A contribution for the evaluation of the territorial impact of transport infrastructures in the early stages of the EIA: application to the Huelva (Spain)–Faro (Portugal) rail link

The Environmental Impact Assessment (EIA) procedure should predict and identify the major impacts of a project development that may cause specific spatial and temporal effects. Early in the EIA, the scoping stage provides all the relevant information on the impacts of the project alternatives. In particular, potential effects on the territorial network such as habitat connectivity loss and accessibility improvements should be taken into account in the various proposed layout alternatives when evaluating transport infrastructure projects. However, several authors have identified deficiencies in practice. The aim of this article is to provide a methodology for the assessment of these territorial impacts using adequate indicators in the early stages of the EIA procedure.

The proposed method is based on a comparison of a range of alternative layouts for a railway line linking two population centres, using indicators calculated with geographic information systems. The methodology was applied to a case study – the rail link between Huelva (Spain) and Faro (Portugal) – and the high speed rail (HSR) and conventional rail were evaluated in different layouts. The method was effective in spatially identifying significant impacts on accessibility improvements, which occurred closer to the cross border area. The conventional railway alternatives have similar accessibility values to the HSR. The results also reveal that connectivity loss is not limited to the area around the infrastructure, but extends throughout the territory. The results are at variance with the initiative proposed by Spanish and Portuguese transport decision-makers, and raise the possibility of selecting a conventional railway option. An adequate territorial evaluation methodology enables the new action to be correctly assessed, and supplies the information required to propose the most suitable alternative from a socio-economic and environmental standpoint, regardless of whether this proposal was initially included in the transport policy.     

Spatial variability of sediment ecotoxicity in a large storm water detention basin

Detention basins are valuable facilities for urban storm water management, from both the standpoint of flood control and the trapping of pollutants. Studies performed on storm water have shown that suspended solids often constitute the main vector of pollutants (heavy metals, polycyclic aromatic hydrocarbon (PAH), etc.). In order to characterise the ecotoxicity of urban sediments from storm water detention basins, the sediments accumulated over a 6-year period were sampled at five different points through the surface of a large detention basin localised in the east of Lyon, France. A specific ecotoxicological test battery was implemented on the solid phase (raw sediment) and the liquid phase (interstitial water of sediments). The results of the study validated the method formulated for the ecotoxicological characterization of urban sediments. They show that the ecotoxicological effect of the sediments over the basin is heterogeneous and greater in areas often flooded. They also show the relationship between, on one hand, the physical and chemical characteristics of the sediments and, on the other hand, their ecotoxicity. Lastly, they contribute to a better understanding of the dynamics of the pollution close to the bottom of detention basins, which can be useful for improving their design. The results of this research raise particularly the issue of using oil separators on the surface of detention basins.     

Metal mobilization in soil by two structurally defined polyphenols

Polyphenols including tannins comprise a large percentage of plant detritus such as leaf litter, and affect soil processes including metal dynamics. We tested the effects of tannins on soil metal mobilization by determining the binding stoichiometries of two model polyphenols to Al(III) and Fe(III) using micelle-mediated separation and inductively coupled plasma optical emission spectroscopy (ICP-OES). By fitting the data to the Langmuir model we found the higher molecular weight polyphenol (oenothein B) was able to bind more metal than the smaller polyphenol (epigallocatechin gallate, EGCg). For example, oenothein B bound 9.43 mol Fe mol^?1, while EGCg bound 4.41 mol of Fe mol^?1. Using the parameters from the binding model, we applied the Langmuir model for competitive binding to predict binding for mixtures of Al(III) and Fe(III). Using the parameters from the single metal experiments and information about polyphenol sorption to soils we built a model to predict metal mobilization from soils amended with polyphenols. We tested the model with three natural soils and found that it predicted mobilization of Fe and Al with r² = 0.92 and r² = 0.88, respectively. The amount of metal that was mobilized was directly proportional to the maximum amount of metal bound to the polyphenol. The secondary parameter in each model was the amount of weak organically chelated Fe or Al that was in the soil. This study provides the first compound-specific information about how natural polyphenols interact with metals in the environment. We propose a model that is applicable to developing phytochelation agents for metal detoxification, and we discuss how tannins may play a role in metal mobilization from soils.     

