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711.
A spore germination-based concept and its transformation into a field level prototype for monitoring aflatoxin M1 (AFM1) in milk was developed. Initially, 15 strains of Bacillus spp. procured from different culture collection were screened for AFM1 sensitivity using spot assay and marker strain showing inhibition at 0.5 ppb was selected based upon maximum zone of inhibition. The selected strain B. megaterium 2949 was further screened for different enzymes activities and subsequently its spores were produced to an extent of 73.13% ± 3.197% in newly developed sporulation medium containing beef extract (0.0075% ± 0.0004%), yeast extract (0.015% ± 0.001%), peptone (0.0375% ± 0.0016%), and sodium chloride (0.0375% ± 0.0018%). A spore germination-based concept/ assay was optimized by immobilizing spores in eppendorf with pretreated milk (80°C/15 min) containing germinant and chromogenic substrate followed by incubation at 37°C. The appearance of sky blue color within real time of 45 min indicated spores germination and release of specific marker enzyme such as acetyl esterase and its specific action on chromogenic substrate which demonstrates absence of AFM1 in milk. However, if there was no color change, presence of AFM1 at 0.5 ppb MRL was denoted by Codex. The developed concept on AFM1 detection was validated and a correlation of 0.97 was established with AOAC approved Charm 6602 and ELISA at Codex MRL with minimal false positive and negative results. The cost effective test has potential application in dairy farms, manufacturing, and R&D units for routine monitoring of AFM1 in milk. 相似文献
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Shaun K. Wilson Nicholas A. J. Graham Rebecca Fisher Jan Robinson Kirsty Nash Karen Chong‐Seng Nicholas V. C. Polunin Riaz Aumeeruddy Rodney Quatre 《Conservation biology》2012,26(6):995-1004
Disturbance plays an important role in structuring marine ecosystems, and there is a need to understand how conservation practices, such as the designation of Marine Protected Areas (MPAs), facilitate postdisturbance recovery. We evaluated the association of MPAs, herbivorous fish biomass, substrate type, postdisturbance coral cover, and change in macroalgal cover with coral recovery on the fringing reefs of the inner Seychelle islands, where coral mortality after a 1998 bleaching event was extensive. We visually estimated benthic cover and fish biomass at 9 sites in MPAs where fishing is banned and at 12 sites where fishing is permitted in 1994, 2005, 2008, and 2011. We used analysis of variance to examine spatial and temporal variations in coral cover and generalized additive models to identify relations between coral recovery and the aforementioned factors that may promote recovery. Coral recovery occurred on all substrate types, but it was highly variable among sites and times. Between 2005 and 2011 the increase in coral cover averaged 1%/year across 21 sites, and the maximum increase was 4%/year. However, mean coral cover across the study area (14%) remained at half of 1994 levels (28%). Sites within MPAs had faster rates of coral recovery than sites in fished areas only where cover of macroalgae was low and had not increased over time. In MPAs where macroalgae cover expanded since 1998 there was no recovery. Where coral was recovering on granite reefs there was a shift in relative prevalence of colony life‐form from branching to encrusting species. This simplification of reef structure may affect associated reef fauna even if predisturbance levels of coral cover are attained. Efecto de la Expansión de Macroalgas y Áreas Marinas Protegidas sobre la Recuperación de Coral Después de una Perturbación Climática 相似文献
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N. N. Nemova E. P. Ieshko O. V. Meshcheryakova N. V. Il’mast L. V. Anikieva D. I. Lebedeva M. V. Churova O. P. Sterligova Ya. A. Kuchko 《Russian Journal of Ecology》2012,43(4):323-327
The population structure and biological parameters of the European whitefish, Coregonus lavaretus (L.), have been analyzed in a technogenically polluted water body. The biochemical state of the whitefish and the fauna and morphology of helminths parasitizing them have been assessed. It is shown that the species composition of the fish, plankton, benthos, and parasite faunas of this water body are depleted and the morphological parameters of common fish parasites are altered. It is noted that, in addition to adaptive rearrangements in biochemical metabolism, the whitefish from the transformed water body are characterized by a reduction in the level of protein synthesis in muscles and the rate of aerobic energy metabolism in the liver and muscles. 相似文献
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Rachèl V. van Schendel G. C. Page-Christiaens Lean Beulen Catia M. Bilardo Marjon A. de Boer Audrey B. C. Coumans Brigitte H. Faas Irene M. van Langen Klaske D. Lichtenbelt Merel C. van Maarle Merryn V. E. Macville Dick Oepkes Eva Pajkrt Lidewij Henneman for the Dutch NIPT Consortium 《黑龙江环境通报》2016,36(12):1091-1098
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V. A. Romanenkov J. U. Smith P. Smith O. D. Sirotenko D. I. Rukhovitch I. A. Romanenko 《Regional Environmental Change》2007,7(2):93-104
The Model of Humus Balance was used to estimate the influence of climate effects and changing agricultural practices on carbon
(C) levels in soddy–podzolic soils in the Russian Federation for the years 2000–2050. The model was linked with a spatial
database containing soil, climate and farming management layers for identification of spatial change of C sequestration potential.
Analysis of relationships between C, soil texture and climate indicated that compared with a business-as-usual scenario, adaptation
measures could increase the number of polygons storing soil organic carbon (SOC) by 2010–2020. The rate of possible C loss
is sensitive to the different climate scenarios, with a maximum potential for SOC accumulation expected in 2030–2040, thereafter
decreasing to 2050. The effect is most pronounced for the arid part of the study area under the emission scenario with the
highest rate of increase in atmospheric CO2 concentration, supporting findings from the dynamic SOC model, RothC. C sequestration during the study period was permanent
for clay and clay loam soils with a C content of more than 2%, suggesting that C sequestration should be focused on highly
fertile, fine-textured soils. We also show that spatial heterogeneity of soil texture can be a source of uncertainty for estimates
of SOC dynamics at the regional scale.
Figures in color are available at 相似文献