人类无精症相关新基因ZNF313启动子的初步分析

采用PCR方法扩增了ZNF313基因的启动子序列,构建了含人ZNF313基因启动子不同片断的荧光素酶报告基因表达体系.以pRLTK为内参照质粒,瞬时转染HEK293T细胞,48h后收集细胞,测定荧光素酶的相对表达活性.结果发现,在ZNF313基因的启动子区域构建了4种荧光素酶报告基因表达体系,即pGL3215(-215bp～ 38bp)、pGL3160(-160bp～ 38bp)、pGL3133(-133bp～ 128bp)和pGL38(-8bp～ 128bp).其中pGL3215表达载体的荧光素酶相对表达活性最高;pGL3160和pGL3133表达载体的荧光素酶相对表达活性几乎相同,且是pGL3215的75%;而pGL38的荧光素酶相对表达活性急剧下降,接近于零.这表明,-133bp～-8bp区域内含有人ZNF313基因转录所必需的启动子序列.生物信息学的分析表明,两个SP1、一个AP2和一个TAg是人ZNF313基因启动子所必需的.图4参19     

国林大酒店CI形象策划研究--MI策划部分

一家酒店发展前景与这家酒店现状息息相关，酒店发展需在现实基础上扬长避短，也就需要对现状因素进行整合，使之形成推动酒店发展的合力．于是首先对国林大酒店现状进行即时调查，并进行SWOT分析，在此基础上进行了酒店MI设计．     

长江三角洲开发区建设与发展的比较研究

长江三角洲是我国沿海规模最大的经济区 ,上海市、江苏沿江的南京、镇江、扬州、泰州、苏州、无锡、常州、南通等八市、浙江省的嘉兴、湖州、杭州、宁波、绍兴、舟山等六市都属于长江三角洲经济区的范围 ,土地总面积 996 87.5km2 ,占全国土地总面积的 1%。 1998年全区人口 74 4 5 .6 9万人 ,GDP总量 12 70 1.39亿元。长江三角洲是我国经济最发达 ,人口分布最密集 ,城市化水平最高的地区之一。阐明了长江三角洲开发区建设和发展的基本特征。指出 ,长江三角洲是我国对外开放最早 ,开发区建设数量最多 ,类型最齐全的地区 ,它拥有经济技术开发区、高新技术开发区、农业现代化园区、旅游度假区和保税区等多种类型。经过多年来的开发建设 ,长江三角洲开发区投资的基础设施环境和社会软环境都很好 ,在招商引资方面取得了巨大成就。这些开发区已经成为了其地方经济新的增长点 ,新的制造业基地 ,高科技发展基地 ,外商投资和跨国公司的集聚地。通过对上海、江苏、浙江各种类型开发区的外商投资规模、产业经济结构、城市建设功能和外向型经济格局的比较 ,揭示了长江三角洲开发区建设和发展的区域差异。明确了上海开发区建设在长江三角洲的龙头地位 ,分析了浙江省开发区建设落后于江苏省的主要原因 ,总结出了愈靠近     

珠海市南水沥水(船)闸软土地基处理方法探讨

介绍了珠海市南水沥水(船)闸软土地基的工程地质特性及物理力学性质,对比分析了粉喷桩、预制桩或钻孔灌注桩等地基处理方式的优缺点,重点介绍了粉喷桩的设计要点、设计计算的方法以及地基处理时应注意的问题,可为类似的水利工程建设提供借鉴。     

PW_(12)/SiO_2光催化降解水中苯酚

采用溶胶-凝胶法制备了二氧化硅负载H3PW12O40催化剂,并采用红外光谱、XRD及BET对其进行了表征。研究了其对水中苯酚的光催化降解性。讨论了负载催化剂用量、光照时间对苯酚和TOC去除率的影响。结果表明:PW12/SiO2负载型催化剂用量为3g/L,光照6h,10mg/L苯酚水溶液中苯酚去除率为93.06%,TOC去除率为73.20%。PW12/SiO2多相光催化10mg/L苯酚水溶液降解反应和苯酚水溶液直接光解反应均为一级动力学反应。     

基于GaN技术的大功率T/R组件可靠性设计与分析

结合T/R组件的工作原理,对影响大功率T/R组件可靠性的关键技术进行了设计与分析。通过与现有的基于GaAs技术的T/R组件设计电路对比分析,阐述了基于Ga N技术的大功率、高可靠性T/R组件的电路设计方法。     

Electrochemical dechlorination of chloroform in neutral aqueous solution on palladium/foam-nickel and palladium/polymeric pyrrole film/foam-nickel electrodes

Electrochemical dechlorination of chloroform in neutral aqueous solution was investigated using palladium-loaded electrodes at ambient temperature. Palladium/foam-nickel （Pd/foam-Ni） and palladium/polymeric pyrrole film/foam-nickel （Pd/PPy/foam-Ni） composite electrodes which provided catalytic surface for reductive dechlorination of chloroform in aqueous solution were prepared using an electrodepositing method. Scanning electron microscope （SEM） micrographs showed that polymeric pyrrole film modified the electrode-surface characteristics and resulted in the uniform dispersion of needle-shaped palladium particles on foam-Ni supporting electrode. The experimental results of dechlorination indicated that the removal efficiency of chloroform and current efficiency in neutral aqueous solution on Pd/PPy/foam-Ni electrode could be up to 36.8% and 33.0% at dechlorination current of 0.1 mA and dechlorination time of 180 min, which is much higher than that of Pd/foam-Ni electrode.     

室内空气中挥发性有机物污染研究现状

随着经济的发展和人们生活水平品质的提高,室内空气污染逐渐成为人们关心的环境问题之一。文章在查阅了大量文献及结合作者工作的基础上,介绍了室内空气中挥发性有机物污染水平研究现状,采样分析方法和治理技术的进展情况。     

世博园区水体底泥氮磷分布特征

分析世博园区水体底泥中氮磷营养盐、硫化物和有机质含量,结果表明,硫化物为(4.51±0.12)mg/kg,有机质为(1.73±0.01)%,氨氮为(206.6±1.2)mg/kg,亚硝酸盐氮为(0.041±0.001)mg/kg,硝酸盐氮为(0.066±0.002)mg/kg,总氮为(0.228±0.002)%,速效磷为(192.3±0.5)mg/kg,总磷为(0.247±0.001)%。运用有机指数评价底泥污染,为0.299±0.005,说明污染较严重。     

Comprehensive evaluation of heavy metal contamination of sediment in Lake Dianchi by using modified AHP method and 137Cs dating

Through the use of general sampling and measurement by ¹³⁷ Cs dating, problems regarding the absence of monitoring data can easily be resolved. Further, weighted values need to be determined while Environment Quality Comprehensive Index (EQCI) is commonly used as applied in environmental quality comprehensive evaluation. In order to overcome the subjectivity in determining weights, the modified Analytical Hierarchy Process (AHP) method was designed. The modified AHP method involved the following key procedures: First, the parameters y _i1 and y _i2 were calculated based on the monitoring data; second, the factors were put in order according to the symbol and value of y _i1 and y _i2; third, the continuous odd integers, which represented the importance of factors, were given to factors according to their seating order; and, fourth, the factor weights were determined from the pair-wise comparison matrix calculated by the ratio of the given odd integers. Therefore, the weights were completely based on the monitoring data. In the present study, the comprehensive quality of sediments in five sections of Lake Dianchi were evaluated and the results indicated that the current contamination of sediments in each lake section is much more serious than at any other time in history. __________ Translated from Environmental Science, 2006, 27(8): 1531–1536 [译自: 环境科学]