Isolation of five Rubrobacter strains from biodeteriorated monuments

In the last few years, the microbial colonisation of mural paintings in ancient monuments has been attracting the attention of microbiologists and conservators. The genus Rubrobacter is commonly found in biodeteriorated monuments, where it has been reported to cause rosy discolouration. However, to date, only three species of this genus have been isolated, all from thermophilic environments. In this paper, we studied three monuments: the Servilia and Postumio tombs in the Roman Necropolis of Carmona (Spain), and Vilar de Frades church (Portugal), in search of Rubrobacter strains. In all cases, biodeterioration and the formation of efflorescences were observed, and five Rubrobacter strains were isolated. These isolates showed different physiology and migration in denaturing gradient gel electrophoresis, suggesting they might represent new species within this genus. The isolates reproduced some biodeterioration processes in the laboratory and revealed their biomediation in crystal formation.     

Formulating an ecosystem approach to environmental protection

The U.S. Environmental Protection Agency (EPA) has embraced a new strategy of environmental protection that is place-driven rather than program-driven. This new approach focuses on the protection of entire ecosystems. To develop an effective strategy of ecosystem protection, however, EPA will need to: (1) determine how to define and delineate ecosystems and (2) categorize threats to individual ecosystems and priority rank ecosystems at risk. Current definitions of ecosystem in use at EPA are inadequate for meaningful use in a management or regulatory context. A landscape-based definition that describes an ecosystem as a volumetric unit delineated by climatic and landscape features is suggested. Following this definition, ecosystems are organized hierarchically, from megaecosystems, which exist on a continental scale (e.g., Great Lakes), to small local ecosystems.Threats to ecosystems can generally be categorized as: (1) ecosystem degradation (occurs mainly through pollution) (2) ecosystem alteration (physical changes such as water diversion), and (3) ecosystem removal (e.g., conversion of wetlands or forest to urban or agricultural lands). Level of threat (i.e., how imminent), and distance from desired future condition are also important in evaluating threats to ecosystems. Category of threat, level of threat, and distance from desired future condition can be combined into a three-dimensional ranking system for ecosystems at risk. The purpose of the proposed ranking system is to suggest a preliminary framework for agencies such as EPA to prioritize responses to ecosystems at risk.     

Kinship recognition and grouping in hatchling green iguanas

Summary The research addressed two questions: (1) doIguana iguana hatchlings recognize their kin, and (2) what are the recognition mechanisms? Eggs were collected from wild and captive-raised females and hatched in semi-natural incubators. In the first experiment we used eggs from two females. One of the clutches was subdivided to be placed in different incubators. Immediately after emergence one of these hatchling groups was introduced into a 10 m x 10 m enclosure. At the same time we introduced hatchlings from another female. The remaining hatchling group was kept separate for 3 weeks and was then introduced into the same cage. Unrelated hatchlings separated from each other. Related but separated hatchlings grouped according to introduction date. In the second experiment we maintained eight kin groups in individual cages and two hatchlings per kin group in an additonal cage. Hatchlings from the mixed group showed no preference for their kin before physical contact with pure kin groups had occurred. After physical contact hatchling recognized their kin by feces smell. Body odor is likely to be also involved.     

Tolerance to trichlorophenols in microorganisms from a polluted and a pristine site of a river

The effect of 2,4,5- and 2,4,6-trichlorophenol on the microbiota from a polluted and a pristine site of a river was studied. Bacterial metabolic activity measurements by epifluorescence microscopy showed that the polluted site contained more metabolically active cells than the pristine site. Total culturable bacterial counts and tolerant bacterial counts from both sites were not affected by incubation (for up to 5 days) with 200 ppm of chlorophenols. However, the incubation with 500 ppm of 2,4,5-trichlorophenol prevented detection of total and tolerant bacterial counts in the pristine site, and inhibited tolerants in the polluted site. None of 250 bacterial colonies directly isolated from these samples was able to grow on chlorophenols. However, bacteria able to grow on 2,4,6-trichlorophenol, were obtained by enrichment of water and sediments samples.     

