatal diagnosis was possible either with the 21OH probe alone or in mo cases, by combining the results of the different closely linked loci.     

Zelluläre Wirkungen der ultravioletten Komponente des Sonnenlichts

The discussion on the possible increase of solar UV on earth due to the destruction of the stratospheric ozone has led to a renewed interest in the action of ultraviolet radiation on biological systems. The paper deals with changes at the cellular level stressing particularly molecular alterations in deoxyribonucleic acid, the carrier of genetic information. The most important repair processes by which lesions are removed or bypassed are described. It is also discussed whether the effectivity of a complex spectrum can be predicted on the basis of measurements with monochromatic radiation. Furthermore, possible consequences on human health are outlined which may be derived from cellular studies.     

Mating System and Sexual Selection in the Scorpionfly Panorpa vulgaris (Mecoptera: Panorpidae)

Panorpa vulgaris   has become a model insect for testing theories of sexual selection. This contribution summarizes that which has been learned in recent years and presents new data that clearly show that the mating system of P. vulgaris is not simply a resource-defense polygyny, as has previously been thought. In P. vulgaris neither the pattern in food exploitation nor the ratio of variance in the lifetime reproductive success of the two sexes is in accordance with that expected in resource defense polygynous mating systems. Lifetime mating duration is the most important proximate determinant of male fitness. Males employing alternative mating tactics obtain copulations of varying duration in relation to the following sequence: saliva secretion  1  food offering  1  no gift. The number of salivary masses which males provide to females during their lifetime is significantly correlated with the lifetime condition index. The condition index depends on the fighting prowess of males and their ability to find food items. Thus saliva secretion of Panorpa is considered a Zahavian handicap, which can serve as an honest quality indicator used by mating females. Our results confirm four main predictions of the indicator model of the theory of sexual selection: (a) the indicator signals high ecological quality of its bearer, (b) the indicator value increases with phenotypic quality, (c) the indicator value is positively correlated with the genetic quality affecting offspring fitness in a natural selection context, and (d) the quality indicator is more costly for low- than for high-quality individuals. The evolutionary consequences of the mating pattern and the sperm competition mechanism in P. vulgaris are discussed in the context the way in which sexual selection creates and maintains sperm mixing and the evolution of a promiscuous mating system.