Organochlorine and heavy metal contamination in the eggs of the Spanish Imperial Eagle (Aquila (heliaca) adalberti) and accompanying changes in eggshell morphology and chemistry

Eggs of the Spanish Imperial Eagle (Aquila (heliaca) adalberti) contained small amounts of heavy metals and organochlorine contaminants, including DDE, aldrin, dieldrin and HCHs, PCBs. Levels of organochlorine, except DDE, have decreased from 1972 to 1984. This paper reports how the structural and chemical characteristics of modern shells differ from those of older shells, unaffected by pesticides. Also, fertile and infertile recent eggs are compared. A 12.6% decrease in eggshell thickness has occurred in conjunction with a slight decrease in degree of crystallisation, but no significant changes have been found in other structural variables. The difference in chemical composition of the shell between modern and old eggs is both qualitative (presence of chlorine, potassium, magnesium and copper in recent shells) and quantitative (increase in the percentage of phosphorus and sulphur). The only clear differences between fertile and infertile eggs were a greater increase of phosphorus content and altered crystallisation in the latter. No clear relation between the amount of the mineral constituents of the shell and morphological alterations was established. The levels of contaminants in the contents seemed to be independent of the chemical alterations of the eggshell. The breeding parameters of the overall population showed no significant changes after the introduction of pesticides. However, eggshell changes have occurred in this species, which is one of the rarest birds of prey of the world, and some breeding individuals have sufficiently thin shells to suspect reduced reproductive success.     

Dermal exposure to pesticides in greenhouses workers: discrimination and selection of variables for the design of monitoring programs

Dermal exposure to pesticides is one of the main sanitaryproblems which greenhouses workers face. With the dual aimsof establishing both the body part that receives the greatestexposure and the variable that has greatest influence on this exposure level, 22 pesticide application trials were performed. Trials were carried out in different greenhouse vegetable crops,using different pesticides and different spray diameters from the spray gun. In order to determine dermal exposure, the wholebody method was used. Pieces of the applicator suit were subject to an extraction procedure and their pesticide contentdetermined using GC-NPD analysis. Multivariate analysis were applied to the data obtained. Principal component analysis showed that all trials produced a high exposure level on lowerleft leg and lower right leg. Cluster analysis distinguished between three sample groups. The most and the least affectedparts were clearly distinguished. Discriminant analysis indicated that the thin drop size of the spray gun is responsible for both the differences between groups and the minimum or maximum exposure level measured on the applicatorsuit. Therefore, selecting the variables, lower legs and thindrop size, is considered fundamental in designing programs formonitoring pesticide exposure.     

Evaluation of Satellite‐Derived Rainfall Data for Multiple Physio‐Climatic Regions in the Santiago River Basin,Mexico

Assessment of water resources requires reliable rainfall data, and rain gauge networks may not provide adequate spatial representation due to limited point measurements. The Tropical Rainfall Measuring Mission (TRMM) provides rainfall data at global scale, and has been used with good results. However, TRMM data are an indirect measurement of rainfall, and therefore must be validated for its proper use. In this work, a validation scheme was designed and implemented to compare the TRMM Version 7 (V7) monthly rainfall product at different time frames with data measured in two hydrologic subregions of the Santiago River Basin (SRB) in Mexico: Río Alto Santiago and Río Bajo Santiago (RBS). Additionally, three physio‐climatic regions provide an assessment of the interplay of topography, distance from coastal regions, and seasonal weather patterns on the correspondence between both datasets. The TRMM V7 rainfall product exhibited good agreement with the rain gauge data particularly for the RBS and for the whole SRB during wettest summer and autumn seasons. However, strong regional dependence was observed due to differences in climate and topography. Overall, in spite of some noted underestimations, the monthly TRMM V7 rainfall product was found to provide useful information that can be used to complement limited monitoring as is the case of RBS. An improved combined rainfall product could be generated and thus gaining the most benefits from both data sources.